细胞与分子生物学词汇表

细胞与分子生物学词汇表
细胞与分子生物学词汇表

Week 1

https://www.360docs.net/doc/b76433964.html,anism [生物体An individual, composed of

organ systems (if multicellular). Multiple organisms make up a population.

2.cells The smallest structural units of

living matter capable of functioning independently.

3.protist [原生生物

4.prokaryote [原核生物Type of cell that

lacks a membrane-bound nucleus and has no membrane organelles; a bacterium.

Prokaryotes are more primitive than eukaryotes. Cells lacking membrane-bound organelles and having a single circular chromosome, and ribosomes surrounded by a cell membrane. Prokaryotes were the first forms of life on earth, evolving over 3.5 billion years ago

5.P r o k a r y o t i c原核的

6.eukaryote真核细胞A type of cell found in

many organisms including single-celled protists and multicellular fungi, plants, and animals; characterized by a membrane-bounded nucleus and other membraneous organelles; an organism composed of such cells. The first eukaryotes are encountered in rocks approximately 1.2-1.5 billion years old.

7.E u k a r y o t i c真核状态的

8. protozoa [原生生物Single-celled

protists grouped by their method of locomotion. This group includes Paramecium, Amoeba, and many other commonly observed protests

9.plasma 血浆The liquid portion of the blood.

Along with the extracellular tuid, it makes up the internal environment of multicellular organisms.

10.cell theory One of the four (or five)

unifying concepts in biology. The cell theory states that all living things are composed of at least one cell and that the cell is the fundamental unit of function in all organisms. Corollaries 必然的结果: the chemical composition of all cells is fundamentally alike; all cells arise from

preexisting cells through cell division.

11.mitosis [有丝分裂The division of the

cell's nucleus and nuclear material of a cell; consists of four stages: prophase, metaphase, anaphase, and telophase. Cell xeroxing. Mitosis occurs only in eukaryotes. The DNA of the cell is replicated during interphase of the cell cycle and then segregated during the four phases of mitosis.

12.meiosis 减数分裂Cell division in which the

chromosomes replicate, followed by two nuclear divisions. Each of the resulting gametes (in animals, spores in plants) receives a haploid set of chromosomes.

Reduction/division by which ploidy, the number of sets of homologous chromosomes, is reduced in the formation of haploid cells that become gametes (or gametophytes in plants)

13.ultracentrifuge [ 用超高速离心器

14.[metabolism新代 The sum of all chemical

reactions (energy exchanges) in cells

15.irritability [过敏性, 兴奋性, 易怒

16.homeostasis[动态静止,动态平衡The ability

to maintain a relatively constant internal environment

17.adaptation[适应Tendency of an organism to

suit its environment; one of the major points of Charles Darwin's theory of evolution by natural selection: organisms adapt to their environment. Those organisms best adapted will have a greater chance of surviving and passing their genes on to the next generation.

18.Acellular : have not cellular

organisation ,do not display all the major life attributes.

19.virus病毒Infectious chemical agent

composed of a nucleic acid (DNA or RNA) inside a protein coat

20.Viroids 类病毒are naked pieces of RNA that

lack cytoplasm, membranes, etc. They are parasites of some plants and also as possible glimpses of the functioning of pre-cellular life forms.

21.prions朊病毒, 蛋白感染素Infectious

agents composed only of one or more protein molecules without any accompanying genetic information.

22.microscope 显微镜

23.magnification 放大倍率is the ratio of

enlargement between the specimen and its image

24.Resolution 分辨率,在显微镜检时,所谓分辨

即毗连两物能识别为相互分离时的最小距离。

is the ability to distinguish between two objects.

25.Paramecium [草履虫

https://www.360docs.net/doc/b76433964.html,anelle[细胞器Cell components that

carry out individual functions; e.g., the cell nucleus and the endoplasmic reticulum.

Subcellular structures (usually membrane-bound and unique to eukaryotes) that perform some function, e.g.

chloroplast, mitochondrion, nucleus.

27.lysosomes [溶酶体Membrane-enclosed

organelles containing digestive enzymes.

The lysosomes fuse with food vacuoles and enzymes contained within the lysosome chemically breakdown and/or digest the food vacuole's contents.

28.endocytosis (细胞)吞作用The

incorporation of materials from outside the cell by the formation of vesicles in the plasma membrane. The vesicles surround the material so the cell can engulf it.

29.Hierarchical 分等级的

30.ecology[生态学,The study of how organisms

interact with each other and their physical environment。

31.population [人口A group of individuals of

the same species living in the same area at the same time and sharing a common gene pool.

A group of potentially interbreeding

organisms in a geographic area.

https://www.360docs.net/doc/b76433964.html,munity [公社, 团体, 社会All species or

populations living in the same area.

33.ecosystem [生态系统The community living in

an area and its physical environment. 34.biosphere [生物圈All ecosystems on Earth

as well as the Earth's crust, waters, and

atmosphere on and in which organisms exist;

also, the sum of all living matter on Earth.

35.nucleoside [核苷

36.glycose [单糖,

37.glucose[葡萄糖A six-carbon single sugar;

the most common energy source.

38.minimum [最小的, 最低的

39.coefficient [系数

40.metabolite [代物

41.molecules 分子Units of two or more atoms

held together by chemical bonds. The combination of atoms by chemical bonds with the component atoms in definite porportions, such as water (two H to one O).

42.Micro-organisms:organisms not visible to

naked eye。

43.primary cell wall The cell wall outside the

plasma membrane that surrounds plant cells;

composed of the polysaccharide cellulose.

44.polysaccharides [多醣Long chains of

monosaccharide units bonded together; e.g., glycogen, starch, and cellulose.

Week 2

1、isotonic solution :等溶液

2、osmosis [渗透(作用Cholesterol胆固醇: a

type of lipid called a sterol(固醇)

4、Macromolecules 大分子Large molecules made

up of many small organic molecules that are often referred to as monomers; e.g.,

carbohydrates, lipids, proteins, and

nucleic acids. Macromolecules are polymers of monomers.

5、lipids类脂One of the four classes of

organic macromolecules. Lipids function in the long-term storage of biochemical

energy, insulation, structure and control.

Examples of lipids include the fats, waxes, oils and steroids (e.g. testosterone,

cholesterol).

6、Carbohydrates 碳水化合物Organic

molecules composed of carbon, hydrogen,

and oxygen that serve as energy sources and structural materials for cells of all

organisms.

7、Glycolipids 糖脂Polysaccharides formed of

sugars linked to lipids, a part of the cell membrane.

8、Glycoproteins 糖蛋白Polysaccharides

formed of sugars linked to proteins. On the outer surface of a membrane, they act as receptors for molecular signals

originating outside the cell.

9、Hemoglobin 血红蛋白A red pigment in red

blood cells that can bind with oxygen and is largely responsible for the blood's

oxygen-carrying capacity. Hemoglobin is

composed of four polypeptide chains, two alpha () and two beta () chains. 10、Phospholipids 磷脂Asymmetrical

lipid molecules with a hydrophilic head and

a hydrophobic tail. Lipids with a phosphate

group in place of one of the three fatty acid chains. Phospholipids are the

building blocks of cellular membranes.

Phospholipids have hydrophilic heads

(glycerol and phosphate) and hydrophobic tails (the non-polar fatty acids).

11、Hydrophilic 亲水的Water-loving.

Term applied to polar molecules that can form a hydrogen bond with water.

12、Hydrophobic 蔬水的

Water-fearing.Term applied to nonpolar

molecules that cannot bond with water.

Week 3

1.endocytosis 胞吞作用

The incorporation of materials from

outside the cell by the formation of

vesicles in the plasma membrane. The

vesicles surround the material so the

cell can engulf it.

2.phagocytosis 吞噬作用

A form of endocytosis in which white

blood cells surround and engulf

invading bacteria or viruses

3.exocytosis 胞吐作用

The process in which a

membrane-enclosed vesicle Trst fuses

with the plasma membrane and then opens

and releases its contents to the

outside

4.diffusion 扩散The

spontaneous movement of particles from

an area of higher concentration to an

area of lower concentration.

5.passive transport 被动运输Diffusion

across a plasma membrane in which the

cell expends no energy.

6.active transport 主动运输Transport of

molecules against a concentration

gradient (from regions of low

concentration to regions of high

concentration) with the aid of

proteins in the cell membrane and

energy from ATP.

Week4

1、C ell membrane 细胞膜 - the thin layer of

protein and fat that surrounds the cell.

The cell membrane is semipermeable, allowing some substances to pass into the cell and blocking others.

2、C ell wall–细胞壁 a thick, rigid

membrane that surrounds a plant cell. This layer of cellulose fiber gives the cell most of its support and structure. The cell wall also bonds with other cell walls to form the structure of the plant.

3、c hloroplast [–叶绿体 an elongated or

disc-shaped organelle containing chlorophyll. Photosynthesis (in which energy from sunlight is converted into chemical energy - food) takes place in the chloroplasts.

4、v acuole–液泡 a large,

membrane-bound space within a plant cell that is filled with fluid. Most plant cells

have a single vacuole that takes up much of the cell. It helps maintain the shape of the cell.

5、c hristae(线粒体嵴)- (singular crista [)

the multiply-folded inner membrane of a cell's mitochondrion that are finger-like projections. The walls of the cristae are the site of the cell's energy production (it is where ATP is generated).

6、g ranum叶绿体基粒- (plural grana) A stack

of thylakoid disks within the chloroplast is called a granum.

7、A myloplast [淀粉形成体- an organelle in

some plant cells that stores starch.

Amyloplasts are found in starchy plants like tubers and fruits.

8、A TP- ATP is short for adenosine

triphosphate; it is a high-energy molecule used for energy storage by organisms. In plant cells, ATP is produced in the cristae of mitochondria and chloroplasts.

9、c hlorophyll[叶绿素- chlorophyll is a

molecule that can use light energy from sunlight to turn water and carbon dioxide gas into sugar and oxygen (this process is called photosynthesis). Chlorophyll is magnesium based and is usually green.

10、Photosynthesis [ - a process in which

plants convert sunlight, water, and carbon dioxide into food energy (sugars and starches), oxygen and water. Chlorophyll or closely-related pigments (substances that color the plant) are essential to the photosynthetic process.

11、stroma[基质- part of the chloroplasts

in plant cells, located within the inner membrane of chloroplasts, between the grana. 12、thylakoid [ disk -类囊体(植物叶绿体

中的蛋白质和脂肪膜皮, 光合化学作用在此

进行thylakoid disks are disk-shaped

membrane structures in chloroplasts that

contain chlorophyll. Chloroplasts are

made up of stacks of thylakoid disks; a

stack of thylakoid disks is called a granum.

Photosynthesis (the production of ATP

molecules from sunlight) takes place on

thylakoid disks.

13、centrosome[–中心体 (also called the

"microtubule organizing center") a small

body located near the nucleus - it has a

dense center and radiating tubules. The

centrosomes is where microtubules are made.

During cell division (mitosis), the

centrosome divides and the two parts move

to opposite sides of the dividing cell. The

centriole is the dense center of the

centrosome.

14、cytoplasm[–细胞质the jellylike

material outside the cell nucleus in which

the organelles are located.

15、Golgi body–高尔基体 (also called

the Golgi apparatus or golgi complex) a

flattened, layered, sac-like organelle

that looks like a stack of pancakes and is

located near the nucleus. It produces the

membranes that surround the lysosomes. The

Golgi body packages proteins and

carbohydrates into membrane-bound

vesicles for "export" from the cell。16、lysosome [–溶酶体 (also called cell

vesicles) round organelles surrounded by

a membrane and containing digestive

enzymes. This is where the digestion of

cell nutrients takes place.

17、mitochondrion–线立体spherical to

rod-shaped organelles with a double

membrane. The inner membrane is infolded

many times, forming a series of projections (called cristae). The mitochondrion converts the energy stored in glucose into ATP (adenosine triphosphate) for the cell.

18、nuclear membrane–核膜 the membrane

that surrounds the nucleus.

19、nucleolus–核仁 an organelle within

the nucleus - it is where ribosomal RNA is produced. Some cells have more than one nucleolus.

20、nucleus–细胞核spherical body

containing many organelles, including the nucleolus. The nucleus controls many of the functions of the cell (by controlling protein synthesis) and contains DNA (in chromosomes). The nucleus is surrounded by the nuclear membrane.

21、ribosome核糖体- small organelles

composed of RNA-rich cytoplasmic granules that are sites of protein synthesis.

22、rough endoplasmic reticulum–粗面

质网(rough ER) a vast system of interconnected, membranous, infolded and convoluted sacks that are located in the cell's cytoplasm (the ER is continuous with the outer nuclear membrane). Rough ER is covered with ribosomes that give it a rough appearance. Rough ER transports materials through the cell and produces proteins in sacks called cisternae (which are sent to the Golgi body, or inserted into the cell membrane).

23、smooth endoplasmic reticulum–光面

质网(smooth ER) a vast system of interconnected, membranous, infolded and convoluted tubes that are located in the cell's cytoplasm (the ER is continuous with the outer nuclear membrane). The space within the ER is called the ER lumen.

Smooth ER transports materials through the

cell. It contains enzymes and produces and

digests lipids (fats) and membrane

proteins; smooth ER buds off from rough ER,

moving the newly-made proteins and lipids

to the Golgi body, lysosomes, and

membranes.

Week5

1.proteins 蛋白质Polymers made up of amino

acids that perform a wide variety of

cellular functions. One of the classes of organic macromolecules that function as

structural and control elements in living systems. Proteins are polymers of amino

acids linked together by peptide bonds. 2.peptide bond肽键A covalent bond that links

two amino acids together to form a

polypeptide chain. A covalent bond between the amine end of one amino acid and the acid end of another amino acid.

3. ribosomal RNA(核糖体RNA)One of the

three types of RNA; rRNA is a structural

component in ribosomes.

4.ribosomal subunits(核糖体亚单位)Two

units that combine with mRNA to form the

ribosomal-mRNA complex at which protein

synthesis occurs.

5.ribosomes [ m](核糖体)Small

organelles made of rRNA and protein in the cytoplasm of prokaryotic and eukaryotic

cells; aid in the production of proteins on the rough endoplasmic reticulum and

ribosome complexes. The site of protein

synthesis. The ribosome is composed of two subunits that attach to the mRNA at the

beginning of protein synthesis and detach when the polypeptide has been translated.

6.ribonucleic acid (RNA) Nucleic acid

containing ribose sugar and the base Uracil;

RNA functions in protein synthesis. The

single starnded molecule transcribed from one strand of the DNA. There are three types of RNA, each is involved in protein

synthesis. RNA is made up nucleotides

containing the sugar ribose, a phosphate

group, and one of four nitrogenous bases

(adenine, uracil, cytosine or guanine). 7.deoxyribonucleic acid (DNA) A nucleic

acid composed of two polynucleotide

strands wound around a central axis to form

a double helix; the repository of genetic

information. Nucleic acid that functions

as the physical carrier of inheritance for 99% of all species. The molecule is

double-stranded and composed of two

strands in an antiparallel and

complementary arrangement. The basic unit, the nucleotide, consists of a molecule of deoxyribose sugar, a phosphate group, and one of four nitrogenous bases.

8.nucleotides(核甘)The subunits of nucleic

acids; composed of a phosphate, a sugar,

and a nitrogen-containing base.The

fundamental structural unit of the nucleic acid group of organic macromolecules. Some nucleotides are involved in information

storage (as nucleotides in DNA), protein

synthesis (as nucleotides in RNA), and

energy transfers (as single nucleotide ATP, GTP, and double nucleotide NADH and NADPH).

9. nucleoid (类核)The area of the

prokaryotic cytoplasm where the chromatin is localized

10. nucleus (cell)(细胞核)The largest,

most prominent organelle in eukaryotic

cells; a round or oval body that is

surrounded by the nuclear envelope and

contains the genetic information necessary for control of cell structure and function.

11. translation (翻译)The synthesis of

protein on a template of messenger RNA;

consists of three steps: initiation,

elongation, and termination. Making of a

polypeptide sequence by translating the

genetic code of an mRNA molecule associated with a ribosome.

12.amino acid sequence(氨基酸序列)Also

known as the primary structure of a

protein/polypeptide; the sequence of amino acids in a protein/polypeptide controlled by the sequence of DNA bases. 13.primary structure(一级结构)The

sequence of amino acids in a protein 14.messenger RNA (mRNA) "Blueprint" for

protein synthesis that is transcribed from one strand of the DNA (gene) and which is translated at the ribosome into a

polypeptide sequence.

15.transfer RNAs (tRNAs) Small,

single-stranded RNA molecules that bind to amino acids and deliver them to the proper codon on messenger RNA. The trucks of

protein synthesis that carry the specified amino acid to the ribosome

16.genetic code(遗传密码)The linear

series of nucleotides, read as triplets, that speciTes the sequence of amino acids in proteins. Each triplet speciTes an

amino acid, and the same codons are used for the same amino acids in almost all

life-forms, an indication of the universal nature of the code.

17. codon(密码子)A sequence of three

nucleotides in messenger RNA that codes for

a single amino acid

18.anticodon(反密码子) A sequence of

three nucleotides on the transfer RNA

molecule that recognizes and pairs with a specific codon on a messenger RNA molecule;

helps control the sequence of amino acids in a growing polypeptide chain.

19. initiation codon (AUG)(起始密码子)

Three-base sequence on the messenger RNA that codes for the amino acid methionine;

the start command for protein synthesis.

20.methionine [蛋氨酸,甲硫氨酸

21.methionyl [甲硫氨酰(基)

22.Formyl [[化]甲酸基

23.aminoacyl [氨酰〔基

24.stop codon(终止密码子)The codon on a

messenger RNA molecule where protein

synthesis stops.

25.initiation(起始)The Trst step in

translation; occurs when a messenger RNA molecule, a ribosomal subunit, and a

transfer RNA molecule carrying the Trst

分子生物学词汇(S)

分子生物学词汇(S) 分子生物学词汇(S)分子生物学词汇(S)saccharomyces cerevisiae 酿酒酵母 saccharopine 酵母氨酸 saccluse [高尔基体]扁平膜囊 safranine 番红 safrole 黄樟素 sagamicin 相模霉素 saikoside 柴胡皂苷 sakaguchi reaction 坂口反应 salicin 水杨苷 salicylate 水杨酸盐 salinomycin 盐霉素 salmin 鲑精蛋白 salmonella 沙门氏菌属 salmonella typhi 伤寒(沙门氏)杆菌 saltatory conduction 跳跃传导 salvelin 鲑精蛋白 sapogenin 皂(角)苷配基,皂(角)苷元 saran wrap [商]saran包装膜,莎伦包装膜,偏氯伦包装膜,

[dow chemical公司商标,是用强韧而易弯曲的热敏塑料制成的包装用薄膜] sarcina 八叠球菌 sarcodina 肉足总纲 sarcomere 肌(原纤维)节 sarcomycin 肉瘤霉素 sarcoplasmic 肌质 sarcosinate 肌氨酸盐 sarcosine 肌氨酸 sarcosyl 十二烷基肌氨酸钠 sarcotubule 肌小管 sarin 萨林 satellite dna 卫星dna[真核细胞染色体dna经氯化铯密度梯度离心,其高度重复序列因组成不同而在主带旁自成一条或数条条带] satellitism 卫星现象[可特指一种微生物在另一种微生物菌落附近才能旺盛地生长繁殖的现象] satiety center 饱中枢 saturation hybridization 饱和杂交[一种组分大大过量而使另一组分的互补序列全部掺入双链中] saturation mutagenesis 饱和诱变[对基因的某一小区域内碱基进行多种形式的置换]

现代分子生物学_复习笔记完整版.doc

现代分子生物学 复习提纲 第一章绪论 第一节分子生物学的基本含义及主要研究内容 1 分子生物学Molecular Biology的基本含义 ?广义的分子生物学:以核酸和蛋白质等生物大分子的结构及其在遗传信息和细胞信息传递中的作用为研究 对象,从分子水平阐明生命现象和生物学规律。 ?狭义的分子生物学:偏重于核酸(基因)的分子生物学,主要研究基因或DNA的复制、转录、表达和调控 等过程,也涉及与这些过程相关的蛋白质和酶的结构与功能的研究。 1.1 分子生物学的三大原则 1) 构成生物大分子的单体是相同的 2) 生物遗传信息表达的中心法则相同 3) 生物大分子单体的排列(核苷酸、氨基酸)的不同 1.3 分子生物学的研究内容 ●DNA重组技术(基因工程) ●基因的表达调控 ●生物大分子的结构和功能研究(结构分子生物学) ●基因组、功能基因组与生物信息学研究 第二节分子生物学发展简史 1 准备和酝酿阶段 ?时间:19世纪后期到20世纪50年代初。 ?确定了生物遗传的物质基础是DNA。 DNA是遗传物质的证明实验一:肺炎双球菌转化实验 DNA是遗传物质的证明实验二:噬菌体感染大肠杆菌实验 RNA也是重要的遗传物质-----烟草花叶病毒的感染和繁殖过程 2 建立和发展阶段 ?1953年Watson和Crick的DNA双螺旋结构模型作为现代分子生物学诞生的里程碑。 ?主要进展包括: ?遗传信息传递中心法则的建立 3 发展阶段 ?基因工程技术作为新的里程碑,标志着人类深入认识生命本质并能动改造生命的新时期开始。 ? 第三节分子生物学与其他学科的关系 思考 ?证明DNA是遗传物质的实验有哪些? ?分子生物学的主要研究内容。 ?列举5~10位获诺贝尔奖的科学家,简要说明其贡献。

分子生物学名词解释全

1. 半保留复制(semiconservative replication):DNA复制时,以亲代DNA的每一股做模板,以碱基互补配对原则,合成完全相同的两个双链子代DNA,每个子代DNA中都含有一股亲代DNA链,这种现象称为半保留复制。 2.复制子replicon:由一个复制起始点构成的DNA复制单位。 57. 复制起始点(Ori C)DNA在复制时,需在特定的位点起始,这是一些具有特定核苷酸序列顺序的片段,即复制起始点。 24.(35)复制叉(replication fork)是DNA复制时在DNA链上通过解旋、解链和SSB蛋白的结合等过程形成的Y字型结构称为复制叉。 3. Klenow 片段klenow fragment:DNApol I(DNA聚合酶I)被酶蛋白切开得到的大片段。 4. 外显子exon、extron:真核细胞基因DNA中的编码序列,这部分可转录为RNA,并翻译成蛋白质,也称表达序列。 5.(56)核心启动子core promoter:指保证RNA聚合酶Ⅱ转录正常起始所必需的、最少的DNA序列,包括转录起始位点及转录起始位点上游TATA区。(Hogness区) 6. 转录(transcription):是在DNA的指导下的RNA聚合酶的催化下,按照硷基配对的原则,以四种核苷酸为原料合成一条与模板DNA互补的RNA 的过程。 7. 核酶(ribozyme):是具有催化功能的RNA分子,是生物催化剂,可降解特异的mRNA序列。 8.(59)信号肽signal peptide:常指新合成多肽链中用于指导蛋白质的跨膜转移(定位)的N-末端的氨基酸序列(有时不一定在N端)。 9.顺式作用元件(cis-acting element):真核生物DNA中与转录调控有关的核苷酸序列,包括增强子、沉默子等。 10.错配修复(mismatch repair,MMR):在含有错配碱基的DNA分子中,使正常核苷酸序列恢复的修复方式;主要用来纠正DNA双螺旋上错配的碱基对,还能修复一些因复制打滑而产生的小于4nt的核苷酸插入或缺失。修复的过程是:识别出正确的链,切除掉不正确的部分,然后通过DNA聚合酶III和DNA连接酶的作用,合成正确配对的双链DNA。 直接修复direct repair:是将被损伤碱基恢复到正常状态的修复。有三种修复方式:1光复活修复2、O6-甲基鸟嘌呤-DNA甲基转移酶修复3单链断裂修复。

分子生物学词汇(E1)

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ilamycin 岛霉素 ilarvirus 等轴不稳定环斑病毒组 illegitimate recombination 非常规重组 imago 成虫 imbibant 吸涨体 imbibition 吸涨(作用) imipramine 丙咪唑 immediate early gene 立即早期基因[有时特指病毒] immobiline [商]固定化电解质[pharmacia公司商标,为丙烯酰胺衍生物,带有官能团,可在凝胶上自动形成固定的ph梯度] immortalization 无限增殖化,永生化[使细胞长期不断维持增殖状态] immunoadhesin 免疫粘附素 immunoadjuvant 免疫佐剂 immunoadsorbent 免疫吸附剂 immunoadsorption 免疫吸附 immunoassay 免疫测定 immunobiology 免疫生物学 immunoblot 免疫印迹 immunoblotting 免疫印迹(法) immunocapture 免疫捕捉,免疫捕获 immunochemiluminescence 免疫化学发光

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分子生物学 第一章绪论 分子生物学研究内容有哪些方面? 1、结构分子生物学; 2、基因表达的调节与控制; 3、DNA重组技术及其应用; 4、结构基因组学、功能基因组学、生物信息学、系统生物学 第二章DNA and Chromosome 1、DNA的变性:在某些理化因素作用下,DNA双链解开成两条单链的过程。 2、DNA复性:变性DNA在适当条件下,分开的两条单链分子按照碱基互补原则重新恢复天然的双螺旋构象的现象。 3、Tm(熔链温度):DNA加热变性时,紫外吸收达到最大值的一半时的温度,即DNA分子内50%的双链结构被解开成单链分子时的温度) 4、退火:热变性的DNA经缓慢冷却后即可复性,称为退火 5、假基因:基因组中存在的一段与正常基因非常相似但不能表达的DNA序列。以Ψ来表示。 6、C值矛盾或C值悖论:C值的大小与生物的复杂度和进化的地位并不一致,称为C值矛盾或C值悖论(C-Value Paradox)。 7、转座:可移动因子介导的遗传物质的重排现象。 8、转座子:染色体、质粒或噬菌体上可以转移位置的遗传成分 9、DNA二级结构的特点:1)DNA分子是由两条相互平行的脱氧核苷酸长链盘绕而成;2)DNA分子中的脱氧核苷酸和磷酸交替连接,排在外侧,构成基本骨架,碱基排列在外侧;3)DNA分子表面有大沟和小沟;4)两条链间存在碱基互补,通过氢键连系,且A=T、G ≡ C(碱基互补原则);5)螺旋的螺距为3.4nm,直径为2nm,相邻两个碱基对之间的垂直距离为0.34nm,每圈螺旋包含10个碱基对;6)碱基平面与螺旋纵轴接近垂直,糖环平面接近平行 10、真核生物基因组结构:编码蛋白质或RNA的编码序列和非编码序列,包括编码区两侧的调控序列和编码序列间的间隔序列。 特点:1)真核基因组结构庞大哺乳类生物大于2X109bp;2)单顺反子(单顺反子:一个基因单独转录,一个基因一条mRNA,翻译成一条多肽链;)3)基因不连续性断裂基因(interrupted gene)、内含子(intron)、外显子(exon);4)非编码区较多,多于编码序列(9:1) 5)含有大量重复序列 11、Histon(组蛋白)特点:极端保守性、无组织特异性、氨基酸分布的不对称性、可修饰作用、富含Lys的H5 12、核小体组成:由组蛋白和200bp DNA组成 13、转座的机制:转座时发生的插入作用有一个普遍的特征,那就是受体分子中有一段很短的被称为靶序列的DNA会被复制,使插入的转座子位于两个重复的靶序列之间。 复制型转座:整个转座子被复制,所移动和转位的仅为原转座子的拷贝。 非复制型转座:原始转座子作为一个可移动的实体直接被移位。 第三章DNA Replication and repair 1、半保留复制:DNA生物合成时,母链DNA解开为两股单链,各自作为模板(template)按碱

分子生物学名词解释

1, 错义突变:DNA分子中碱基对的取代,使得mRNA的某一密码子发生变化,由它所编码 的氨基酸就变成另一种的氨基酸,使得多肽链中的氨基酸顺序也相应的发生改变的突变. 2 无义突变:由于碱基对的取代,使原来可以翻译某种氨基酸的密码子变成了终止密码子的突变. 3 同义突变:碱基对的取代并不都是引起错义突变和翻译终止,有时虽然有碱基被取代,但 在蛋白质水平上没有引起变化,氨基酸没有被取代,这是因为突变后的密码子和原来的密码子代表同一个氨基酸的突变. 4移码突变:在编码序列中,单个碱基,数个碱基的缺失或插入以及片段的缺失或插入等均 可以使突变位点之后的三联体密码阅读框发生改变,不能编码原来的蛋白质的突变. 1转化:指质粒DNA或以它为载体构建的重组DNA导入细菌的过程. 2 感染:以噬菌体,粘性质粒和真核细胞病毒为载体的重组DNA分子,在体外经过包装成 具有感染能力的病毒或噬菌体颗粒,才能感染适当的细胞,并在细胞内扩增. 3转导:指以噬菌体为载体,在细菌之间转移DNA的过程,有时也指在真核细胞之间通过 逆转录病毒转移和获得细胞DNA的过程. 4转染:指病毒或以它为载体构建的重组子导入真核细胞的过程. 5.癌基因:是细胞内控制细胞生长的基因,具有潜在的诱导细胞恶性转化的特性.当癌基因 结构或表达发生异常时,其产物可使细胞无限制增殖,导致肿瘤的发生.包括病毒癌基因和细胞癌基因. 6., 细胞癌基因:存在于正常的细胞基因组中,与病毒癌基因有同源序列,具有促进正常细胞 生长,增殖,分化和发育等生理功能.在正常细胞内未激活的细胞癌基因叫原癌基因,当其受到某些条件激活时,结构和表达发生异常,能使细胞发生恶性转化. 7. 病毒癌基因:存在于病毒(大多是逆转录病毒)基因组中能使靶细胞发生恶性转化的基因. 它不编码病毒结构成分,对病毒无复制作用,但是当受到外界的条件激活时可产生诱导肿瘤发生的作用. 8. 基因诊断:以DNA或RNA为诊断材料,通过检查基因的存在,结构缺陷或表达异常, 对人体的状态和疾病作出诊断的方法和过程. 9 RFLP:即限制性片段长度多态性,个体之间DNA的核苷酸序列存在差异,称为DNA多 态性.若因此而改变了限制性内切酶的酶切位点则可导致相应的限制性片段的长度和数量发生变化,称为RFLP. 10 基因治疗:一般是指将限定的遗传物质转入患者特定的靶细胞,以最终达到预防或改变特殊疾病状态为目的治疗方法. 11, 反义RNA:碱基序列正好与有意义的mRNA互补的RNA称为反义RNA.可以作为一种 调控特定基因表达的手段. 12, 核酶:是一种可以催化RNA切割和RNA剪接反应的由RNA组成的酶,可以作为基因表 达和病毒复制的抑制剂. SSCP:单链构象多态性检测是一种基于DNA构象差别来检测点突变的方法.相同长度的 单链DNA,如果碱基序列不同,形成的构象就不同,这样就形成了单链构象多态性. 13, 管家基因:在生物体生命的全过程都是必须的,且在一个生物个体的几乎所有细胞中持续表达的基因. 14, 细胞全能性:指同一种生物的所有细胞都含有相同的DNA,即基因的数目和种类是一样的,但在不同阶段,同一个体的不同组织和器官中基因表达的种类和数目是不同的. 15, SD序列:转录出的mRNA要进入核糖体上进行翻译,需要一段富含嘌呤的核苷酸序列与

(完整版)医学专业英语

cardiovascular diseases; 脑垂体的功能the function of pituitary; 泌尿道urinary tract; 分子molecule; 动脉artery; 内分泌学endocrinology; 呼吸困难dyspnea; 唾液saliva; 组织学histology; 血液循环blood circulation; 血液学hematology; 生理学physiology; 解剖学anatomy; 女性生殖系统femal reproductive system; 神经细胞nerve cell; 免疫学immunology; 消化不良dyspepsia; 随意肌voluntary muscle; 胚胎学embryology; 心理学psychology; 细胞学cytology; 原生质protoplasm; 细胞膜cell membrane; 细胞核nucleus; 细胞质(浆)cytoplasm; 脱氧核糖核酸deoxyribonucleic acid; 能半渗透的semipermeable; 分子生物学molecular biology; 碳水化合物carbohydrate; 有区别性的differentially; 使…完整intact; 根据according to; 遗传特性hereditary trait; 渗滤diffusion; 转换transaction; 蓝图blueprint; 染色体chromosome; 色素pigment; 排出废液excrete waste fluid; 散开disperse; 脉冲信号impulse; 核糖核酸ribonucleic acid; 损害正常功能impair the normal function; 污染环境pollute environment; 功能失调malfunction; 致病因子causative agents; 易受侵害的人群vulnerable groups; 局部化的感染localized infection; 花柳病venereal disease; 抗原与抗体antigen&antibody; 肌电图electromyogram; 多发性硬化multiple sclerosis; 心电图electrocardiograph; 疾病的后遗症sequelea of disease; 光纤技术fiber optic technology; 造血系统hematopoietic system; 致命的疾病fatal disease; 体液body fluid; 无副作用的治疗hazard-free treatment; 无侵犯的实验检查non-invasive laboratory test; 核磁共振nuclear magnetic resonance; 葡萄糖耐糖实验the glucose-tolerance test; 乐观的预后optimistic prognosis; 超声波检测法ultrasonography; 病史medical history; 随访活动follow-up visit; 营养不良nutritional deficiency; 使细节显著highlight detail; 脑电图electroencephalogram; 缺血的组织blood-starved tissue; 肌纤维muscle fiber; 随意肌voluntary muscle; 消化道alimentary canal; 肌腹fleshy belly of muscle; 横纹肌striated muscle; 肌肉痉挛cramps of muscle; 肌肉收缩muscle contraction; 肌肉附着点attachment of the muscle; 肌肉放松relaxation of muscle; 动脉出血arterial hemorrhage; 止端insertion;起端origion;供血blood supply; 屈肌flexor; 蛋白分子protein molecule; 纤维结缔组织fibrous connective tissue; 伸肌extensor; 意志力willpower; 横切面transverse section; 起搏器pacemaker; 肌萎缩muscle atrophy; 重症肌无力myasthenia gravis; 弥散性局部缺血diffuse ischemia; 常染色体隐性autosomal recessive; 全身性感染systemic infection; 受累的肌肉muscle involved; 显著相关性significant correlation; 神经末梢nerve terminal; 自体免疫反应autoimmune reaction; 神经支配innervation; 肌营养不良muscular dystrophy; 慢性营养不良chronic mulnutrition; 先天性肌病congenital myopathy; 预期寿命life expectancy; 免疫紊乱immunologic derangemant; 发病高峰年龄the peak age of onset; 胸腺肿瘤thymoma; 呼吸肌受累the involvement of respiratory muscle; 感染性肌炎inflammatory myositic; 去神经支配denervation; 矿物质吸收mineral absorption; 机械应力mechanical stress; 骨基质有机部分the organic parts of bone matrix; 青春期早熟premature puberty; 蛋白溶解酶protein-digesting enzyme; 破骨细胞osteoclast; 松质骨spongy bone; 骨折fracture; 不规则骨irregular bone; 骨骼系统skeletal system; 维生素吸收vitamin absorption; 骨钙丧失the loss of calcium from bone; 生长激素growth hormone;

分子生物学与基因工程复习资料

分子生物学与基因工程 绪论 1、分子生物学与基因工程的含义 从狭义上讲,分子生物学主要是研究生物体主要遗传物质-基因或DNA的结构及其复制、转录、表达和调节控制等过程的科学。 基因工程是一项将生物的某个基因通过载体运送到另一种生物的活体细胞中,并使之无性繁殖和行使正常功能,从而创造生物新品种或新物种的遗传学技术。 2、分子生物学与基因工程的发展简史,特别是里程碑事件,要求掌握其必要的理由 上个世纪50年代,Watson和Crick提出了的DNA双螺旋模型; 60年代,法国科学家Jacob和Monod提出了的乳糖操纵子模型; 70年代,Berg首先发现了DNA连接酶,并构建了世界上第一个重组DNA分子; 80年代,Mullis发明了聚合酶链式反应(Polymerase Chain Reaction,PCR)技术; 90年代,开展了“人类基因组计划”和模式生物的基因组测序,分子生物学进入“基因组时代” 3、分子生物学与基因工程的专业地位与作用。 核酸概述 1、核酸的化学组成 2、核酸的种类与特点:DNA和RNA的区别 (1)DNA含的糖分子是脱氧核糖,RNA含的是核糖;

(2)DNA含有的碱基是腺嘌呤(A)、胞嘧啶(C)、鸟嘌呤(G)和胸腺嘧啶(T),RNA含有的碱基前3个与DNA完全相同,只有最后一个胸腺嘧啶被尿嘧啶(U)所代替; (3)DNA通常是双链,而RNA主要为单链; (4)DNA的分子链一般较长,而RNA分子链较短。 3、DNA作为遗传物质的直接和间接证据; 间接: (1)一种生物不同组织的细胞,不论年龄大小,功能如何,它的DNA含量是恒定的,而生殖细胞精子的DNA含量则刚好是体细胞的一半。多倍体生物细胞的DNA含量是按其染色体倍数性的增加而递增的,但细胞核里的蛋白质并没有相似的分布规律。 (2)DNA在代谢上较稳定。 (3)DNA是所有生物的染色体所共有的,而某些生物的染色体上则没有蛋白质。(4)DNA通常只存在于细胞核染色体上,但某些能自体复制的细胞器,如线粒体、叶绿体有其自己的DNA。 (5)在各类生物中能引起DNA结构改变的化学物质都可引起基因突变。 直接:肺炎链球菌试验、噬菌体侵染实验 4、DNA的变性与复性:两者的含义与特点及应用 变性:它是指当双螺旋DNA加热至生理温度以上(接近100oC)时,它就失去生理活性。这时DNA双股链间的氢键断裂,最后双股链完全分开并成为无规则线团的过程。简而言之,就是DNA从双链变成单链的过程。

分子生物学名词解释

Central dogma (中心法则):DNA 的遗传信息经RNA 一旦进入蛋白质就不能再输出了。Reductionism (还原论):把问题分解为各个部分,然后再按逻辑顺序进行安排的研究方法。Genome (基因组):单倍体细胞的全部基因。 transcriptome(转录组):一个细胞、组织或有机体在特定条件下的一组完整基因。roteome (蛋白质组):在大规模水平上研究蛋白质特征,获得蛋白质水平上的关于疾病的发生、细胞代谢等过程的整体而全面的认识。 Metabolome (代谢组):对生物体内所有代谢物进行定量分析并寻找代谢物与生病理变化的相关关系的研究方法。 Gene (基因):具有遗传效应的DNA 片段。 Epigenetics (表观遗传学现象):DNA 结构上完全相同的基因,由于处于不同染色体状态下具有不同的表达方式,进而表现出不同的表型。 Cistron (顺反子):即结构基因,决定一条多肽链合成的功能单位。 Muton(突变子):顺反子中又若干个突变单位,最小的突变单位被称为突变子。 recon(交换子):意同突变子。 Z DNA(Z型DNA) :DNA 的一种二级结构,由两条核苷酸链反相平行左手螺旋形成。Denaturation (变性):物质的自然或非自然改变。 Renaturation (复性):变形的生物大分子恢复成具有生物活性的天然构想的现象。egative superhelix (负超螺旋):B-DNA 分子被施加左旋外力,使双螺旋体局部趋向松弛,DNA分子会出现向右旋转的力的超螺旋结构。 C value paradox (C值矛盾):生物 overlapping gene(重叠基因):不同的基因公用一段相同的DNA序列。体的大C值与小c值不相等且相差非常大。 interrupted gene (断裂基因):由若干编码区和非编码区连续镶嵌而成的基因。 splitting gene(间隔基因):意思与断裂基因相同。 jumping gene(跳跃基因):一段可以从原位上单独复制并断裂下来,环化后插入另一位点并对其后的基因起调控作用。 Transposon (转座子):与跳跃基因意思相同。 eudo gene(假基因):与功能基因相似却失去基因活性的基因。 Retro-transposon(反转录转座子):转座子从DNA到RNA再到DNA的转移过程。Replicon (复制子):从复制起点到复制终点的DNA区段。 emiconservative replication(半保留复制):DNA复制过程中亲代DNA双链分开作为模板合成两条新生子链,每条新生链均含有一条母链和一条新合成的链。 emi-discontinuous replication(半不连续复制):前导链以连续复制的方式完成子代DNA的合成,而后随链以不连续复制的方式完成冈崎片段的合成。 leading strand(前导链):随着复制叉的分开,以显露的单链DNA为模板聚合dNTP而延伸的链。 lagging strand (后随链):复制叉的延伸与新生链的延伸背道而驰的链。 dUMP fragment (dUMP片段):约1200个核苷酸中有一个错配而引起的DNA 链被切断而形成的大小形似冈崎片段的DNA 分子片段。 replisome (复制体):连接酶等内在的酶分子集中于复制叉处组成一个复合体协同互作,完成DNA 复制的复合体。 Telomerase (端粒酶):端粒酶是参与真核生物染色体末端的端粒DNA 复制的一种核糖核蛋白酶。由RNA 和蛋白质组成,其本质是一种逆转录酶。它以自身的RNA 作为端粒DNA 复制的模版,合成出富含脱氧单磷酸鸟苷Deoxyguanosine Monophosphate(dGMP)

(完整版)医学专业英语.doc

英核磁共振 nuclear magnetic resonance; 心血管疾病 cardiovascular diseases; 葡萄糖耐糖 the glucose-tolerance test; 垂体的功能 the function of pituitary; 的后optimistic prognosis; 泌尿道 urinary tract; 超声波法ultrasonography; 分子 molecule; 病史 medical history; 脉 artery; 随活 follow-up visit; 内分泌学 endocrinology; 养不良 nutritional deficiency; 呼吸困 dyspnea; 使著highlight detail; 唾液 saliva; electroencephalogram; 学 histology; 缺血的blood-starved tissue; 血液循 blood circulation; 肌 muscle fiber; 血液学 hematology; 随意肌 voluntary muscle; 生理学 physiology; 消化道 alimentary canal; 解剖学 anatomy; 肌腹 fleshy belly of muscle; 女性生殖系femal reproductive system; 横肌 striated muscle; 神胞 nerve cell; 肌肉 cramps of muscle; 免疫学 immunology; 肌肉收 muscle contraction; 消化不良 dyspepsia; 肌肉附着点 attachment of the muscle; 随意肌 voluntary muscle; 肌肉放松 relaxation of muscle; 胚胎学 embryology; 脉出血 arterial hemorrhage; 心理学 psychology; 止端 insertion; 起端 origion; 供血 blood supply; 胞学 cytology; 屈肌 flexor; 原生 protoplasm; 蛋白分子 protein molecule; 胞膜 cell membrane; fibrous connective tissue; 胞核 nucleus; 伸肌 extensor; 胞 ( )cytoplasm; 意志力 willpower; 脱氧核糖核酸deoxyribonucleic acid; 横切面 transverse section; 能半渗透的semipermeable; 起搏器 pacemaker; 分子生物学molecular biology; 肌萎 muscle atrophy; 碳水化合物carbohydrate; 重症肌无力myasthenia gravis; 有区性的differentially; 弥散性局部缺血 diffuse ischemia; 使?完整 intact; 常染色体性autosomal recessive; 根据 according to; 全身性感染systemic infection; 特性 hereditary trait; 受累的肌肉muscle involved; 渗 diffusion; 著相关性significant correlation; transaction; 神末梢 nerve terminal; blueprint; 自体免疫反autoimmune reaction; 染色体 chromosome; 神支配 innervation; 色素 pigment; 肌养不良muscular dystrophy; 排出液 excrete waste fluid; 慢性养不良chronic mulnutrition; 散开 disperse; 先天性肌病congenital myopathy; 脉冲信号 impulse; 期寿命 life expectancy; 核糖核酸 ribonucleic acid; 免疫紊乱 immunologic derangemant; 害正常功能 impair the normal function; 病高峰年 the peak age of onset; 染境 pollute environment; 胸腺瘤 thymoma; 功能失 malfunction; 呼吸肌受累 the involvement of respiratory muscle; 致病因子 causative agents; 感染性肌炎inflammatory myositic; 易受侵害的人群vulnerable groups; 去神支配denervation; 局部化的感染localized infection; 物吸收mineral absorption; 花柳病 venereal disease; 机械力 mechanical stress; 抗原与抗体antigen&antibody; 骨基有机部分 the organic parts of bone matrix; 肌 electromyogram; 青春期早熟premature puberty; 多性硬化multiple sclerosis; 蛋白溶解protein-digesting enzyme; 心 electrocardiograph; 破骨胞 osteoclast; 疾病的后症sequelea of disease; 松骨 spongy bone; 光技 fiber optic technology; 骨折 fracture; 造血系 hematopoietic system; 不骨 irregular bone; 致命的疾病fatal disease; 骨骼系 skeletal system; 体液 body fluid; 生素吸收vitamin absorption; 无副作用的治hazard-free treatment; 骨失 the loss of calcium from bone; 无侵犯的non-invasive laboratory test; 生激素 growth hormone;

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