常用pGEX载体图谱

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pGEX-4T-1

Version 03302011 pGEX-4T-1 VectorspGEX-4T-1 载体Cat. No. CW2198保存：-20℃组分说明Cat. No. CW2198pGEX4T-1 Vectors 20 μl（100 ng/μl）产品简介pGEX-4T-1载体是一种常用的原核表达载体，具有Amp抗性，质粒大小为4.9 kb。

该载体多克隆位点区域含有多个常用的内切酶位点序列，便于不同基因的克隆；且全序列中含有tac启动子、GST标签序列，是一种高效的蛋白表达载体。

Tac 启动子在没有诱导物存在的情况下，质粒上携带的lacI q基因产物可以有效抑制tac启动子的转录。

可在BL21、Rosstea等表达菌株中高表达融合有GST标签的蛋白，且用凝血酶可将目的蛋白的GST标签切掉，便于下游蛋白纯化。

载体图谱及多克隆位点附表标记序列位点标记序列位点AmpR_promotor1307-1335 Orf2 3441-4400 Orf2 258-977 pGEX_5_primer 869-891 GST 258-977 tac_promotor 184-212 Orf2 1377-2237 lacI 3309-4400 lac_promotor 4449-4478 M13_forward20_primer4559-4543 lacZ_a 4540-4695 M13_reverse_primer4513-4531 M13_pUC_fwd_primer 4574-4552 M13_pUC_rev_primer 224-246 Ampicilin 1377-2237 pGEX_3_primer 1041-1019 Pbr322_origin 2392-3011 M13_pUC_rev_primer 4492-4514。

质粒图谱大全

〔转载〕Pllp-OmpA, pllp-STII, pMBP-P, pMBP-C,pET-GST, pET-Trx, pET-His, pET-CKS, pET-DsbApTZ19RDNApUC57DNAPMD18TPQE30pUC18pUC19pTrcHisApTrxFuspRSET-ApRSET-BpVAX1PBR322pbv220pBluescriptIIKS( )L4440pCAMBIA-1301pMAL-p2XpGD926ProteinExpression?ProkaryoticExpression?pETDsbFusionSystems39band40b ProteinExpression?ProkaryoticExpression?pETExpressionSystem33b ProteinExpression?ProkaryoticExpression?pETExpressionSystems ProteinExpression?ProkaryoticExpression?pETExpressionSystemsplusCompetentCells ProteinExpression?ProkaryoticExpression?pETGSTFusionSystems41and42 ProteinExpression?ProkaryoticExpression?pETVectorDNAProteinPurification?PurificationSystems?Strep?TactinResinsandPurificationKitsTEcoR?pGEX-1I/BAPpGEX-2TpGEX-2TKpGEX-3XpGEX-4T-1pGEX-4T-2pGEX-4T-3pGEX-5X-1pGEX-5X-2pGEX-5X-3pGEX-6P-1pGEX-6P-2pGEX-6P-3PTYB1PTYB2PTYB11PTYB12pCDNA3.1(-)pCDNA3.1( )pPICZalphaApGAPZαApBI121pEGFP-N1pEGFP-C1pPIC9K如何阅读分析质粒图谱载体主要有病毒和非病毒两大类,其中质粒DNA是一种新的非病毒转基因载体。

质粒图谱大全

（转载）一.九种表达载体Pllp-OmpA, pllp-STII, pMBP-P, pMBP-C,pET-GST, pET-Trx, pET-His, pET-CKS, pET-DsbA二.克隆载体pTZ19RDNApUC57DNAPMD18TPQE30pUC18pUC19pTrcHisApTrxFuspRSET-ApRSET-BpVAX1PBR322pbv220pBluescriptIIKS( )L4440pCAMBIA-1301pMAL-p2XpGD926三.PET系列表达载体ProteinExpression?ProkaryoticExpression?pETDsbFusionSystems39band40b ProteinExpression?ProkaryoticExpression?pETExpressionSystem33b ProteinExpression?ProkaryoticExpression?pETExpressionSystems ProteinExpression?ProkaryoticExpression?pETExpressionSystemsplusCompetentCells ProteinExpression?ProkaryoticExpression?pETGSTFusionSystems41and42 ProteinExpression?ProkaryoticExpression?pETNusAFusionSystems43.1and44 ProteinExpression?ProkaryoticExpression?pETVectorDNAProteinPurification?PurificationSystems?Strep?TactinResinsandPurificationKits四.PGEX系列表达载体TEcoR?pGEX-1I/BAPpGEX-2TpGEX-2TKpGEX-3XpGEX-4T-1pGEX-4T-2pGEX-4T-3pGEX-5X-1pGEX-5X-2pGEX-5X-3pGEX-6P-1pGEX-6P-2pGEX-6P-3五.PTYBsystemPTYB1PTYB2PTYB11PTYB12六.真核表达载体pCDNA3.1(-)pCDNA3.1( )pPICZalphaApGAPZαAPYES2.0pBI121pEGFP-N1pEGFP-C1pPIC9KpPIC3.5K如何阅读分析质粒图谱载体主要有病毒和非病毒两大类,其中质粒DNA是一种新的非病毒转基因载体。

质粒图谱大全

（转载）一. 九种表达载体Pllp-OmpA, pllp-STII, pMBP-P, pMBP-C,pET-GST, pET-Trx, pET-His, pET-CKS, pET-DsbA二. 克隆载体pTZ19RDNApUC57DNAPMD18TPQE30pUC18pUC19pTrcHisApTrxFuspRSET-ApRSET-BpVAX1PBR322pbv220pBluescriptIIKS( )L4440pCAMBIA-1301pMAL-p2XpGD926三.PET 系列表达载体ProteinExpression?ProkaryoticExpression?pETDsbFusionSystems39band40b ProteinExpression?ProkaryoticExpression?pETExpressionSystem33b ProteinExpression?ProkaryoticExpression?pETExpressionSystems ProteinExpression?ProkaryoticExpression?pETExpressionSystemsplusCompetentCells ProteinExpression?ProkaryoticExpression?pETGSTFusionSystems41and42 ProteinExpression?ProkaryoticExpression?pETNusAFusionSystems43.1and44 ProteinExpression?ProkaryoticExpression?pETVectorDNAProteinPurification?PurificationSystems?Strep?TactinResinsandPurificationKits四.PGEX 系列表达载体TEcoR?pGEX-1I/BAPpGEX-2TpGEX-2TKpGEX-3XpGEX-4T-1pGEX-4T-2pGEX-4T-3pGEX-5X-1pGEX-5X-2pGEX-5X-3pGEX-6P-1pGEX-6P-2pGEX-6P-3五.PTYBsystemPTYB1PTYB2PTYB11PTYB12六. 真核表达载体pCDNA3.1(-)pCDNA3.1( )pPICZalphaApGAPZα APYES2.0pBI121pEGFP-N1pEGFP-C1pPIC9KpPIC3.5K如何阅读分析质粒图谱载体主要有病毒和非病毒两大类, 其中质粒DNA是一种新的非病毒转基因载体。

pGEX质粒图谱

pGEX-1λT (27-4805-01)pGEX-6P-1 (27-4597-01)EcoR I Sma I Sal I Xho I Not IBamH I PreScission ™ ProteaseLeu Glu Val Leu Phe Gln Gly Pro Leu Gly Ser Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro HisCTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCG GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT↓pGEX-6P-2 (27-4598-01)BamH I PreScission ™ ProteaseLeu Glu Val Leu Phe Gln Gly Pro Leu Gly Ser Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala SerCTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCA GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG↓EcoR I Sal I Xho INot I pGEX-6P-3 (27-4599-01)BamH IPreScission ™ ProteaseLeu Glu Val Leu Phe Gln Gly Pro Leu Gly Ser Pro Asn Ser Arg Val Asp Ser Ser Gly ArgCTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCG AAT TCC CGG GTC GAC TCG AGC GGC CGC↓EcoR I Sma I Sal I Xho I Not I BamH IEcoR I Sma I Sal I Xho I Not I BamH I EcoR I Sma I Sal I Xho I Not IBamH I EcoR I Sma I Sal I Xho I Not IBamH I EcoR I Sma I Sal I Xho I Not IBamH I EcoR I Sma I Sal I Xho INot I BamH I EcoR I Sma I Sal I Not IEcoR I CTG GTT CCG CGT GGA TCC CCG GAA TTC ATC GTG ACT GAC TGA CGABamH ILeu Val Pro Arg Gly Ser Pro Glu Phe Ile Val Thr AspThrombinStop codons ↓pGEX~4900 bppBR322oriBal I BspM IPtac g lu ta thi on e S -t r a n s f e r as eA mp ra c Iq Nar I EcoR VBssH IIBstE IIMlu IApa ITth111 IAat IIPst Ip4.5AlwN IpSj10 Bam7Stop7∆pGEX-4T-2 (27-4581-01)pGEX-5X-1 (27-4584-01)pGEX-5X-2 (27-4585-01)pGEX-5X-3 (27-4586-01)pGEX-4T-1 (27-4580-01)pGEX-4T-3 (27-4583-01)pGEX-3X (27-4803-01)pGEX-2TK (27-4587-01)Leu Val Pr o Ar g Gly Ser Pr o Gly Ile Pr o Gly Ser Thr Ar g Ala Ala Ala SerCTG GTT CCG CGT GGA TCC CCA GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG TGAStop codon Ile Glu Gly Arg Gly Ile Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His Arg AspATC GAA GGT CGT GGG ATC CCC GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT CGT GAC TGAStop codons Ile Glu Gly Arg Gly Ile Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala SerATC GAA GGT CGT GGG ATC CCC GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG TGAStop codon Ile Glu Gly Arg Gly Ile Pro Arg Asn Ser Arg Val Asp Ser Ser Gly Arg Ile Val Thr AspATC GAA GGT CGT GGG ATC CCC AGG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGAStop codons Leu Val Pro Arg Gly Ser Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His Arg AspCTG GTT CCG CGT GGA TCC CCG GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT CGT GAC TGAStop codons Leu Val Pro Arg Gly Ser Pro Asn Ser Arg Val Asp Ser Ser Gly Arg Ile Val Thr AspCTG GTT CCG CGT GGA TCC CCG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGAStop codons ATC GAA GGT CGT GGG ATC CCC GGG AAT TCA TCG TGA CTG ACT GACIle Glu Gly Arg Gly Ile Pro Gly Asn Ser SerStop codons Leu Val Pro Arg Gly Ser Arg Arg Ala Ser ValKinaseCTG GTT CCG CGT GGA TCT CGT CGT GCA TCT GTT GGA TCC CCG GGA ATT CAT CGT GAC TGAStop codons Thrombin↓Thrombin↓Thrombin↓Thrombin↓Factor Xa↓Factor Xa↓Factor Xa↓Factor Xa↓EcoR IBamH I Sma I EcoR IBamH I Sma I pGEX-2T (27-4801-01)CTG GTT CCG CGT GGA TCC CCG GGA ATT CAT CGT GAC TGA CTG ACGLeu Val Pro Arg Gly Ser Pro Gly Ile His Arg AspStop codons EcoR IThrombin↓BamH I Sma I。

质粒图谱大全

pGEX-4T-1-USP原核表达载体构建

1.
Forward: EcoR I
Reverse: Xho I
2.原核载体的构建
片段的克隆：
载体0.5 2XP pfu buffer 25
Dntps （2.5mM ） 8 Primer-f 1 Primer-R 1 KOD E
1 酶切： dd water 24 10X buffer 4 DNA 10 EcoR I 1
dd water 32.5 10X buffer 4 DNA 2 ug EcoR I 1 Xho I 1 Total
40 ul
37℃，2 h 。

做两个单酶切位点，加上一个未酶切的载体做对照，一起跑胶。

回收。

连接： Vector DNA 2 Insert DNA 6 10X buffer 1.5 T4 DNA ligase
1 For 22℃，
2 h
取7.5 ul 转化涂板。

同时取空载和感受态涂板做对照。

小提鉴定。

测序。

{
⑴冰上20分钟-→热激：42℃、90秒-→冰上2
分钟 ⑵图板（相应抗性的LB 固体平板）
⑶12-16h 后收板，挑单克隆，小提摇菌。

图为：取500 ul菌液，离心加入1Xcracking buffer，13000rpm，离心15min，然后取15ul上样检测，得到如上图，其中L11，L22和L32分子量明显比其他的大，猜测为连接成功的质粒。

然后小提，酶切检测，片段大小为1700bp左右。

确认载体片段插入成功（第四道为空载）。

然后取10ul质粒送测序。

载体图谱

一、pGADT7(Amp)酵母表达载体(AD-Amp)AD forward primer: 5'-TAATACGACTCACTATAGGGC-3'AD reverse primer: 5'-AGATGGTGCACGATGCACAG-3'F：GC AT CGA TAC ATG GC T TG T GCTACTCTGAR：GGCC GGATCC TTAAGAGAGGTAGCTGGGAGAD forward primer: 5'-TAATACGACTCACTATAGGGC-3'AD reverse primer: 5'-AGATGGTGCACGATGCACAG-3'二、pGBKT7(Kan0酵母表达载体(BD-Kan)三、BIFC(Kan)验证互做蛋白表达载体（德国）四、pGR107(Kan)表达载体（PVX-10kb）吴建国MCS:ClaI/SmaI/SalI(pgR107)MCS:ClaI/AscI/NotI/SalI(pgR106) 五、BIFC(Amp)载体（美国）3075（nEYFP-329bp）3076 (cEYFP-218bp)F：GGC GAATTC ATG GCTTGTGCTACTCTGA R：GGC CCTAGG AGAGAGGTAGCTGGGAG TAA TAG TGA六、RNAI(Kan)载体-pFGC5941（王宗华）七、pGEX-4T-1(Amp)原核表达载体八：pET-29a(Kan)原核表达载体九、. pEGAD(Kan)植物表达载体*XbaⅠ cleavage blocked by overlapping dam methylation. Must grow in dam-strain to cut. Note, XhoⅠ is not unique.pTRV2(a) Genome organization of TRV. The TRV-RNA1 open reading frames (ORFs) correspond to 134 and 194 kDa replicase, movement protein (MP) and a 16-kDa cysteine-rich protein. The TRV-RNA2 ORFs corresponds to coat protein (CP), and the 29.4 and 32.8 kDa proteins. gRNA, genomic RNA; sgRNA, subgenomic RNA. Asterisks (*) indicate the readthrough of 134 kDa protein.(b) TRV based VIGS vectors. TRV cDNA clones were placed in betweenthe duplicated CaMV 35S promoter (2×35S) and the nopaline synthase terminator (NOSt) in a T-DNA vector. LB and RB refer to left and right borders of T-DNA. Rz, self-cleaving ribozyme. MCS, multiple cloning sites.十、十一、1103-YFP十二：pBINPLUS十三、pBin438。

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Rosetta系列的表达菌株可以提供T7 RNA聚合酶，它能表达PET系列载体上的外源基因。

pGEX系列载体上的外源基因不需要T7 RNA聚合酶，普通的大肠杆菌经IPTG诱导即可表达Tac启动子是一组由Lac和trp启动子人工构建的杂合启动子，受Lac阻遏蛋白的负调节，它的启动能力比Lac和trp都强。

其中Tac 1是由Trp启动子的－35区加上一个合成的46 bp DNA片段(包括Pribnow 盒)和Lac操纵基因构成，Tac 12是由Trp的启动子-35区和Lac 启动子的-10区，加上Lac操纵子中的操纵基因部分和SD序列融合而成蛋白标签：pGEX4T1载体基本信息出品公司: GE别名: pGEX-4T-1, pGEX4T1, pGEX 4T 1质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4969 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4580-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX4T1载体质粒图谱和多克隆位点信息原核生物DNA复制起始点，是DNA链上独特的具有起始DNA复制功能的碱基序列。

大肠杆菌的复制起pGEX4T1载体简介pGEX4T1载体序列LOCUS pGEX-4T-1 4969 bp DNA circular SYN DEFINITION pGEX-4T-1ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifierssource 1..4969/organism="pGEX-4T-1"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer"gene 258..977/label="GST (variant)"/gene="GST (variant)"CDS 258..977/label="ORF frame 3"/label="pGEX_3_primer"promoter 1307..1335/label="AmpR_promoter"gene 1377..2237/label="Ampicillin"/gene="Ampicillin"CDS 1377..2237/label="ORF frame 3"rep_origin 2392..3011/label="pBR322_origin"misc_feature 3309..4400/label="lacI"CDS 3441..4400/label="ORF frame 3"promoter 4449..4478/label="lac_promoter"misc_feature 4492..4514/label="M13_pUC_rev_primer" promoter 4513..4531/label="M13_reverse_primer" CDS 4523..81/label="ORF frame 2"misc_feature 4540..4695/label="lacZ_a"/label="M13_forward20_primer"misc_feature complement(4552..4574)/label="M13_pUC_fwd_primer"pGEX4T2载体基本信息出品公司: GE别名: pGEX-4T-2, pGEX4T2, pGEX 4T 2 质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4970 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4581-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX4T2载体质粒图谱和多克隆位点信息pGEX4T2载体简介pGEX4T2载体序列LOCUS pGEX-4T-2 4970 bp DNA circular SYN DEFINITION pGEX-4T-2ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifiers/organism="pGEX-4T-2"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer" gene 258..978/label="GST (variant)"/gene="GST (variant)"CDS 258..974/label="ORF frame 3"misc_feature complement(1020..1042)/label="pGEX_3_primer"promoter 1308..1336/label="AmpR_promoter"gene 1378..2238/label="Ampicillin"/gene="Ampicillin"CDS 1378..2238/label="ORF frame 1"rep_origin 2393..3012/label="pBR322_origin"misc_feature 3310..4401/label="lacI"/label="ORF frame 1"promoter 4450..4479/label="lac_promoter"misc_feature 4493..4515/label="M13_pUC_rev_primer"promoter 4514..4532/label="M13_reverse_primer"CDS 4524..81/label="ORF frame 3"misc_feature 4541..4696/label="lacZ_a"promoter complement(4544..4560)/label="M13_forward20_primer" misc_feature complement(4553..4575)/label="M13_pUC_fwd_primer" pGEX4T3载体质粒图谱和多克隆位点信息pGEX4T3载体简介pGEX4T3载体序列LOCUS pGEX-4T-3 4968 bp DNA circular SYN DEFINITION pGEX-4T-3ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifierssource 1..4968/organism="pGEX-4T-3"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer" gene 258..976/label="GST (variant)"/gene="GST (variant)"CDS 258..980/label="ORF frame 3"misc_feature complement(1018..1040)/label="pGEX_3_primer"promoter 1306..1334/label="AmpR_promoter"gene 1376..2236/label="Ampicillin"/gene="Ampicillin"CDS 1376..2236/label="ORF frame 2"rep_origin 2391..3010/label="pBR322_origin"misc_feature 3308..4399/label="lacI"CDS 3440..4399/label="ORF frame 2"promoter 4448..4477/label="lac_promoter"misc_feature 4491..4513/label="M13_pUC_rev_primer"promoter 4512..4530/label="M13_reverse_primer"CDS 4522..81/label="ORF frame 1"misc_feature 4539..4694/label="lacZ_a"promoter complement(4542..4558)/label="M13_forward20_primer"misc_feature complement(4551..4573)/label="M13_pUC_fwd_primer"pGEX6P1载体基本信息出品公司: GE别名: pGEX-6P-1, pGEX6P1, pGEX 6P 1 质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4984 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4597-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX6P1载体质粒图谱和多克隆位点信息pGEX6P1载体简介pGEX6P1载体序列LOCUS pGEX-6P-1 4984 bp DNA circular SYN DEFINITION pGEX-6P-1ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifierssource 1..4984/organism="pGEX-6P-1"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer"gene 258..992/label="GST"/gene="GST"CDS 258..992/label="ORF frame 3"misc_feature 918..938/label="precision"misc_feature complement(1034..1056)/label="pGEX_3_primer"promoter 1322..1350/label="AmpR_promoter"gene 1392..2252/label="Ampicillin"/gene="Ampicillin"CDS 1392..2252/label="ORF frame 3"rep_origin 2407..3026/label="pBR322_origin"misc_feature 3324..4415/label="lacI"CDS 3456..4415/label="ORF frame 3"promoter 4464..4493/label="lac_promoter"misc_feature 4507..4529/label="M13_pUC_rev_primer"promoter 4528..4546/label="M13_reverse_primer"CDS 4538..81/label="ORF frame 2"misc_feature 4555..4710/label="lacZ_a"promoter complement(4558..4574)/label="M13_forward20_primer" misc_feature complement(4567..4589)/label="M13_pUC_fwd_primer"pET28a载体基本信息出品公司: EMD Biosciences (Novagen)别名: pET28a, pet 28a, pET-28a(+) 质粒类型: 大肠杆菌蛋白表达表达水平: 高克隆方法: 多克隆位点，限制性内切酶载体大小: 5369 bp5' 测序引物及序列: T7: 5'-TAATACGACTCACTATAGGG-3' 3' 测序引物序列: T7t: 5'-GCTAGTTATTGCTCAGCGG-3' 载体标签: N-His, N-Thrombin, N-T7, C-His载体抗性: 卡那备注: N端含有Thrombin蛋白酶切位点; pET28a, b, c 的差异仅仅存在于多克隆位点处。