分泌型磷脂酶A2(sPLA2)ELISA试剂盒

分泌型磷脂酶A2(sPLA2)ELISA试剂盒分泌型磷脂酶A2(sPLA2)ELISA试剂盒产品规格：96T/48T。

供应商：上海乔羽生物有限公司上海乔羽有限公司，有elisa试剂盒，抗体，培养基分泌型磷脂酶A2(sPLA2)ELISA试剂盒Kit operation procedure:Temperature and education: 30 minutes after the closure of the plate with a sealing plate, 37.With liquid: 30 (48T of 20 times) times the concentration of washing liquid with distilled water 30 (20 times the 48T) diluted after the backup.Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.Add enzyme: each hole is added with the enzyme labeled reagent 50 mu L, except the blank hole.Sample collection and storageSerum - using a serum separating tube to make the sample at room temperature or clot for two hours overnightCentrifugation of freshly prepared serum was measured at approximately 1000 x G. 4oC for 20 minImmediately or store the samples at -20 degrees C or diluted with 80oC. Avoid repeated freezing and thawing cycles.Plasma plasma collection with EDTA or heparin as an anticoagulant. 15 minutes, centrifugal samplesX G in 2 to 8 degrees Celsius for 30 minutes. Removal of plasma and immediate detection or storage of samplesLiquid type specimen:Including serum, plasma, urine, pleural effusion and ascites, cerebrospinal fluid, cell culture supernatant, etc..Serum:Room temperature blood natural coagulation 10-20 minutes after the centrifuge about 20 minutes (2000-3000 turn / min). Carefully collect the supernatant. In the process of preservation, if there is precipitation, should be re - centrifugal.Plasma):According to the requirements of the specimens of choice of EDTA, sodium citrate or heparin as an anticoagulant, 10-20 minutes after mixing, centrifuged for 20 minutes or so (2000-3000 r.p.m.). Carefully collect the supernatant. In the process of preservation, if there is precipitation, should be re - centrifugal.Urine:Collecting with sterile tubes. Centrifugal 20 minutes or so (2000-3000 turn / min). Carefully collect the supernatant. In the process of preservation, if there is precipitation, should be re - centrifugal. Pleural effusion, cerebrospinal fluid with reference to this practice.Cell culture supernatant:To collect the ingredients for the detection of secretory components. Centrifugal 20 minutes or so (2000-3000 turn / min). Carefully collect the supernatant. Detection of cell components, with PBS (PH7.2-7.4) diluted cell suspension, the cell concentration reached 1 million /ml or so. Through repeated freezing and thawing, in order to make the cell damage and release of intracellular components. Centrifugal 20 minutes or so (2000-3000 turn / min). Carefully collect the supernatant. In the process of preservation, if there is precipitation, should be re - centrifugal.Tissue specimen:After cutting the specimen, weigh the weight. Add a certain amount of PH7.4, PBS. Rapid freezing with liquid nitrogen. The specimens were melted and still maintained at 2-8. Add a certain amount of PBS (PH7.4), with a hand or a homogenate of the specimen homogenate full. Centrifugal 20 minutes or so (2000-3000 turn / min). Carefully collect the supernatant. Be detected after a repackaging, remaining frozen spare.分泌型磷脂酶A2(sPLA2)ELISA试剂盒CalculationTo standard concentration as the abscissa, OD value as the ordinate, draw the standard curve on coordinate paper, according to the OD value of samples from the standard curve found corresponding concentration; multiplied by the dilution multiple; or with the standard concentration and the OD value calculated standard curve of the linear regression equation, the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor is the actual concentration of the sample.分泌型磷脂酶A2(sPLA2)ELISA试剂盒ELISA技术原理：以免疫学反应为基础，将抗原、抗体的特异性反应与酶对底物的高效催化作用相结合起来.1. 抗原或抗体的固相化及抗原或抗体的酶标记。

2. 结合在固相载体表面的抗原或抗体仍保持其免疫学活性。

3. 酶标记的抗原或抗体既保留其免疫学活性，又保留酶的活性。

4. 受检标本与固相载体表面的抗原或抗体起反应。

再加入酶标记的抗原或抗体，也通过反应而结合在固相载体上。

5. 此时固相上的酶量与标本中受检物质的量呈一定的比例。

6. 加入酶反应的底物后，底物被酶催化成为有色产物，产物的量与标本中受检物质的量直接相关，故可根据呈色的深浅进行定性或定量分析。

测定方法具有很高的敏感度(pg-ng/ml 水平)，并且重复性好。

分泌型磷脂酶A2(sPLA2)ELISA试剂盒Kit composition130 times concentrated washing liquid 20ml * 1 bottles; 2 enzyme labeled reagent 6ml * 1 bottle3 enzyme labeled plate 8 hole * * 12;4 sample dilution 6ml * 1 bottle5 color reagent A liquid 6ml * 1 bottle;6 color agent B liquid 6ml * 1/ bottle7 termination solution 6ml * 1 bottle;8 standard product (48ng/ml) 0.5ml * 1 bottle9 standard 1.5ml * 1 bottle;10 Manual 1 copies11 plate film 2; 12 sealing bag 1分泌型磷脂酶A2(sPLA2)ELISA试剂盒，猪促生长激素释放激素(GHRH)ELISA试剂盒，促生长激素释放激素elisakit，猪elisa试剂盒，GHRHelisakit上海乔羽生物供应！友情提醒：公司经营的产品均为科研实验用，不可用于临床应用。