基因沉默原理

目录RNAi及基因沉默原理百奥迈科（Biomics）RNAi产品与服务一览1.siRNA生物合成1.1 全位点siRNA表达文库构建服务1.2 RNAi载体构建技术服务1.3 Gene Target TM siRNA Set2.siRNA化学合成2.1 化学合成siRNA简介2.2 siRNA设计2.3 百奥迈科(Biomics) siRNA产品简介2.4 普通siRNA oligo2.5 荧光标记siRNA oligo2.6 化学修饰siRNA oligo2.7 miRNA的化学合成2.8 RNAi系列优惠套餐2.9 siRNA对照3.RNAi病毒包装3.1 腺病毒载体包装3.2 慢病毒载体包装3.3 其他类型病毒载体包装4.哺乳动物细胞siRNA转染4.1 转染方法4.2 转染步骤4.3 转染优化及注意事项5.mRNA水平检测基因沉默5.1 mRNA的提取/制备5.2. 实时定量荧光PCR6蛋白质水平检测基因沉默6.1 Western blot6.2 ELISA6.3 免疫荧光7.其他相关服务7.1 稳定细胞筛选服务7.2 细胞增殖毒性检测服务7.3 细胞凋亡检测服务7.4 DNA测序8.RNAi相关产品8.1 RISO TM RNA抽提试剂8.2 其他RNAi及基因沉默原理RNA干扰(RNAinterference，RNAi)是由双链RNA (double-strandedRNA，dsRNA) 引发的转录后基因静默机制。

其原理是：RNaseIII核酶家族的Dicer，与双链RNA结合，将其剪切成21 - 25nt及3'端突出的小干扰RNA (small interfering RNA，siRNA)，随后siRNA与RNA 诱导沉默复合物(RNA - induced silencing complex，RISC) 结合，解旋成单链，活化的RISC 受已成单链的siRNA引导，序列特异性地结合在靶mRNA上并将其切断，引发靶mRNA的特异性分解，从而阻断相应基因表达的转录后基因沉默机制。

百奥迈科（Biomics）RNAi产品与服务一览1.siRNA 生物合成1.1全位点siRNA表达文库构建服务本公司拥有自主知识产权的全位点siRNA文库构建技术，能够从全长cDNA构建包含所有有效结合位点，长度分布于19 - 23bp的靶基因的siRNA分子库，亦可以模仿体内Dicer酶切割长度为21 - 23bp的分子库，适用于高通量功能基因及相关药物靶点筛选。

1.1.1构建流程：1）客户可选：z提供靶基因的cDNA克隆。

z提供靶基因的相关信息(基因的mRNA序列或Genbank Accession Number)。

z也可在本公司的cDNA产品目录中，挑选靶基因cDNA克隆。

2）进行siRNA文库构建，抽样测序验证。

3）根据客户要求提供相应的文库产品。

4）将其连同抽样报告、产品说明书一起寄送到客户手中。

A.HBV Polymerase Domain siRNA表达文库位点分布（随机抽样测序）Polymerase Domain (984bp):B.构建的Survivin基因全位点siRNA表达文库，文库抽样基因沉默(qPCR)筛选结果：该数据显示，本公司siRNA文库位点分布的随机性，可以更好地找到最优的靶点。

s1-s8: Randomly selected siRNA sequences from Biomics librarys9/s10/s11: The published siRNA sequencestarget-off: Negative controlun-transfect: Untreated normal control1.2RNAi载体构建技术服务1.2.1技术特点：1）多种siRNA表达载体，可以在细胞内直接转录出siRNA，行使其RNAi功能，包括：z U6启动子表达载体；z H1启动子表达载体；z U6、H1双启动子表达载体；z CMV启动子表达载体；2）载体带有GFP标签可以监测转染效率。

构建好的siRNA表达载体可直接用于转染细胞进行RNAi实验;3）本公司还可根据客户需要提供各种商业化表达载体;4）本公司可以为客户构建线性siRNA表达载体，shRNA表达载体，miRNA表达载体以及病毒型RNAi载体。

1.2.2构建流程：1）siRNA靶点设计；z客户提供靶基因的siRNA序列，由本公司将其克隆到siRNA表达载体上。

z客户提供靶基因的相关信息（基因的mRNA序列或Genbank Accession No.），根据客户要求，本公司设计高效特异性的3-4条针对靶基因的siRNA。

2）合成设计好的DNA并退火形成双链DNA，克隆到客户要求的载体上。

3）通过测序进行验证。

4）构建成功的siRNA质粒连同测序报告、产品说明书一起寄送到客户手中。

注：可根据客户要求提供siRNA的阳性及阴性对照质粒。

1.2.3质量保证：符合设计要求的siRNA序列测序结果。

基于载体的RNAi实验示意图1.2.4各种商业化表达载体:产品编号载体启动子筛选基因标记基因BV0011 pRNAi-U6H1/Neo U6和H1 Neomycin GFP BV0012 pRNAi-H1.1/Neo H1 Neomycin GFP BV0013 pRNAi-U6.1/Neo U6 Neomycin GFPCMV Neomycin GFP BV0014 pRNAi-CMV3.1/NeoCMV Neomycin GFP BV0015 pRNAi-CMV3.2/NeoCMV Neomycin - BV0016 pRNAi-CMV4.1/NeoBV0107 pRNAi-U6.1/Lenti U6 Neomycin GFP BV0108 pRNAi-H1.1/Retro H1 Neomycin GFP1.3. Gene Target TM siRNA SetGene Target™ siRNA Set是本公司的专利技术开发的基因沉默系列产品之一。

产品由4个不同位点的siRNA克隆组成，可以降低在基因沉默中产生的脱靶现象，提高沉默效率。

四个克隆可以混合使用，也可以单独使用。

产品均经基因测序证实，保证其100%的同源性。

本产品有如下特点：z在U6和H1启动子的起始序列AAAAA和终止序列TTTTT之间的siRNA片段没有多余的多克隆位点序列，提高沉默的特异性。

z siRNA 表达框（U6－siRNA－H1）可以整体亚克隆到逆转录病毒载体 (Retrovirus)、腺病毒载体（Adenovirus）、慢病毒载体（Lentivirus），具有较好的适用性。

z siRNA 没有二级结构(如发夹型结构)，在上述病毒载体中较稳定。

z构建的质粒载体转染到细胞内，在细胞内转录出双链RNA，在细胞内具有中长期表达的特性，能够较长期的发挥基因沉默的作用。

z由于质粒可以复制扩增，较化学合成方法显著降低制备siRNA的成本。

z载体法的siRNA可测序保证100%序列同源性，而化学合成的siRNA分子的序列鉴定较困难。

表达载体pU6H1-GFP图谱产品性状形态：本产品具有可靠的稳定性，以质粒DNA与滤纸小圆片共干燥封装，可在常温下运输和储存。

Gene Target TM siRNA Set产品包含： 1. 特定基因4个位点的Gene Target TM siRNA Clone。

2. 阳性对照1管（用于平行跟踪转染、RNA提取等实验过程，排除假阴性结果。

） 3. 阴性对照1管（阴性对照的siRNA与目的基因以及靶细胞中其它基因mRNA没有同源性，排除假阳性结果。

）Gene Target TM siRNA Set产品目录KinaseK07002 NM_145262 Homo sapiens glycerate kinase (GLYCTK)K07003 NM_145259 Homo sapiens activin A receptor, type IC (ACVR1C)K07004 NM_153361 Homo sapiens hypothetical protein MGC42105 (MGC42105)K07005 NM_153273 Homo sapiens inositol hexaphosphate kinase 1 (IHPK1), transcript variant 1K07007 NM_152720 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3)K07008 NM_030906 Homo sapiens serine/threonine kinase 33 (STK33)K07009 NM_020804 Homo sapiens protein kinase C and casein kinase substrate in neurons 1 (PACSIN1)K07012 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G), transcript variant 3 K07016 NM_152835 Homo sapiens PDLIM1 interacting kinase 1 like (PDIK1L)K07021 NM_015000 Homo sapiens serine/threonine kinase 38 like (STK38L)K07026 NM_174858 Homo sapiens adenylate kinase 5 (AK5), transcript variant 1K07027 NM_173354 Homo sapiens SNF1-like kinase (SNF1LK)K07029 NM_033015 Homo sapiens Fas-activated serine/threonine kinase (FASTK), transcript variant 4K07030 NM_032844 Homo sapiens microtubule associated serine/threonine kinase-like (MASTL)K07033 BC032542 Homo sapiens, L-fucose kinase, clone MGC:45494 IMAGE:5534376, complete cdsK07035 NM_032028 Homo sapiens testis-specific serine kinase 1 (TSSK1)K07041 NM_031414 Homo sapiens serine/threonine kinase 31 (STK31), transcript variant 1K07046 NM_182691 Homo sapiens SFRS protein kinase 2 (SRPK2), transcript variant 2K07054 NM_032409 Homo sapiens PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial proteinK07055 NM_023018 Homo sapiens NAD kinase (NADK)K07056 NM_024876 Homo sapiens aarF domain containing kinase 4 (ADCK4)K07057 NM_006153 Homo sapiens NCK adaptor protein 1 (NCK1)K07059 NM_006293 Homo sapiens TYRO3 protein tyrosine kinase (TYRO3)‐ 8 ‐K07060 NM_024761 Homo sapiens MOB1, Mps One Binder kinase activator-like 2B (yeast) (MOBKL2B)K07061 NM_173515 Homo sapiens CNKSR family member 3 (CNKSR3)K07066 NM_002497 Homo sapiens NIMA (never in mitosis gene a)-related kinase 2 (NEK2)Gene Target TM siRNA Set产品目录K07067 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN)K07069 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB),K07071 NM_020421 Homo sapiens aarF domain containing kinase 1 (ADCK1)K07076 NM_171825 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A),K07079 NM_001798 Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1K07080 NM_004936 Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1K07082 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3),K07085 NM_005233 Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1K07087 NM_004103 Homo sapiens PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 2K07089 NM_000801 Homo sapiens FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 12BK07090 NM_032999 Homo sapiens general transcription factor II, i (GTF2I), transcript variant 1K07091 NM_030952 Homo sapiens NUAK family, SNF1-like kinase, 2 (NUAK2)K07092 NM_000221 Homo sapiens ketohexokinase (fructokinase) (KHK), transcript variant aK07093 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3),K07096 NM_181839 Homo sapiens protein kinase (cAMP-dependent, catalytic) inhibitor alpha (PKIA), transcript variant 7K07100 NM_145160 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant AK07102 NM_002822 Homo sapiens twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1)K07105 NM_003583 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 1 K07108 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 K07109 NM_139078 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 2 K07110 NM_030662 Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)‐ 9 ‐K07111 NM_025233 Homo sapiens Coenzyme A synthase (COASY), transcript variant 1K07112 NM_170693 Homo sapiens serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1K07116 NM_007194 Homo sapiens CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1K07118 NM_017593 Homo sapiens BMP2 inducible kinase (BMP2K), transcript variant 2K07121 NM_004336 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) K07122 NM_005308 Homo sapiens G protein-coupled receptor kinase 5 (GRK5)Gene Target TM siRNA Set产品目录K07125 NM_002648 Homo sapiens pim-1 oncogene (PIM1)K07126 NM_005030 Homo sapiens polo-like kinase 1 (Drosophila) (PLK1)K07128 NM_006374 Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25)K07130 NM_021643 Homo sapiens tribbles homolog 2 (Drosophila) (TRIB2)K07131 NM_013254 Homo sapiens TANK-binding kinase 1 (TBK1)K07132 NM_020247 Homo sapiens chaperone, ABC1 activity of bc1 complex homolog (S. pombe) (CABC1), nuclear gene encoding mitochondrial proteinK07133 NM_144685 Homo sapiens homeodomain interacting protein kinase 4 (HIPK4)K07136 NM_002752 Homo sapiens mitogen-activated protein kinase 9 (MAPK9), transcript variant JNK2-a2K07137 NM_024046 Homo sapiens CaM kinase-like vesicle-associated (CAMKV)K07138 NM_021913 Homo sapiens AXL receptor tyrosine kinase (AXL), transcript variant 1K07141 NM_021135 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 2 (RPS6KA2), transcript variant 1K07142 NM_021158 Homo sapiens tribbles homolog 3 (Drosophila) (TRIB3)K07143 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4)K07144 NM_020168 Homo sapiens p21(CDKN1A)-activated kinase 6 (PAK6)K07145 NM_018571 Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2 (ALS2CR2) K07146 NM_018492 Homo sapiens PDZ binding kinase (PBK)K07148 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B)‐ 10 ‐K07150 NM_018343 Homo sapiens RIO kinase 2 (yeast) (RIOK2)K07151 NM_017988 Homo sapiens SCY1-like 2 (S. cerevisiae) (SCYL2)K07152 NM_017719 Homo sapiens SNF related kinase (SNRK)K07154 NM_016281 Homo sapiens TAO kinase 3 (TAOK3)K07155 NM_016123 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4)K07158 NM_014683 Homo sapiens unc-51-like kinase 2 (C. elegans) (ULK2)K07160 NM_014413 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1)K07161 NM_014365 Homo sapiens heat shock 22kDa protein 8 (HSPB8)K07162 NM_014264 Homo sapiens polo-like kinase 4 (Drosophila) (PLK4)Gene Target TM siRNA Set产品目录K07163 NM_013257 Homo sapiens serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1 K07164 NM_007284 Homo sapiens twinfilin, actin-binding protein, homolog 2 (Drosophila) (TWF2)K07166 NM_007199 Homo sapiens interleukin-1 receptor-associated kinase 3 (IRAK3)K07168 NM_006712 Homo sapiens Fas-activated serine/threonine kinase (FASTK), transcript variant 1K07169 NM_006622 Homo sapiens polo-like kinase 2 (Drosophila) (PLK2)K07170 NM_006281 Homo sapiens serine/threonine kinase 3 (STE20 homolog, yeast) (STK3)K07171 NM_006285 Homo sapiens testis-specific kinase 1 (TESK1)K07172 NM_006296 Homo sapiens vaccinia related kinase 2 (VRK2)K07173 NM_006296 VARIANT 1 of Homo sapiens vaccinia related kinase 2 (VRK2)K07174 NM_006252 Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)K07175 NM_212539 Homo sapiens protein kinase C, delta (PRKCD), transcript variant 2K07176 NM_006255 Homo sapiens protein kinase C, eta (PRKCH)K07177 NM_006213 Homo sapiens phosphorylase kinase, gamma 1 (muscle) (PHKG1)K07178 NM_006182 Homo sapiens discoidin domain receptor family, member 2 (DDR2), transcript variant 2K07179 NM_005990 Homo sapiens serine/threonine kinase 10 (STK10)‐ 11 ‐K07180 NM_005923 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)K07181 NM_005881 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK)K07182 NM_005762 Homo sapiens tripartite motif-containing 28 (TRIM28)K07183 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK)K07185 NM_000020 Homo sapiens activin A receptor type II-like 1 (ACVRL1), transcript variant 1 K07186 NM_005433 Homo sapiens v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)K07188 NM_005211 Homo sapiens colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog (CSF1R)K07190 NM_005044 Homo sapiens protein kinase, X-linked (PRKX)K07192 NM_004760 Homo sapiens serine/threonine kinase 17a (apoptosis-inducing) (STK17A)K07194 NM_004517 Homo sapiens integrin-linked kinase (ILK), transcript variant 1K07195 NM_004443 Homo sapiens EPH receptor B3 (EPHB3)Gene Target TM siRNA Set产品目录K07197 NM_004409 Homo sapiens dystrophia myotonica-protein kinase (DMPK)K07198 NM_004226 Homo sapiens serine/threonine kinase 17b (apoptosis-inducing) (STK17B)K07200 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1), transcript variant 1K07201 NM_004073 Homo sapiens polo-like kinase 3 (Drosophila) (PLK3)K07202 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2),K07203 NM_003954 Homo sapiens mitogen-activated protein kinase kinase kinase 14 (MAP3K14)K07204 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2)K07208 NM_003318 Homo sapiens TTK protein kinase (TTK)K07210 NM_003177 Homo sapiens spleen tyrosine kinase (SYK)K07211 NM_003188 Homo sapiens mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant A K07212 NM_003137 Homo sapiens SFRS protein kinase 1 (SRPK1)K07214 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4)‐ 12 ‐K07219 NM_002969 Homo sapiens mitogen-activated protein kinase 12 (MAPK12)K07221 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)K07223 NM_002821 Homo sapiens PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-1 K07225 NM_002758 Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6)K07226 NM_002732 Homo sapiens protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) K07229 NM_002741 Homo sapiens protein kinase N1 (PKN1), transcript variant 2K07232 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), transcript variant 1K07233 NM_002748 Homo sapiens mitogen-activated protein kinase 6 (MAPK6)K07234 NM_002751 Homo sapiens mitogen-activated protein kinase 11 (MAPK11)K07235 NM_002610 VARIANT 1 of Homo sapiens pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial proteinK07236 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein K07237 NM_002611 Homo sapiens pyruvate dehydrogenase kinase, isozyme 2 (PDK2)K07239 NM_002613 VARIANT 1 of Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1),K07240 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 1Gene Target TM siRNA Set产品目录K07241 NM_002376 VARIANT 1 of Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3)K07242 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3)K07243 NM_002093 VARIANT 1 of Homo sapiens glycogen synthase kinase 3 beta (GSK3B)K07245 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B)K07246 NM_002005 Homo sapiens feline sarcoma oncogene (FES)K07248 NM_001892 Homo sapiens casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2K07249 NM_001799 Homo sapiens cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) (CDK7)K07251 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK)K07252 NM_001721 Homo sapiens BMX non-receptor tyrosine kinase (BMX)‐ 13 ‐K07253 NM_001744 Homo sapiens calcium/calmodulin-dependent protein kinase IV (CAMK4)K07255 NM_001619 Homo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1)K07256 NM_001319 Homo sapiens casein kinase 1, gamma 2 (CSNK1G2)K07258 NM_001261 Homo sapiens cyclin-dependent kinase 9 (CDC2-related kinase) (CDK9)K07260 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1)K07261 NM_001203 Homo sapiens bone morphogenetic protein receptor, type IB (BMPR1B)K07262 NM_001211 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B)K07263 NM_001105 Homo sapiens activin A receptor, type I (ACVR1)K07264 NM_000459 Homo sapiens TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal) (TEK) K07266 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2)K07268 NM_000222 Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT)K07269 NM_052841 Homo sapiens testis-specific serine kinase 3 (TSSK3)K07271 NM_139033 Homo sapiens mitogen-activated protein kinase 7 (MAPK7), transcript variant 1K07281 NM_005424 Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)K07282 NM_000906 Homo sapiens natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (NPR1)K07285 NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1K07286 NM_001315 Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 1K07287 NM_172115 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D), transcript variant 4Gene Target TM siRNA Set产品目录K07290 NM_002595 Homo sapiens PCTAIRE protein kinase 2 (PCTK2)K07294 NM_003331 Homo sapiens tyrosine kinase 2 (TYK2)K07295 NM_003993 Homo sapiens CDC-like kinase 2 (CLK2), transcript variant 1K07296 NM_001954 Homo sapiens discoidin domain receptor family, member 1 (DDR1), transcript variant 2K07297 NM_172127 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D), transcript variant 1 K07298 NM_003215 Homo sapiens tec protein tyrosine kinase (TEC)‐ 14 ‐K07299 NM_139355 Homo sapiens megakaryocyte-associated tyrosine kinase (MATK),transcript variant 1VirusV07001 M38454 Hepatitis B virus , complete genome, CDS：1976..2656V07003 M38454 Hepatitis B virus , complete genome, CDS：501..1058V07005 NC_001526 Human papillomavirus - 16, complete genomeV07006 NC_001357 Human papillomavirus - 18, complete genomeV07007 AY536759 SARS coronavirus BJ01 membrane protein mRNA, complete cdsV07008 AY676046 Influenza A virus strain (A/egret/Hong Kong/757.2/03(H5N1))membrane protein (M) gene, complete cdsV07009 DQ826727 HIV-1 isolate 2931HA from Cameroon gag protein (gag) and pol protein (pol) genes, partial cds; and vif protein (vif), vpr protein (vpr), tat protein (tat), rev protein (rev), vpu protein (vpu), envelope glycoprotein (env), and nef protein (nef) genes, coV07010 NC_001563 West Nile virus, complete genome,Envelope proteinV07011 NC_001563 West Nile virus, complete genome,RNA-dependent RNA polymerase V07013 NC_001563 West Nile virus, complete genome,non-structural proteinOthersM06022 NM_012131 Homo sapiens claudin 17 (CLDN17)M06061 BC013589 Homo sapiens myeloid differentiation primary response gene (88),mRNA (cDNA clone MGC:9601 IMAGE:3900951), complete cdsGene Target TM siRNA Set产品目录M06063 NM_173394 Mus musculus TRIF-related adapter molecule TRAM (Tram)M06085 NM_001018055 Homo sapiens BRCA1/BRCA2-containing complex, subunit 3 (BRCC3),transcript variant 2 M06103 NM_006472 Homo sapiens thioredoxin interacting protein (TXNIP)‐ 15 ‐M06119 BC000052 Homo sapiens peroxisome proliferator-activated receptor alphaM06235 NM_199290 Homo sapiens nascent polypeptide-associated complex alpha subunit 2 (NACA2)M06236 NM_032339 Homo sapiens chromosome 17 open reading frame 37 (C17orf37)M06237 NM_005564 Homo sapiens lipocalin 2 (oncogene 24p3) (LCN2)M06239 NM_001106 Homo sapiens activin A receptor, type IIB (ACVR2B)M06245 NM_005438 Homo sapiens FOS-like antigen 1 (FOSL1)M06246 NM_005253 Homo sapiens FOS-like antigen 2 (FOSL2)M06278 NM_005594 Homo sapiens nascent polypeptide-associated complex alpha subunit(NACA)M06310 NM_004095 Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) BM07130 NM_138294 Homo sapiens expressed in prostate and testis (PATE)‐ 16 ‐2. siRNA化学合成2.1化学合成siRNA简介化学合成siRNA是应用起来最方便的方法，客户只需提供siRNA序列或者GeneID、Accession Number、基因名称等。