专业英语读书报告(许洁)
英语专业书籍读书笔记800字

英语专业书籍读书笔记800字Tonight I watched the movie for the third time. I really enjoy this film so much.So what's your choice between being a common person or a hero with people's reects? Most people will choose the latter. But what will be your choice if the cost is laying your lover among the risks? What will it be if the cost is you can never tell the girl, who you love so much, that you love her? The ider man had this contradiction. But finally he still chose the latter, not in order to be a hero, but to make this word peaceful.I was so moved by the words the Aunt Mary said:You will never guess what he wants to be, the ider man. He knows the hero when he sees one, too a few characters out there, flying all around out there, saving old girls like me. Lord knows kids like Henry need hero courageous, sacrificing for people, setting examples for all of us. Everybody loves a hero. People enthrone them, cheer them, scream their names and years later they will tell how they stood in the rain for hours just to get a glimpse the one who taught them to hold on to stand longer. I believe there is a hero in all of us. They keep us be honest, giveus strength, make us noble, and finally allow us to die with proud. Ever though sometimes we have to initiatively give up the thing we want most, even our dreams. Spider man did that for Henry, so he wants to know where he is gone. He needs him.The ider man got much from these words, so did I. And what about you? what's the hero lying in you?。
英语读书报告20篇

英语读书报告20篇英语读书报告20篇英语读书报告〔一〕:《小王子》英语读书报告I have read some books this term。
The one which impressed me most is 。
I brought this book last term。
The book consists of three versions: French,Chinese and English。
I read Chinese version first,which made my second read in English much easier。
Im not going to talk about the fairy tales writing skills,writing style and what philosophy it contains。
Instead,I will pick out several sentences which moved me a lot and then show my feeling。
In part 4,there is "Not everyone has had a friend。
' And later,in part 17,theres "Its also lonely with people。
' The two sentences nearly have the same meaning。
A true friend is of a lifetime。
You may say that: "I have a lot of friends,and we always play together and chat together' But in fact,friends are not those whom you always stay with。
【参考文档】英语读书报告500字(共7篇word版本 (11页)

本文部分内容来自网络整理,本司不为其真实性负责,如有异议或侵权请及时联系,本司将立即删除!== 本文为word格式,下载后可方便编辑和修改! ==英语读书报告500字(共7篇篇一:英语读书报告华南理工大学广州学院英语专业学号班级批阅老师(201X级)读书报告201X30097023 姓名英语(2)班完稿日期成绩黄玉珍 201X年 5月 30日Love or Money ? I. Bibliographical information1. Type of materials you selected to read: a book written in EnglishB. an article written in English)2. Topical area:B. Linguistics C. Translation D. Education E. Trade F. Miscellaneous)3. If you read a book, provide its bibliographical information below: Title:Author(s):Love or Money? Edition:Oxford Univ Pr Year of publication:Place:Publisher:II. Information about your readingThe reasons why you selected the book or the article: (≥20 words) At first I thought that this story was love story which was about the love and the money between men and women, but it wasn't. I like mystery stories, so this book1. The amount of time you spent reading the book or the article: Two weeks2. If you finish reading the entire book or article, SIGN your name here: 黄玉珍3. If you did not finish reading the book, how many pages did you read? __64___ ( __64__ pages in total)III. A brief summary of the book or the article (≥150 words)In Clarkson family, everyone are thirsty for the big fortune of the host——Molly. What’s more, all of them expect that she can die early, so they can get her house, her big garden and her amazing heritage. They all need money to make their lives more luxurious and more comfortable. As a matter of fact, none of them love Molly. Atone family party, her two daughters, her son, and her brother-in-law came together. They forced Molly to give them money by saying some words. Molly was going mad, so she came upstairs. Next day, unlucky thing happened. When her little daughter Diane came to her mother’s house, she found her mother dead on the bed. She shouted. And thenthe police found a bottle of sleeping pills in milk. Finally, they proved she died of an overdose of pills, but committed suicide. Therefore, the police began to make a survey. After dinner, everybody paid respect to Molly before sleeping. And they asked for her help in money again. They were hypocritical. And Molly refused their requests. At last, the truth surfaced. To my surprise, the murder was her elderdaughter——Jackie. And the only reason was that her mother didn’t agree with her marriage, and she was dissatisfied. In the end, theygo the money, Albert, Roger, Diane. They are all rich now.( 225 words)IV. Comments on the book or the article1. What are the strengths and weaknesses of the book or the article? (≥50words)The strengths are easy to read and understand. The vocabularies inthat book are simple. Also it reveals the attitude between money and family relationship. Led most of us think about the important thingsthat we should treasure. The weaknesses are too short and lack of more details.2. What is the part of this book or article that had the greatest impact onyou? Why? (≥50 words)Molly killed her husband in a car accident, so she got all of the money. And then, she refused her elder daughter’s marriage because she didn’t want to lose her garden. Last, she never saw her sister who was going to die, and she didn’t want to pay for me dical fee of her sister. Those parts of book that hadthe greatest impact on me.Because I think in Molly’s heart, she also thought money was themost important thing in her life. She was so closefisted to her relative. I think she deserved this death.3. Is the writing effective, powerful, difficult, or beautiful? (≥50 words) The writing is effective and powerful. Firstly, it relates to real life. It describes the story in a birthday party. Secondly, it attracts readers. Molly was killed by people. The detective found the murder at last. It was Molly’s elder daughter Jackie. To the readers, they can’t believe it. She hooted her mother because she gave her nothing and marry her lover.4. What new facts did you learn that you hadn’t known before? (≥50words)Nowadays, more and more people become really, they can do anythingfor money. With money, they think they can get anything what they want, even true love. But they don’t know they are truly wrong. How mournful they are! How mournful the world it is. We should treasure the true love instead of money.5. If you could talk to the author, what would you say? (≥50 words) Thank you for you literary work which makes me understand that there was no need to think about money importantly. No matter how yourmoth er treat you, she must love you.. So don’t miss our family. So please be happy with our family. Because family relation is more valuable than money.6. What did you learn about yourself as a result of reading this book orarticle? (≥50 words)the love of our parents, we need the love of our friends, we need the love of the society. Love is just like a plant, if you plant it, it will come out a beautiful flower, which can be wealth, happiness or health. Money is a useful thing, but it is not the most important if you seize the love. I think money will come behind it.7. Note down three useful expressions you learned from your reading and make one sentence for each expression.1) [Useful expression] She wanted her mother to be happy this evening,but it was very difficult.[Sentence you made] Marry wanted to win the scholarship, but it was very difficult.2)[Useful expression] On the little table next to the bed was a hot cup of coffee and an empty cup.[Sentence you made] On the little table next to the bed was biographical notes.3)[Useful expression] He was the family doctor and he knew all the Clarkson family very well.[Sentence you made] Apparently, people in America, Canada, and Singapore are very happy; people living in India and Russia, not surprisingly, are not happy.7. General remarks: (≥200 words)can’t buy happiness, but the old saying seems to be supported by book. Many people still cling to the belief that gaining riches will be the answer to all their problems, yet they are probably mistaken.If material wealth does not bring happiness, then what does?Perhaps happiness has something to do with where you live. Family relationships in particular seem to be the key to long-term contentment. Actually, falling in love and having children are two of the situations that bring the greatest happiness.People increasingly spend more time alone watching TV or surfing the Internet rather than spending time with family. I think time spent with your family is a better investment than time spent making. Above all, family always is our harbor.word篇二:飘的读书报告英文版(共6篇)篇一:飘英文读书报告tomorrow is a new dayreading on gone with the windanother day.gone with the wind has a very significant position in our society. we feel america is an incredible and strange country, but gone with the wind uncovers her too softhearted veil, making people see many things that dirty and glorious are coexisted. it also has a special significance to adolescents. this novel became famous overnight as soon as it published. this novel which reached a length of 1000 pages shocked american. the movie gone with the wind was adapted from this novel. the movie made the novel even more famous. it is quite worth reading.篇二:飘--读书报告(英文)book reportgone with the wind姓名:学号:班级:教授:bookreporttitle: gone with the windauthor: margaret mitchellgee: novelpublish place and time: aviation industry press first edition,fourth printing summary:gone with the wind was published in the usa in 1936 and was the only work of margaret mitchell in her life. margaret mitchell spent almost ten years finishing this works ,though she could not accept the reputation before she went to the heaven later, she won pulitzerprize in 1937 by gone with the wind?gone with the wind takes place in the southern united states in the state of georgia during the american civil war and the reconstruction era that followed the war. the novels unfolds against the backgroundof rebellion in southern states, georgia among them, have declared their secession from the united states and formed the confederate states of america, after abraham lincoln was elected president withno ballots from ten southern states where slavery was legal. adispute over states rights has arisen involving enslaved african people who were the source of manual labor on cotton plantations throughout the south. the story opens in april 1861 at the tara plantation, which is owned by a wealthy irish immigrant family, the oharas. gone with the wind was on the background that lincoln advocated emancipation of the black slaves, describing the life of scarlett ohara. when scarlett was 16,she loved ashley who was aboutto marry her cousin—melanie. after being suffered the refuse of ashley, scarlett married charlie quickly in order to revenge ashley and she set the date of her wedding for the day before ashley’s, in fact, ashley married on the first of may. but scarlett became a widow soon because of the civil war. the south was always retreating in defeat, and scarlett took melanie and melanie’s childto leave the dangerous city. facing the ruined tara, his abnormal father and her died mother; scarlett did not know what to do and howto do. after 5 years’ battle, the north defeated the south, at this time ashley following with the armyfrom battle came back to reunion with his wife and his son .they lost their house in the battle, so ashley with melanie and their son hadto stay at tara and lived a tough life with scarlett. to our surprise, scarlett married frank—her young sister’s boyfriend. just for money, she married frank. unfortunately, frank was also died soon aftertheir marriage. scarlett lost her arrogance and became easygoing. facing rhett’s propose marriage, she answered without hesitation. they had a beautiful and lovely girl; they put all of their love totheir honey. although they had some unhappy trivial things, theystill lived with each other. the misfortunate covered scarlett again, their daughter died from riding a horse. rhett suck into the sad gloom, he thought scarlett did not love him and she loved ashley as usual. at the same time, his daughter also left him, in this so-called family he found no love; he could not stay any minute, even a second! there was no reason for him to live with scarlett who did not love him, so he left scarlett and came back to his house in which he once lived. he just wanted to live a simple but happy life. scarlett found who she really loved was rhett, so she wanted to let rhett know the truth that she loved rhett so many years just she did not know, but she was not given the chance. scarlett seated herself in the house praying tomorrow is another day.review:篇三:飘读书报告安同巧 201X14540311 12英语3班tomorrow is a new day------reading on gone with the windgone with the wind has a very significant position in our society. we feel america is an incredible and strange country, but gone with the wind uncovers her too softhearted veil, making people see many things that dirty and glorious are coexisted. it also has a special significance to adolescents. this novel became famous overnight as soon as it published. this novel which reached a length of 1000 pages shocked american. the movie gone with the wind was adapted from this novel. the movie made the novel even more famous. it is quite worth reading.篇四:《飘》读书报告襄樊学院计算机科学与技术专业王伟《飘》读书报告作者简介:美国女作家玛格丽特·米切尔,自1936年出版之日起,《飘》这部美国内战时期的罗曼史便打破了当时所有的出版记录。
英语读书报告(优秀7篇)

英语读书报告(优秀7篇)英语读书报告篇一Jane Austen's novel Pride and Prejudice is a lighthearted tale of love and marriage in eighteenth-century England. It centers on the elder sisters of the Bennet family, Jane and Elizabeth. Their personalities, misunderstandings and the roles of pride and prejudice play a lavishing story. This story is told from third point of view. From my perspective Jane Austen wanted to convey love wins over prejudice and to not just take in the saying of first impression but to look in the person's character deeper. Jane Austin was born in 1775 in Stevenson, Hampshire. Her family wasn't rich but managed to give her a decent education. She was the seventh of eight children of her father, a clergyman. Like other young women of her social class, Jane and her sister Cassandra were educated mostly at home in subjects of music, drawing, painting, needlework, and social behavior. Her father's encouragement and her own enjoyment in reading led Jane to became very well read. At fourteen she began to write little plays for home theatricals. She also wrote nonsense story's to entertain her family. Jane would spend the majority of the evening in the corner of the room with her manuscript and blotter observing the world surrounding her. She would write when the room was quiet and if she were interrupted, she would cover her manuscript with a blotter and continued when the room was silent again. Before Jane Austen died of cancer in 1817 at Winchester, she had already published six successful novels: Sense and Sensibility, Pride and Prejudice, Northanger Abbey, Mansfield Park, Emma, and Persuasion, respectively. Jane Austen is now buried in the ancient cathedral in Winchester of her native Hampshire. Pride and Prejudice lets you travel through lives back then but also she portrays the lives of modern days. You can read it and you might be able to recall some parts of the book as your life, but in a different time period如何写英语读书报告篇二技术部新员工工作总结来到公司已经有一个多月了,在公司的点点滴滴都让我受益匪浅!不管是在原画、动画还是软件的运用上都让我学到了很多的知识!这些知识可能是我在人生中重要的一笔!作为公司新人,在工作实践中我学到了很多知识。
大学生英文读书报告范文800字

大学生英文读书报告范文800字Title: College Student English Reading ReportIntroductionAs college students, reading is an essential part of our academic and personal development. Reading not only helps us acquire knowledge but also improves our language skills. In this report, I will discuss my reading habits, the books I have read recently, and the benefits I have gained from reading in English.Reading HabitsI have always been an avid reader since childhood. In college, I make sure to set aside time for reading every day, even if it is just for a few minutes. I prefer to read in the morning or before going to bed as it helps me relax and focus. I also like to read in different places like the library, coffee shops, or even outdoors.Books I Have Read Recently1. "To Kill a Mockingbird" by Harper Lee - This classic novel explores themes of racism and injustice in the American South. I was deeply moved by the story and the characters, and it made me reflect on the importance of empathy and courage.2. "The Great Gatsby" by F. Scott Fitzgerald - This novel set in the Jazz Age follows the life of Jay Gatsby and explores themes of wealth, love, and the American Dream. The beautifully written prose and compelling plot captivated me from start to finish.3. "1984" by George Orwell - This dystopian novel depicts a totalitarian society where individual freedom is restricted and Big Brother watches over everyone. The book's themes of surveillance and propaganda are still relevant today and made me think about the power of government control.Benefits of Reading in EnglishReading in English has greatly improved my language skills, especially vocabulary and grammar. I have learned new words and phrases that have enhanced my writing and communication skills. Reading also exposes me to different writing styles and genres, helping me become a more versatile reader.Furthermore, reading in English has broadened my perspective and understanding of different cultures and worldviews. I have gained insights into history, society, and human behavior through the books I have read. Reading has also sparked my curiosity and made me more open-minded towards diverse ideas and opinions.ConclusionIn conclusion, reading in English is a valuable habit that has enriched my college experience and personal growth. It has expanded my knowledge, improved my language skills, and broadened my worldview. I will continue to read widely and engage with different authors and genres to further enhance my reading proficiency and intellectual development.。
小学英语教师专业书籍读后感

小学英语教师专业书籍读后感As an aspiring primary school English teacher, I have had the privilege of delving into a wealth of professional literature that has greatly enriched my understanding of the field. In this essay, I will share my reflections on some of the key insights and takeaways I have gained from these invaluable resources.One of the most impactful books I have read is "Effective Strategies for Teaching English Language Learners" by Debbie Zacarian. This comprehensive guide has provided me with a deeper appreciation for the unique challenges and needs of students from diverse linguistic backgrounds. The author emphasizes the importance of creating a culturally responsive classroom environment that celebrates the linguistic and cultural diversity of the students. She offers practical strategies for differentiated instruction, scaffolding, and the incorporation of students' native languages to facilitate their English language acquisition.Another standout work is "The Essentials of English Language Arts Teaching" edited by Diane Lapp and Douglas Fisher. This anthologycovers a wide range of topics, from the development of foundational literacy skills to the integration of technology in the classroom. One chapter that particularly resonated with me was the one on fostering a love of reading. The authors stress the significance of creating a reading-rich environment, where students are exposed to a diverse array of high-quality literature and encouraged to engage in meaningful discussions and activities surrounding the texts. They also provide guidance on how to select appropriate texts and implement effective read-aloud practices.In addition to these broad-based resources, I have also delved into more specialized works that have helped me refine my understanding of specific aspects of English language instruction. For instance, "Teaching Grammar in Context" by Constance Weaver has been instrumental in shaping my approach to grammar instruction. The author challenges the traditional, prescriptive approach to grammar teaching and instead advocates for a more contextual, functional understanding of language structures. She emphasizes the importance of teaching grammar in the context of authentic, meaningful communication, rather than as isolated rules and exercises.Another valuable resource I have encountered is "Differentiated Literacy Instruction for English Language Learners" by Suzanne Irujo. This book has deepened my appreciation for the need to tailorliteracy instruction to the diverse needs and learning styles of English language learners. Irujo provides a wealth of strategies for assessing students' strengths and weaknesses, designing targeted interventions, and incorporating students' cultural and linguistic backgrounds into the learning process.One of the recurring themes that has emerged from my exploration of these professional books is the importance of ongoing professional development and reflective practice. Many of the authors emphasize the need for teachers to continuously seek out new knowledge, experiment with different instructional approaches, and critically examine the effectiveness of their teaching. This has inspired me to adopt a growth mindset and to view my own learning as a lifelong journey, rather than a destination.Moreover, these resources have underscored the crucial role that collaboration plays in the field of English language teaching. The authors consistently highlight the value of working closely with colleagues, sharing best practices, and engaging in professional learning communities. This has reinforced my belief in the power of collective wisdom and the importance of fostering a collegial, supportive environment within the teaching profession.As I continue to deepen my understanding of English language instruction, I am also struck by the dynamic and evolving nature ofthe field. Many of the books I have read address the impact of technological advancements, changing educational policies, and shifting societal trends on the teaching and learning of English. This has encouraged me to stay attuned to these emerging developments and to be adaptable in my approach, constantly seeking out new ways to engage and empower my future students.In conclusion, the professional literature I have explored has been instrumental in shaping my vision for my future career as a primary school English teacher. The insights and strategies I have gained have not only enriched my knowledge but have also inspired me to become a more reflective, collaborative, and responsive educator. As I embark on this journey, I am filled with a renewed sense of purpose and a deep commitment to making a positive difference in the lives of my students.。
英文名著读书报告(范文)

英文名著读书报告英文名著读书报告篇一:简爱世界名著全英文读后感全英文读书笔记英语专业大学本科F eelings fReading JA NE EYRE Us eful expre ssin 1) [U seful expr essin] (p.47) If all the rld h ated yu, a nd believe d yu icked, hile yur n cnscien ce apprved yu, and a bslved yufrm guilt, yu uld nt be ithutfriends. 2) [Usefulexpressin] (p.231)P rejudices,it is ell knn, as m st difficu lt t eradi cate frm t he heart h se sil has never bee n lsened r fertilize d by educa tin: theygr there,firm as ee ds amng st nes. 3) [U seful expr essin] (p.40) Life a ppears t m e t shrt t be spentin nursing animsityr register ing rngs.The type f materials in this b k is ritte n in Engli sh, and th e tpic are a is liter ature. I s pent readi ng the bkfr 2 mnths. In the r ld histryf literatu re, sme ma ny classic al famus r ks are gin g t be imm rtal, butJane Eyredeeply ent ers peples sul deep ly, it byirresistib le intrins ic deep ha s attracte d the tens f thusand s f reader s deeply,has affect ed peple s inner rld, is in th e rld hist ry f liter ature theeternal cl assics.hen Jane Eyre as a baby, her pare nts died.S she hadt live ith her uncle and his f amily. Unf rtunately, her uncle als ent a ay several years lat er. Then J ane as sen t t the Lg d, hich as a charity schl andbecame a t eacher the re hen she grn up. L ater she l eft it t b ee a gvern ess f Adel a Varens,the ard fMr. EdardRchester,a Thrnfiel d Manr. La ter, Janeand Mr. Rc hester fel l in lve i th each th er, and th ey decided t get mar ried. ButMr. Rchest er as reve aled thathe had a i fe duringthe edding ceremny.S Jane lef t Thrnfiel d and Mr.Rchester.therise Mr. Rchester’s ife ascrazy. neday, she m ade a fire. Mr. Rche ster tried his bestt save his ife, butfailed and hiseyesere burntand he bec ame blind.The terri ble nes ca me t Jane, she cameback t Mr.Rchesterhurriedly. Jane tldMr. Rchest er that sh e still lv ed him a l t, and tri ed her bes t t cheerhim up. Fi nally, the y gt marri ed, and ha d a lvelybaby. This bk enthra lled me it h the char m f langua ge, and br ught hme t me the gr eat ealthf Englishrds and ph rases. But this bk i s t diffic ult t me,and I hadt lk up anumber f r ds. If the re are exp lains f sm e difficul t rds, I t hink it il l be muchbetter. Th e figure f Jane Eyre had the g reatest im pact n me.After I c lse the cv ers f thebk, afterhaving a l ng jurneyf the spir it, Jane E yre, a mar velus figu re, has le ft us s mu ch t recal l and t th ink: I rem ember hergdness, he r pursuitf justice, her self-respect an d the clea r situatin n equalit y, her str iving fr l ife, her t ughness an d her cnfi dence… The riting fthe bk iseffective, beautiful and evenfascinatin g. The aut hr is skil lful inte lling stri es. I caneasily fin d ut the c haractersf these dr amatis per snages. Be hind the n derful str y, the aut hr express ed herstrn g dissatis factins tthe sciety then. The authr thi nks that p eple shuld be gd edu cated, and everyne i s equal, n matter ma le r femal e. The nefacts that I hadn’tlearn befr e is gdnes s. I remem ber her gd ness: fr s mene h lst arms andblinded in eyes, frsmene h de spised her fr her rd inariness, and evenfr smene h had hurther deeply in the pa st. T me,I think th at I can h ardly frgi ve thse pe ple h have hurt me d eeply. May be it isgd enugh t hat nt t d sme reven ge. As a r esult f re ading this bk, her s try makesme thinkin g abut lif e and I le arn much f rm her exp erience, a t least, t hat is a f resh ne re cgnitin fthe real.I can feel that h be auty reall y is, as e are all f leshly men, s e can’t distingu ish hether a man isf nbleness r humblen ess, but f leshly men, s e can’t distingu ish hether a man isf nbleness r humblen ess, but a s there ar e greatdi fferencesin ur suls, and frmthat, e ca n kn thathether a m an is nble r rdinary, and even bscure, t hat is, he ther he is beautiful r nt. Inessence, B rnte s nve l became a direct as sault n Vi ctrian mra lity. Cntr versy base d in its r ealistic e xpsure f t hughts nce cnsidered imprper f r a lady f the 19thcentury. E mtins anyrespectabl e girl uld repress.men at thi s time ere nt t feel passin, n r ere they cnsidered sexual be ings. T cn ceive thethught f m en express ing rage a nd blatant ly retalia ting again st authrit y as defia nce agains t the trad itinal rle f men. Ja ne Eyre se nt cntrver sy thrughthe litera ry munity.Fr nt nly as it rit ten by a m an but mar ked the fi rst use frealisticcharacters. Jane s p lexity lie d in her b eing neith er hly gdr evil. Sh e as pr an d plain in a time he n sciety c nsidered a n ugly man a blt n t he face fcreatin. I t challeng ed Victria n class st ructure in a strictl y hierarch al sciety.A relatin ship betee n a lly gv erness and a ealthynbleman as simply un heard f. B rnte dre c riticism f r her atta ck n the a ristcracyh she deem ed as hypc ritical sh y but ...nt genuine. She assa ulted indi vidual s a lready est ablished m rals by pr esenting a plausible case fr b igamy.篇二:大学名著读书报告《生活在别处》的情怀“生活在别处”,记得很在以前就留意过这句话,只是当时我还不理解生活在别处的真正含义,只是知道它是米兰昆德拉的经典。
英语专业读书报告

英语专业读书报告Reading has always been an integral part of my academic journey as an English major. Throughout my studies, I have had the opportunity to delve into a vast array of literary works, each offering a unique perspective and insight into the complexities of language, culture, and the human experience. One book that has particularly resonated with me and left a lasting impression is "The Kite Runner" by Khaled Hosseini.Hosseini's novel, set against the backdrop of a turbulent Afghanistan, follows the life of Amir, a young boy from a privileged Pashtun family. The story begins with Amir's childhood, where he grapples with the guilt of his inaction during a traumatic event that shaped his relationship with his best friend, Hassan. As the narrative unfolds, Amir is forced to confront his past and make difficult choices that will ultimately determine the course of his life.What struck me most about this book was the author's masterful storytelling and his ability to weave together themes of redemption, loyalty, and the enduring power of friendship. Hosseini's vividdescriptions and the depth of his characters' emotional journeys drew me in from the very first page. The novel's exploration of the complex social and political landscape of Afghanistan also provided me with a deeper understanding of the country's turbulent history and the challenges faced by its people.One of the most compelling aspects of "The Kite Runner" is the way it delves into the concept of guilt and its impact on an individual's life. Amir's struggle to come to terms with his actions, or rather, inaction, during the pivotal moment in his childhood serves as a driving force throughout the narrative. The author masterfully navigates the complexities of guilt, showing how it can shape one's sense of self and the relationships they hold dear.Another aspect of the novel that I found particularly striking was the way Hosseini portrayed the power of friendship and the way it can transcend social and cultural divides. The bond between Amir and Hassan, despite their differing social statuses, is a testament to the enduring nature of human connection. The author's depiction of their relationship, with all its nuances and complexities, was both poignant and heartbreaking.As an English major, I was also deeply impressed by Hosseini's command of language and his ability to craft a narrative that is both emotionally resonant and intellectually engaging. The author's use ofimagery and symbolism, particularly in his depiction of the kite-flying scenes, added a layer of depth and metaphorical significance to the story. The way he wove together the personal and the political, the individual and the societal, was a testament to his skill as a writer.In addition to the literary merits of the novel, "The Kite Runner" also provided me with a deeper understanding of the cultural and historical context of Afghanistan. Hosseini's vivid descriptions of the country's landscape, customs, and social dynamics offered me a window into a world that was previously unfamiliar to me. This exposure to a different cultural perspective has been invaluable in broadening my understanding of the diversity of human experiences.As I reflect on my experience of reading "The Kite Runner," I am struck by the way it has influenced my own personal growth and development as an English major. The novel's exploration of themes such as guilt, redemption, and the power of human connection have resonated deeply with me, prompting me to engage in deeper introspection and to consider the ways in which literature can shape our understanding of the world around us.Moreover, the novel's success as a work of contemporary fiction has also inspired me to consider the role of literature in shaping our cultural and social discourse. Hosseini's ability to tackle complex and sensitive issues with nuance and empathy has demonstrated to methe potential for literature to serve as a powerful tool for social change and cultural understanding.In conclusion, "The Kite Runner" by Khaled Hosseini has left an indelible mark on me as an English major. The novel's compelling narrative, rich character development, and exploration of universal themes have not only expanded my literary horizons but have also challenged me to think more deeply about the role of literature in our lives. As I continue my academic journey, I am grateful for the opportunity to have engaged with this remarkable work of fiction and to have been able to draw upon its insights and lessons in my own personal and intellectual growth.。
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• 关键词:羊水细胞; Ad5载体;永生细胞;神经元分化; 神经营养因子
1. 引言
• 羊水细胞(AFCs)被广泛用于遗传突变的产前检查,但 关于这些细胞起源和鉴定的信息很少。 • 羊水细胞可分成经典的三大类:A.类上皮E型细胞; B.羊水 特定AF型细胞; C.纤维原F型细胞。 • 在确定条件下,羊膜上皮细胞已被报道可分化为能合成并 分泌神经递素的成熟神经细胞,这暗示了一些羊膜上皮细 胞可能保持多能干细胞的表现型。
3.1 转化羊水细 胞的体外鉴定 (二)
3.2 神经标记 表达的免疫细 胞化学检测
3.3 神经营养因子的 检测
4. 讨论
• 一些人认为,像HEK-293和HER等Ad5E1转化的人细胞系 是由神经细胞衍生而来的,本文的目的是研究Ad5E1转化 的人类羊水衍生细胞是否也具有神经元特性。 • 由Ad5E1基因作用羊水衍生细胞的转化产生了一类成长细 胞系,该细胞系展示了与上皮细胞一致的表型。
• 虽然这些细胞不显示任何典型神经细胞形态,但神经的前 体细胞以及如GFAP等神经胶质谱系标记的表达可以被证 实。 • PCR数据显示神经营养因子NGF和BDNF在培养的转化羊 水细胞中存在,且该转化羊水细胞是处于未刺激条件下。
• 使用腺病毒转化羊水衍生细胞作为提供生长因子的来 源比使用神经干细胞(该细胞很难获得)作为神经营 养因子供应者更有可行性。本研究显示通过腺病毒转 化及适当的培养细胞,并利用这些细胞所产生的神经 营养因子来治疗CNS疾病比初始提出的方案更快速。 • Ad5 E1转化羊水源细胞的神经特性与Ad5 E1转化 HEK-293细胞一致,它们能表达大量蛋白质及兔脑疫 苗NF-M,NF-L的mRNA;这些蛋白质在神经元中限 制性表达,这就提出了Ad5 E1转化细胞可能优先地影 响早期分化神经细胞。 • 在将来,利用转化羊水衍生细胞的神经源性去发展新 的分化模型,并使之在神经变性疾病中得到使用。
• 若干人胚视网膜细胞系能通过Ad5 E1转化产生,这暗示 了Ad5 E1可能优先转化人神经细胞系。 • 本文研究了神经元标记蛋白在E1转化羊水衍生细胞中的 表达。结果显示,Ad5 E1转化羊水源性细胞系的细胞能 表达上皮标记细胞角蛋白。此外,未受刺激的转化细胞
和在神经诱导介质中培养的细胞一样,表达各种神经元
的和神经胶质的标记蛋白。
2. 材料与方法
1)羊水细胞转化和细胞系增殖;
2)羊水细胞的细胞培养;
3)在低氧压下培养; 4)永生羊水源细胞的神经诱导;
5)海拉细胞的培养;
6)RT-PCR分析; 7)内源性分泌的神经营养因子的测量; 8)细胞的免疫细胞化学染色; 9)统计分析。
3. 结果
3.1 转化羊水 细胞的体外鉴 定(一)
专业英语读书报告
腺病毒转化后羊水衍生细胞的 神经元特性
Neuronal characteristics denoviral transformation
报告人:许洁 06生物工程
摘要
• 通过人腺病毒(Ad5)E1基因功能的作用,初期人类羊水细胞的高效 转化会在稳定细胞系中发生,该细胞系还能展示上皮样细胞的形态学 特性。 • 本研究利用免疫细胞化学方法证实了上皮细胞标记物的表达,显示神 经元以及诸如波形蛋白、A2B5、GFAP等神经胶质标记蛋白的表达 ,此外,神经营养因子也能在转化羊水细胞的细胞培养上浮物中检测 出来。 • 本研究结果与之前Ad5 E1转化的胚肾细胞系(HEK-293)实验结果 一致,且提出了一种关于上皮细胞和E1转化羊水衍生细胞神经元标 记蛋白共同表达及神经元样细胞优先转化的假说。