生物梅里埃API说明书_part1(1-49)

/
/
/
/
(Chryseobacterium indologenes/ Pseudomonas spp/ aeruginosa
/fluorescens / putida)
(Chryseobacterium indologenes)
(Proteus vulgaris)
(Stenotrophomonas maltophilia)
bioMérieux sa
11
api 10S
08052E-XL-CN-11/2000
0 002
0 003 0 004 0 006 0 007 0 016 0 022
0 023 0 026
0 027 0 075 0 100 0 104 0 206 0 216 0 265 0 274 0 314 0 315 0 400
/
/
(Stenotrophomonas maltophilia / Shigella spp / Pseudomonas spp)
(Pseudomonas spp)
/
(Chryseobacterium meningosepticum / indologenes)
(Shewanella putrefaciens)
bioMérieux sa
-3
9
api 10S
• API 10 S •
2
(T index)
10 API 20 E •
08052E-CN-11/2000
(
)
2
(%ID)
API 20 E
•
6061
-94.39%
72.65%
-1.91%
-3.71%
• API 20 E
-
96.9%
95.9%
37467
(E.A. Robertson et al., 1975)
(Edwardsiella tarda)
/
/
/
/
/
(Stenotrophomonas maltophilia / Acinetobacter
baumannii / Pseudomonas aeruginosa / fluorescens / putida / spp)
/
/
/
(Pseudomonas aeruginosa / fluorescens / putida / spp)
api 10S
APl APl 10 S APl LISTERIA APl NH API CANDIDA APl 20 NE APl 20E APl 20 C AUX APl 20A APl STAPH APl 20 STREP RAPlD 20E APl CAMPY APl CORYNE APl ZYM APl 50 CHL APl 50 CHB/E
+
+
-
+
69280 Marcy-I'Etoile/France tel.(33) 04 78 87 20 00 /fax(33) 04 78 87 20 90 www.biomerieux.com
+
+
-
+
bioMérieux sa
1
05-02/006GB99023A/This document is not legally binding.BIOMERIUEUX reserves the right to modify specifications without.BIOMERIEUX and the blue logo.API are registered and protected trademarks belonging to biomerieux sa or one of its subsidiaries /Printed in France/THEPA McCann Healthcare/RCS Lyon B398 160 242
NaCl 0.85% NaCl 5ml
8.5g 1000ml
5ml TDA 5ml JAMES 5ml
NIT 1 5ml
NIT 2 5ml
FeCl3 H2O J 2183 HCl 1N
S 24 / 25
()
10
H2O
R34 S2 S23 S26
N, N-
H2O
R34 S2 S23 S26
3.4g 100ml
/
(Salmonella typhi / Stenotrophomonas maltophilia)
(Shewanella putrefaciens)
(Shewanella putrefaciens)
(Morganella morganii)
(Proteus mirabilis)
(Salmonella choleraesuis)
Manual Biosafety inMicrobiological and •
5-10
Biomedical Laboratories, 1993)
• 3. OXIDASE
•
•
•
08052E-CN-11/2000
) (
•
API 10 S (*) 2-8
(*)
(
)
10
(
)
( TDA NIT 1
)
2-8
/
/
/
/
/
/
(Chryseobacterium indologenes / Pseudomonas
aeruginosa / fluorescens / putida / Sphingobacterium multivorum / Pseudomonas spp)
(Chryseobacterium indologenes)
ONPG GLU ARA LDC ODC CIT H2S URE TDA IND OX NO2
1. +
+
+
+
+
-
-
-
-
+
-
+
2. +
+
+
-
+
+
-
-
-
-
-
+
3. -
+
-
-
+
V
+
+
+
-
-
+
4. +
-
-
V
-
V
-
-
-
-
-
-
+
18-20
1.
(Escherichia coli)
ATCC 25922
2.
(Enterobacter cloacae)
0.5g 100ml
0.4g 30g 70ml
0.6g 30g 70ml
bioMérieux sa
-1
7
api 10S
JAMES
•
•
•
•
•
(20-30 )
• •
-
(20-30 )
1. TDA, NIT 1,NIT 2
•
(
-
(
)
-
•1
-
•
-
* * •
2. JAMES •
•
• •
JAMES )
•
•
(NCCLS M29-A CDC •
+ -
-/+ + -
69280 Marcy-l'Etoile/France tel.(33) 04 78 87 20 00 /fax(33) 04 78 87 20 90 www.biomerieux.com
+ + +
+
2
bioMérieux sa
+
+
-
20-25 37
bioMérieux sa
69280 Marcy-l'Etoile/France tel.(33) 04 78 87 20 00 /fax(33) 04 78 87 20 90 www.biomerieux.com
3
4
API
bioMérieux sa
API
bioMérieux sa
5
REF 10 100
API 10 S
G API 10 S
G-
11
08052E-CN-11/2000
API 10 S
10
35-37
24
(50
)
-50 API 105
-50
-1
-1
-1
(
)
-0.85% NaCl
5ml (ref. 20 230)
(Chryseobacterium indologenes)
• • 35-37
18-24
• 35-37 • •
-TDA
18-24 1 TDA
-IND
1 JAMES
-NO2 2-5
GLU
NIT 1 NIT 2 1
•
10
11
12
)
•
08052E-CN-11/2000
124
API 10 S
4
(
NO2
7 504 Klebsiella pneumoniae ssp pneumoniae
08052E-XL-CN-11/2000
1 2 3 4 7 18 24 34 42 50 60 66 74 81 89 96 110 118 122 128
05-02/006GB99023A/This document is not legally binding.BIOMERIUEUX reserves the right to modify specifications without.BIOMERIEUX and the blue logo.API are registered and protected trademarks belonging to biomerieux sa or one of its subsidiaries /Printed in France/THEPA McCann Healthcare/RCS Lyon B398 160 242
/
/
/
/
/
(Pseudomonas spp / aeruginosa fluorecens
/ putida / Chryseobacterium meningoseptiicum / indologenes)
/
(Chryseobacterium meningosepticum / indologenes)
0 402 0 403 0 404 0 406
0 416 0 422
0 423 0 427 0 445 0 465 0 500 0 504 0 606 0 612 0 616 0 674 1 000 1 002 1 003 1 007 1 020 1 022 1 023 1 024 1 027 1 100 1 104 1 224 1 307
2-30
•
(
)
3ml
(
Cl2 CO2
)
• )
• API 10 S
API 10 S
(
G( Francisella)
Brucella
8
bioMérieux sa
-2
api 10S
• 0.85%NaCl 5ml
• •
(5ml)
(Vibrio) 0.85%NaCl
•
CIT
Biblioteka Baidu• LDC ODC H2S URE
-
93.5%
85.6%
201
(S.B. Phillips et al., 1977)
-
100%
70
1999
/
ONPG
-
GLU
ARA
LDC
ODC
CIT
H2S
URE
TDA
-β-D-
β-
/ (4)
/-
/ (4)
/-
/
H2S
/
TDA /
(1) /-
/ (2) / (3)
/
IND
JAMES/
OX
NO2
(GLU )
Shewanella putrefaciens
/
/
/
/
/
(Pseudomonas aeruginosa / fluorescens /
putida / Chryseobacterium indologenes / Pseudomonas spp / Sphingobacterium multivorum)
5ml (ref. 20 150)
-
TDA
(ref. 70 402)
JAMES
(ref. 70 542)
NIT 1+NIT 2 (ref. 70 442)
OXIDASE
(ref. 55 635)
-
(ref. 70 100)
-
PSI
(ref. 70 250)
-
(ref. 70 200)
-
-35-37 -
ATCC 13047
3.
(Proteus mirabilis)
ATCC 35659
4.
(Stenotrophomonas maltophilia)
ACTT 15331
ATCC American Type Culture Collection, 12301 Parklawn Drive,Rockville,Maryland 20852, USA.
bioMérieux sa 33 04 78 87 20 90
bioMérieux,Inc 595 Anglum Road,Hazelwood, Missouri 63042-2320/USA
1 314.731 8500/
1 314.731 8700
10
api 10S
35-37
18-24
08052E-XL-CN-11/2000
/
(Chryseobacterium meningosepticum / indologenes)
(Stenotrophomonas maltophilia)
/
/
/
/
(Pseudomonas spp / aeruginosa / fluorescens / putida / Shewanella
putrefaciens)
(1)
(2)
16-24
(3)
(4)
NO2
/ NIT 1 + NIT 2/2-3
p.I p.II p.VII
bioMérieux sa au capital de 77 421 420 F 673 620 399 RCS LYON 69280 Marcy-l'Etoile/France
33 04 78 87 20 00/ http://www.biomerieux.com