重组人骨形态发生蛋白2复合骨在腰椎融合中的效果

重组人骨形态发生蛋白2复合骨在腰椎融合中的效果
王展;张军;王登峰;邵宇雄;魏巍;朱养均;王卫
【期刊名称】《中国组织工程研究》
【年(卷),期】2015(000)025
【摘要】背景：诸多研究已证实重组人骨形态发生蛋白2在骨形成及骨折愈合中发挥十分重要的作用，但单纯予以重组人骨形态发生蛋白2植入容易出现扩散和降解，无法对新骨形成予以持续性的影响。

目的：观察重组人骨形态发生蛋白2复合骨在兔腰椎中的融合效果。

方法：取30只新西兰大白兔，构建后路腰椎横突间植骨融合模型，随机均分为3组，分别在L5-6横突间植入自体髂骨、同种异体骨及重组人骨形态发生蛋白2复合骨(重组人骨形态发生蛋白2与同种异体骨复合物)，植入后6周，进行大体观察、X射线检查及组织学观察。

结果与结论：重组人骨形态发生蛋白2复合骨组融合率、新生骨组织在总面积中所占百分比高于自体髂骨组、同种异体骨组(P <0.05)；重组人骨形态发生蛋白2复合骨组、自体髂骨组拉伸强度大于同种异体骨组(P <0.05)，前两组拉伸强度无差异。

X射线显示3组植骨区均可见骨痂形成；组织学显示，自体髂骨组移植物已消失，形成大量软骨，有少量骨小梁，并有一定编织骨形成；同种异体骨组有较多的纤维组织包裹材料，有骨岛形成，有数量较少的骨小梁及软骨形成；重组人骨形态发生蛋白2复合骨组存在大量骨小梁和少量软骨，形成编织骨且有皮质骨形成。

表明重组人骨形态发生蛋白2复合骨在兔腰椎中可以获得良好的融合效
果。

%BACKGROUND:Many studies have confirmed that recombinant human bone morphogenetic protein 2 plays a very important role in bone formation and fracture healing, but recombinant human bone
morphogenetic protein 2 alone implanted is prone to diffusion and degradation, which is unable to play a persistent role in new bone formation. OBJECTIVE:To explore the effect of recombinant human bone morphogenetic protein 2 composite bone in the rabbit lumbar fusion. METHODS:Thirty New Zealand white rabbits were selected to make posterior lumbar intertransverse fusion models, and then were randomly divided into three groups, in which, L5-6 intertransverse implantation of autologous iliac bone, al ogeneic bone and recombinant human bone morphogenetic protein 2 composite bone (recombinant human bone morphogenetic protein 2 and al ogeneic bone complex) was done respectively. At 6 weeks after implantation, gross observation, X-ray examination and histological observation were performed. RESULTS AND CONCLUSION:Fusion rate and percentage of new bone area were higher in the composite bone group than the autologous iliac bone and al ogeneic bone groups (P<0.05);the tensile strength was lower in the al ogeneic bone group than the other two groups (P<0.05), but there was no difference between these two groups&nbsp;except the al ogeneic bone group. X-ray films showed cal us formation in the implanted region of the three groups. In the autologous iliac bone group, a large amount of cartilage tissues formed along with a smal amount of bone trabeculae and a certain amount of woven bones. In the al ogeneic bone group, the implant was covered with a large amount of fibrous tissues, bone island was seen and there was also a smal amount of bone trabeculae and cartilage tissues. In the composite bone group, a great amount of bone
trabeculae and cartilage tissues were visible to form woven bone and cortical bone. These findings indicate that the recombinant human bone morphogenetic protein 2 composite bone can obtain good effect in the rabbit lumbar fusion.
【总页数】5页(P3957-3961)
【作者】王展;张军;王登峰;邵宇雄;魏巍;朱养均;王卫
【作者单位】西安交通大学附属红会医院创伤骨科，陕西省西安市710054;西安
交通大学附属红会医院创伤骨科，陕西省西安市710054;西安交通大学附属红会
医院创伤骨科，陕西省西安市710054;西安交通大学附属红会医院创伤骨科，陕
西省西安市710054;西安交通大学附属红会医院创伤骨科，陕西省西安市710054;西安交通大学附属红会医院创伤骨科，陕西省西安市710054;西安交通大学第二
附属医院，陕西省西安市 710004
【正文语种】中文
【中图分类】R318
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