MiniBooNE the Booster Neutrino Experiment
小学上册第11次英语第6单元暑期作业(有答案)

小学上册英语第6单元暑期作业(有答案)英语试题一、综合题(本题有100小题,每小题1分,共100分.每小题不选、错误,均不给分)1.The goldfinch's song is pleasant to ______ (听).2. A frog's legs help it swim and ______ (跳).3.I love playing with my ________ (玩具车) on the floor.4.My cousin is always . (我的表兄弟/表姐妹总是。
)5.The process of separating components of a mixture using a filter is called _______.6.The ____ has sharp claws and is very agile.7.He likes to play _______.8.What do you call a young female chimpanzee?A. InfantB. PupC. CubD. Kit答案: A9.The ice cream truck is ______ (coming) down the street.10.The wind blows through the ______ (树叶). It sounds very ______ (宁静).11. A supernova is the explosion of a dying ______.12.ts can grow from __________ (切割). Some pla13.The chemical formula for citric acid is ________.14.The _______ has a variety of colors and shapes.15.I can ______ (识别) different species of birds.16.Chemical energy is stored in the bonds of ______.17.We can _______ (一起) read a book.18.The butterfly starts as a _________ (毛毛虫).19.Mahatma Gandhi led India to independence through ______ (非暴力) resistance.20.The _______ of an object can be affected by the surface it is on.21.The basic building blocks of matter are called _____.22.The ________ (季节性) affects flowering times.23.The ________ is green and leafy.24.I like to customize my ________ (玩具) with stickers.25.The sun rises in the ______ (east).26.__________ (有机化合物) contain carbon and are found in living things.27.The __________ is a famous area known for its artistic contributions.28.My dad enjoys going to the ____ (movies).29.What is the name of the famous river in China?A. YangtzeB. MekongC. GangesD. Indus答案:A30.The ______ is the largest bird that cannot fly.31.The ______ is a symbol of peace.32.The ______ (小鹿) grazes in the open field, enjoying the warm ______ (阳光).33. A _____ can be very bright and is often seen at night.34.The process of using heat to break down a substance is called _______.35.My ________ (玩具名称) inspires me every day.36. A neutral solution has equal concentrations of ______.37.The capital of the Netherlands is _______.38.What is the term for the outer layer of the atmosphere?A. TroposphereB. StratosphereC. MesosphereD. Exosphere答案:D.Exosphere39.I enjoy learning about history, especially __________.40.What do you call the scientific study of the mind and behavior?A. PsychologyB. SociologyC. AnthropologyD. Psychiatry答案: A41.I always help my mom with ______.42.The _____ (trophy) is shiny.43.She likes to play with her ___. (friends)44.They are eating ________ for lunch.45.The _____ (spaceship) Apollo 11 took astronauts to the moon.46.The main source of energy for the Earth is _____.47.She is wearing a cute ___. (outfit)48.Mice are known for being very _________ (敏捷).49.The process of freezing turns a liquid into a __________.50.The symbol for carbon is _______.51.The cat is ________ on the sofa.52.The chemical formula for propane is ______.53.I have a new ___. (computer)54.The ________ was a vital treaty that shaped international relations.55.The _____ (植物保护) is important for biodiversity.56. Wall of China was built to protect against ________. The Grea57.What do we call a person who studies the nature of reality?A. PhilosopherB. ScientistC. MathematicianD. Historian答案: A58. A ____ is a curious animal that explores new places.59.I love to watch the stars at ______ (夜晚). They twinkle like ______ (宝石).60.She is _______ (coloring) a picture.61.What is the name of the famous landmark in Paris?A. Eiffel TowerB. Louvre MuseumC. Arc de TriompheD. All of the above答案: D. All of the above62.What is the name of the famous landmark in Paris?A. Eiffel TowerB. Big BenC. Statue of LibertyD. Colosseum答案:A63.The _______ can provide essential food for animals.64.The ____ is a small rodent that likes to nibble on seeds.65.The chemical symbol for potassium is _______.66.The _______ (猫头鹰) hoots at night.67.The _______ (The Industrial Revolution) revolutionized manufacturing processes.68.My brother is a __________ (创新型人才).69.What do you call the person who writes books?A. AuthorB. EditorC. PublisherD. Journalist答案:A70.The process of changing a liquid to a gas is called __________.71.My cousin is a very good ____ (swimmer).72.The _____ (carnivorous) plants trap insects for nutrients.73.My favorite place is the ______ (海边).74.The garden is _______ (色彩斑斓的).75.The capital of Turkey is _____.76.Which animal is known for its long neck?A. GiraffeB. ElephantC. RhinoD. Hippo答案: A77.The ______ helps us learn about community service.78.My brother is interested in ____.79.The ____ is often seen fluttering around in the garden.80.The ______ (青蛙) can be found in many environments.81.What is the name of the famous British scientist known for his work on gravity?A. Albert EinsteinB. Isaac NewtonC. Charles DarwinD. Stephen Hawking答案:B82. A _______ (蟋蟀) chirps at night.83.______ is the process of breaking down rocks into smaller pieces.84. A ________ (蝴蝶) flutters around and is very colorful.85.The _____ (wind/snow) is blowing.86.The Earth’s surface is constantly changing due to ______ and erosion.87.They are ___ a picture. (taking)88.My pet is a small ____.89.The Earth's layers include the crust, mantle, outer core, and ______ core.90.The parade was ________ (精彩).91.The __________ (历史的激励作用) drive progress.92.I enjoy _____ (tasting) different herbs.93.The __________ (历史的践行) embodies principles.94.What is the name of the famous American author known for "To Kill a Mockingbird"?A. Harper LeeB. F. Scott FitzgeraldC. Ernest HemingwayD. Mark Twain答案: A. Harper Lee95.My cat's _______ (反应) is very quick.96.The iguana changes its ______ (颜色) to blend in.97.The chemical formula for table salt is _______.98. A ________ (树木) is essential for clean air.99.What is the name of the famous landmark in Paris?A. Eiffel TowerB. Big BenC. Statue of LibertyD. Colosseum答案: A100.My friend is a _____ (志愿者) who helps others.。
CN7800系列温度控制器说明书

P-49Reguladores de rampa/meseta de 1⁄16 DINMonitorice y controle las aplicaciones de proceso o temperatura con precisión utilizando los reguladores serie CN7800. La serie CN7800 proporciona pantallas de LED dobles para una indicación local del valor del proceso y el valor del punto de referencia. Los métodos de control incluyen encendido y apagado, PID, ajuste automático y ajuste manual. El control de PID se admite con 64 acciones de control de rampa/meseta. Existen dos salidas de alarma adicionales estándar en la serie CN7800. Las salidas de la alarma pueden configurarse rápidamente utilizando las 13 funciones de alarma incorporadas. El regulador se comunica fácilmente con la interfaz RS485 incorporada.U P antalla doble U A juste automático U E ntrada universalU8 programas de rampa/meseta, 8 segmentos cada uno U F unciones de enlace y repetición programables U C omunicaciones RS485U S oftware gratuito U E stándar de 2 alarmasEspecificacionesEntradas: Termopar, RTD, voltaje de CC o corriente CC Pantalla: D os LED de 4 dígitos, 7 segmentos, 6,35 mm dealto (0,25") (PV: rojo, SV: verde)Resolución: 1,0, 0,1 para termopares (excepto los tipos R,S y B)Precisión: I ntervalo de ±0,25%, ±1 dígito menos significativo Voltaje de suministro: 100 a 240 Vca, 50/60 Hz Consumo de energía: 5 VA máx.Temperatura de funcionamiento: 0 a 50 °C (32 a 122 °F)Copia de seguridad de memoria: Memoria no volátil Especificaciones de salida de control: Relé: SPST , 5 A @ 250 Vca resistivaImpulso de voltaje: 14V , 10 @ -20% (máx. 40 mA) Corriente: 4 a 20 mAAlarmas: SPST, 3 A a 250 Vca resistiva Comunicación: P rotocolo de comunicación RS485MODBUS ® A-5-11/RTUPeso: 114 g (4 onzas)Marco frontal: 48 mm 2 (1,89 pulg 2)Corte del panel: 45 mm 2 (1,77 pulg 2)Máximo grosor de panel: 9,50 mm (0,375")Profundidad del panel: 80 mm (3,15")* Descarga gratuita de software CN7-A disponible en /cn7800OMX-R250 (se vende por separado). Disponible en /cn7800Serie CN7800Completo de serie con manual del operador.Ejemplos de pedidos: CN7823, regulador de salida doble, impulso de CC, salida de relé mecánico y comunicaciones RS485. CN7853, salida doble, 4 a 20 mA, relé, dos alarmas.。
菠萝君BOOSTER MINI 2好不好? 看看众测用户怎么说

菠萝君BOOSTER MINI 2好不好?看看众测用户怎么说作者:来源:《电脑报》2021年第06期上个月,电脑报蛋黄星球推出了菠萝君BOOSTER MINI 2的众测活动,受到了玩家的热烈欢迎。
这款出身于国内筋膜枪大厂的最新机型好不好用?本期我们精选了几个玩家的体验报告,看看他们是怎么说的。
本次有幸能够拿到一款国内筋膜枪行业佼佼者菠萝君推出的新品——BOOSTER MINI 2智能筋膜枪。
传统的筋膜枪大多又厚又重,这款菠萝君BOOSTER MINI 2智能筋膜枪摒弃了传统筋膜枪的厚重外形,小巧轻便但又性能强劲。
产品采用可视化LED屏幕,开启状态下挡位、蓝牙、电量、压力程度等运行信息一目了然。
全机功能集成在一个按键上,操作简单、高效。
在开机状态下,短按多功能按钮,筋膜枪开始工作,每按下一次,将会提高1个挡位的振动强度,屏幕上挡位指示灯会逐级填满,这个过程中指示灯会实时显示当前按压力度,共有三个匹配的力度挡位:一级压力为绿色;二级压力为黄色;三级压力为红色。
在按下第五次多功能按钮后,筋膜枪进入AI模式:输出的动力随按压力度而动态调节,无需换挡,精确地护理身体,这都要归功于菠萝君BOOSTER MINI 2配备的这块集成智能芯片。
菠萝君BOOSTER MINI 2配置了4款按摩头——圆形头、扁平头、子弹头、U形头。
在办公室用圆形头对大腿进行了按摩,启动后感觉整个大腿都被带动了起来,圆弧形的设计不刺激,更多的是震动的舒适。
手臂的按摩我采用了扁平按摩头——有一种挤压的感觉,它的震动范围就要比圆形头小一些,能感觉到被按摩的中心区域效果明显。
子弹头针对性更强,不过力道也不小,能带动整个手臂的震动。
像我们这种久坐办公室的打工人,肩颈特别僵硬,特别使用这款U形按摩头来进行按摩,当接触到皮肤的那一刻,我能感觉我的眼镜上下上下的高频跳动,不一会儿肩膀就感觉到了热量,说明效果还是来得很快,比较推荐!菠萝君BOOSTER MINI 2不光拥有如此高的颜值和强大的硬件性能,同时还配备了蓝牙功能,适配专属的“菠萝君放松”小程序,享受生活,极致放松,为你个性化定制最适合的筋膜枪放松方案。
吸人眼球的12个韩国新锐品牌

吸人眼球的12个韩国新锐品牌作者:来源:《纺织服装周刊》2018年第24期6月20~21日,第10届新锐(创意)设计师独立品牌展在首尔举办。
每次展会都有新兴品牌引起国内外参观者的热议,今年也不例外。
现场有200个品牌展示了具备独特个性的商品,这些让人一见倾心的商品令观众们长久驻足,感到“不来咨询仿佛错过一个亿!”本篇报道带您一探究竟。
NOTKNOWING推出近两个月好评如潮想找最近流行的运动型街头休闲品牌的话,就请看看NOTKNOWING。
设计师金诗恩在今年3月NOTKNOWING品牌上市两个月都不到的时间里,就获得了musinsa、a-land、ztreet、morethanwordz、around the corner等线上渠道,甚至是common ground等线下渠道的好评,是名副其实的“能力者”。
NOTKNOWING最大的竞争优势就是把运动风融入潮流设计中,从复古运动服风格和反叛性运动街头风格中获取灵感,以运动风服饰和反叛精神受到了消费者的喜爱。
ATCLIP承载不同故事的包包如果想要寻找既忠于基本功能、又能在时间长河中经久不衰的包的话,就请移步向ATCLIP的展位。
设计师申友京运营的ATCLIP的最大特点就是从不同的领域获得灵感来进行设计。
2016年推出以来,每一个包的背后都有一个故事,都传达了不同的讯息,承载着不同的价值。
承载韩服留白之美和细节特征的knot包和line包,是该品牌的第一个系列,灵感来自家具设计师Eileen Gray的作品。
此外,以荷兰画家约翰内斯·维米尔作品为灵感,与重新诠释的音乐一起推出的post包和time包,也是该品牌具有代表性的商品。
Ohlady蕴含吴允雅风格的内衣Ohlady是演员吴允雅推出的内衣专业品牌,针对优雅又有独创性、外形知性、感性和理性结合的女性。
今年2月进入时尚市场后,相对简单又不失功能性的内衣契合了潮流,极具人气,这些商品得到了消费者的广泛喜爱。
小学下册第12次英语第二单元测验卷(含答案)

小学下册英语第二单元测验卷(含答案)英语试题一、综合题(本题有100小题,每小题1分,共100分.每小题不选、错误,均不给分)1._____ (草原) are home to many wildflowers.2.Which type of tree produces acorns?A. PineB. MapleC. OakD. Birch答案:C3.What do we call the force that opposes motion?A. GravityB. FrictionC. TensionD. Compression4.The Earth's crust is continuously undergoing ______.5.The _____ (pen/pencil) is on the desk.6.My friend is __________ (值得信赖的).7.What do you call a person who designs buildings?A. ArchitectB. EngineerC. ContractorD. Surveyor答案:A8.What do you call the part of the plant that grows above the ground?A. RootB. StemC. LeafD. Flower答案:B9.What do we call the device used to take pictures?A. CameraB. ProjectorC. MonitorD. Scanner答案:A10.My cat enjoys catching ______ (小虫) in the garden.11.The ancient pyramids were built as ________ (墓葬) for pharaohs.12.She wears _____ (glasses/hats).13.The _______ is important for pollination and growth.14.What do we call the imaginary line that runs from the North Pole to the South Pole?A. EquatorB. LongitudeC. LatitudeD. Meridian答案:D Meridian15.What is the name of the fairy tale character who kissed a frog?A. Snow WhiteB. CinderellaC. The Princess and the FrogD. Rapunzel16.What is the term for a baby rabbit?A. KittenB. PuppyC. BunnyD. Fawn答案:C17.The first successful bone marrow transplant was performed in ________.18.I like to collect __________ after a storm. (雨水)19.I love to _______ (煮饭) on weekends.20.What is the primary ingredient in chocolate cake?A. FlourB. SugarC. CocoaD. Eggs答案:C21.What do you call a person who writes books?A. NovelistB. EditorC. PublisherD. Librarian22.My _____ (表弟) is visiting next week.23.The cat is ___ (chasing/sleeping) a mouse.24.What do you call a person who studies insects?A. BiologistB. EntomologistC. ZoologistD. Botanist答案:B25.The __________ (历史的反击) challenge established norms.26.Animals that are active at night are called __________.27.My cousin has a pet _______ (我表弟有一只宠物_______).28.I enjoy ______ (hiking) on trails.29.What is the capital of Qatar?A. DohaB. Al RayyanC. Al WakrahD. Lusail答案:A30.The _____ (小狗) loves to chase its tail. It is very entertaining! 小狗喜欢追自己的尾巴。
纳米技术雨伞英语作文简单又吸引人

纳米技术雨伞英语作文简单又吸引人全文共3篇示例,供读者参考篇1Nanotechnology Umbrellas: Tiny Tech for a Rainy DayAs a student, some of my favorite inventions are the ones that make everyday life just a little bit easier and more convenient. One groundbreaking new technology that promises to do just that is nanotechnology umbrellas. These aren't your ordinary rain protectors – they utilize cutting-edge science at the nanoscale to keep you dry in an entirely new way. Let me explain what makes these nanotechnology umbrellas so awesome.First, let's talk about what nanotechnology actually is. ThePrefix "nano" means one billionth, so nanotechnology refers to manipulating matter at the molecular level – one billionth of a meter! At this incredibly tiny scale, materials can exhibit wildly different physical, chemical, and biological properties than they do at a larger scale. By controlling matter at the nanoscale, scientists can engineer new materials and products with superior and novel functionalities.So how does this mind-bending nanotechnology get integrated into umbrellas? The key is in the fabric. Traditional umbrella canopies are made from woven materials like nylon or polyester which can absorb water and feel heavy when saturated. Nanotechnology umbrellas, on the other hand, have a canopy made from a special nanofiber fabric.This incredible fabric is created by electrically charging a polymer solution and shooting it onto a grounded collector to form extremely thin fibers – way thinner than a human hair! The resulting nanofiber material has a hugely increased surface area compared to conventional fabrics, giving it some truly incredible properties.For starters, the nanofiber fabric is super hydrophobic, meaning it massively repels water. While an ordinary umbrella may absorb some water and start to feel heavy after extended use in the rain, nanotechnology umbrellas with their hydrophobic canopies remain perfectly dry on the inside and very lightweight. Water quite literally pearls and rolls right off!But the benefits don't stop there. The massive surface area of the nanofibers also gives the canopy material immense breathability. This allows nanotechnology umbrellas to provide amazing airflow and prevent that stuffy, muggy feeling yousometimes get under a regular umbrella on a hot, humid day. The evaporation of any condensation also helps to keep the inner surface dry.Additionally, the extremely fine nanofibers scatter and diffuse light more effectively than conventional fabrics. This results in a "softer" feel to the light coming through the canopy for more comfortable use, reducing glare and harsh shadows. Pretty cool capabilities for such an everyday item!Another huge perk is that these nanofiber canopies are far more durable and resistant to tearing than standard umbrella materials. The unique geometric configuration and intermolecular forces of the nanofibers make them exceptionally strong for their incredibly small diameter. Nanotechnology umbrellas can easily withstand powerful winds that would invert or destroy a regular umbrella.But perhaps the most exciting aspect of nanotechnology umbrellas is the scope for future innovations and added functionalities. Since the nanofibers can have properties tuned at the molecular level, there's huge potential for embedding advanced capabilities right into the umbrella fabric itself.For example, researchers are exploring incorporating nanomaterials that could give the canopy self-cleaningproperties through photocatalysis. Imagine an umbrella that literally cleans itself just from exposure to light! Other possibilities include building in anti-microbial nanoparticles, UV-blocking molecules for sun protection, or even simple circuits and LED lights for fun and safety.From a student's perspective, having a nanotechnology umbrella would be so incredibly useful and convenient. These things are effectively impossible to soak through, so you'd never have to worry about your books, notes, or electronics getting drenched in a downpour on the way to class. The breathability and comfort factors are also hugely appealing, as is the added durability to withstand blustery conditions.Honestly, just the core water-repelling abilities of the nanotechnology fabric alone would make these umbrellas a must-have accessory for any student. Can you imagine never having to shake out and dry a sopping wet umbrella after trudging across campus in a storm? Or not having to juggle armloads of damp textbooks and papers because your umbrella failed? With a nanotechnology umbrella, you could just stroll to class completely dry and unbothered!I'm honestly kind of giddy about how transformative an impact this nanotechnology could have on such a basic,everyday item. The capabilities enabled by nanoscience and nanoengineering are truly mind-blowing. To be able to fundamentally re-imagine and augment the properties of an ordinary umbrella material through nanoscale control is such an awesome feat of human ingenuity.At the same time, I have to admit there's a simplistic charm and beauty in the core concept of a nanotechnology umbrella. It's just taking something as basic as keeping dry and maxing out that single core function through cutting-edge science. By optimizing the fabric to be incredibly hydrophobic yet breathable, strong yet lightweight, nanotechnology achieves the quintessential, Platonic ideal of an umbrella. It's such an elegant nexus of complex science and fundamental practicality.So in summary, I'm a huge fan of nanotechnology umbrellas precisely because they represent the power of emerging nanotechnology to enhance basic, everyday items in radically useful new ways. These aren't gimmicky novelties, but rather profoundly improved re-inventions of something as simple and ubiquitous as an umbrella. Keeping bone-dry in a rainstorm while strolling comfortably is something we all want, and nanotechnology delivers that in spades.Imaging walking across campus in a torrential downpour yet arriving at your next class completely unflustered, your books and notes still crisp and dry. That's the power of nanotechnology in an umbrella! As a student constantly hustling between classes with my hands full, I can't wait for nanotechnology umbrellas to hit the mainstream. Bring on the nanotech rain protection!篇2The Incredible Potential of Nanotechnology UmbrellasAs I rushed out the door last week, umbrella in hand to brave the downpour, little did I realize the humble object sheltering me from the rain represented one of the most cutting-edge and exciting fields of science and technology. That's right - my plain old umbrella was actually a incredible example of nanotechnology in action!You might be wondering - what on earth is nanotechnology? It's a fascinating area that deals with structures, devices and materials on an unbelievably tiny scale - we're talking about materials and objects that are measured in nanometers. One nanometer is one-billionth of a meter! At that tiny size, materials can exhibit Some truly amazing properties that seem almost like science fiction.So how does nanotechnology come into play with my umbrella? Well, many modern umbrellas actually incorporate nanomaterials and nanotechnology that give them fantastic capabilities. Let me break it down for you:Waterproofing PowerThe waterproof coating on many umbrellas these days is made using nanomaterials that make the fabric extremely water-resistant. Basically, the nanoparticles create a thin coating with a very specific surface texture that causes water to bead up and roll right off instead of soaking through. It's like giving your umbrella a ninja-level water-repelling force-field!Umbrella StrengthThose thin little umbrella ribs that support the canopy and allow it to flex without breaking? Many are constructed using carbon nanotubes - cylindrical structures made from carbon atoms that are astronomically strong for their size. Thisnano-reinforcement makes the umbrella ribs incredibly tough and resistant to stress and snapping.UV ProtectionSome fancy umbrellas incorporate nanoparticles into the fabric that actually block harmful UV radiation from the sun'srays. These nanoparticles act like a filter, letting visible light through while stopping UV to protect you from sunburn and skin damage. The ultimate beach umbrella!Self-CleaningThis one is really cool - some nanotechnology umbrellas have a self-cleaning coating made from nanoparticles that react to sunlight. When the umbrella gets wet and is exposed to the sun's rays, the nanoparticle coating causes water to bead up and roll off, taking any dirt or grime with it. Your umbrella practically cleans itself!As you can see, nanotechnology has worked its way into some amazingly useful innovations for the humble umbrella. But the potential doesn't stop there - researchers are working on other incredible nanotechnology applications like:Umbrellas with changeable colors or patterns using electrochromic nanofilmsHighly rigid yet lightweight nanocomposite umbrellas for extreme conditionsUmbrellas that harvest kinetic energy from wind and rain to charge your devicesNanocoatings that eliminate odors or have antibacterial propertiesThe possibilities are endless when you can engineer materials and structures at the nanoscale level. It really opens up a new world of advanced materials and products that could change everything from clothing to cars to computers.Of course, like any new technology, nanotechnology also raises some safety and ethical concerns. Some nanoparticles could potentially be toxic or have environmental impacts that need to be studied. There are also issues around regulating nanomaterials and their use in consumer products. We'll have to keep researching and discussing the responsible development of nanotechnology.But overall, I'm incredibly excited about nanotechnology and all the incredible capabilities it can unlock. Who knew such a simple object like an umbrella could demonstrate such powerful science? The next time the rain starts pouring, I'll be sure to appreciate all the amazing nanotechnology working hard to keep me dry and protected.Aren't you glad you learned a bit about the nanotechnology wonders in your umbrella today? The future of materials science is headed for the nanoscale - and it's going to be revolutionary!篇3The Fascinating World of Nanotechnology UmbrellasHave you ever thought about how amazing it would be to have an umbrella that never gets you wet? One that can repel water like a force field? Well, thanks to cutting-edge nanotechnology, such high-tech umbrellas are becoming a reality!As a student really interested in science and emerging technologies, I find the applications of nanotechnology fascinating. Nanotechnology deals with manipulating matter on an ultra-small scale – we're talking about working with materials on the molecular or atomic level. By precisely engineering nanostructures, scientists can create materials with incredible new properties.One exciting use of nanotechnology is in developing super water-repellent or "superhydrophobic" surfaces. These surfaces are like water's worst nightmare – water droplets literally bounce right off them without being absorbed at all. It's almost like the surfaces are coated with an invisible force field that water can't penetrate.How does it work? Basically, scientists can etch or grow tiny nanostructures on surfaces like glass, metals or fabric. When arranged in the right patterns at the nanoscale level, these nanostructures trap pockets of air that make it incredibly difficult for water to stick. The water molecules basically cluster together into tight beads that easily roll off rather than spreading out and wetting the surface.Pretty cool, right? And that's exactly the principle being used to create advanced nanotechnology umbrellas andwater-resistant clothing that can keep you dry in the pouring rain. Imagine never having to worry about your umbrella leaking or your jacket getting soaked – the water will just bead up and roll right off!One company at the forefront of this "liquid umbrella" technology is ARYS, based in Spain. They use nanotechnology to create rainwear with a drivenrepel coating made up of nanoparticles that craters an air cushion to repel water. Their umbrellas are insanely water-resistant – ARYS claims theirnano-umbrellas can take on monsoon-level rainfall without letting a drop inside.Other companies like Silks are using similar nanotech to make ultra water-repellent business suit fabrics. With these suits,you could get caught in a surprise downpour and still show up at your meeting looking fresh and dry. No more lugging a soggy umbrella into the office!But nanotech water protection goes way beyond just clothing and umbrellas. Scientists are developing self-cleaning, water-proof paints, windshields, roof tiles and more using superhydrophobic nano-coatings. Imagine how much easier cleaning would be if dirt and water couldn't stick to surfaces! Buildings could stay cleaner for longer and you might never have to wash your car again in the rain.Of course, there are still some limitations with current nanotechnology water protection. The nano-surfaces can start to lose their water repellency over time as their nanostructures get worn down. Developing more durable nano-coatings is an ongoing challenge. There are also some concerns about potential environmental impacts if large amounts ofnano-materials end up being released.Still, the future possibilities of nanotechnology for water resistance are incredibly exciting. Maybe we'll soon have entire nano-fabric tents that can get soaked in a storm without letting any moisture in. Or self-drying nano-rubber that instantly shedswater and could be used for all-weather gear. Heck, we might even get self-drying nano-swimsuits one day!For me, playing with hydrophobic surfaces made from different materials was one of the coolest experiments in my chemistry class. We coated different surfaces with nano-particle solutions and got to see firsthand how water would just bead up and roll around without sticking. It really brought thenano-world and its weird water-fearing surfaces to life.I can't wait to get my hands on an advanced nanotech umbrella and test its waterproofing for myself on a rainy day. While I'll always have a soft spot for the classic wooden umbrella design, there's no denying the awesome liquid-shielding performance of these new nano-materials. An indestructible, self-drying umbrella that laughs in the face of even the worst downpour? Sign me up!Ultimately, nanotechnology umbrellas are just the start of a wave of super water-resistant products using nano-engineered surfaces. As researchers unlock more precise control over nanostructures, we'll see this technology spread into countless applications where extreme waterproofing is needed. Who knows, maybe we'll even get to experience a future where nothing can ever really get wet!For a science geek like me, that's a potential nano-future that's hard not to get excited about. Nanotechnology really is making the impossible possible when it comes to mastering and manipulating liquids. An umbrella that shrugs off monsoons may be cool, but it's just the tip of the iceberg for where this technology could lead. The nanotech revolution is here – and it's making everything water-resistant!。
Spark Mini Booster 使用手册 V2.1说明书
Spark Mini Booster English Manual Version 2.1 Table of contents Important Safety Instructions1EMC / EMI2About this manual3Introduction4Setup5Inputs, output, controls61. Power input62. Audio input63. Audio output64. Level knob65. Footswitch6Working with a booster: setup examples7Setup examples8The sound of silence: True Bypass11Frequently asked questions11Technical Specifications12Getting support12Important Safety Instructions Important SafetyInstructions1) Read these instructions.2) Keep these instructions.3) Heed all warnings.4) Follow all instructions.5) Do not use this apparatus near water.6) Clean only with dry cloth.7) Do not block any ventilation openings. I n-stall in accordance with the manufacturer’sinstructions.8) Do not install near any heat sources suchas radiators, heat registers, stoves, or otherapparatus (including amplifiers) that pro-duce heat.9) Do not defeat the safety purpose of the po-larized or grounding-type plug. A polarizedplug has two blades with one wider thanthe other. A grounding type plug has twoblades and a third grounding prong. Thewide blade or the third prong are providedfor your safety. I f the provided plug doesnot fit into your outlet, consult an electricianfor replacement of the obsolete outlet.10) Protect the power cord from being walkedon or pinched particularly at plugs, conve-nience receptacles, and the point wherethey exit from the apparatus. 11) Only use attachments/accessories speci-fied by the manufacturer.12) Use only with the cart, stand, tripod, brack-et, or table specified by the manufacturer,or sold with the apparatus. When a cart isused, use caution when moving the cart/apparatus combination to avoid injury fromtip-over.13) Unplug this apparatus during lightningstorms or when unused for long periods oftime.14) Refer all servicing to qualified service per-sonnel. Servicing is required when the ap-paratus has been damaged in any way,such as power-supply cord or plug is dam-aged, liquid has been spilled or objectshave fallen into the apparatus, the appara-tus has been exposed to rain or moisture,does not operate normally, or has beendropped.WarningDo not expose this equipment to dripping orsplashing and ensure that no objects filled withliquids, such as vases, are placed on the equip-ment.Do not install this device in a confined space.ServiceAll service must be performed by qualified per-sonnel.CautionYou are cautioned that any change or modifi-cations not expressly approved in this manualcould void your authority to operate this device.EMC / EMI EMC / EMIElectromagnetic compatibility / Electromagnetic interferenceThis equipment has been tested and found to comply with the limits for a Class B Digital de-vice, pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference in resi-dential installations. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interfer-ence to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encour-aged to try to correct the interference by one or more of the following measures:– Reorient or relocate the receiving antenna.– ncrease the separation between the equip-ment and receiver.– Connect the equipment into an outlet on a cir-cuit different from that to which the receiver is connected.– Consult the dealer or an experienced ra-dio / TV technician for help.For customers in CanadaThis Class B digital apparatus complies with Ca-nadian ICES-003.Cet appareil numérique de la classe B est conforme à la norme NMB-003 du Canada.About this manualAbout this manualThis manual will help you learn understandingand operating your TC product.This manual is only available as a PDF downloadfrom the TC Electronic website.Of course, you can print this manual, but we en-courage you to use the PDF version, which hasboth internal and external hyperlinks. For exam-ple, clicking the TC Electronic logo in the upperleft corner of each page will take you to the tableof contents.To get the most from this manual, please read itfrom start to finish, or you may miss importantinformation.To download the most current version of thismanual, visit /support/manuals/Enjoy your TC product!Introduction Introduction“Size matters not. Look at me.Judge me by my size, do you?”Yoda(“Star Wars: Episode V –The Empire Strikes Back”)Congratulations on your purchase ofTC Electronic’s amazing Spark Mini Booster!Never judge a book by its cover –or a booster pedal by its size.Spark Mini Booster was designed by musiciansfor musicians to give you an amazing extra toolfor your musical tool box. It’s an extremely ver-satile pedal that will help you get better sounds.The possibilities with Spark Mini Booster are vir-tually endless:– Leave it on all the time,– punch it in from time to time when you needa volume boostor– put it in front of your favorite overdrive / dis-tortion pedal to add more tonal options toyour existing pedal board.The choice is yours!Ready for PrimeTime?PrimeTime™ is a radical new feature that willexcite guitarists around the globe. Spark MiniBooster instantly understands whether you wantthe pedal to be permanently on when you hit thefootswitch, or just for as long as you hold thefootswitch down. Which totally rocks for both“always-on” and “emphasizing passages” situ-ations.Se tup SetupReady…The Spark Mini Booster box should contain the following items:– 1 Spark Mini Booster pedal– 2 rubber feet for “non-velcro” pedalboard mounting– 1 TC Electronic sticker– 1 leaflet about TC’s guitar FX product range.Inspect all items for signs of transit damage. In the unlikely event of transit damage, inform the carrier and supplier.If damage has occurred, keep all packaging as it can be used as evidence of excessive handling force.Set…– Connect a 9 V power supply with the followingsymbol to the DC input socket of Spark MiniBooster.! Spark Mini Booster does not come with apower supply.– Plug the power supply into a power outlet.!Please note that Spark Mini Booster has nobattery compartment. A conventional powersupply is required for operating this product.– Connect your instrument to the I NPUT jackon the right side of the pedal using a ¼“ jackcable.– Connect the OUTPUT jack on the left side ofthe pedal to your amplifier using a ¼“ jack ca-ble.Play!Inputs, output, controlsInputs, output, controls1. Power input This is a standard 5.5 /2.1 mm DC plug (centre = negative). To power up Spark Mini Booster, con-nect a power supply to its power input socket. Spark Mini Booster requires a 9 V power supply providing 40 mA or more (not supplied).To minimize hum, use a power supply with iso-lated outputs.2. Audio input This is a standard ¼“ input jack (mono / TS).Connect your guitar here using a regular ¼“ mono cable.For other setups, see the “Setup examples” sec-tion of this manual.3. Audio output This is a standard ¼“ output jack (mono / TS).Connect this jack to your amplifier using a regu-lar ¼“ mono cable.For other setups, see the “Setup examples” sec-tion of this manual.4. Level knob Use this knob to control the level change when the booster is enabled.Minimum setting will leave the level of your signal unchanged while the maximum setting will give you 20 dBs of boost.5. Footswitch Using the footswitch as a standard on / off switch To turn the pedal on, tap the pedal’s footswitch briefly. To turn the pedal off, tap the footswitch again. This works as with any other pedal on your board.PrimeTime Use the PrimeTime feature for brief solos and licks that last only for a few seconds.To engage PrimeTime, hold down the pedal’s footswitch. To disengage, release the footswitch.During PrimeTime, boost will be on. As soon as you release the footswitch, boost will turn off again.Working with a booster: setup examples Working with a booster:setup examplesSpark Mini Booster is an awesome and versatile tool that can be used in more ways than just one.1. Punch it in from time to time when you need a volume boost.2. Punch it in from time to time when you need a gain boost.3. Leave it on all the time to get the best out of your tube amp.Understanding the difference between sce-narios 1 and 2 is essential when working with Spark Mini Booster.When playing with a distortion pedal or an over-driven amp, you know how turning down the vol-ume knob on your guitar cleans up your guitar sound?Well – guess what happens if you do the oppo-site and turn up your signal even more with the booster? Right: more drive is what happens! Refer to whichever scenario fits your require-ments:“I want a volume boost!”Awesome! Then you want to place Spark MiniBooster after your distortion pedal(s).If you use pedals for your distortion sounds, thenyou will want to place your Spark Mini Boosterdirectly after those pedals.I f you use the distortion of your amplifier, thenyou’ll need to place Spark Mini Booster in the FXloop of your amp – otherwise you’ll end up with again boost as explained in the following section.“I want a gain boost!”Rock on! Place Spark Mini Booster before yourdrive pedals or distorting amp to use it as an ex-tra gain switch for increased gain and sustain. Ifyou set up Spark Mini Booster this way, you canuse it to lift the volume of your clean sound whendrive is disengaged. In other words: If used cor-rectly, this can transform an awesome soundingsingle-channel amp into a ridiculously awesomesounding two-channel amp!“Wait – there’s more!?”“Yep. Always on!”I n many configurations, driving your amp a bitharder can give you better tone. So try leavingSpark Mini Booster on all the time – and youmight find yourself addicted and wanting an ex-tra Spark Mini Booster…Setup examples Setup examplesUsing Spark Mini Booster as a volume boosterModulation, delay &reverb pedalsUsing Spark Mini Booster as a gain boosterModulation, delay &reverb pedalsDrivepedalsUsing Spark Mini Booster in an FX loopFX Loop Send ►Frequently asked questionsThe sound of silence: True BypassHere at TC, we have a simply philosophy: When you are using one of our products, you should hear something great – and if you don’t, you shouldn’t hear it at all. This is why this pedal sports True Bypass. When it is bypassed, it is really off and has zero influence on your tone, resulting in optimum clarity and zero loss of high-end.Frequently asked questions“What are the input / output impedance val-ues for Spark Mini Booster?”Input Impedance is 1 MΩ. Output Impedance is 100 Ω.“Is Spark Mini Booster analog or digital?”The entire signal path of Spark Mini Booster is 100 % analog.“Should I place my Spark Booster in front of the amp, or is it better to run it in the amp’s FX loop?”The placement really depends on what you are looking for. f you place it in front of the amp, the Spark Booster will boost the pre-amp signal in your amp and create a more overdriven sound. I f you put it in the loop, you will boost the volume of your amp as the ped-al will be placed after the pre-amp section. Check out the section of this manual called Working with a booster / Setup examples.“Does Spark Mini Booster have balanced or unbalanced input / output?”Spark Mini Booster has unbalanced I / Os. Use cables with TS jack – i.e., standard instrument cables.Getting supportTechnical Specifications – Maximum Setting: 20 dB– Minimum Setting: 0 dB boost (unity gain)– Bypass mode: True Bypass– Signal Circuitry: 100 % analog– Dimensions (Width x Depth x Height):48 x 48 x 93 mm / 1.9 x 1.9 x 3.7“– Input Connector Type:Standard ¼“ jack – mono / TS– Output Connector Type:Standard ¼“ jack – mono / TS– Power Input:Standard 9 V DC,centre negative> 40 mA (not supplied)Due to continuous development, these speci-fications are subject to change without no-tice.Controls– Level Knob: Amount of boost– Switch: Boost On / off / PrimeTime– PrimeTime:Holding down the footswitch for more than one second will activate PrimeTime for boos-ting essential passages. The boost will disen-gage when you release the footswitch.– Input Impedance: 1 MΩ– Output Impedance: 100 ΩGetting supportIf you still have questions about the product af-ter reading this manual, please get in touch with TC Support:/support/。
AOAC 15.1.04 AOAC Official Method 998.10 Efficacy of Preservation-国外标准规范
15.1.04AOAC Official Method998.10Efficacy of Preservationof Non-Eye Area Water-Miscible Cosmeticand Toiletry FormulationsFirst Action1998Caution:A knowledge of microbiological techniques is required for these procedures.Follow general aseptic and safetyprocedures(1).See Table998.10for the results of the interlaboratory study sup-porting the acceptance of the method.A.PrincipleBacteria,yeast,and mold are grown on laboratory media,har-vested,calibrated,and inoculated into test ing serial di-lutions and plate counts,the numbers of organisms surviving in the test products are determined over time.Products meeting the speci-fied criteria are considered adequately preserved for manufacture and consumer use.Products not meeting criteria are considered in-adequately preserved.B.Apparatus(a)Jars.—2–4oz.wide-mouth,straight-side flint glass ointment jars with linerless metal,polypropylene or Teflon®lined screw caps.(b)Disposable borosilicate glass culture tubes.—16×125mm, with caps.(c)Disposable borosilicate glass culture tubes.—20×150mm, with screw caps.(d)Petri plates.—100×15mm.(e)Sterile2.2mL pipets.(f)Sterile swabs.(g)Glass beads.(h)Sterile gauze.(i)10–20µL inoculating loops.(j)Vortex mixer.C.ReagentsFor convenience,dehydrated media of any brand equivalent in function may be used.Test each lot of medium for sterility and growth-promotion using suitable organisms.(a)Letheen agar.—Contains5.0g pancreatic digest of casein,1.0g dextrose,3.0g beef extract,1.0g lecithin,7.0g polysorbate80, and15.0g agar per L.Prepare according to manufacturer’s directions. Dispense into suitable containers and sterilize by autoclaving at 121E C for15min.Final pH should be7.0±0.2at25E C.Place in45E C water bath until agar is45±2E e for pour plates.(b)D/E neutralizing broth(Dey/Engley).—Contains5.0g pan-creatic digest of casein,2.5g yeast extract,10g dextrose,1.0g so-dium thioglycollate,6.0g Na2S2O3⋅5H2O,2.5g NaHSO3,7.0g lecithin,5.0g polysorbate80,and0.02g bromcresol purple per L. Prepare according to manufacturer’s directions.Dispense9or 9.9mL aliquot into tubes and sterilize by autoclaving at121E C for 15min.Final pH should be7.6±0.2at25E e for aerobic plate count,L,dilutions.(c)Nutrient agar.—Contains5.0g pancreatic digest of gelatin,3.0g beef extract,and15.0g agar per L.Prepare according to manu-facturer’s directions.Dispense into tubes and sterilize by autoclaving at121E C for15min.Final pH should be6.8±0.2at 25E C.Cool in inclined position to form a e for bacterial cul-ture maintenance and inoculum preparation.(d)Y/M agar(yeast/malt extract).—Contains3.0g yeast extract,3.0g malt extract,5.0g peptone,10.0g dextrose,and20.0g agar per L.Prepare according to manufacturer’s directions.Dispense into tubes and sterilize by autoclaving at121E C for15min.Final pH should be6.2±0.2at25E C.Cool in slanted e for yeast culture maintenance and inoculum preparation.(e)Potato dextrose agar(PDA).—Contains200g potato infu-sion,20.0g dextrose,and15.0g agar per L.Prepare according to manufacturer’s directions.Dispense into tubes and sterilize by autoclaving at121E C for15min.Final pH should be5.6±0.2at 25E C.Cool in slanted e for mold culture maintenance and inoculum preparation.(f)0.85%Saline.—Dissolve8.50g NaCl in water and dilute to 1L.Dispense into flasks or bottles and sterilize by autoclaving at 121E C for15min.(g)0.85%Saline with0.05%polysorbate80.—Dissolve8.5g NaCl in water,mix in0.50g polysorbate80,and dilute to1L.Dis-pense into suitable containers and sterilize by autoclaving at121E C for15min.(h)Barium sulfate standard No.2.—(1)Prepare a1.0% BaCl2solution by dissolving1.0g BaCl2⋅2H2O in100mL water.(2)Prepare a1.0%H2SO4solution by mixing1.0mL H2SO4in 100mL water.(3)Mix0.2mL solution,(1),with9.8mL solu-tion,(2),in a screw-capped test tube.Cap tightly and store in dark at room temperature.(i)Barium sulfate standard No.7.—Use solutions from C(h).Mix0.7mL solution,(h)(1),with9.3mL solution,(h)(2),in a screw-capped test tube.Cap tightly and store in dark at room temperature.D.Microorganisms(a)Staphylococcus aureus.—ATCC6538.(b)Staphylococcus epidermidis.—ATCC12228.(c)Klebsiella pneumoniae.—ATCC10031.(d)Escherichia coli.—ATCC8739.(e)Enterobacter gergoviae.—ATCC33028.(f)Pseudomonas aeruginosa.—ATCC9027.(g)Burkholderia cepacia.—ATCC25416.(h)Acinetobacter baumannii.—ATCC19606.(i)Candida albicans.—ATCC10231.(j)Aspergillus niger.—ATCC16404.(Note:Environmental microorganism(s)likely to be contami-nants of concern during product manufacture or use are included as a separate inoculum.Predominant environmental microbes isolated during manufacturing,equipment cleaning,and sanitizing,or from related deionized water systems are used as supplemental test inocula.)For culture revival and maintenance,consult references1and2.E.Product Quality Check(a)Weigh1.0g product into a screw-capped culture tube con-taining9.0mL sterile neutralizing broth to make a1:10dilution.If necessary to disperse product,add10to twenty3mm diameter glass beads to tube.Mix on Vortex mixer until homogeneous.(b)Pipet1.0mL of the1:10dilution into each of4sterile Petri plates.Pour15–20mL sterile molten Letheen agar(45±2E C)into each plate.Mix by rotating plates to disperse the dilution thoroughly. Let solidify.(c)Invert and incubate2plates at35±2E C for48h and2plates at 25±2E C for5days.(d)Count the number of colonies on all plates,add,and multiply by2.5to determine the number of colony forming units per gram (cfu/g)in the product.(e)Save plates to be used for the neutralization validation in M by refrigerating.F.Product Preparation(a)Measure20mL sterile saline into4sterile jars,B(a).Cap tightly and store at room temperature.(b)Weigh20g product into each of4sterile jars,B(a).Cap tightly and store at room temperature.G.Bacterial Inocula Preparation(a)Streak each bacterial culture,D(a)–(h),onto a nutrient agar, C(c),slant.Incubate48h at35±2E C.Wash each slant with5.0mL sterile saline,loosening the culture from the agar surface.Transfer the suspension into a sterile tube.Repeat the wash with second 5.0mL aliquot of bine washes and mix on Vortex mixer to disperse evenly.(b)Adjust each wash with sterile saline to yield a suspension of ca108cfu/mL using a McFarland BaSO4standard No.2,C(h),direct microscopic count,turbidimetry,absorbance,or other method cor-related to an aerobic plate count(APC),L.Perform an APC,L,on each suspension to confirm standardization.H.Fungal Inoculum Preparation(a)Streak C.albicans,D(i),on3slants of Y/M agar,C(d).Incu-bate at25±2E C for48h.Wash each slant sequentially with5.0mL aliquot of sterile saline.Repeat with a second5.0mL aliquot of ster-ile bine washes to produce10mL suspension.Mix on Vortex mixer to disperse evenly.(b)Adjust the wash with sterile saline to yield a suspension of ca 107cfu/mL using a McFarland BaSO4standard No.7,C(i),direct microscopic count,turbidimetry,absorbance,or other method that has been correlated to an APC,L.Perform an APC,L,on the suspen-sion to confirm standardization.(c)Streak A.Niger,D(j),on5slants of potato dextrose agar, C(e).Incubate at25±2E C for10days.Dislodge mold spores by add-ing5.0mL sterile saline containing0.05%polysorbate80to each tube and vigorously rubbing the surface of the agar slant with a ster-ile swab.Repeat with a second5.0mL aliquot in each bine the10washes to produce50mL suspension.Filter into a sterile con-tainer through3–5layers of sterile gauze supported in a funnel.Per-form an APC,L,using appropriate dilutions.Adjust mold suspen-sion to ca107per mL using sterile e immediately or refrigerate at2–5E C for up to1month.Verify mold viability by an APC,L,before each use.I.Inoculum Pools(a)Pool equal parts of the S.aureus and S.epidermidis suspen-sions,G(b),in a sterile container to make Inoculum Pool1: Gram-positive cocci.(b)Pool equal parts of the K.pneumoniae,E.coli,and E. gergoviae suspensions,G(b),in a sterile container to make Inoculum Pool2:Gram-negative fermentors.(c)Pool equal parts of the P.aeruginosa,B.cepacia,and A. baumanii suspensions,G(b),in a sterile container to make Inoculum Pool3:Gram-negative nonfermentors.(d)Pool equal parts of C.albicans,H(b),and A.niger,H(c),sus-pensions in a sterile container to make Inoculum Pool4:Fungi. (e)Use organism pools immediately or refrigerate them at2–5E C for no more than72h.J.Inoculation(a)Inoculate each of the four20.0mL aliquots of sterile saline,F(a), with0.2mL of its respective Inoculum Pool,I(a)–(d).Mix thoroughly. Use these suspensions to determine inoculum counts[see K(a)]. (b)Inoculate each of the four20g product suspensions,F(b), with0.2mL of its respective Inoculum Pool,I(a)–(d).Mix thor-oughly by shaking,Vortex mixing,or stirring,so that each suspen-sion contains106bacteria or105fungi per gram,evenly distributed throughout the product.Tightly close inoculated containers and store at ambient temperature(20–25E C).K.Sampling Intervals(a)Sample each inoculated saline suspension,J(a),for APC,L, within1h after inoculation to obtain inoculum count.(b)Test each inoculated product,J(b),for APC,L,at7,14,and 28days after inoculation to obtain product interval count.L.Aerobic Plate Count(APC)(a)Mix suspension thoroughly.Weigh1.0g product into a screw-capped culture tube containing9.0mL sterile neutralizing broth for a1:10dilution.If necessary to disperse product,add 10–20sterile3mm diameter glass beads to the tube.Mix on Vortex mixer until homogeneous.Table998.10Interlaboratory study results for determination of the efficacy of preservation of non-eye area water-miscible cosmetic and toiletry formulationsProduct name Incidence of false-negatives amongtotal positive samples aSensitivity rateIncidence of false-positives amongtotal negative samples bSpecificity rate Number Percentage Number PercentageShampoo2/494960/530100 Conditioner3/486940/540100 Water in oil emulsion0/5201001/50298 Oil in water emulsion0/5101000/510100 All combined5/2002981/2080.599.5a False-negative analysis indicates a sample is adequately preserved.b False-positive analysis indicates a sample is not adequately preserved.(b)Aseptically pipet0.1mL of the1:10dilution into a9.9mL tube of neutralizing broth to obtain a1:1000dilution.Vortex mix. Pipet0.1mL of the1:1000dilution into9.9mL neutralizing broth to obtain a1:100000dilution.The number of dilutions may be de-creased if previous counts of microbial populations show reduction.(c)Using a2.2mL pipet,aseptically pipet1.0and0.1mL aliquots from the1:10dilution into duplicate Petri dishes for the1:10 and1:100plates.If necessary,transfer duplicate1.0and0.1mL aliquots from the1:1000dilution for plates1:1000and1:10000,and from the1:100000dilution for plates1:100000and1:1000000. Pour15–20mL sterile Letheen agar,C(a),(45±2E C)into each plate.Mix by rotating the plates to disperse the suspension thor-oughly,and let solidify.(d)Invert bacterial plates and incubate at35±2°C.Examine bacterial plates after48–72h.Count plates in a suitable range (30–300colonies).If no countable plates fall in that range,count the plate(s)nearest that range showing distinct colonies.Average dupli-cate plate counts and express results as cfu/g of product.(e)Invert and incubate fungal plates at25±2°C.Read fungal plates at2–3days and record results.Count plates in a suitable range (30–300colonies).If no countable plates fall in that range,count the plate(s)nearest that range showing distinct colonies.Reincubate plates for another2–3days.Read and record additional colonies. Add to previous results to obtain total counts.Average duplicate plate counts and record as cfu/g of product.For information on aver-aging,refer to reference3.M.Neutralization CheckMake a1:10000dilution in sterile saline of Pools1,2,and3, I(a)–(c),and a1:1000dilution of Pool4,I(d).Streak each dilution for isolation with a10µL loop on the plates saved from E(e).If plates are not usable due to either desiccation or surface growth,re-peat section E,and streak freshly prepared plates.Incubate as in L(d)–(e).N.Data Analysis(a)Product quality check,E(d),must be found to contain <100cfu/g to proceed with the challenge test.(b)Inoculum counts,K(a),should be between1to9.9×106cfu/g product for bacteria and1to9.9×105cfu/g product for fungi,or the test should be repeated with different dilutions.(c)Neutralization check,M,must show significant growth of all pools to confirm adequate neutralization.A neutralizing broth other than D/E broth can be used.If neutralization does not occur,the test is invalid.Refer to references4–6for assistance.(d)Calculate the percent reduction:Reduction,%=inoculum count–product interval countinoculum count100(e)The test product is considered adequately preserved if(1)bac-teria show at least99.9%(3log)reduction within1week following challenge and remain at or below that level thereafter,and(2)fungi show at least a90%(1log)reduction within1week following chal-lenge,a99%(2log)reduction within2weeks following challenge, and remain at or below that level thereafter.These criteria apply to freshly prepared formulations.References:J.AOAC Int.84,101(2001).(1)Methods for General and Molecular Bacteriology(1993)P.Gerhardt(Ed.),American Society for Mi-crobiology,1752N St NW,Washington,DC20036-2804,USA.(2)Gherna,R.,Pienta,P.,&Cote,R.(1996)Catalogue of Bacteria and Phages,19th Ed.,ATCCBooks,PO Box753,Waldorf,MD20604-0753,USA.(3)U.S.Food and Drug Administration(1995)Bacteriological Analytical Manual,8th Ed.,AOACINTERNATIONAL,Gaithersburg,MD20877,USA.(4)“Standard Test Methods for EvaluatingInactivators of Antimicrobial Agents Used in Disin-fectant,Sanitizer,and Antiseptic Products(Designa-tion E1054-91)”(1998)in ASTM1998Annual Bookof Standards on Water and Environmental Technol-ogy,ASTM,100Barr Harbor Dr,WestConshohocken,PA19428-2959,USA.(5)Russell,A.D.(1999)in Principles and Practiceof Disinfection,Preservation,and Sterilization,3rdEd.,A.D.Russell,W.B.Hugo,&G.A.J.Ayliffe(Eds),Blackwell Scientific Publications,Oxford,UK,pp89–113.ISBN0632041943(6)Singer,S.(1987)Cosmetics&Toiletries,102,55–59.。
产品说明书 通过FTIR和SDS-PAGE耐用性测试
Determination of edible bird's nests by FTIR and SDS-PAGE coupled with multivariate analysisLili Guo a,b,Yajun Wu a,Mingchang Liu a,Bin Wang a,Yiqiang Ge c,d,Ying Chen a,*a Agro-product Safety Research Center,Chinese Academy of Inspection and Quarantine,Beijing,100176,People's Republic of Chinab Institute of Pharmaceutical and Food Engineering,Shanxi University of Traditional Chinese Medicine,Jinzhong,030619,People's Republic of Chinac College of Food Science and Nutritional Engineering,China Agricultural University,Beijing,100083,People's Republic of Chinad China Rural Technology Development Center,Beijing,100045,People's Republic of Chinaa r t i c l e i n f oArticle history:Received12February2017 Received in revised form5April2017Accepted6May2017 Available online10May2017Keywords:Edible bird's nest(EBN) DeterminationFTIRSDS-PAGEMultivariate analysis a b s t r a c tEdible bird's nests(EBNs)have been traditionally regarded as a kind of medicinal and healthy food in China.Nowadays,EBNs in market are graded by some conventional features presented by EBN products, such as material purity,geographical origin,production environment or color.Since different EBNs have various prices,two common types of fraud with EBN,namely substituting the EBN ingredient with cheap non-EBN materials and mislabeling of a country of origin or production environment,have occurred for a long time.To explore a feasible method for identifying EBNs,Fourier Transform Infrared Spectroscopy (FTIR)system and sodium dodecyl sulfate e polyacrylamide gel electrophoresis(SDS-PAGE)combined with multivariate analysis were employed.The results showed that FTIR could discriminate between authentic and fake EBNs since there were big differences in spectra of these samples.On the other hand, protein band data obtained by SDS-PAGE could be used for distinctly identifying geographical origin of house nests and the production environment of EBNs.It is concluded that FTIR in combination with SDS-PAGE is a promising method for comprehensive determination of EBNs.©2017Published by Elsevier Ltd.1.IntroductionEdible bird's nest(EBN)is produced by the swiftlet(Aerodramus and Collocalia)which mainly inhabits in Southeast Asian countries (Indonesia,Malaysia,Philippine,Thailand,etc.).It is a precious food material for a long history in local culture(Kang,Hails,& Sigurdsson,1991;Lau&Melville,1994).In recent years,EBN mar-ket has been expanded to worldwide(Lau&Melville,1994).The major nutrition components of EBN include sialic acid,glycopro-teins,amino acids and trace mineral elements(Goh et al.,2001;Lau &Melville,1994;Marcone,2005).Medicinal functions of EBN ex-tracts have been proved by modern science(Chua,Lee,Nagandran, &Yahaya,2013;Guo et al.,2006;Hou,Xian,Liu,Lai,&Chen,2010; Kim et al.,2012;Kong et al.,1987;Vimala,Hussain,&Nazaimoon, 2012).EBNs are classified according to their geographical origin,pro-duction environment and natural color.The geographical origin refers to the country or region one EBN originates from.Indonesia is currently the largest producer for EBNs followed by Malaysia.The collection site for EBN products is called production environment here,and so far EBNs are collected from either natural caves on cliff or man-made bird houses,which simulate the natural cave envi-ronment with dark and humid conditions.Therefore there are two kinds of EBNs accoding to the production environment,namely house nests and cave nests.Since1980s,house nests have begun to dominate the EBNs market(Saengkrajang,Matan,&Matan,2013; Sankaran,2001),which makes the natural cave nests more precious.As far as the color is concerned,different EBNs present different color in natural light.Generally house nest is white and cave nest is yellow or red(Paydar et al.,2013).In market,grading of EBN products,which is implemented based on such major factors as geographical origin,production environment and color,plays a determinant role in price setting.Under normal circumstances, house nests from Indonesia charge a higher price than those from Malaysia;cave nest is more expensive than house nest;red nest asks for the highest price,followed by yellow nest and white nest (But,Jiang,&Shaw,2013;Paydar et al.,2013;Thorburn,2015).Since different EBNs have various prices,two common types of fraud with EBN have occurred for a long time to earn big profits. One is partially or totally substituting the EBN ingredient with such*Corresponding author.E-mail address:********************(Y.Chen).Contents lists available at ScienceDirect Food Controljournal ho me page:www.elsevier.co m/locate/foodcont/10.1016/j.foodcont.2017.05.0070956-7135/©2017Published by Elsevier Ltd.Food Control80(2017)259e266cheap non-EBN materials as tremella,pork skin,egg white and agar. The other is the form of mislabeling,like falsification of a country of origin claim or deliberate wrong production environment on label. Thereby authentication method is important to protect consumer confidence and maintain a fair EBN market.For the fraud type of adulteration,molecular biological methods have been reported to identify DNA from swiftlet and cheap adulterants(Guo et al.,2014) and to identify EBN specific proteinfingerprint(Wu et al.,2010). These methods are sensitive but involve careful extraction of spe-cific components.FTIR is based on the absorption spectrum of various molecular groups,reflecting the existence of chemical components by wavelength and intensity.It is good at catching the whole picture of chemical compounds and is widely used in food authentication,especially when fast screening of large amount of samples is required(Rodriguez-Saona&Allendorf,2011;de la Mata et al.,2012).As for the fraud type of mislabeling,amino acid composition analysis combined with chemometrics was applied to differentiation between house and cave EBNs(Seow,Ibrahim, Muhammad,Lee,&Cheng,2016).However,research on determi-nation of geographical origin of EBNs was scarcely reported.A number of techniques,such as isotope ratio mass spectrometry, inductively coupled plasma mass spectrometry,infrared spectros-copy and chromatography have been utilized in thisfield(Luykx& van Ruth,2008).Among them,the isotopic analysis is an important strategy for accurately verifying geographical origin of numerous food products(Laursen,Schjoerring,Kelly,&Husted,2014;Reid, O'Donnell,&Downey,2006;Zhao et al.,2014).However,it is quite expensive due to the high cost of its sample preparation and the high price of equipment.SDS-PAGE has been proved to be an effective method for identifying geographical origins of traditional Chinese medicine(Bai,Zhao,Zhang,Wang,&Li,2012;Wang,Gao, &Feng,2012;Zhang,2011).Based on the above,this study aimed to explore the potential application of FTIR and SDS-PAGE coupled with multivariate analysis on comprehensive EBN authentication, especially on identification EBN samples from different areas and different production environments.2.Materials and methods2.1.SamplesSeventeen raw materials of EBN(as shown in Fig.1S in Sup-plementary material)were collected from Malaysia and Indonesia with the help of Edible Bird's Nest Market Committee of China Agricultural Wholesale Markets Association(EBMC).Twenty-six commercial EBN samples were supplied by Guangdong Entry-Exit Inspection&Quarantine Bureau of China.Detailed information of all EBN samples was listed in Table1.Dried white fungus,fried pigskin and eggs were purchased from a local market.Agar powder made from Gelidium amansii was purchased from Beijing Land Bridge Technology Co.,Ltd.2.2.FTIR spectra acquisition and data analysisThe FTIR spectrometer of Frontier FT-IR/NIR(Perkin Elmer, Norwalk,CT,USA)equipped with a middle infrared(MIR)triglycine sulfate(TGS)detector and the software of PerkinElmer Spectrum (Version10.00.00)was used to measure the spectra.The tremella fruit body,fried pigskin,agar powder and EBN samples were ground thoroughly using a TissueLyser II(Qiagen,Hilden,Germany) system and mixed well with potassium bromide(KBr),then were directly compressed into thinflakes.Egg white wasfirstly lyophi-lized in Modulyod-230Freeze Dryer(Thermo Scientific,Waltham, MA,USA)and then processed as above.Samples were scanned from 4000to650cmÀ1for32scans with a resolution of4cmÀ1at room temperature.Triplicate measurements for each sample were per-formed and FTIR spectra were displayed as absorbance values.MATLAB software(The MathWorks,Inc.,Natick,MA,USA)was used for data processing.The original spectrum was smoothed with a seven-point Savitzky-Golay smooth function to remove the noise. Meanwhile,standard normal variate(SNV)transformation was performed to eliminate the scattering caused by sample particles. After baseline correction,the spectrum data in the region 1800e800cmÀ1was selected for Principle Component Analysis (PCA).2.3.Preparation of water-soluble proteinThe water-soluble protein extraction from EBN samples was performed byfirst removing the feather in EBNs with tweezers,and then grinding the EBN samples intofine powder in liquid nitrogen with TissueLyser II.For each ground sample,a weight of500mg was put in a50mL centrifuge tube,followed by adding a quarter piece of Protease Inhibitor Cocktail Tablet(EDTA-free,Roche, Mannheim,Germany)and10mL ultrapure water into the tube. After complete dissolution of the tablet,the sample was incubated at60 C while shaking at750rpm for3h in a Thermomixer Comfort (Eppendorf,Hamburg,Germany).The supernatant was collected by centrifuging the solution at12,000Âg for10min and was distributed into six clean microtubes(1.5mL)with1.0mL for each tube.Afterward,the tube was centrifuged in a vacuum concentrator (Concentrator Plus5305,Eppendorf,Hamburg,Germany)until the liquid was reduced to100m L.The concentrated solution was pu-rified by ReadyPrep2-D Cleanup Kit(Bio-rad,Hercules,CA,USA) following the instruction.The protein in pellet form wasfinally obtained and stored atÀ20 C for use.2.4.SDS-PAGEDried protein sediment of EBNs was dissolved in100m L of double distilled water at4 C for1h,followed by centrifuging at12, 000Âg for5min.The supernatant was quantified by BCA Protein Assay Kit(Novagen,Madison,WI,USA)according to the instruction manual.SDS-PAGE was performed according to the following protocol described by Simpson(2003).Briefly,protein solution with protein amount approximately15m g was mixed with an equal volume of2ÂSDS-PAGE loading buffer.The mixture was then heated at95 C for5min.The denatured proteins were separated on10%SDS e PAGE mini gels at120V/20mA at4 C for approxi-mately2.5h in1ÂSDS running buffer(25mM Tris base,192mM glycine,0.1%(w/v)SDS).After the electrophoresis,gels werefixed with self-prepared stationary liquid(50%methanol,10%acetic acid and40%water)for0.5h and stained with Bio-safe Coomassie G-250Stain(Bio-rad,Hercules,CA,USA)for1h.The gels were then destained overnight in ultrapure water.To determine the molecular weight,5m L of Prestained SDS-PAGE Standards(Broad Range,Bio-rad,Hercules,CA,USA)was applied.Gels images were captured by Pharos FX Molecular Imager(Bio-rad,Hercules,CA,USA).Each sample was separated for three times with the same procedure.2.5.SDS-PAGE analysisThe captured gel images were analyzed by Quantity One soft-ware(Bio-rad,Hercules,CA,USA)according to the user guide. Molecular weight of each band was auto-calculated according to protein marker.Quantitative analysis of protein gels was conducted by using Quantity One software to obtain the absolute band density (INT/mm2)of each protein band.For the sake of eliminating the discrepancy caused by possible difference in protein loading amount,normalization of the absolute band density was performedL.Guo et al./Food Control80(2017)259e266 260。
BeatBuddy Mini 2 快速启动指南说明书
The BeatBuddy Mini 2 sounds best when played through a full-range neutral sound system.Many guitar and bass amps are either not full-range (they muffle the higher frequencies) and/or provide distortion, which lowers the BeatBuddy Mini 2's sound quality.Acoustic guitar amplifiers, PA systems, and home stereos tend to be neutral & full-range.Sound Quality1The BeatBuddy Mini 2 does not change your instrument's sound.The input jack lets you plug all of your pedals into the same sound system, no mixer necessary.The volume knob only controls the BeatBuddy Mini 2's drums. It does not affect the volume of any instrument plugged into it.If you are plugging in other pedals, the BeatBuddy Mini 2 should be last on the pedal effects chain, after the looper,so that its sound isn’t affected by your other pedals.You do NOT need to have an instrument plugged in for the BeatBuddy Mini 2 to produce sound.2A light indicates whether you are selecting Genre, Song, or Tempo, all of which you control with a single knob.1. Choose one of BeatBuddy Mini 2's 24 genres by rotating the knob. Select by pressing down on the knob like a button. The green light advances from “Genre” to “Song”.Selecting a Song32. Twist knob to scroll through songs within a genre. Each song’s time signature and default tempo are displayed on the bottom row. Changing Tempo: To change the tempo,press the knob again. The light advancesfrom “Song” to “Tempo”. Adjust the tempo by rotating the knob.4Controlling the Beat Start: Begin song by tapping the pedal. The song starts with an intro* fill, just like a real drummer, and then goes to the verse, which loops as long as you'd like. Fills: If you want a fill, just tap the pedal any time, and a fill will play, perfectly in time.Transition: To transition to the chorus, hold the pedal down for as long as you’d like the transition beat to last. Chorus: When you’re ready to start the chorus, let go. The chorus beat loops as long as you like. Return to verse the same way you transitioned to chorus.End: Whenever you want to end the song, double-tap the pedal to endwith an outro fill.5Single tapHold downDouble tap6See The Beat7for our song-matching tools.Not sure which beat to use for your favorite songs?8With the Singular Sound Footswitch, select genres & songs, change tempo, add accent hits and drum breaks anytime — creating a powerful, realistic live effect, all totally hands-free.Sold separately at your favorite dealer and at /products Additional Control!Only the Singular Sound Footswitch is compatible with the BeatBuddy Mini 2While no song is playing:Hold down to select Genre , Song or Tempo Genre : Tap once to move to next genre Song : Tap once to move to next song Tempo : Tap tempo modeWhile song is playing: Pause/Unpause Accent hit (e.g. a cymbal crash)Accent hits vary by song and sometimes even by song part9Need Even More?The original, critically-acclaimed BeatBuddy offers even more power, with:• Professional level sound quality• Full color LCD screen• MIDI Sync• Drum set selection• Infinite customization• Unlimited additional content Learn more at 10To be covered by our warranty, please register your BeatBuddy Mini 2 now (you'll forget later, we promise):/warrantyYour BeatBuddy Mini 2 comes with a 2 yearlimited warranty on parts and workmanshipfrom the date of purchase. During thisperiod, we will repair or replace (at our option) defective units free of charge.The warranty remains valid only if the serialnumber on the unit is not defaced or removed.It does not cover damage due to misuse,unauthorized tampering, accident or neglect.11。
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a r X i v :h e p -e x /9906003v 1 1 J u n 1999MiniBooNE:the Booster Neutrino ExperimentAndrew O.BazarkoPrinceton University,Princeton,NJ 08544∗The Booster Neutrino Experiment at Fermilab is preparing to search for νµ→νe oscillations.Theexperiment is designed to make a conclusive statement about LSND’s neutrino oscillation evidence.The experimental prospects are outlined in light of the current results from LSND and KARMEN.I.INTRODUCTIONCurrently,the Liquid Scintillator Neutrino Detector (LSND)at the Los Alamos National Laboratory is the only accelerator-based neutrino experiment to have evidence for neutrino oscillations [1].The Booster Neutrino Experiment (BooNE)at Fermilab is being prepared to conclusively test these results.The experiment will take place at a new neutrino beamline coming offof the FNAL 8GeV proton Booster.The first phase of BooNE —MiniBooNE —will be a single-detector experiment.MiniBooNE will obtain approximately 1000events per year if the LSND signal is due to νµ→νe oscillations,and will be capable of establishing the signal with greater than 5σsignificance.This new experiment expects to be collecting data by the end of 2001.KARMEN,the Karlsruhe Rutherford Medium Energy Neutrino experiment at the Rutherford Appleton Laboratory,is very similar to LSND,has been running since 1990,and will be completed in early 2001.Therefore we discuss the results of both KARMEN and LSND to highlight the experimental context that MiniBooNE will encounter.II.LSND AND KARMEN LSND presented its first evidence for ¯νµ→¯νe oscillations in 1995[2].In the same year,KARMEN completed its first phase of searching for the same oscillations,but lacked the sensitivity to confirm or refute LSND [3].After upgrading its cosmic ray veto [4],KARMEN resumed data taking in February 1997and plans to continue running through 2001.Results based on KARMEN data collected up to April 1998were available at the time of DPF’99[5].More recently KARMEN has updated results based on data collected through February 1999[6].LSND and KARMEN both search for ¯νµ→¯νe .Both experiments are at 800MeV proton accelerators where muon-antineutrinos are produced from the decay of muons at rest,through the decay chain:π+→µ+νµ֒→e +νe ¯νµendpoint 52.8MeVBoth experiments employ liquid scintillator detectors.Without being able to directly distinguish e +from e −,the experiments distinguish the appearance of ¯νe from the presence of νe by correlating the electron-type track in position and time with the photon from an associated neutron capture reaction.Neutrons captured on protons produce 2.2MeV photons:¯νe p →e +nn p →d γ(2.2MeV)There are,of course,differences between the two experiments [7].The LSND detector is three times the size of the one at KARMEN,167tons versus 56tons;and LSND’s proton exposure of more than 25000C outpaces KARMEN’spost-upgrade expectation ofm forKARMEN.By being furtherflux.LSND is a single tankin LSND is lower than at LSND can measure the track at LSND includes a drift space charge conjugateνµ→νe oscillationFIG.1.KARMEN90%LSND favored region and limits fromBased on analysis of data0candidate events while to an LSND-type signal is on the order of1event.Figure1shows the limit derived from this experimental observation overlaying the LSND favored region.Also shown is KARMEN’s sensitivity,the limit if only the background expectation were observed.Noting that KARMEN expects three times more data,a glance at Figure1raises the question,At this rate won’t KARMEN soon rule out LSND?Well,not so fast.The difference between KARMEN’s sensitivity and limit curves merits scrutiny.The limit here benefits from the non-observation of even the expected background.KARMEN will keep running,and assuming that events are seen in the future,which would be more consistent with the background expectation,KARMEN’s limit will necessarily move closer to its sensitivity contour.The sensitivity will improve with more data and with a more sophisticated likelihood analysis.Nevertheless,KARMEN will lack the sensitivity to rule out or confirm LSND.The situation is illustrated by KARMEN’s recently updated results,based on data gathered through February1999, about half of its ultimate total.With a new analysis,8events are observed,while7.8±0.5background events are expected.For the favored LSND parameters,KARMEN would expect between1.5and5.5oscillation events.The limit is now weaker than in Figure1.These results encroach on LSND’s allowed region,but they do not rule out LSND.Another experiment will be needed to make a conclusive statement about LSND,and that is where MiniBooNE comes in.III.MINIBOONE:EXPERIMENTAL DESIGNMiniBooNE will use a new neutrino beamline coming offof the FNAL8GeV proton Booster.The Booster is a reliable machine,expected to provide2×107s of running per year,while delivering5×1012protons per1µs pulse at a rate of5Hz to MiniBooNE.Furthermore,the Booster will be able to deliver beam to MiniBooNE while also supplying protons for the TeVatron and NuMI programs.The secondary pion beam will emerge from a two-horn focusing system into a50m decay region.The pion decay length will be either25m or50m depending on the position of a movable steel beam stop(varying the decay length provides a check of experimental systematics).The detector will be positioned500m downstream of the decay region. The detector will consist of a spherical tank6.1m(20feet)in radiusfilled with807tons of pure mineral oil.An inner-tank structure at5.75m will support phototubes and form an optical barrier,separating the tank into a central main volume and an outer veto shield.Cherenkov and scintillation light from neutrino interactions in the main volume will be detected by12808-inch phototubes,providing10%photocathode coverage of the445tonfiducial volume. (Undoped mineral oil tends to scintillate modestly from the presence of intrinsic impurities.)The veto shield will be viewed by240phototubes mounted on the tank wall.Typical neutrino energies will be from0.5to1.0GeV.In one year of running,the experiment will collect approx-imately500000reconstructedνµevents.The intrinsicνe contamination in the beam will be approximately0.3%or approximately1500reconstructedνe background events.IV.MINIBOONE:ANALYSIS DESCRIPTIONThe detector will reconstruct quasielasticνe interactions by identifying electrons via their characteristic Cherenkov and scintillation light signatures.Besides theνe→e−signal,several backgrounds will contribute.The analysis will come down to accounting for the backgrounds and determining whether or not there is an excess.The background sources will be due toνe contamination in the beam and the misidentification of muons andπ0’s in the tank as electrons.Because the neutrinos are at higher energies than at LSND and KARMEN,neutrons will not play a role in the signal and will not contribute background.The detector will record the time of the initial hit and total charge for each phototube.From this information, the track position and direction will be determined.Muon tracks will be distinguished from electron tracks by their Cherenkov rings and scintillation light.Electrons will tend to produce“fuzzy”rings due to multiple scattering and bremsstrahlung,while muon rings will tend to have sharp outer boundaries.Electrons also tend to have a high fraction of prompt(Cherenkov)light compared to late(scintillation)light,whereas muons produce relatively more late light. Theνe contamination in the beam is due to decays of pions and kaons.Monte Carlo simulation constrained by production data will be used to limit the systematic uncertainty in theνe background to better than10%.In addition, it will be possible to measure the pion energy spectrum using the the observedνµevents,virtually all(99%)of which will come from pion decay.The technique exploits the classic energy-angle correlation in neutrino beams,which will be enhanced here by the relatively low beam energy and small solid angle subtended by the detector.By measuring the pion spectrum,MiniBooNE expects to reduce the uncertainty in the pion component of theνe background to less than5%.Ninety two percent of the muons contained in the detector will decay,and they will be relatively easily identified by the presence of a second track.However,the8%of muons that get captured have a greater chance to be misidentified. The misidentification of muon captures will be estimated by studying the large sample of muons that decay and determining the particle identification algorithm performance while ignoring the decay ing this technique, which does not rely on Monte Carlo simulation,the muon misidentification uncertainty is expected to be below5%. Most neutral pions will be identified by their two electromagnetic decay tracks.The small fraction(1%)of asym-metricπ0decays will not yield two resolvable tracks and will therefore be more likely to be misidentified.Themisidentification contribution of which will be constrained by the large sample of measured is expected to be5%.If oscillations occur as1000events in one year of running.Figure2shows two points in the LSND favored region.The significance is background sources above.FIG.2.The expected number of points in the LSND favored region.MiniBooNE will gain of theνe events.The oscillation signal has a different energy of the background will not necessarily lead to a for the energy-dependent fit as well as the limit based onVI.CONCLUSIONSLSND has presented evidence for muon to electron neutrino oscillations.KARMEN is now searching for these oscillations,but KARMEN is unlikely to have the sensitivity to reach a conclusion about LSND.Another experiment will be needed.MiniBooNE is being prepared tofill this need.The detector and new8GeV beam line are being designed at Fermilab,and the experiment is scheduled to start data taking at the end of2001.MiniBooNE will either rule out LSND or it will demonstrate the signal and home in on the parameters.Should a single be found,BooNE would be ready to continue its experimental program with a second detector,the position of which determined by the MiniBooNE result.VII.ACKNOWLEDGEMENTSIt is a pleasure to thank my MiniBooNE colleagues.I thank Eric Church for discussions about LSND and Klaus Eitel for discussions about KARMEN.。