分子生物学实验-Molecular Biology Practical
合集下载
相关主题
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
4.Correctly usethe related experimental instruments such asmicro-sample feeder, high pressure steam sterilizer, constant temperature shaking table, super clean workbench, high speed refrigerated centrifuge, ice maker, PCR instrument, horizontal electrophoresis apparatus, gel imaging systemetc.beskilled in aseptic operation technologyand developed withindependent operation ability and teamwork cooperation spirit.
2.Master the process and basic steps of the experimental technology of bacterial plasmid DNA artificial recombination, as well asthe main experimental techniques of the DNA artificial recombination of animal and plant cells,design the experimentwiththese techniques,observe and analyze the experimental phenomena.
Learning Schedule
• Lab 1 Brief introduction, Genomic DNA extraction; PCR amplification
• Lab 2 Construction of recombinant DNA, transformation and selection
• Lab 3 plasmid DNA extraction and Qualitative and Quantitative Analysis
• Lab 4 Total RNA extraction, Reverse transcription and RT-PCR
Pre-requisites
Molecular Biology
Intended Learning Outcomes
The anticipated knowledge, skills and attitude to be developed by the studentsare:
1.Understand the technical system and development status of molecular biology experiment,toenhancethe subjective desire to deeply understand the theoretical knowledge and technical principles of molecular biology, more activelytoexplorerelevant scientific problems in the field of molecular biology and to solve practical problemsusing existing technologies.
3.Independentlyconductgenomic DNA extraction, Plasmid Extraction, total RNA extraction, PCR amplification, RT-PCR amplification, agarose gel electrophoresis;observe and analyze the experimental phenomena;Independentlyconductthe experimental operation of transformingtarget genesinto the competentE.colicellsviaplasmid DNA.
Syllabus ofBiotechnologyatBaidu NhomakorabeaHaide College
Molecular Biology Practical
Description
Molecular Biology Laboratory is a system of molecular biology experiment technology based on biochemistry experiment and microbiology experiment, which is a modern biological technology for research and application of life science. This course is aimed at senior students majoring in biology and biotechnology. It mainly introduces the basic operational techniques and experimental means of molecular biology, including Principles and procedures of experimental projects such as genomic DNA extraction and identification, plasmid DNA extraction and identification, total RNA extraction and identification, PCR amplification, restriction endonuclease digestion, processofmolecular cloning and construction of gene library. Through the course study and operation practice, students are required to master the basic principles and operation skills of molecular biology experiments, and to be able to analyze and solve practical problems in related fields of molecular biologywiththese techniques.
2.Master the process and basic steps of the experimental technology of bacterial plasmid DNA artificial recombination, as well asthe main experimental techniques of the DNA artificial recombination of animal and plant cells,design the experimentwiththese techniques,observe and analyze the experimental phenomena.
Learning Schedule
• Lab 1 Brief introduction, Genomic DNA extraction; PCR amplification
• Lab 2 Construction of recombinant DNA, transformation and selection
• Lab 3 plasmid DNA extraction and Qualitative and Quantitative Analysis
• Lab 4 Total RNA extraction, Reverse transcription and RT-PCR
Pre-requisites
Molecular Biology
Intended Learning Outcomes
The anticipated knowledge, skills and attitude to be developed by the studentsare:
1.Understand the technical system and development status of molecular biology experiment,toenhancethe subjective desire to deeply understand the theoretical knowledge and technical principles of molecular biology, more activelytoexplorerelevant scientific problems in the field of molecular biology and to solve practical problemsusing existing technologies.
3.Independentlyconductgenomic DNA extraction, Plasmid Extraction, total RNA extraction, PCR amplification, RT-PCR amplification, agarose gel electrophoresis;observe and analyze the experimental phenomena;Independentlyconductthe experimental operation of transformingtarget genesinto the competentE.colicellsviaplasmid DNA.
Syllabus ofBiotechnologyatBaidu NhomakorabeaHaide College
Molecular Biology Practical
Description
Molecular Biology Laboratory is a system of molecular biology experiment technology based on biochemistry experiment and microbiology experiment, which is a modern biological technology for research and application of life science. This course is aimed at senior students majoring in biology and biotechnology. It mainly introduces the basic operational techniques and experimental means of molecular biology, including Principles and procedures of experimental projects such as genomic DNA extraction and identification, plasmid DNA extraction and identification, total RNA extraction and identification, PCR amplification, restriction endonuclease digestion, processofmolecular cloning and construction of gene library. Through the course study and operation practice, students are required to master the basic principles and operation skills of molecular biology experiments, and to be able to analyze and solve practical problems in related fields of molecular biologywiththese techniques.