TTP 22_329907-28-0_DataSheet_MedChemExpress
碧云天生物技术 Beyotime Biotechnology 生物素标记EMSA探针说明书
碧云天生物技术/Beyotime Biotechnology订货热线：400-168-3301或800-8283301订货e-mail：******************技术咨询：*****************网址：碧云天网站微信公众号生物素标记EMSA探针－β-Catenin/TCF (0.2μM)产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl产品简介：生物素标记EMSA探针－β-Catenin/TCF是用于EMSA(也称gel shift)研究的生物素(Biotin)标记的β-Catenin/TCF consensus oligonucleotide。
这个生物素标记的双链寡核苷酸含有公认的β-Catenin/TCF结合位点，可以用作EMSA研究时的探针。
β-Catenin/TCF consensus oligo的序列如下：5'-CCC TTT GAT CTT ACC-3'3'-GGG AAA CTA GAA TGG-5'本生物素标记EMSA探针已经过纯化，可以直接用于EMSA结合反应。
本生物素标记EMSA探针可以和碧云天的化学发光法EMSA试剂盒(GS009)配套使用。
一个包装的生物素标记探针可以进行约200-400个样品的EMSA检测。
包装清单：产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl—说明书1份保存条件：-20ºC保存，一年有效。
注意事项：避免加热到40ºC以上，温度过高会导致双链DNA探针解聚成单链。
而单链无法用于EMSA研究。
对于基于生物素标记的EMSA检测的详细操作可以参考碧云天的化学发光法EMSA试剂盒(GS009)的使用说明。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
沃伯-福奇 KFD2-SOT2-Ex2 区间2分区2区域2分区1分区2区域2分区1分区10k
17-08-09 14:30D a t e o f i s s u e 2017-08-09181005_e n g .x m l24 V DC14+15-78 (+/-)9III2+3-1+5+6-4+ConnectionAssembly•2-channel isolated barrier •24 V DC supply (Power Rail)•Dry contact or NAMUR inputs•Passive transistor output, non-polarized •Line fault detection (LFD)•Reversible mode of operation •Up to SIL 2 acc.to IEC 61508FunctionThis isolated barrier is used for intrinsic safety applications. It transfers digital signals (NAMUR sensors/mechanical contacts) from a hazardous area to a safe area.Each proximity sensor or switch controls a passive transistor output for the safe area load. The normal output state can be reversed using switch S1 for channel I and switch S2 forchannel II. Switch S3 enables or disables line fault detection of the field circuit.During an error condition, the transistors revert to their de-energized state and LEDs indicate the fault according to NAMUR NE44.A unique collective error messaging feature is available when used with the Power Rail system.2Features17-08-09 14:30D a t e o f i s s u e 2017-08-09181005_e n g .x mlGeneral specifications Signal typeDigital Input Functional safety related parameters Safety Integrity Level (SIL) SIL 2Supply Connection Power Rail or terminals 14+, 15-Rated voltage U r 20 ... 30 V DC Ripple ≤ 10 %Rated current I r≤ 50 mA InputConnection side field sideConnection terminals 1+, 2+, 3-; 4+, 5+, 6-Rated valuesacc. to EN 60947-5-6 (NAMUR), see system description for electrical data Open circuit voltage/short-circuit current approx. 8 V DC / approx. 8 mA Switching point/switching hysteresis 1.2 ... 2.1 mA / approx. 0.2 mALine fault detection breakage I ≤ 0.1 mA , short-circuit I > 6 mA OutputConnection side control sideConnection output I: terminals 7, 8 ; output II: terminals 8, 9Switching voltage ≤ 30 VSwitching current ≤ 100 mA , short-circuit protectedSignal level 1-signal: switching voltage - 2.5 V max. at 10 mA switching current or 3 V max. at 100 mA switching current 0-signal: switched off (off-state current ≤ 10 µA)Output I, IIsignal ; electronic output, passive Collective error message Power Rail Transfer characteristics Switching frequency ≤ 5 kHzGalvanic isolation Input/Output reinforced insulation acc. to IEC 62103, rated insulation voltage 300 V rmsInput/power supply reinforced insulation acc. to IEC 62103, rated insulation voltage 300 V rms Output/power supply basic insulation according to IEC 62103, rated insulation voltage 50 V effInput/inputnot availableOutput/Output not availableIndicators/settings Display elements LEDs Control elements DIP-switchConfiguration via DIP switchesLabelingspace for labeling at the front Directive conformity Electromagnetic compatibilityDirective 2014/30/EU EN 61326-1:2013 (industrial locations)Conformity Galvanic isolationIEC 62103:2003Electromagnetic compatibility NE 21:2004Degree of protection IEC 60529:2001InputEN 60947-5-6:2000Ambient conditionsAmbient temperature -20 ... 60 °C (-4 ... 140 °F)Mechanical specifications Degree of protection IP20Connection screw terminalsMass approx. 150 gDimensions 20 x 119 x 115 mm (0.8 x 4.7 x 4.5 inch) , housing type B2Mountingon 35 mm DIN mounting rail acc. to EN 60715:2001Data for application in connection with hazardous areasEU-Type Examination Certificate PTB 00 ATEX 2035Marking ¬ II (1) G [Ex ia] IIC¬ II (1) D [Ex ia] IIIC InputEx ia IIC, Ex ia IIIC Voltage U o 10.5 V Current I o 13 mA17-08-09 14:30D a t e o f i s s u e 2017-08-09181005_e n g .x mlOutputMaximum safe voltage U m40 V DC (Attention! The rated voltage can be lower.)EU-Type Examination CertificateDMT 01 ATEX E 133Marking ¬ I (M1) [Ex ia] I Certificate TÜV 99 ATEX 1499 X Marking ¬ II 3G Ex nA II T4Galvanic isolationInput/Output safe electrical isolation acc. to IEC/EN 60079-11, voltage peak value 375 V Input/power supply safe electrical isolation acc. to IEC/EN 60079-11, voltage peak value 375 V Directive conformityDirective 2014/34/EU EN 60079-0:2012+A11:2013 , EN 60079-11:2012 , EN 60079-15:2010 , EN 50303:2000International approvals FM approval Control drawing 116-0035CSA approval Control drawing 116-0047IECEx approval IECEx PTB 05.0011Approved for [Ex ia] IIC , [Ex ia] I , [Ex ia] IIICGeneral information Supplementary information Observe the certificates, declarations of conformity, instruction manuals, and manuals where applicable. For information see .17-08-09 14:30D a t e o f i s s u e 2017-08-09181005_e n g .x mlPower feed module KFD2-EB2The power feed module is used to supply the devices with 24 V DC via the Power Rail. The fuse-protected power feed module can supply up to 150individual devices depending on the power consumption of the devices. Collective error messages received from the Power Rail activate a galvanically-isolated mechanical contact.Power Rail UPR-03The Power Rail UPR-03 is a complete unit consisting of the electrical insert and an aluminium profile rail 35mm x 15mm. To make electrical contact, the devices are simply engaged.Profile Rail K-DUCT with Power RailThe profile rail K-DUCT is an aluminum profile rail with Power Rail insert and two integral cable ducts for system and field cables. Due to this assembly no additional cable guides are necessary.Power Rail and Profile Rail must not be fed via the device terminals of the individual devices!ConfigurationAccessories。
《中国药典》(2020年版)复方磺胺恶唑片中的甲氧苄啶含量测
《中国药典》(2020年版)复方磺胺噁唑片中的甲氧苄啶含量测甲氧苄啶（Trimethoprim, TMP），又称为甲氧苄氨嘧啶、甲氧苄嘧啶，是一种抗菌增效药，与磺胺类药物联合使用时，能使磺胺类药物抗菌谱扩大、抗菌活性大大增强。
由于其独特的作用，甲氧苄啶在养殖业病害防治中被广泛应用。
迪信泰检测平台采用高效液相色谱(HPLC)和液相色谱-三重四极杆质谱(LC-MS/MS)法，可高效、精准的检测甲氧苄啶的含量变化。
此外，我们还提供其他抗生素检测服务，以满足您的不同需求。
HPLC和LC-MS测定甲氧苄啶样本要求：1. 请确保样本量大于0.2g或者0.2mL。
周期：2~3周项目结束后迪信泰检测平台将会提供详细中英文双语技术报告，报告包括：1. 实验步骤（中英文）2. 相关质谱参数（中英文）3. 质谱图片4. 原始数据5. 甲氧苄啶含量信息应用范围：本方法采用高效液相色谱法测定复方磺胺甲恶唑片中磺胺甲恶唑和甲氧苄啶的含量。
本方法适用于复方磺胺甲恶唑片。
方法原理：供试品加甲醇稀释，最终用流动相定量稀释后，进入高效液相色谱仪进行色谱分离，用紫外吸收检测器，于波长240nm处检测磺胺甲恶唑和甲氧苄啶的吸收值，计算出其含量。
试剂： 1. 0.1mol/L盐酸2. 乙腈3. 三乙胺4. 氢氧化钠试液5. 冰醋酸仪器设备： 1. 仪器1.1 高效液相色谱仪1.2 色谱柱十八烷基硅烷键合硅胶为填充剂，理论塔板数按磺胺甲恶唑峰计算不低于4000。
磺胺甲恶唑和甲氧苄啶的分离度应符合要求。
1.3 紫外吸收检测器2. 色谱条件2.1 流动相：水乙腈三乙胺=799 200 1（用氢氧化钠试液或冰醋酸调节pH 值至5.9。
）2.2 检测波长：240nm2.3 柱温：室温试样制备：1. 称取供试品取本品10片，精密称定，研细，精密称取适量（约相当于磺胺甲恶唑44mg）置100mL量瓶中。
2. 对照品溶液的制备精密称取磺胺甲恶唑对照品和甲氧苄啶对照品适量，用0.1mol/L盐酸溶液溶解并定量稀释制成每1mL中约含有磺胺甲恶唑0.44mg的和甲氧苄啶89µg的溶液。
抗体结构分析研究-赛默飞
离子聚焦八级杆
S-lens 离子源 Orbitrap 质量分析器
19
Q Exactive
HCD碰撞池 C形槽 四级杆 质量分析器
提高离子信噪比和选择性； 提高离子信噪比和选择性； 提高选择性离子扫描模式 SIM的检测限、 的检测限、定量限； 定量限； 可做数据关联的MS/MS
Orbitrap 质量分析器
5
生物质谱技术
• 特点：通用分析技术，应用范围广、灵敏度高（amol级）、特异性强、分析速度快 • 原理：使有机化合物形成离子，按其质荷比（m/z）的不同进行分离和检测 • 作用：提供不同化合物的分子量及响应强度信息，并据此进行定性和定量分析
真空系统
进样系统 (GC、LC或 大气压 者直接进样)
离子化系统
100 90 80 Relative Abundance 70 60 50 40 30 20 10 0 100 110.07122 R=128104 C5 H8 N3 -0.48552 ppm 110 120 130
Caffeine 0.98 ppm
Caffeine fragment
138.06647 R=116804 140 152.30849 R=70504 150 165.07191 R=90904 160 170
动态范围宽
4-5 magnitude
15
Orbitrap家族
落地式的LTQ Orbitrap系列 台式的Exactive系列
16
目前在售的三款LTQ Orbitrap类型
LTQ Orbitrap XL
MSn 高灵敏度 ETD, CID, PQD
离子阱部分： 将单阱 单阱升级为 单阱 双压线性离子阱
7
内容概览
新型肿瘤标志物(PIVKAII)临床应用进展及评价
healthy liver
Chronic hepatitis
Cirrhosis
慢性炎症以及逐渐加深的肝脏损伤
Cancer (HCC)
Viral marker
Fibrosis marker
Tumor marker
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现有肝癌相关肿瘤标志物
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后又随之增加。血清甲胎蛋白的含量与异常凝血酶原水平基本不具相关性。
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Research design:
Song et al. (2002)
Tsai et al. (1997)
Tsai et al. (2003)
Hsia et al. (2007)
Hsia et al. (2007)
Cui et al. (2003)
Yoon et al. (2004)
Cutoff value 2.0 ng/ml
Sensitivity 51%
• Tumor-Derived Autoantibody (TAA)
Etc.
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Markers GPC3 GP73
TGF-β1
IGF-II IL-6 IL-10 GGT HCCR
References
Hippo et al. (2004)
Marrero et al. (2005)
放射免疫诊断试剂盒
20-1000 (pg/ml)
非平衡法 24h+24h
50 100
490 700
RP-004
降钙素基因相关肽 (CGRP)
20-1200 (pg/ml)
非平衡法 48h+24h
50 100
490 700
RP-005 RJ-005 (原装进口) 心钠素 (ANP)
50-1600 (pg/ml) 0.0625-2 (ng/ml)
肾性高血压， 原发性 醛固酮增多症
25 尾加压素 UII (UII) 皮质醇结合球蛋白 (CBG) 皮质类固醇结合球蛋 白(VP)
37℃/45m
200
48/RT 2h/RT +35m
100
7200 糖尿病及 Cushing 综 合症，Add-isuan 病 及 Sheehan 综合症 糖尿病及 Cushing 综 合症，Add-isuan 病 及 Sheehan 综合症
1000 1500
RP-050
P 物质 (S.P)
5-1215 (pg/ml)
非平衡法 24h+24h
50 100
1000 1500
RP-051
血管活性肠肽 (VIP)
40-5120 (pg/ml)
非平衡法 24h+24h
50 100
1000 1500
RP-052
生长抑素 (SS)
40-5120 (pg/ml)
肌钙蛋白 (C-TnT) 脂肪酸结合蛋白 (FABP) 血管紧张素Ⅰ （肾素活性） (AⅠ/PRA) 肾素活性 （Renin） 血管紧张素Ⅱ (AⅡ)
RK-016 RJ-016 (原装进口) RK-017
高血压分型， 治疗用 药指导
顶空气相色谱法测定空心胶囊中环氧乙烷残留量
顶空气相色谱法测定空心胶囊中环氧乙烷残留量赵振午;孟颖;孙瑞贞;马丽;胡永钢;张生万【摘要】针对2010年版中国药典规定的顶空气相色谱法测定空心胶囊中环氧乙烷残留量的干扰物问题,使用气相色谱-质谱(GC-MS)联用技术进行了系统的考察,发现干扰物主要来自实验用水中溶解的CO2,对实验用水在使用前加热煮沸即可有效地去除干扰.同时,选用RTX-WAX色谱柱,在柱温40℃,载气流量0.8 mL/min时分离效果和峰型最好.在此基础上对药典方法进行了改进,实验结果表明:环氧乙烷含量为0.21 ～12.67μg时呈良好的线性关系,相关系数r=0.999 9;最低检出限为0.03μg,加标回收率为98.6％～103.2％,相对标准偏差为0.7％～1.7％.【期刊名称】《浙江师范大学学报（自然科学版）》【年(卷),期】2013(036)002【总页数】5页(P193-197)【关键词】空心胶囊;环氧乙烷;干扰物;气相色谱-质谱联用;顶空气相色谱【作者】赵振午;孟颖;孙瑞贞;马丽;胡永钢;张生万【作者单位】山西大学生命科学学院,山西太原030006;山西大学生命科学学院,山西太原030006;山西大学化学化工学院,山西太原030006;山西大学生命科学学院,山西太原030006;山西大学化学化工学院,山西太原030006;山西大学生命科学学院,山西太原030006【正文语种】中文【中图分类】O656.31空心胶囊生产过程中大多采用环氧乙烷(EO)灭菌.环氧乙烷是一种气态广谱杀菌剂，可以杀灭各种微生物，包括细菌、繁殖体、芽孢、病毒等[1]，因其具有效果好、持效期长、操作简单等特点，目前已广泛应用于医药领域[2-4].然而，过量的环氧乙烷对人体毒害较大，不仅会引起急性中毒，还具有致过敏、致突变和致癌作用[5-7].为此，2010版中国药典规定环氧乙烷在空心胶囊中的残留量应小于0.000 1%[8].目前，文献报道的空心胶囊中环氧乙烷的测定方法主要有2类：一是张雅青等[9]利用玻璃填充柱气相色谱法测定空心胶囊中环氧乙烷残留量，该方法利用填充柱直接进样，分离效果较差，不能使杂质和环氧乙烷较好地分离；另一为2010版药典规定的顶空气相色谱法测定空心胶囊中环氧乙烷残留量的标准检测方法[8].笔者在使用药典方法测定空心胶囊中环氧乙烷残留量的过程中，发现即便样品无环氧乙烷存在时仍能检出一定量的环氧乙烷，造成空心胶囊中环氧乙烷残留量偏高.为此，使用气相色谱-质谱(GC-MS)联用技术对顶空气相色谱法测定空心胶囊中环氧乙烷残留量的干扰物进行了系统考察，发现了干扰物及其主要来源，并对色谱柱及色谱条件进行了选择和优化，在此基础上对药典方法进行了改进.该方法用于山西广生胶囊有限公司生产的空心胶囊中环氧乙烷残留量的检测，取得了满意的结果.1.1 主要仪器与试剂Agilent 7890-5975C型GC/MS联用仪(配有FID检测器),Agilent 7694顶空进样器(美国Agilent公司)，色谱柱分别为RTX-WAX(30 m×0.25 mm×0.5μm ),BP21(25 m×0.32 mm×0.25 μm)和RTX-1(30 m×0.25 mm×0.25 μm)，20 mL顶空瓶.环氧乙烷(含量≥98%,上海天莲精细化工有限公司).空心胶囊(山西广生胶囊有限公司).1.2 实验方法1.2.1 实验用水的制备将蒸馏水加热煮沸30 min后，加盖冷却,储存备用.1.2.2 样品的配制称取空心胶囊2.00 g,剪碎,加入20 mL顶空瓶中，加10.00 mL实验用水，加盖密封，摇匀待测.1.2.3 环氧乙烷溶液的配制1)环氧乙烷贮备液：取30 mL实验用水于50 mL容量瓶中，准确称量;在0 ℃以下移取环氧乙烷约0.15 mL，加入容量瓶中，不加瓶塞振摇，盖好瓶塞称量;前后2次称量之差得环氧乙烷质量为0.070 4 g，用实验用水定容至刻度，得1.41 mg/mL环氧乙烷贮备液.2)环氧乙烷标准溶液：准确移取0.75 mL环氧乙烷贮备液于50 mL容量瓶中，用实验用水定容至刻度；取此溶液5.00 mL再稀释至50 mL，得2.11 μg/mL环氧乙烷标准溶液.1.3 分析方法1.3.1 气相色谱分析条件RTX-WAX(30 m×0.25 mm×0.5 μm)色谱柱；柱温40 ℃，进样口温度250 ℃，检测器温度250 ℃；载气He，流量0.8 mL/min；分流比10∶1；顶空平衡温度80 ℃，平衡时间15 min，定量环温度90 ℃，传输线温度100 ℃；定量环体积1 mL.1.3.2 气/质联用分析条件色谱分析条件同上.质谱分析条件：电子轰击离子源(EI)；电子能量70 eV；离子源温度250 ℃；传输线温度280 ℃；四级杆温度150 ℃；质量扫描范围为18～500；数据采集速率为1.56 scans/s；色谱柱为HP-5(30 m×0.25 mm×0.25 μm)；载气He.2.1 色谱条件选择与优化2.1.1 色谱柱的选择在色谱条件下，用药典规定范围内的RTX-WAX,BP21和RTX-1三种色谱柱分别对环氧乙烷标准液进行了测定[8] ，其结果见表1.从表1可知，使用色谱柱RTX-WAX的分离效果优于色谱柱RTX-1和BP21.所以选用RTX-WAX为实验用色谱柱.2.1.2 载气流量的选择在柱温45 ℃时，用RTX-WAX色谱柱分别对载气流量为0.6，0.8，1.0，1.5，2.0 mL/min进行了考察，结果见表2.表2数据表明，环氧乙烷峰与邻近峰在载气流量0.6 mL/min时分离效果最好，但其峰形相对较差.为保证定量分析的准确度，所以选择载气流量为0.8 mL/min.2.1.3 柱温的选择在已选定的色谱条件下，对柱温为35，40，45 ℃进行了考察，结果见表3.表3数据表明，环氧乙烷峰和邻近峰的分离效果在柱温35 ℃最好，但这时环氧乙烷的峰形比40 ℃时差.所以本实验选择柱温为40 ℃.选定条件下的色谱图见图1.2.2 干扰物考察2.2.1 干扰物来源在上述选定的色谱条件下，对环氧乙烷标准溶液、未用环氧乙烷灭菌的空心胶囊及蒸馏水进行了测定，结果见图1.由图1可知，在未经环氧乙烷灭菌的空心胶囊(b)和蒸馏水(c)的色谱图中，与环氧乙烷保留时间(3.46 min)相同处仍有一峰存在，表明干扰物主要来源于实验用水(蒸馏水)，该干扰物对环氧乙烷残留量的测定会产生影响.2.2.2 干扰物表征按气/质谱联用分析条件，对蒸馏水及环氧乙烷溶液进行GC-MS分析，得到图2所示的总离子流色谱图和图3所示的质谱图.由图2可知，在气/质联用分析条件下，蒸馏水的测定仍然得到与单独色谱分析基本相同的结论，即在环氧乙烷保留时间相同处仍有干扰物质存在.对图2的空气峰和蒸馏水中干扰物峰的质谱图(见图3)进行比较，结果表明，图3(a)和图3(b)中成分相同(m/z 28为N2,m/z 32为O2,m/z 40为Ar,m/z 44为CO2)，所不同的是各离子峰相对强度不同，即各种气体的相对含量不同，尤其是图3(b)中m/z 44的CO2相对含量有所增加，表明干扰峰可能是由于蒸馏水中溶解的CO2所致.2.2.3 干扰物消除为确认上述GC-MS联用分析结果，在选定色谱条件下分别对蒸馏水、加热煮沸30 min的蒸馏水和用高锰酸钾重蒸过的蒸馏水进行了分析比较，结果如图4所示. 从图4可知，加热煮沸过的蒸馏水和高锰酸钾重蒸水，干扰峰(保留时间3.46 min)都可有效去除.这一结果进一步表明，干扰物的确来源于溶解在水中的CO2，通过加热煮沸蒸馏水即可消除干扰，并比高锰酸钾重蒸水易于制备.所以，实验用水选用加热煮沸过的蒸馏水.2.3 线性范围测定准确移取2.11 μg/mL环氧乙烷标准溶液0.10，0.50，1.00，2.00，4.00和6.00 mL于6个20 mL顶空瓶中，再分别准确量取实验用水9.90，9.50，9.00，8.00，6.00和4.00 mL，配成待测样品溶液，其中环氧乙烷含量分别为0.21，1.06，2.11，4.22，8.45和12.67 μg，平行配置3份，在选定的色谱条件下进行测定.以峰面积为纵坐标，环氧乙烷含量为横坐标，绘制标准曲线，得回归方程Y=25761X+2 108.5，r=0.999 9.结果表明，环氧乙烷含量为0.21～12.67 μg时呈良好的线性关系.以3倍噪声比计算，环氧乙烷的检出限为0.03 μg.2.4 精密度实验在6个20 mL顶空瓶中，分别加入2.32 μg/mL环氧乙烷溶液1.00 mL，再分别准确加入9.00 mL实验用水，密封、摇匀，分别进行色谱测定，结果如表4所示.从表4可知，相对标准偏差为1.1%，表明本方法精密度良好.2.5 重复性实验取同一批号(211050419)空心胶囊6份，按1.2.2方法制备样品，对环氧乙烷残留量进行色谱测定,结果如表5所示.从表5数据可知，相对标准偏差为1.5%，表明本方法重复性良好.2.6 加标回收率实验对同一批号(211050419)空心胶囊，每份准确称取2.00 g，取15份，分别置于15个20 mL顶空瓶中，其中：1,2,3,4,5号瓶各准确加入2.99 μg/mL环氧乙烷溶液1.00 mL，再加入实验用水9.00 mL；6,7,8,9,10号瓶各准确加入4.20 μg/mL 环氧乙烷溶液1.00 mL，再加入实验用水9.00 mL；11,12,13,14,15号瓶各准确加入5.75 μg/mL环氧乙烷溶液1.00 mL，再加入实验用水9.00 mL，密闭封口，摇匀，进行色谱测定，结果如表6所示.从表6可以看出，回收率为98.6%～103.2%，相对标准偏差为0.7%～1.7%，表示本方法满足检测需求.2.7 样品分析取批号210111759，211040149和211050419的3批空心胶囊产品，每批产品按1.2.2制样方法平行制备3份样品，分别用本方法(检测用水为煮沸过的蒸馏水)和药典方法(检测用水为未处理的蒸馏水)进行色谱测定，结果见表7.2010版中国药典2部明胶空心胶囊的环氧乙烷检测项[8规定：供试品溶液(2.00 g胶囊加10.00 mL水制成的顶空样品)中环氧乙烷的峰面积，不得大于对照品溶液(取2.00 μg/mL环氧乙烷溶液1.00 mL加9.00 mL水制成的顶空样品)中环氧乙烷的峰面积.从表7可知：药典方法比本方法的测定结果偏高；两种方法的结果都是批号211040149产品不合格，其余2批产品均符合药典要求.采用GC-MS发现环氧乙烷残留量测定中的干扰物主要来自实验用水中溶解的CO2，对实验用水在使用前加热煮沸，即可有效去除干扰.同时，选用RTX-WAX 色谱柱，在柱温40 ℃，载气流量0.8 mL/min的分离效果和峰形较好.在此基础上对药典方法进行了改进，该方法用于空心胶囊中环氧乙烷残留量的检测，取得了满意的结果.【相关文献】[1]杨君,王燕萍,王敏荣.顶空气相色谱法检测一次性医疗用品中环氧乙烷残留量[J].中国卫生检验杂志,2010,20(6):1345-1346.[2]Thomas R Z,Ruben J L,ten Bosch J J,et al.Effect of ethylene oxide sterilization on enamel and dentin demineralization in vitro[J].J Dent,2007,35(7):547-551.[3]Azzam M G,Roy M E,Whiteside L A.Second-generation locking mechanisms and ethylene oxide sterilization reduce tibial insert backside damage in total knee arthroplasty[J].J Arthroplasty,2011,26(4):523-530.[4]Archer E,Allen H,Hopwood A,et al.Validation of a dual cycle ethylene oxide treatment technique to remove DNA from consumables used in forensic laboratories[J].Forensic Sci Int Genet,2010,4(4):239-243.[5]张霞,周雯,王有森.环氧乙烷在灭菌物品中残留量测定及毒性研究进展[J].中国消毒学杂志,2005,22(2):217-218.[6]Natarajan A T,Preston R J,Dellarco V,et al.Ethylene oxide:evaluation of genotoxicity data and an exploratory assessment of genetic risk[J].Mutat Res,1995,330(1/2):55-70.[7]Melnick R L,Sills R parative carcinogenicity of 1,3-butadiene,isoprene,and chloroprene in rats and mice[J].Chem Biol Interact,2001,135/136:27-42.[8]国家药典委员会.中华人民共和国药典:二部[M].2010年版.北京:中国医药科技出版社,2010:1204-1205.[9]张雅青,李平.气相色谱法测定空心胶囊中环氧乙烷残留量[J].江西医药,1987,22(5):459-461.。
碧云天BeyoFast Probe qPCR Mix (2X, Low ROX)说明书
BeyoFast™ Probe qPCR Mix (2X, Low ROX)产品编号产品名称包装 D7272-1ml BeyoFast™ Probe qPCR Mix (2X, Low ROX) 1ml D7272-5mlBeyoFast™ Probe qPCR Mix (2X, Low ROX) 5ml D7272-25ml BeyoFast™ Probe qPCR Mix (2X, Low ROX)25ml产品简介：碧云天的BeyoFast™ Probe qPCR Mix (2X, Low ROX)是一种基于TaqMan等探针的用于实时荧光定量PCR，即探针法qPCR(Quantitative PCR)或real-time PCR的高品质预混液，主要用于cDNA和基因组DNA等的特异性超高灵敏度定量检测。
由于使用的探针一般是TaqMan探针，所以本方法也常称作TaqMan探针法。
本产品特别适合用于低丰度或高特异性的目的基因的定量或定性检测。
例如一些低丰度的mRNA、lncRNA、小RNA、微生物RNA反转录后的高灵敏度检测，一些相似度较高的常规染料法难以区分的同源基因或者基因的SNP检测。
检测原理：探针法qPCR不使用荧光染料如SYBR Green等对PCR产物进行荧光染色，而是采用了荧光基团和淬灭基团(quencher)标记的DNA探针靶向拟通过PCR检测的目标序列(设计的探针结合位点通常位于两条引物结合位点之间)。
正常情况下，探针上的淬灭基团由于空间上的荧光共振能力转移(FRET)而导致荧光基团淬灭。
在PCR反应扩增目标序列时，引物和探针都会退火到目标基因上，随着引物的延伸，Taq酶的5'→3'外切酶活性会导致结合在目标序列上的探针从5’端开始逐渐被降解。
探针的荧光基团和淬灭基团被Taq酶切开后，淬灭基团的作用消失，荧光基团就能正常地被激发光所激发而产生荧光了。
每经过一个PCR循环，就会有更多的荧光基团被释放，荧光强度与新合成的目标片段数量成正比，从而就可以实现定量检测了。
硝苯地平缓释片(II)CTD资料
注册分类第1 页共126 页上海玉瑞生物科技（安阳）药业有限公司硝苯平缓释片（Ⅱ）申报生产资料 模块 --3.2.P.5 制剂质量控制CTD第 2 页 共 126页表 3.2.P.5.3.2-2 鉴别（二）保留时间统计表表 3.2.P.5.3.3.3.1-2 中国药典流动相（甲醇 - 水（ 60：40））实验结果注：峰纯度合格标准：纯度角度＜纯度阈值。
结论：中国药典流动相中酸破坏样品杂质Ⅰ峰、杂质Ⅱ峰纯度不合格，且杂质Ⅰ峰与前杂质峰分离度不合格。
碱破坏样品杂质Ⅰ峰纯度不合格表 3.2.P.5.3.3.3.1-3美国药典流动相（乙腈 - 甲醇 - 水（ 50：25：25））实验结果上海玉瑞生物科技（安阳）药业有限公司硝苯平缓释片（Ⅱ）申报生产资料CTD 模块--3.2.P.5 制剂质量控制注：峰纯度合格标准：纯度角度＜纯度阈值。
结论：美国药典流动相中光照破坏样品杂质Ⅰ峰纯度不合格，酸破坏样品杂质Ⅰ峰纯度不合格，且杂质Ⅰ峰与前杂质峰分离度不合格。
碱破坏样品杂质Ⅱ峰纯度不合格。
; 表3.2.P.5.3.3.3.1-4英国药典流动相（乙腈- 甲醇- 水（9：36：55））实验结果第3 页共126页上海玉瑞生物科技（安阳）药业有限公司硝苯平缓释片（Ⅱ）申报生产资料 模块 --3.2.P.5 制剂质量控制CTD第 4 页 共 126页注：峰纯度合格标准：纯度角度＜纯度阈值。
结论：各破坏样品溶液中，主峰、杂质Ⅰ、Ⅱ与前后峰分离度均符合规定，峰纯度均符合要求，但主峰保留时间达到 时间可能过长。
45分钟, 后续可能杂质洗脱上海玉瑞生物科技（安阳）药业有限公司硝苯平缓释片（Ⅱ）申报生产资料 模块 --3.2.P.5 制剂质量控制第 5 页 共 126 页CTD① 中国药典流动相改进 总流速 :1.0ml/min 流动相：甲醇 - 水（45：55）表 3.2.P.5.3.3.3.2-2中国药典流动相改进（甲醇：水 -45 ：55）注：峰纯度合格标准：纯度角度＜纯度阈值。
Proteintech CD Marker Antibodies目录说明书
CD MARKER ANTIBODIESThe cluster of differentiation (abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules. So-called CD markers are routinely used for the immunophenotyping of cells. Despite this use, they are not limited to roles in the immune system and perform a variety of roles in cell differentiation, adhesion, migration, blood clotting, gamete fertilization, amino acid transport and apoptosis, among many others. As such, Proteintech’s mini catalog featuring its antibodies targeting CD markers is applicable to a wide range of research disciplines.IntroductionImmunohistochemical of paraffin-embedded human hepatocirrhosis using PECAM1, CD31 Figure 1: Immunofluorescence staining of PECAM1 (11256-1-AP), Alexa 488 goatImmunohistochemical staining of paraffin-embedded human colon tissue using NCAM1 Mouse brain tissue lysate was separated by SDS PAGE, followed by Western blot experiment Immunohistochemical of paraffin-embedded human colon using E-cadherin Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FAS Antibody (13098-1-AP) at a dilution of 1:50 (10x objective).Immunohistochemical of paraffin-embedded human skin using E-cadherinHeLa cells were subjected to SDS PAGE followed by Western blot with CD44 antibody Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD44Immunohistochemical of paraffin- embedded human kidney using CD146/MCAM Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using CD146/OPTIMIZE YOUR ANTIBODY EXPERIMENTS WITH PROTEINTECHRequest your free technical guide from .THE BENCHMARK IN ANTIBODIESSince the day it was founded, Proteintech has been makingall of its products to the highest standards possible whilst takingcomplete responsibility for the quality of each product.• Proteintech makes every single antibody in its 12,000+ catalog.• Each Proteintech product is unique and cannot be bought undera different label.• Antibodies are tested with siRNA treated samples todemonstrate specificity.• It works in every single species and application or get a fullmoney-back refund.Proteintech has over 12,000 antibodies in its extensivecatalog, all fully validated and available for next day delivery.CD No.Antibody NameCat. No.TypeApplicationsCD10MME/Neprilysin 18008-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD10MME/Neprilysin 10302-1-AP Rabbit Poly ELISA, FC, IHC, WBCD10MME/Neprilysin 23782-1-AP Rabbit Poly ELISA, FC, IHC CD10MME/Neprilysin 23898-1-AP Rabbit PolyELISA, FC, IF, WBCD10MME/Neprilysin 60034-3-Ig MouseMono ELISA, IHC, WBCD102ICAM210121-2-AP Rabbit Poly ELISA, IHC, WB CD104Integrin beta-421738-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD105Endoglin/CD10510862-1-AP Rabbit Poly ELISA, IHC, IP , WBCD106VCAM-111444-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD107a LAMP121997-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD107a LAMP155273-1-AP Rabbit Poly ELISA, IF, WB CD109CD10918715-1-AP Rabbit Poly ELISA, IP , WB CD112Nectin 210100-2-AP Rabbit Poly ELISA, FC, IF, WB CD113PVRL3, Nectin 311213-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD114 G-CSFR 18310-1-AP Rabbit Poly ELISA, FC, WB CD116CSF2RA/CD11618307-1-AP Rabbit Poly ELISA, IHC, WB CD117c-Kit/CD11718696-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD118LIFR 22779-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD119IFNGR110808-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD11b CD11B/Integrin alpha M 20991-1-AP Rabbit Poly ELISA, FC, IHC, WB CD11bCD11B/Integrin alpha M21851-1-APRabbit PolyELISA, IHCCD No.Antibody NameCat. No.TypeApplicationsCD11c CD11c/Integrin alpha X 17342-1-AP Rabbit PolyELISA, FC, IHC, WBCD11c CD11c/Integrin alpha X 60258-1-Ig MouseMono ELISA, IHC, WBCD11d CD11d/Integrin alpha D 55162-1-AP Rabbit Poly ELISA, WB CD120a TNFR121574-1-AP Rabbit PolyELISA, FC, IF, IHC, WBCD120a TNFR160192-1-Ig Mouse Mono ELISA, FC, IHC, WB CD120b TNFR219272-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD121b IL1R213591-1-AP Rabbit PolyELISA, WBCD121b IL1R260262-1-Ig MouseMono ELISA, IF, WBCD122IL2RB 13602-1-AP Rabbit Poly ELISA, WB CD123IL3RA 13655-1-AP Rabbit Poly ELISA, WB CD125IL5RA 12655-1-AP Rabbit Poly ELISA, IF, IHC, WBCD126IL6R 23457-1-AP Rabbit Poly ELISA, IP , WB CD127CD127/IL7R 17626-1-AP Rabbit Poly ELISA, FC, IP , WB CD128CXCR155450-1-AP Rabbit Poly ELISA, IP , WB CD13CD1314553-1-AP Rabbit Poly ELISA, FC, IHC, WBCD132IL2RG 11409-1-APRabbit Poly ELISA, FC, WB CD133CD13318470-1-APRabbit Poly ELISA, FC, IF, IHC, WB CD133CD13318495-1-AP Rabbit Poly ELISA, IHC, WB CD133CD133-1, 2, 4, 719945-1-AP Rabbit Poly ELISA, IHC, IP , WBCD133CD133-1, 219946-1-AP Rabbit Poly ELISA, IP , WB CD134CD134/OX4020006-1-APRabbit PolyELISA, FC, WBA NTIBODY PRODUCT LIST233621117232111210CD No.Antibody NameCat. No.TypeApplicationsCD135FLT321049-1-AP Rabbit Poly ELISA, IP , WB CD136RON, MST1R 11053-1-APRabbit Poly ELISA, WB CD138CD138/ Syndecan-110593-1-APRabbit PolyCoIP ,Dot Blotting, ELISA, IF, IHC, WBCD138CD138/ Syndecan-160185-1-Ig MouseMonoELISA, WBCD138CD138/ Syndecan-160185-2-Ig MouseMono ELISA, IHC, WBCD14CD1417000-1-AP Rabbit PolyELISA, FC, IF, IHC, WBCD14CD1460253-1-Ig Mouse Mono ELISA, IF, IHC, WB CD140b PDGFRB13449-1-AP Rabbit Poly ELISA, FC, WB CD141Thrombomodulin 14318-1-AP Rabbit Poly ELISA, FC, IHC, WBCD146CD146/MCAM 17564-1-AP Rabbit PolyELISA, FC, IF, IHC, IP , WBCD146CD146/MCAM 66153-1-Ig Mouse Mono ELISA, FC, IHC, WB CD147CD14711989-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD148PTPRJ 55123-1-AP Rabbit Poly ELISA, IHC, WB CD15FUT419497-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD150SLAM/CD15010949-2-AP Rabbit Poly ELISA, WB CD151CD15110418-1-AP Rabbit Poly ELISA, IP , WB CD154CD15416668-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD156b ADAM17-Specific 20259-1-AP Rabbit Poly ELISA, IHC, WB CD159a NKG2A 10935-1-AP Rabbit Poly ELISA, IHC, WB CD164CD16412083-2-AP Rabbit Poly ELISA, FC, IHC, WBCD166ALCAM 21972-1-AP Rabbit Poly ELISA, IHC, WB CD167DDR110536-1-AP Rabbit Poly ELISA, IHC, WB CD168HMMR-Specific 15820-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD170SIGLEC513230-1-APRabbit PolyELISA, WBCD No.Antibody NameCat. No.TypeApplicationsCD172a SIRP alpha 14482-1-AP Rabbit Poly ELISA, WB CD172b SIRP BETA 111811-1-AP Rabbit Poly ELISA, IHC, WB CD172g SIRP gamma 11857-1-AP Rabbit Poly ELISA, WB CD181CXCR155450-1-AP Rabbit Poly ELISA, IP , WB CD182CXCR220634-1-AP Rabbit PolyELISA, IF, IHC, IP , WBCD183CXCR3B-specific 60065-1-Ig Mouse Mono ELISA, FC, IF, IHC, WB CD184CXCR411073-2-AP Rabbit PolyELISA, IP , WBCD184CXCR460042-1-Ig MouseMono ELISA, FC, WBCD192CCR2-specific 16153-1-AP Rabbit Poly ELISA, FC, IHC, WBCD195CCR517476-1-AP Rabbit Poly ELISA, FC, WB CD197CCR755425-1-AP Rabbit Poly ELISA, FC, IHC CD1a CD1A 17325-1-AP Rabbit PolyELISA, WBCD1a CD1A 60173-1-Ig MouseMono ELISA, WBCD2CD210299-1-AP Rabbit PolyELISA, FC, IHC, WBCD2CD260005-2-Ig MouseMonoELISA, IHC, WBCD20CD2060271-1-Ig Mouse Mono ELISA, FC, IHC, WB CD201EPCR 11391-1-AP Rabbit Poly ELISA, IHC, WB CD202b Tie219157-1-AP Rabbit Poly ELISA, IP , WB CD206CD20618704-1-AP Rabbit Poly ELISA, FC, IHC, WB CD207Langerin 11841-1-AP Rabbit Poly ELISA, WB CD208LAMP312632-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD210a IL10RA 13356-1-AP Rabbit Poly ELISA, FC, WB CD210b IL10RB 15102-1-AP Rabbit Poly ELISA, WB CD210b IL10RB 19387-1-AP Rabbit Poly ELISA, WB CD212IL12RB113287-1-APRabbit PolyELISA, IF, IP , WB3253311421411371111128Integrin beta-4More validation images available on our website.CD133CD14CD2CD No.Antibody Name Cat. No.Type ApplicationsCD213a2IL13RA211059-1-AP RabbitPoly ELISA, IF, IHC, WBCD215IL15RA16744-1-AP RabbitPoly ELISA, IHC, WBCD22CD2266103-1-Ig MouseMono ELISA, IHC, WBCD220INSR20433-1-AP RabbitPoly ELISA, IP, WBCD221IGF1R-Specific20254-1-AP RabbitPoly ELISA, IHC, IP, WBCD222IGF2R-Specific20253-1-AP RabbitPoly ELISA, FC, IF, IHC, IP, WBCD223LAG-316616-1-AP RabbitPoly ELISA, IP, WBCD225IFITM111727-3-AP RabbitPoly ELISA, FC, IF IHC, IP, WBCD225IFITM1-Specific60074-1-Ig MouseMono ELISA, FC, IF, IHC, IP, WBCD226CD22617842-1-AP RabbitPoly ELISA, IHCCD227MUC119976-1-AP RabbitPoly ELISA, FC, IHC, IP, WBCD227MUC123614-1-AP RabbitPoly ELISA, FC, IHC, WBCD228MFI210428-1-AP RabbitPoly ELISA, WBCD23CD2318642-1-AP RabbitPoly ELISA, FC, IHC, WBCD23CD2360208-1-Ig MouseMono ELISA, IHC, WBCD23CD2360208-2-Ig MouseMono ELISA, IHC, WBCD230PrP12555-1-AP RabbitPoly ELISA, FC, IHC, IP, WBCD231TSPAN718695-1-AP RabbitPoly ELISA, IHC, IP, WBCD233band 318566-1-AP RabbitPoly ELISA, IHC, IP, WBCD234DARC55185-1-AP RabbitPoly ELISA, IHC, WBCD235a Glycophorin A15874-1-AP RabbitPoly ELISA, FC, IHC, WBCD24CD2418330-1-AP RabbitPoly ELISA, WBCD24CD2410600-1-AP RabbitPoly ELISA, IHC, WBCD244CD24416677-1-AP RabbitPoly ELISA, IHCCD247CD24712837-2-AP RabbitPoly ELISA, FC, IHC,IP, WBCD No.Antibody Name Cat. No.Type ApplicationsCD248TEM118160-1-AP RabbitPolyELISA, FC, IF,IHC, WBCD249ENPEP17655-1-AP RabbitPoly ELISA, FC, IFCD253TRAIL17235-1-AP RabbitPoly ELISA, IF, IP, WBCD254RANKL23408-1-AP RabbitPolyELISA, IHC, IP,WBCD255TNFSF1212537-1-AP RabbitPoly Not TestedCD256TNFSF1310680-1-AP RabbitPolyCoIP, ELISA, IF,WBCD257TNFSF13B11862-1-AP RabbitPoly ELISA, WBCD258TNFSF1414194-1-AP RabbitPoly Not TestedCD26DPP410940-1-AP RabbitPolyELISA, IF, IHC,WBCD261DR424063-1-AP RabbitPoly ELISA, WBCD262DR5-Specific15497-1-AP RabbitPoly ELISA, WBCD264DcR216781-1-AP RabbitPoly ELISA, IHC, WBCD267TNFRSF13B21520-1-AP RabbitPoly ELISA, WBCD27CD2711121-1-AP RabbitPoly ELISA, FC, WBCD271p75NTR55014-1-AP RabbitPolyELISA, FC, IF,IHC, IP, WBCD274PD-L1/CD27417952-1-AP RabbitPolyELISA, IF, IHC,IP, WBCD275ICOSLG14922-1-AP RabbitPoly ELISA, WBCD276CD27614453-1-AP RabbitPolyELISA, FC, IHC,WBCD278ICOS13338-1-AP RabbitPoly ELISA, WBCD279PD-1/CD27918106-1-AP RabbitPolyELISA, FC, IF,IHC, WBCD283TLR317766-1-AP RabbitPoly ELISA, FC, IHCCD284TLR419811-1-AP RabbitPoly ELISA, IHC, WBCD286TLR622240-1-AP RabbitPoly ELISA, WBCD29Integrin beta-112594-1-AP RabbitPoly ELISA, WBCD292BMPR1A12702-1-AP RabbitPoly ELISA, IF, IP, WBIL15RACD23TEM1RANKLMore validation images available on our website.22411232211821482131411121CD No.Antibody NameCat. No.TypeApplicationsCD295LEPR 20966-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD298A TP1B311142-1-AP Rabbit Poly ELISA, IHC, WB CD299CLEC4M 22003-1-AP Rabbit Poly ELISA, IHC, WB CD301CLEC10A/CD30113590-1-AP Rabbit Poly ELISA, WB CD303CLEC4C 17941-1-AP Rabbit PolyNot TestedCD304Neuropilin 160067-1-Ig Mouse Mono ELISA, IF, IHC, WB CD31CD3111265-1-AP Rabbit PolyELISA, FC, IF, IHC, IP , WBCD31CD3166065-1-Ig Mouse Mono ELISA, IF, IHC, WB CD316IGSF814387-1-AP Rabbit Poly ELISA, IP , WB CD317BST213560-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD318CDCP112754-1-AP Rabbit Poly ELISA, FC, WB CD319SLAMF712905-1-AP Rabbit Poly ELISA, WB CD320CD32010343-1-AP Rabbit Poly ELISA, FC, IHC, WB CD322JAM212972-1-APRabbit Poly ELISA, WB CD324E-cadherin 20874-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD325N-cadherin 13769-1-AP Rabbit Poly ELISA, IF, IHC, WBCD325N-cadherin 22018-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD326EPCAM 21050-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD327SIGLEC613454-1-AP Rabbit Poly ELISA, IHC, WB CD328SIGLEC713939-1-AP Rabbit Poly ELISA, FC, IHC, WBCD329SIGLEC913377-1-AP Rabbit Poly ELISA, IHC, WB CD32a CD3215625-1-AP Rabbit Poly ELISA, FC, WB CD33CD3317425-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD332FGFR213042-1-AP Rabbit Poly ELISA, IF, IHC, WBCD334FGFR411098-1-APRabbit PolyELISA, IF, IHC, WBCD No.Antibody NameCat. No.TypeApplicationsCD336CD33655118-1-AP Rabbit Poly ELISA, WB CD338BCRP/ABCG210051-1-AP Rabbit Poly ELISA, IHC, WB CD34CD3414486-1-AP Rabbit Poly ELISA, IF, IHC, WBCD340HER2/ErbB218299-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD340HER2/ErbB251105-1-AP Rabbit Poly ELISA, IF, IHC, WB CD349Frizzled 913865-1-AP Rabbit Poly ELISA, WB CD350Frizzled 1018175-1-AP Rabbit Poly ELISA, IHC, WB CD354TREM111791-1-APRabbit Poly ELISA, FC, IF, WB CD36CD3618836-1-APRabbit Poly ELISA, FC, IF, IHC, WB CD360IL21R 10533-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD363S1PR1/EDG155133-1-AP Rabbit Poly ELISA, IHC, IP , WBCD37CD3721044-1-AP Rabbit PolyELISA, IHC, WBCD38CD3860006-1-Ig MouseMono ELISA, IF, WBCD39CD3919229-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD3d CD3 delta 16669-1-AP Rabbit PolyELISA, FC, IHC, IP , WBCD3d CD3 delta 60194-1-Ig MouseMono ELISA, WBCD3e CD3 epsilon 17617-1-AP Rabbit PolyELISA, FC, IF, IHC, IP , WBCD3e CD3 epsilon 60181-1-Ig Mouse Mono ELISA, FC, IHC, WB CD3g CD3 gamma 21120-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD4CD411056-2-AP Rabbit Poly ELISA, IHC, IP , WBCD4CD419068-1-AP Rabbit Poly ELISA, IP , WB CD42a GP914564-1-AP Rabbit Poly ELISA, IHC, WB CD42b CD42b 12860-1-AP Rabbit Poly ELISA, IHC, WB CD43CD4313959-1-APRabbit Poly ELISA, IHC, WB CD44CD4415675-1-APRabbit PolyELISA, FC, IF, IHC, IP , WB1222211332312452267251118616CD31HER2/ErbB2CD38LEPR E-cadherin TREM1More validation images available on our website.CD No.Antibody NameCat. No.Type ApplicationsCD44CD4460224-1-IgMouse Mono ELISA, FC, IF, IHC, WB CD45CD4520103-1-APRabbit Poly ELISA, FC, IHC, IP , Polymer Detection Kit, WBCD46CD4612494-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD47CD4720305-1-APRabbit PolyELISA, IHC, WBCD49c Integrin alpha-366070-1-Ig MouseMono ELISA, FC, IF, WBCD49c Integrin alpha-321992-1-APRabbit Poly ELISA, FC, IF, IP , WB CD49d Integrin alpha-419676-1-AP Rabbit Poly ELISA, IHC, WB CD49e Integrin alpha-510569-1-AP Rabbit Poly ELISA, IF, WBCD49f Integrin alpha 6X1A-Specific 20231-1-APRabbit Poly ELISA, WBCD49f Integrin alpha 6X1B-Specific 20232-1-AP Rabbit Poly ELISA, WB CD5CD517227-1-AP Rabbit Poly ELISA, IHC, IP , WBCD50ICAM317699-1-APRabbit Poly ELISA, FC, IHC, WBCD51Integrin beta-318309-1-APRabbit Poly ELISA, IHC, WB CD54ICAM-110831-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD55ICAM-115364-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD56NCAM1/CD5614255-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD56Icam-116174-1-AP Rabbit PolyELISA, IF, IHC, IP , WBCD56NCAM1/CD5660238-1-Ig MouseMono ELISA, IHC, WBCD58CD5810878-1-APRabbit Poly ELISA, WB CD58Icam110020-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD59CD5910742-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD61Integrin beta-318309-1-AP Rabbit Poly ELISA, IHC, WBCD62E E-selectin/CD62E 20894-1-AP Rabbit Poly ELISA, FC, WBCD62P P-selectin 13304-1-AP Rabbit Poly ELISA, IHCCD66d CEACAM3-Specific 19496-1-APRabbit Poly ELISA, FC, IHCCD66e CEA 10421-1-AP Rabbit Poly ELISA, IHC CD66f PSG111809-1-AP Rabbit Poly ELISA, IF, IHC, WBCD69CD6910803-1-AP Rabbit Poly ELISA, IHC, WB CD7CD711320-1-AP Rabbit PolyELISA, IHCCD7CD760209-2-IgMouseMono ELISA, IHC, WBCD71Transferrin receptor/CD7110084-2-APRabbit PolyELISA, IF, IHC, IP , WBCD No.Antibody NameCat. No.TypeApplicationsCD72CD7213469-1-AP Rabbit Poly ELISA, WB CD73NT5E/CD7312231-1-AP Rabbit Poly ELISA, FC, IHC, WBCD75ST6GAL114355-1-AP Rabbit Poly ELISA, IHC, IP , WBCD79a CD79A 22349-1-AP Rabbit Poly ELISA, IHC, WB CD80CD8014292-1-AP Rabbit Poly ELISA, FC, WB CD81CD8118250-1-AP Rabbit Poly ELISA, FC, IHC CD82CD8210248-1-AP Rabbit Poly ELISA, IHC, WB CD84CD8412566-1-AP Rabbit Poly ELISA, WB CD85CLILRB512881-1-AP Rabbit Poly ELISA, WB CD85FLILRA518821-1-AP Rabbit Poly ELISA, FC, WB CD85G LILRA421153-1-AP Rabbit Poly ELISA, WB CD85H LILRA211461-1-AP Rabbit Poly ELISA, IHC, WB CD86CD8613395-1-AP Rabbit Poly ELISA, FC, IHC, WBCD87uPAR 10286-1-AP Rabbit Poly ELISA, FC, IF;FC, IP , WB CD88C5AR121316-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD89CD8913245-1-AP Rabbit Poly ELISA, WB CD8b CD8B 21256-1-AP Rabbit Poly ELISA, WB CD9CD920597-1-AP Rabbit PolyELISA, FC, IHC, WBCD9CD960232-1-Ig MouseMono ELISA, IHC, WBCD94CD9413332-1-APRabbit Poly ELISA, IHC, WB CD95FAS/CD9513098-1-APRabbit PolyELISA, FC, IF, IHC, IP , WBCD95FAS/CD9560196-1-Ig Mouse Mono ELISA, FC, IHC, WB CD97CD9713071-1-AP Rabbit Poly ELISA, FC, IHC, WBCD98CD9815193-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD99CD9923079-1-AP Rabbit Poly ELISA, FC, IHC, WB CDw92SLC44A114687-1-APRabbit PolyELISA, WB1111111321175221173115431322111 THE BENCHMARKIN VALIDATIONProteintech take complete responsibility for every product theymanufacture and ensure that it will work in your hands with anextensive validation process.Here’s what you can expect:• Every antibody has been extensively validated by the in-houseteam in a wide range of applications.• Antibodies now tested in siRNA knockdown todemonstrate specificity.• Original validation data available to researcher on request.• Multi-lingual technical support available online and by email.Please Note: All products featured in this product focus are for research use only.LIVE CHAT TWITTER@proteintechBLOG /news/blogYOUTUBE /ProteintechSupport Available 24 hours via Live Chat and 9-5 (CDT) via phone. Email support also available. 1 (888) 4PTGLAB(1-888-478-4522) (toll free in USA),or 1(312) 455-8498 (outside USA)1 (312) 455-8408Proteintech Group, Inc. 5400 Pearl Street, Suite 300, Rosemont, IL 60018, USA**********************PHONE FAX ADDRESS EMAIL PHONE EMAIL FAX ADDRESS EMAIL +44 (161) 8393007+44 (161) 2413103Proteintech Europe, Ltd. 4th Floor, 196 Deansgate, Manchester, M3 3WF***********************************Sales and technical support only.PHONE FAX ADDRESS EMAIL ************or 4006-900-926************Wuhan Sanying Biotechnologies D3-3, No.666 Gaoxin Avenue, Wuhan East Lake Hi-tech Development Zone Wuhan, Hubei, P .R.C******************We are ISO 9001 and ISO 13485 accredited.Proteintech Group US Head Office Proteintech Europe United Kingdom Proteintech Europe Germany Proteintech China Office CONTACT US。