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复配抗氧剂对橄榄油的抗氧化性表现及其研究

复配抗氧剂对橄榄油的抗氧化性表现及其研究

合成材料老化与应用2023年第52卷第6期5复配抗氧剂对橄榄油的抗氧化性表现及其研究*时 宪,杨家庆,王秀娟὇青岛科技大学Ὃ高分子科学与工程学院Ὃ橡塑材料与工程教育部重点实验室/山东省橡塑材料与工程重点实验室Ὃ山东青岛 266042Ὀ摘要:目前,抗氧剂协同作用是抗氧剂研究领域中的热点,主要集中在天然抗氧剂之间协同作用的研究上。

该文主要研究迷迭香提取物(RE)、茶多酚提取物(TP)和特丁基对苯二酚(TBHQ)三种抗氧剂以及两两复配情况下对橄榄油的抗氧化性表现。

通过红外全反射光谱检测、DPPH 自由基清除能力和DSC 氧化诱导期的测定,探究复配抗氧剂对橄榄油储藏过程中抗氧化性的影响。

研究表明,样品在80℃烘箱中分别氧化2、4、6天时,复配抗氧剂RE 与TP 对橄榄油抗氧化性综合表现效果最佳,其次为复配抗氧剂TP 与TBHQ 。

通过研究复配抗氧剂对橄榄油抗氧化性表现,探究抗氧剂之间的协同作用,为橄榄油实际生产和生活应用中开发高效复配抗氧剂提供帮助。

关键词:橄榄油;复配抗氧剂;抗氧化性中图分类号:TS 202.3Study on Antioxidative Properties of Complex Antioxidants on Olive OilSHI Xian, YANG Jia-qing, WANG Xiu-juan( Qingdao University of Science & Technology, College of Polymer Science and Engineering, Key Laboratory of Rubber-Plastics,Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, Qingdao 266042, Shandong, China )Abstract: At present, the synergistic eff ect of antioxidants is a hot spot in the fi eld of antioxidant research, which mainly focuses on the synergistic eff ect between natural antioxidants. In this study, the antioxidant activity of rosemary extract (RE), tea polyphenol extract (TP) and tert-butylhydroquinone (TBHQ) and the antioxidant activity of olive oil in the case of pairwise combination were studied. The eff ects of complex antioxidants on the antioxidant activity of olive oil during storage were investigated by infrared total refl ection spectroscopy, DPPH free radical scavenging ability and oxidation induction period. The results showed that when olive oil was oxidized at 80℃ for 6 days, the combined antioxidant RE and TP group had the best comprehensive antioxidant eff ect on olive oil, followed by TP and TBHQ group. By exploring the antioxidant performance of complex antioxidants on olive oil, and studying the synergistic eff ect between complex antioxidants, it provides assistance for the development of effi cient complex antioxidants in the actual production and daily application of olive oil.Key words: olive oil; compound antioxidants; oxidation resistance*基金项目:国家自然科学基金(项目号52173057);山东省自然科学基金联合基金(项目号ZR2020LFG001)和橡塑材料与工程教育部重点实验室/山东省橡塑材料与工程重点实 验室青岛科技大学开放课题(项目号KF2020001)。

Unit 1 Abstracts

Unit 1 Abstracts

The pediatric burden of rotavirus disease in Europe.( from Food Science and Technology Abstracts (FETA) V ol.39 (2007) No. 1 P63)(Epidemiology and Infection 134(5) 908-916 (2006)Rotaviruses are a major cause of hospitalizations for acute gastroenteritis in developed countries. In this article, the burden of rotavirus disease in < 5-year-old children in Europe was investigated based on currently available data. Results estimated that the annual number of hospitalization for community-acquired rotavirus disease in the 23 million under 5s living in the EU-25 regions was between 72,000 and 77,000 , with a median cost of €1417 per case. Annual hospitalizations incidence rates ranged from 0.3 to 11.9/1000 children<5 year old (median 3/1000). The median proportion of hospital-acquired rotavirus disease among all cases of hospitalization for rotavirus disease was estimated to be 21%. Countries in the EU-25 require information regarding the prevalence of rotavirus disease to support introduction of rotavirus vaccines. Data on cases treated at home, medical visits and emergency wards as well as rotavirus-associated deaths were limited. It is concluded that to fully evaluate the impact and effectiveness of rotavirus vaccination program in Europe, additional epidemiological studies are required.Passage 2. Acrylamide: Review of toxicity data and dose-response analyses for cancer and non-cancer effects( from Food Science and Technology Abstracts (FETA) V ol.39 (2007) No. 1 P84) (Critical Review s in Toxicology 36 (6-7)481-608 (2006)Acrylamide (ACR) is used in the manufacture of polyacrylamides and has recently been shown to form when foods, typically containing certain nutrients, are cooked at normal cooking temperature.(e.g., frying, grilling or baking). The toxicity of ACR has been extensively investigated, and major findings have indicated that ACR is neurotoxic in animals and humans, is a reproductive toxicant in animal models, and is a rodent carcinogens. In this article, ACR toxicity data are reviewed, new relevant data are identified, and data to be used in dose-response modeling are selected. Aspects considered include: toxicokinetics (absorption, distribution, metabolism, excretion, biomarkers of exposure); hazard identification (studies in humans, neurotoxicity studies in animals, reproductive toxicity, genotoxicity, carcinogenicity bioassays potential modes of action for carcinogenic effects); and dose-response assessment (noncancer dose-response assessment, cancer dose-response assessment).Passage 3 Safety and keeping quality of pasteurized milk under refrigeration( from Food Science and Technology Abstracts (FETA) V ol.39 (2007) No. 9 P253) ( J ournal of Food Science and Technology 44 (4) 363-366 (2007)Assessment was made of the keeping quality of pasteurized milk under refrigeration (4±1℃) byevaluating its microbial, sensory and physicochemical properties on days 0,2,4,6,8,10 and 12 of storage. The initial growth rate of microorganisms was slow. Total viable counts and coliform counts of pasteurized milk increased significantly (P≦0.01) from the 4th day onwards, whereas significant increases in faecal streptococcal counts occurred from the 6th day onwards. Increases in Escherichia coli counts, psychrotrophic counts and fungal counts between day zero and day 12 were 0.2,3.7 and 2.5 log 10 cfu/ ml respectively. E.coli was isolated from 1 batch of milk on all days of storage; the isolates belonged to serotype O148 and were positive in the Congo red binding test. Staphylococcus aureus was isolated from 1 sample (5%) on the 6th day and from 2 sample (10%) on the 8th day of storage. None of samples revealed the presence of L .monocytogenes. Sensory analysis revealed that scores for color, appearance, flavor, aroma and body decreased during storage, but milk was graded as fair on the 8th day. Development of salty or stale flavor, off-odor and presence of clotted particles indicated spoilage. There was a reduction in mean pH value throughout the storage period. The samples were positive for clot formation in the boiling test on day 12 of storage but became unacceptable before this point was reached.Passage 4. Public health significance of antimicrobial-resistant Gram-negative bacteria in raw bulk tank milk(from Food Science and Technology Abstracts (FETA) V ol.39 (2007) No. 1 P250)(Foodborne Pathogens and Disease 3(3) 222-223(2006)The dairy farm environment and animals in the farm serve as important reservoirs of pathogenic and commensal bacteria that could potentially gain access to milk in the bulk tank via several pathways. Pathogenic Gram-negative bacteria can gain access to bulk tank milk from infected mammary glands, contaminated udder and milking machines, and /or from the dairy farm environment. Raw milk consumption can result in exposure to antimicrobial-resistant commensal Gram negative bacteria. This paper examines the prevalence and role of commensal Gram negative enteric bacteria in bulk tank milk and their public health significance. Aspects considered include: antimicrobial selection pressure; antimicrobial-resistant Gram-negative pathogens in bulk tank milk; antimicrobial-resistant Gram-negative commensal bacteria in bulk tank milk; and transfer of antimicrobial resistance.Passage 5. Nonenzymatic degradation of citrus pectin and pectate during prolonged heating: effects of pH, temperature, and degree of methyl esterification.(from Food Science and Technology Abstracts (FETA) V ol.39 (2007) No. 10 P16)( Journal of Agriculture and Food Chemisry 55(13) 5131-5136(2007)The underlying mechanisms governing nonenzymic pectin and pectate degradation during thermal treatment have not yet been fully eluc idated. This study determined the extent of nonenzymic degradation due toβ-elimination, acid hydrolysis and demethylation during prolonged heating of citrus pectins and its influence on physicochemical properties. The aim of the study is to improvethe quality of food products containing pectins by understanding the mechanics of pectin solubilization and degradation and how they may be minimized. Solutions of citrus pectins, buffered from pH 4.0 to 8.5, were heated at 75,85,95 and 110℃for 0-300 min. Evolution of methanol and formation of reducing groups and unsaturated uronides were monitored during heating. Molecular weight and viscosity changes were determined through size exclusion chromatorgraphy and capillary viscometry, respectively. Results showed that at pH 4.5, the activation energies of acid hydrolysis, β-elimination and demethylation were 95,136 and 98 kJ/mol, respectively. This means that at this pH, acid hydrolysis occurs more rapidly thanβ-elimination. Furthermore, the rate of acid hydrolysis is diminished by higher levels of methyl esterification. Also, citrus pectin (93% esterified) degrades primarily viaβ- elimination even under acidic conditions. Acid hydrolysis andβ- elimination caused significant reduction in relative viscosity and molecular weight.天然抗氧化剂比人工合成抗氧化剂更好更安全吗?(食品科学与技术摘要V ol.39 (2007) No. 10 P24)(欧洲油脂科学与技术杂志109 (6) 629-642 (2007)在前人所写的文章中关于讨论食物中的天然抗氧化剂已有很多。

英文 杏仁及副产品的价值

英文 杏仁及副产品的价值

ReviewThe importance of almond (Prunus amygdalus L.)and its by-productsAli Jahanban Esfahlan a,*,Rashid Jamei a ,Rana Jahanban Esfahlan ba Department of Biology,Faculty of Science,Urmia University,Urmia,IranbDepartment of Medical Biotechnology,Faculty of Medicine,Tabriz University of Medical Science,Tabriz,Irana r t i c l e i n f o Article history:Received 29June 2009Received in revised form 2September 2009Accepted 16September 2009Keywords:Almond (Prunus amygdalus L .)By-products Importancea b s t r a c tAlmond fruit consists of three or correctly four portions:kernel or meat,middle shell,outer green shell cover or almond hull and a thin leathery layer known as brown skin of meat or seedcoat.The nutritional importance of almond fruit is related to its kernel.Other parts of fruit such as shells and hulls were used as livestock feed and burned as fuel.In the past decades,different phenolic compounds were characterised and identified in almond seed extract and its skin,shell and hull as almond by-products.In addition,poly-phenols are abundant micronutrients in the human diet,and evidence for their role in the prevention of degenerative diseases such as cancer and cardiovascular diseases is emerging.The health effects of poly-phenols depend on the amount consumed and on their bioavailability.In this contribution,various pheno-lic compounds present in almond and its by-products,their antioxidant properties and potential use as natural dietary antioxidant,as well as their other beneficial compounds and applications are reviewed.Ó2009Elsevier Ltd.All rights reserved.Contents 1.Introduction .........................................................................................................3492.Almond (P.amygdalus L .)fruit characteristics ..............................................................................3503.Different parts of almond (P.amygdalus L .)fruit ............................................................................3503.1.Almond meat...................................................................................................3503.2.Almond meat brown skin.........................................................................................3513.3.Almond green shell cover (hull)....................................................................................3523.4.Almond shell ...................................................................................................3544.Potential application of almond by-products ...............................................................................3544.1.Almond shell as heavy metal adsorbent .............................................................................3544.2.Almond shell as dyes adsorbent....................................................................................3554.3.Almond shell as growing media....................................................................................3554.4.Almond shell as a rich source in preparing activated carbons............................................................3564.5.Almond shell as a source for the production of xylo-oligosaccharides (XOs)................................................3564.6.Almond by-products as dietary antioxidants..........................................................................3575.Antioxidant and antiradical activity of almond and its by-products ............................................................357References ..........................................................................................................3581.IntroductionNuts are known as a source of nutritious food with high lipid content.Replacing half of the daily fat intake with nuts has been known to lower total and LDL cholesterol levels significantly in hu-mans (Abbey,Noakes,Belling,&Nestel,1994).The observed blood cholesterol lowering effects of nuts were far better than what was predicted according to their dietary fatty acid profiles (Kendall,Jenkins,Marchie,Parker,&Connelly,2002;Kris-Etherton et al.,1999).Research also shows a connection between regular nut con-sumption and decreased incidence of coronary heart disease (Dreher,Maher,&Kearney,1996).These beneficial physiological effects suggest that bioactive compounds of nuts may possess lipid altering activities due to additive/synergistic effects and/or0308-8146/$-see front matter Ó2009Elsevier Ltd.All rights reserved.doi:10.1016/j.foodchem.2009.09.063*Corresponding author.Tel.:+989355972580;fax:+984412776707.E-mail address:a.jahanban@ (A.J.Esfahlan).Food Chemistry 120(2010)349–360Contents lists available at ScienceDirectFood Chemistryj o u r n a l h o m e p a g e :/locate/foodcheminteractions with each other.Dietary antioxidants provide protec-tion against oxidative attack by decreasing oxygen concentration, intercepting singlet oxygen,preventingfirst-chain initiation by scavenging initial radicals,binding of metal ion catalysts,decom-posing primary products of oxidation to non-radical compounds, and chain breaking to prevent continuous hydrogen removal from substrates(Shahidi,1997;Wijeratne,Abou-zaid,&Shahidi,2006).Almonds(Prunus amygdalus),which belong to the Rosaceae family that also includes apples,pears,prunes,and raspberries, are one of the most popular tree nuts on a worldwide basis and rank number one in tree nut production.They are typically used as snack foods and as ingredients in a variety of processed foods, especially in bakery and confectionery products(Sang,Chen, et al.,2002).The United States is the largest almond producer in the world and most of the US almonds are grown in California (Jahanban,Mahmoodzadeh,Hasanzadeh,Heidari,&Jamei,2009; Wijeratne et al.,2006)in an area that stretches over400miles from Bakersfield to Red Bluff(Sathe et al.,2002).Over7000individual growers cultivate more than400,000acres of almonds.Almonds are California’s largest tree crop based on dollar value,acreage, and world distribution.Five major varieties of almonds grown in California include Nonpareil,Mission,California,Neplus Ultra, and Peerless.Of thefive groups listed,most almond production (about90%)falls into three major marketing categories of Nonpareil,California,and Mission Almonds are the seeds of varieties of P.amygdalus(Sang,Lapsley,et al.,2002).Almonds,when incorporated in the diet,have been reported to reduce colon cancer risk in rats(Davis&Iwahashi,2001)and in-crease HDL cholesterol and reduce LDL cholesterol levels in hu-mans(Hyson,Schneeman,&Davis,2002).Extracts of whole almond seed,brown skin,shell,and green shell cover(hull)possess potent free radical-scavenging capacities(Amarowicz,Troszynska, &Shahidi,2005;Jahanban et al.,2009;Moure,Pazos,Medina, Dominguez,&Parajo,2007;Pinelo,Rubilar,Sineiro,&Nunez, 2004;Siriwardhana,Amarowicz,&Shahidi,2006;Siriwardhana& Shahidi,2002;Wijeratne et al.,2006).These activities may be re-lated to the presence offlavonoids and other phenolic compounds in nuts.Almond hulls have been shown to serve as a rich source of three triterpenoids(about1%of the hulls),betulinic,urosolic,and oleanolic acids(Takeoka et al.,2000),as well asflavonol glycosides and phenolic acids(Sang,Lapsley,Rosen,&Ho,2002).In addition, Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang, Lapsley,Rosen,et al.(2002)isolated catechin,protocatechuic acid, vanillic acid,p-hydroxybenzoic acid,and naringenin glucoside,as well as galactoside,glucoside,and rhamnoglucoside of3b-O-meth-ylquercetin and rhamnoglucoside of kaempferol.The production of almond hulls,which are mainly used in livestock feed,is estimated to exceed6million tons annually,thus being a potentially good source from which to extract antioxidants that are present,if any,in high quantities(Siriwardhana et al.,2006;Shahidi,Zhong, Wijeratne,&Ho,2009).2.Almond(P.amygdalus L.)fruit characteristicsThe peach-like edible almonds fruit(P.amygdalus)have three distinct parts:the inner kernel or meat,the middle shell portion, and an outer green shell cover or hull.Almond varieties vary in shell texture;therefore they are termed hard or soft shelled.The harvesting procedure starts when the almonds are partly dried on the trees(King,Miller,&Eldridge,1970).In addition the sweet almond is a stone fruit which have several unique features. It is commercially cultivated where there are long,hot,and Mediterranean like summers,such as those in Spain,Morocco, Armenia,Iran,Italy,California(USA),and Australia.It is unique, in that unlike others in its botanical family,such as peach,apricot and plum,where theflesh(mesocarp)of the fruit is eaten and the seed within its shell,or stone(endocarp)is discarded,the reverse is true for the almond early in its maturation cycle,for a period of a few weeks,the entire fruit(seed,endocarp and mesocarp)can be, and is,eaten,in several parts of the world.As the maturation cycle continues,the hull splits open.When dry,it may be readily sepa-rated from the shell.The almond pit,containing a kernel or edible seed,is the nut of commerce,the endocarp(shell),and mesocarp are separated for low value uses,such as cat litter and animal feed (Rabinowitz,1991,2002,2004).Shelled almonds may be sold as whole natural almonds or processed into various almond forms. The whole natural almonds have their shells removed but still re-tain their brown skins;blanched whole almonds have both their shells and skins ually,the removed skins are discarded (Sang,Chen,et al.,2002;Sang,Lapsley,et al.,2002;Sang,Lapsley, Rosen,et al.,2002).3.Different parts of almond(P.amygdalus L.)fruit3.1.Almond meatEdible nuts are cultivated under a variety of growing conditions and climates;they are globally popular and valued for their sen-sory,nutritional,and health attributes(Venkatachalam&Shridhar, 2006).Almond,scientifically known as Prunus dulcis,belongs to the family Rosaceae,almond tree is the number one tree nut produced on a global basis(Chen,Milbury,Lapsley,&Blumberg,2005; Wijeratne et al.,2006).It is especially spread through and well adapted to the whole Mediterranean region,from which about 28%of the world production is obtained.In fact,almond tree is an important crop,due to its fruits of high commercial value (Cordeiro&Monteiro,2001;Martins,Tenreiro,&Oliveira,2003; Moure et al.,2007).There is a great diversity of almonds which ex-hibit different productivity and yields of seed in the fruit(Martinez, Granado,Montane,Salvado,&Farriol,1995).Almond,with or without the brown skin,is consumed as the whole nut or used in various confectioneries(Wijeratne et al.,2006).It is well known that fruits and nuts contain a wide variety of phenolic acids and flavonoids that are predominantly conjugated with sugars or other polyols via O-glycosidic bonds or ester bonds(Milbury,Chen, Dolnikowski,&Blumberg,2006)and its consumption has been associated with reduced risk of chronic diseases(Pellegrini et al., 2006).The Prunus genus is reported to have interesting biological properties such as sedative,anti-inflammatory,anti-hyperlipi-demic,anti-tumoural and antioxidant activities(Donovan,Meyer, &Waterhouse,1998;Sang,Chen,et al.,2002;Wang,Nair, Strasburg,Booren,&Gray,1999).Apart from its nutritional value, almond is reported to have beneficial effects on blood cholesterol level and lipoprotein profile in humans.In addition,almonds,when used as snacks and in diets of hyperlipidemic subjects,significantly reduced coronary heart disease factors.A longterm supplementa-tion of almond showed spontaneous nutrient modification of an individual’s habitual diet that closely matched the recommen-dations to prevent cardiovascular and other chronic diseases (Wijeratne et al.,2006).Few studies reporting almond seed antioxidant potential are available.Research has only been made on bioactive compounds and their antioxidant activity in almond hulls(Takeoka et al., 2000;Sang,Chen,et al.,2002;Sang,Lapsley,et al.,2002;Takeoka &Dao,2003;Rabinowitz,2004;Jahanban et al.,2009),almond skins(Sang,Chen,et al.,2002)and almond shells(Jahanban et al.,2009;Pinelo et al.,2004).Even though it has already been demonstrated that individual almond components(Takeoka et al.,2000;Sang,Chen,et al.,2002;Sang,Lapsley,et al.,2002; Takeoka&Dao,2003;Pinelo et al.,2004;Rabinowitz,2004;350 A.J.Esfahlan et al./Food Chemistry120(2010)349–360Jahanban et al.,2009)have antioxidant potential,scientific infor-mation on antioxidant properties of whole almond is still rather scarce.Hence,the evaluation of such properties remains an inter-esting and valuable task,particularly forfinding new sources for natural antioxidants,functional foods and nutraceuticals.Barreira,Ferreira,Oliveira,and Pereira(2008)studied the anti-oxidant properties of different almond kernels(maintaining the brown skin)cultivars(cv.),either regional(Casanova,Duro Italiano,Molar,Orelha de Mula and Pegarinhos cv.)or commercial (Ferraduel,Ferranhês,Ferrastar and Guara cv.)through several chemical and biochemical assays such as DPPH radical-scavenging activity,reducing power,inhibition of b-carotene bleaching,inhibi-tion of oxidative haemolysis in erythrocytes,induced by2,20-azo-bis(2-amidinopropane)dihydrochloride(AAPH),and inhibition of thiobarbituric acid reactive substances(TBARS)formation in brain cells.Bioactive compounds such as phenols andflavonoids have been obtained and correlated to antioxidant activity.The results obtained were quite heterogeneous,revealing significant differ-ences amongst the cultivars assayed.Duro Italiano cv.revealed better antioxidant properties,presenting lower EC50values in all assays,and the highest antioxidants contents.The protective effect of this cultivar on erythrocyte biomembrane haemolysis was main-tained over a4h period.Additionally,different almond cultivars revealed significant variation in antioxidant activity,correlating with the amounts of bioactive compounds present.Using an HPLC method,phenolic compounds such as vanillic, caffeic,p-coumaric,ferulic acids(after basic hydrolysis),quercetin, kaempferol,isorhamnetin(after acidic hydrolysis),delphinidin and cyanidin(after n-butanol/HCl hydrolysis)as well as procyanidins B2and B3were determined in almond seed extract(Tables1–3, 6and8).Dominant compounds were procyanidins B2and B3 (Amarowicz et al.,2005).3.2.Almond meat brown skinTheflesh of almond seed is encased in a brown leathery coat-ing,called the seedcoat,which protects the almond from oxida-tion and microbial contamination.Many food applications of almonds in bakery and confectionary items,cereals,snack formu-lations,and marzipan,require theflesh of the almond alone without the seedcoat(Frison-Norrie&Sporns,2002).Almond (P.amygdalus)skins are agricultural by-products that are a source of phenolic compounds and are produced upon processing of al-monds in large amounts.Almond skins,resulting from hot water blanching process,are ground and used as animal feed or burned as fuel in processing plants(Harrison&Were,2007).The skins constitute about4%of the almond fruit,and are a readily avail-able source of phenolics(Chen et al.,2005).These phenolic com-pounds inhibit lipid oxidation by scavenging free radicals, chelating metals,activating antioxidant enzymes,reducing tocopherol radicals and inhibiting enzymes that cause oxidation reactions(Heim,Tagliaferro,&Bobilya,2002).Yet,recent investi-gations into the phytochemical composition of almond skins have shown that the seedcoats may contain many potentially benefi-cial compounds,opening up new possibilities for adding value to almond seedcoats.Takeoka et al.(2000)described three trit-erpenoids,betulinic acid,oleanoic acid,and ursolic acid,which have been reported to possess anti-inflammatory(Singh,Singh, &Bani,1994),anti-HIV(Kashiwada et al.,1998),and anti-cancer activities(Pisha et al.,1995).Various phenolic compounds have been identified in almond seed coats.Four differentflavonol gly-cosides,namely isorhamnetin rutinoside,isorhamnetin glucoside, kaempferol rutinoside and kaempferol glucoside have been re-ported in almond seedcoats(Frison&Sporns,2002;Frison-Norrie &Sporns,2002;Wijeratne et al.,2006)(Table4).Other investiga-tors have likewise identified phenolic compounds in almond skins including quercetin glycosylated to glucose,galactose and rham-nose,kaempferol,naringenin,catechin,protocatechuic acid,vanil-lic acid and a benzoic acid derivative(Chen et al.,2005;Monagas, Garrido,Lebron-Aguilar,Bartolome,&Gomez-Cordoves,2007) (Tables1and3–5).In addition,the isolation and identification of phenolic com-pounds in almond skins has been reported by Sang,Chen,et al. (2002).In this study,nine phenolic compounds were isolated from the ethyl acetate and n-butanol fractions of almond(P.amygdalus) skins.On the basis of NMR data,MS data,and comparison with the literature,these compounds were identified as3b-O-methylqu-ercetin3-O-a-D-glucopyranoside(1);3b-O-methylquercetin3-O-a-D-galactopyranoside(2);3b-O-methylquercetin3-O-R-L-rhamnopyranosyl-(1?6)-a-D-glucopyranoside(3);kaempferol 3-O-R-L-rhamnopyranosyl-(1?6)-a-D-glucopyranoside(4); naringenin7-O-a-D-glucopyranoside(5);catechin(6);proto-catechuic acid(7);vanillic acid(8);and p-hydroxybenzoic acid (9)(Tables1and3–5).All of these compounds have been isolated from almond skins for thefirst time.2,2-Diphenyl-1-pic-rylhydrazyl(DPPH)radical-scavenging activities of compounds 1–9showed that compounds6and7possessed very strong DPPH radical-scavenging pounds1–3,5,8,and9showed strong activity,whereas compound4exhibited a very weak activity.In another investigation,an exhaustive study of the phenolic composition of almond(Prunus dulcis(Mill.) D.A.Webb)skinsTable1Content of hydroxybenzoic acids and aldehydes in different parts of almond.Hydroxybenzoic acids and aldehydes Content ReferencesKernel Skin Shell Hullp-Hydroxybenzoic acid– 6.88–5.33a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Vanillic acid0.10b–––Amarowicz et al.(2005)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002)–14.5–7.65a––Monagas et al.(2007) Protocatechuic acid–32.0–14.5a––Monagas et al.(2007)++–+Wijeratne et al.(2006)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Protocatechuic aldehyde–20.1–11.6a––Monagas et al.(2007)+Present.–Not found.a l g/g.b mg catechin equivalents/g80%aqueous acetone extract.A.J.Esfahlan et al./Food Chemistry120(2010)349–360351carried out in order to evaluate their potential application as a functional food ingredient(Monagas et al.,2007).Using the HPLC–DAD/ESI–MS technique,a total of33compounds corre-sponding toflavanols,flavonols,dihydroflavonols andflavanones, and other non-flavonoid compounds identified.Peaks correspond-ing to another23structure-related compounds were also detected. MALDI–TOF MS was employed to characterise almond skin pro-anthocyanidins,revealing the existence of a series of A-and B-type procyanidins and propelargonidins up to heptamers,and A-and B-type prodelphinidins up to hexamers(Table3).Results showed thatflavanols andflavonol glycosides were the most abundant phenolic compounds in almond skins,representing up to38–57% and14–35%of the total quantified phenolics,respectively (Tables4–6).Due to their antioxidant properties,measured as oxy-gen-radical absorbance capacity(ORAC)at0.398–0.500mmol Trol-ox/g,almond skins can be considered as a value-added by-product for potential use as dietary antioxidant ingredients.3.3.Almond green shell cover(hull)The mesocarp of almond becomes dry,leathery,and astringent to the taste,reflecting the fact that the mature almond mesocarp has unusually a high concentration offlavonoids compared to its botanical relatives,as well as to other fruits.This is thought to be a consequence of the length of time that the mesocarp is subjected to intense heat,ultraviolet radiation,and pest infestation,as the flavonoids play protective roles against all of these stress factors. The extended maturation period of the mesocarp,flowing into remarkably stable senescence period,also allows for biosynthesis of lignans in the mesocarp,compared to the near absence of these compounds in other fruits.The mesocarp in senescence,following harvest of the nut meats,remains remarkably stable in that it re-tains its high sugars,flavonoids,and lignan content,for years,so long as the mesocarps,referred to as hulls,remain in their dry har-vested condition,having approximately8–20%moisture content,Table2Content of hydroxycinnamic acids in different parts of almond.Hydroxycinnamic acids Content ReferencesKernel Skin Shell HullFerulic acid0.02b–––Amarowicz et al.(2005)Trace 2.19±0.01c– 2.71±0.02c Siriwardhana et al.(2006) Sinapic acid Trace9.51±0.03c–9.92±0.02c Siriwardhana et al.(2006) Caffeic acid0.01b–––Amarowicz et al.(2005)Trace Trace–Trace Siriwardhana et al.(2006) p-Coumaric acid0.03b–––Amarowicz et al.(2005)Trace 3.09±0.01c– 1.34±0.01c Siriwardhana et al.(2006)–0.725c––Monagas et al.(2007) Chlorogenic acid–10.6–3.12c––Monagas et al.(2007)–––42.52±4.50a Takeoka and Dao(2003) Cryptochlorogenic acid–––7.90a Takeoka and Dao(2003) Neochlorogenic acid––– 3.04a Takeoka and Dao(2003)+Present.–Not found.a mg/100g of fresh weight.b mg catechin equivalents/g80%aqueous acetone extract.c l g/g.Table3Content of anthocyanidin and procyanidin in different parts of almond.Anthocyanidin and procyanidin Content ReferencesKernel Skin Shell HullDelphinidin0.05b–––Amarowicz et al.(2005)Cyanidin 1.76b–––Amarowicz et al.(2005)(+)-Catechin–90.1–36.4a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) (À)-Epicatechin–36.6–14.8a––Monagas et al.(2007)Procyanidin B3+B1–23.8–11.8a––Monagas et al.(2007)Procyanidin B2 1.24b–––Amarowicz et al.(2005)–16.1–5.34a––Monagas et al.(2007) Procyanidin B3 3.16b–––Amarowicz et al.(2005)Procyanidin B7–13.9–5.63a––Monagas et al.(2007)Procyanidin B5–8.57–3.46a––Monagas et al.(2007)Procyanidin C1–15.3–3.45a––Monagas et al.(2007)A-type procyanidin dimer(tr=31.3)– 6.98–3.18a––Monagas et al.(2007)A-type procyanidin dimer(tr=32.2)– 6.30–1.36a––Monagas et al.(2007)A-type procyanidin dimer(tr=35.7)–7.29–3.97a––Monagas et al.(2007)A-type procyanidin dimer(tr=48.9)– 2.04–0.70a––Monagas et al.(2007)A-type prodelphinidin dimer(tr=50.7)– 1.80–0.90a––Monagas et al.(2007)A-type procyanidin trimer(tr=30.8)– 4.28–1.58a––Monagas et al.(2007)+Present.–Not found.a l g/g.b mg catechin equivalents/g80%aqueous acetone extract.352 A.J.Esfahlan et al./Food Chemistry120(2010)349–360usually averaging about12%.In addition to these dry solubles,the hulls also contain insolublefibre,constructing of cellulose,hemi-cellulose,pectins,tannin-like complex polyphenols,and ash.As dry hulls,therefore,the almond mesocarp represent a potential source of useful foods,food additives,pharmaceuticals,and feed additives,over and above low value usage as roughage or cat litter (Rabinowitz,1991,2002,2004).In the past,almond hulls,a by-product of the almond industry,were removed from almonds after harvesting and used as supplemental livestock feed.Recently, there is interest in using almond hulls as a natural source for sweetener concentrate and dietaryfibre(Takeoka&Dao,2003).Al-mond hulls contain triterpenoids(Takeoka et al.,2000),lactones (Sang,Chen,et al.,2002),and phenolics(Sang,Lapsley,Rosen, et al.,2002).Takeoka and Dao(2003)extracted Almond hulls(Nonpareil variety)with methanol and analysed the extract,using reverse phase HPLC with diode array detection.The extract contained5-O-caffeoylquinic acid(chlorogenic acid),4-O-caffeoylquinic acid (cryptochlorogenic acid),and3-O-caffeoylquinic acid(neochloro-genic acid).The chlorogenic,cryptochlorogenic and neochlorogenicTable4Content offlavonol glycosides in different parts of almond.Flavonol glycosides Content ReferencesKernel Skin Shell HullKaempferol-3-O-rutinoside–12.8–31.8a––Monagas et al.(2007)–+––Frison and sporns(2002)++–+Wijeratne et al.(2006) Kaempferol-3-O-glucoside– 1.65a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002)–+––Frison and sporns(2002) Isorhamnetin-3-O-rutinoside–27.6–41.4a––Monagas et al.(2007)–+––Frison and sporns(2002) Isorhamnetin-3-O-glucoside–15.6–8.85a––Monagas et al.(2007)–+––Frison and sporns(2002)++–+Wijeratne et al.(2006) Quercetin-3-O-glucoside– 2.41–1.33a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Quercetin-3-O-galactoside–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002)+Present.–Not found.a l g/g.Table5Content offlavanone glycosides in different parts of almond.Flavanone glycosides Content ReferencesKernel Skin Shell HullNaringenin-7-O-glucoside–22.1–6.84a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Eriodictyol-7-O-glucoside– 1.60–0.808a––Monagas et al.(2007)+Present.–Not found.a l g/g.Table6Content offlavonol aglycones in different parts of almond.Flavonol aglycones Content ReferencesKernel Skin Shell HullKaempferol0.17b–––Amarowicz et al.(2005)– 1.71–1.96a––Monagas et al.(2007) Quercetin0.14b–––Amarowicz et al.(2005)– 1.78–1.43a––Monagas et al.(2007)++–+Wijeratne et al.(2006) Quercitrin++–+Wijeratne et al.(2006) Isorhamnetin0.15b–––Amarowicz et al.(2005)– 4.87–4.19a––Monagas et al.(2007)++–+Wijeratne et al.(2006)+Present.–Not found.a l g/g.b mg catechin equivalents/g80%aqueous acetone extract.A.J.Esfahlan et al./Food Chemistry120(2010)349–360353acid concentration of almond hulls were42.52±4.50,7.90and 3.04mg/100g of fresh weight(n=4;moisture content=11.39%) or in the ratio79.5:14.8:5.7(Table2).Extracts were also tested for their ability to inhibit the oxidation of methyl linoleate at 40°C.At an equivalent concentration(10l g/1g of methyl linole-ate)almond hull extracts had higher antioxidant activity than a-tocopherol.At higher concentrations(50l g/1g of methyl linole-ate),almond hull extracts showed increased antioxidant activity that was similar to chlorogenic acid and morin[2-(2,4-dihydroxy-phenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one]standards(at the same concentrations).These data indicate that almond hulls are a potential source of these dietary antioxidants.The sterols (3b,22E)-stigmasta-5,22-dien-3-ol(stigmasterol)and(3b)-stig-mast-5-en-3-ol(b-sitosterol)(Table8)identified by GC–MS of the silylated almond hull extract in this study.3.4.Almond shellAlmond shell is the name given to the ligneous material forming the thick endocarp or husk of the almond tree(P.amygdalus L.)fruit.When the fruit is processed to obtain the edible seeds,big lig-neous fragments are separated.These materials remain available as a waste product for which no important industrial use has been developed,so they are normally incinerated or dumped without control(Urrestarazu,Martinez,&Salas,2005).This industrial resi-due is the woody endocarp of the almond fruits.The processing by-products,shells and hulls,account for more than50%by dry weight of the almond fruits(Fadel,1999;Martinez et al.,1995). The high xylan content of almond shells makes them a suitable substrate for the production of xylose(Pou-Ilinas,Canellas, Driguez,Excoffier,&Vignon,1990),furfural(Quesada,Teffo-Bertaud,Croue,&Rubio,2002)or for fractionation into cellulose, pentosans,and lignin(Martinez et al.,1995).This latter utilisation consists of an acid-catalysed hydrolysis performed under mild con-ditions,which causes depolymerisation and solubilisation of the main components present in hemicelluloses.The liquid phase (hydrolysate)contains sugars,sugar-dehydration products,acetic acid and compounds derived from the acid-soluble lignin,which can be used for the production of oxyaromatics of interest for the health,cosmetics and food industries(Quesada et al.,2002).Al-mond shell is highly lignified(30–38%of the dry weight)(Martinez et al.,1995)and the guaiacyl to syringyl phenylpropane units ratio is similar to that of hardwoods(Quesada et al.,2002).Even if most of the lignin is acid-insoluble(Klason lignin),a part of it can be solubilised in acidic media.The antioxidant potential of depoly-merised lignin fractions produced after mild acid hydrolysis of lig-no-cellulosics has been reported(Cruz,Dominguez,&Parajo,2004, 2005;Garrote,Cruz,Dominguez,&Parajo,2003;Gonzalez,Cruz, Dominguez,&Parajo,2004).O-acetylated xylo-oligosaccharides (DXO)isolated from almond shells by autohydrolysis as well as their de-acetylated form(DeXO)were subjected to chemical, molecular,and structural analyses.They represent a mixture of neutral and acidic oligomers and low-molecular-weight polymers related to(4-O-methyl-D-glucurono)-D-xylan.DXO and DeXO showed direct mitogenic activity and enhancement of the T-mito-gen-induced proliferation of rat thymocytes,indicating the immu-nostimulatory potential of the almond shell xylo-oligosaccharides (Nabarlatz,Ebringerova,&Montane,2007).4.Potential application of almond by-products4.1.Almond shell as heavy metal adsorbentHeavy metals are nowadays amongst the most important pollu-tants in surface and ground waters.They are extremely toxic ele-ments,which can seriously affect plants and animals and have been cause a large number of afflictions(Taha,RIcordel,CIsse,& Dorange,2001).Therefore,elimination of these metals from water and wastewater is important in order to protect public health.For this reason,development of a new,flexible and environmentally friendly process for treatment of water and industrial effluents is a major challenge(Gaballah&KIlbertus,1998).The treatmentTable7Content of dihydroflavonol aglycones andflavanone aglycones phenolic compounds in different parts of almond.Dihydroflavonol aglyconesandflavanone aglyconesContent ReferencesKernel Skin Shell HullDihydroquercetin–Traces––Monagas et al.(2007) Naringenin– 2.83–4.01a––Monagas et al.(2007) Eriodictyol– 2.37–2.34a––Monagas et al.(2007) Morin++–+Wijeratne et al.(2006) +Present.–Not found.a l g/g.Table8Content of almond hulls sterols.Sterols Content ReferencesStigmasterol18.9a Takeoka and Dao(2003)b-Sitosterol16.0a Takeoka and Dao(2003)a mg/100g of almond hull.Table9Content of total phenolics in different parts of almond.Almond fruit parts Total phenolicscontentReferencesKernel16.1±0.4e Amarowicz et al.(2005)8.1±1.75a Siriwardhana and Shahidi(2002)8±1b Wijeratne et al.(2006)8±1b Siriwardhana et al.(2006)Skin87.8±1.75a Siriwardhana and Shahidi(2002)88±2b Wijeratne et al.(2006)88±2b Siriwardhana et al.(2006)413–242f Monagas et al.(2007)Shell 2.2c Moure et al.(2007)38.0±3.30d Jahanban et al.(2009)Hull71.1±1.74a Siriwardhana and Shahidi(2002)71±2b Wijeratne et al.(2006)71±2b Siriwardhana et al.(2006)78.2±3.41d Jahanban et al.(2009)a mg catechin equivalents/g ethanolic extract.b mg quercetin equivalents/g ethanolic extract.c g gallic acid equivalents/100g shells.d mg gallic acid equivalents/g methanolic extract.e mg catechin equivalents/g80%aqueous acetone extract.f l g/g.354 A.J.Esfahlan et al./Food Chemistry120(2010)349–360。

紫薯 研究

紫薯 研究

紫薯主要成分及营养保健作用研究摘要:紫薯为旋花科双子叶植物,是甘薯的一种优良新品种,呈紫红色,具有营养和保健等作用。

本文通过对紫薯中主要抗氧化化学成分及其生物活性的介绍,为更好地评价紫薯的营养价值和综合利用紫薯提供科学依据。

关键词:紫薯;花青素;花色苷;抗氧化前言紫薯(Ipomoea batatas Poir,旋花科),双子叶植物[1],又名黑红薯,是一种薯肉呈紫色,薯皮呈紫黑色的甘薯,原产地在南美洲安第斯山脉一带,后来传入亚洲和非洲,主要在日本、韩国及新西兰等国家种植[2]。

近年来我国研究人员从日本九州农业试验场引进川山紫品种并成功繁育,自此在我国四川、河南、山东等地开始大面积种植。

紫薯同其它甘薯品种一样含有丰富的淀粉、糖、蛋白质、维生素等营养物质[3],其中人体的8种必需氨基酸,紫薯含有7种(异亮氨酸、蛋氨酸、赖氨酸、苏氨酸、亮氨酸、苯丙氨酸、缬氨酸),紫薯在块根中原位合成了大量的花色苷和花青素[4],被誉为继水、蛋白质、脂肪、碳水化合物、维生素、矿物质之后的第七大必需营养素。

1 主要化学成分1.1 花青素花青素(Anthocyanidin),又称花色素,是自然界一类广泛存在于植物中的水溶性天然色素,属类黄酮化合物。

紫薯中的花青素是一种天然食用水溶性红色素,易溶于水、酸和碱,不溶于有机溶剂。

主要成分有两种,分别是芍药素和矢车菊素。

由于这两种花青素有多个酚羟基存在,导致其不太稳定,常与一个或多个半乳糖、阿拉伯糖、鼠李糖或葡萄糖等由糖苷键形成糖苷衍生物—花色苷。

1.2 酚类化合物酚类化合物是指芳香烃中苯环上的氢原子被羟基取代所生成的化合物,是芳烃的含羟基衍生物。

酚类化合物能够抑制多种突变因子引起的逆向突变。

紫薯中含有大量酚类化合物,但目前经化学成分研究的主要成分为咖啡酸及绿原酸异构体。

酚类酸对花色苷进行酰基化,能够增强和改变花色苷的颜色和稳定性[5]。

1.3花色苷类是花色素与糖以糖苷键结合而成的一类化合物,花色苷中的糖苷基和羟基还可以与一个或几个分子的阿魏酸、咖啡酸等通过酯键形成较稳定的酰基化花色苷[6]。

有关咖啡的英语阅读

有关咖啡的英语阅读

有关咖啡的英语阅读Coffee: A Brewed History, Health Impacts, and Brewing Techniques.Origins and History.The origins of coffee trace back to the ancient highlands of Ethiopia, where legend has it that a goat herder named Kaldi observed his goats becoming unusually energetic after consuming berries from a certain tree. Intrigued, he tasted the berries himself and experienced a similar invigorating effect.From Ethiopia, coffee spread to Arabia, where it gained popularity in Sufi monasteries as a stimulant for all-night prayer sessions. By the 16th century, coffee was introduced to Europe through trade with the Ottoman Empire, quickly becoming a favorite beverage among intellectuals and royalty.Varieties and Cultivation.Coffee is derived from the roasted seeds (beans) of the Coffea plant, of which there are two main species: Arabica and Robusta. Arabica, native to Ethiopia, is renowned for its delicate flavor and aroma, while Robusta, originatingin Central and West Africa, is known for its highercaffeine content and more bitter taste.Coffee plants thrive in tropical climates with richsoil and abundant rainfall. They are typically cultivatedin large plantations, where the beans are harvested by hand or machine when ripe. After harvesting, the beans are processed to remove the outer pulp and parchment, revealing the green coffee beans.Health Impacts.Coffee has been the subject of extensive scientific research, with mixed findings on its health effects. Some studies have linked moderate coffee consumption (2-4 cups per day) to potential health benefits, including:Reduced Risk of Type 2 Diabetes: Coffee contains compounds that may improve insulin sensitivity and reducethe risk of developing type 2 diabetes.Improved Cognitive Function: Caffeine, a key component of coffee, has been shown to enhance alertness, memory, and cognitive performance.Antioxidant Properties: Coffee is a rich source of antioxidants, which may help protect cells from damage and reduce the risk of chronic diseases.However, excessive coffee intake or consumption at inappropriate times can also have negative effects, such as:Anxiety and Sleep Disturbances: High levels ofcaffeine can lead to anxiety, restlessness, and disrupted sleep.Increased Blood Pressure: Coffee can temporarily raise blood pressure, especially in individuals with pre-existinghypertension.Pregnancy Risks: Excessive coffee consumption during pregnancy has been linked to an increased risk of low birth weight and premature birth.Brewing Techniques.The art of brewing coffee involves extracting the desired flavors and aromas from the roasted beans. There are numerous brewing methods, each producing a unique taste profile:Drip Coffee: Hot water is poured over ground coffee in a filter paper, allowing the brewed coffee to drip into a carafe.French Press: Ground coffee is steeped in hot waterfor a few minutes, then pressed down to separate the grounds from the coffee.Pour Over: Hot water is manually poured over groundcoffee in a cone-shaped filter, creating a smooth and balanced brew.Espresso: Finely ground coffee is tamped tightly intoa portafilter and extracted with pressurized hot water, producing a concentrated, flavorful beverage.Cold Brew: Ground coffee is steeped in cold water for an extended period (12-24 hours), resulting in a lessacidic and more mellow coffee.Cultural Significance.Coffee has become an integral part of many cultures around the world. In some countries, such as Ethiopia and Turkey, coffee ceremonies are an important social tradition. In others, coffeehouses have served as hubs forintellectual discussion and artistic expression.From its humble origins in the Ethiopian highlands toits widespread popularity today, coffee has played asignificant role in history, culture, and the daily lives of people worldwide.。

超级食物superfood

超级食物superfood

flaxseed
a great source of Omega-3 Fatty Acids contains dietary fiber as well
flaxseed
a great source of Omega-3 Fatty Acids contains dietary fiber as well The function of fiber--moderating blood glucose levels and lowering cholesterol ,etc Some research has shown that flaxseed consumption may have hearthealth benefits

fish and nuts
Walnuts are one of the best plant sources of Omega-3 Fatty Acids. They are also rich in fiber, B Vitamins, Magnesium, and antioxidants such as Vitamin E.
super foods have also been getting a lot of media attention recently from T.V.
list
Good Examples -Blueberries -Tomatoes -Flaxseed -Soy -Green Tea
-Pomegranate -Fish and nuts -Green Veggies -Oats
reduce cholesterol levels and may provide protection from some forms of cancer

Antioxidant and antimicrobial activities of tea infusions

Antioxidant and antimicrobial activities of tea infusionsM.Pilar Almajano a ,Rosa Carbo´a ,J.Angel Lo ´pez Jime ´nez b ,Michael H.Gordon c,*aChemical Engineering Department,The Technical University of Catalonia,Av.Diagonal 649,08034,Barcelona,SpainbPhysiology Department,Faculty of Biology,University of Murcia,30100Murcia,SpaincHugh Sinclair Unit of Human Nutrition,Department of Food Biosciences,University of Reading,Whiteknights,P.O.Box 226,Reading RG66AP,UKReceived 15February 2007;received in revised form 23September 2007;accepted 11October 2007AbstractTea polyphenols,especially the catechins,are potent antimicrobial and antioxidant agents,with positive effects on human health.White tea is one of the less studied teas but the flavour is more accepted than that of green tea in Europe.The concentrations of various catechins in 13different kinds of infusion were determined by capillary electrophoresis.The total polyphenol content (Folin–Ciocalteu method),the trolox equivalent antioxidant capacity (TEAC value determined with the 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)radical cation)and the inhibitory effects of infusions on the growth of some microorganisms were determined.Five different infu-sions (black,white,green and red teas and rooibos infusion)were added to a model food system,comprising a sunflower oil-in-water emulsion containing 0%or 0.2%bovine serum albumin (BSA),and the oxidative stability was studied during storage at 37°C.Oxidation of the oil was monitored by determination of the peroxide value.The highest radical-scavenging activity observed was for the green and white teas.Emulsions containing these extracts from these teas were much more stable during storage when BSA was present than when it was not present,even though BSA itself did not provide an antioxidant effect (at 0.2%concentration).Rooibos infusion did not show the same synergy with BSA.Green tea and white tea showedsimilar inhibitions of several microorganisms and the magnitude of this was comparable to that of the commercial infusion 2(C.I.2),‘‘te´de la belleza ”.This tea also had an antioxidant activity comparable to green tea.Ó2007Published by Elsevier Ltd.Keywords:Antioxidant activity;Antimicrobial;Bovine serum albumin;Emulsions;Polyphenols;Tea1.Introduction 1.1.GeneralThe importance of diet for the prevention of some dis-eases is well recognized (Sur,Chandhuri,Vedasiromoni,Gomes,&Ganguly,2001;Wongkham et al.,2001;Yao,Tan,Zhang,Su,&Wei,1998)Antioxidant components are most important in foods because of their ability to reduce free radical-mediated degradation of cells and tis-sues in an organism (Jin,Hakamata,Takahashi,Kotani,&Kusu,2004;Wongkham et al.,2001).Vegetables,legumes and whole-grain cereals (Karakaya &Kavas,1999;Nihal,Ahmad,Mukhtar,&Wood,2005)are good sources of antioxidants but there are many other food sources.Herbal infusions (specially tea)are also important sources (Marongiu et al.,2004;Wu,Ng,&Lin,2004).The average estimated consumption of tea in the United King-dom is 1l/person/day (Karakaya &Kavas,1999;Yana-gimoto,Ochi,Lee,&Shibamoto,2003).Black tea is the most popular drink in the West,and the consumption of green tea is less (18–20%).Less than 2%of tea consumed corresponds to oolong tea,which is very common in China and Taiwan (Karakaya &Kavas,1999;Wheeler &Wheeler,2004;Yao et al.,1998;Yen &Chen,1995).Many investigators have studied tea properties,especially their health-related properties,including antimicrobial and0308-8146/$-see front matter Ó2007Published by Elsevier Ltd.doi:10.1016/j.foodchem.2007.10.040*Corresponding author.E-mail address:m.h.gordon@ (M.H.Gordon)./locate/foodchemAvailable online at Food Chemistry 108(2008)55–63Food Chemistryantioxidant effects,either in homogeneous solution or in biphasic lipid.(Frei&Higdon,2003;Karakaya&Kavas, 1999;Nihal et al.,2005).One of the most important beneficial effects of tea is the antioxidant activity and free radical-scavenging ability of the polyphenol components(Frei&Higdon,2003).Polyphenols are the most important constituents of tea leaves(Karakaya&Kavas,1999;Nihal et al.,2005).Fresh green tea leaves are rich in monomericflavanols,known as catechins(Chattopadhyay et al.,2004;Frei&Higdon, 2003;Lau,He,Dong,Fung,&But,2002;Wheeler& Wheeler,2004)and(À)-epigallocatechin gallate(EGCG) is the most abundant green tea catechin(Frei&Higdon, 2003;Rietveld&Wiseman,2003).Its chemical structure is shown in Fig.1.Catechins are present at levels of30–40% of the dry weight of fresh green tea leaves(Karakaya& Kavas,1999;Nihal et al.,2005;Wheeler&Wheeler,2004).Tea composition is affected by the fermentation process. There are three kinds of teas:not fermented(green and white tea),partially fermented(red and oolong tea)and completely fermented(black tea).During fermentation of fresh tea leaves,some catechins are oxidized or condensed to larger polyphenolic molecules(dimer or polymer)such as theaflavins(3–6%)and thearubigins(12–18%).These polymers are responsible for black tea’s bitter taste and dark colour(Chattopadhyay et al.,2004;Karakaya& Kavas,1999;Nihal et al.,2005;Pelillo et al.,2004;Rietveld &Wiseman,2003;Wheeler&Wheeler,2004).One of the mechanisms of antioxidant action(which is believed to be important in the activity of tea catechins) is to scavenge free radicals,thereby inhibiting oxidation. This mechanism is also the explanation for antimicrobial activity in cells and cell membranes(Frei&Higdon,2003).The antioxidant effectiveness depends on the tea variety and the content of EGCG is very important.Oolong and green tea has high levels of EGCG and(À)-epigallocate-chin(EGC),but the content in black tea is much lower (Katalinic,Milos,Kulisic,&Jukic,2006).The antioxidant abilities of catechins,assessed by the diph-enylpicrylhydrazyl(DPPH)method,were found to be: EGCG>(À)-epicatechin gallate(ECG)>EGC>(À)-epi-catechin(EC)>catechin(C)(Katalinic et al.,2006;Luczaj &Skrzydlewska,2005).1.2.Antimicrobial activityMost polyphenols also show antimicrobial activity. Some studies have investigated the effects of polyphenols on intestinal pathogens,although there is some disagree-ment over precisely which bacterial species are inhibited by antioxidants.Catechins,the polyphenols in tea,pro-anthocyanidins and hydrolysable tannins show antimicro-bial activity.The tolerance of bacteria to polyphenols depends on the bacterial species and the polyphenol struc-ture(Campos,Couto,&Hogg,2003;Taguri,Tanaka,& Kouno,2004).Polyphenols can inhibit growth ofclostridia Fig.1.The structures of the main catechins occurring in Camelia Sinensis and polyphenols in Rooibos.56M.P.Almajano et al./Food Chemistry108(2008)55–63and Helicobacter pylori but not of some intestinal lactic bacteria(Gramza&Korczak,2005).The use of natural antioxidants as preservatives in food has great potential because consumers request additive-free,fresher and more natural-tasting food.However,it is necessary to maintain microbiological safety and mini-mize the number of food-borne microorganisms.In this respect,there are studies that show that tea extracts act as inhibiters of food pathogens,including Staphylococcus aureus,Shigella disenteriae,Vibrio cholerae,Campylobacter jejuni,Listeria monocytogenes,etc.(Negi,Jayaprakasha,& Jena,2003;Taguri et al.,2004).However,not all catechins from tea extracts have antibacterial activity.Some workers have found that green tea extract was not effective against Escherichia coli,although EGCG at10–100l M reduced growth by approximately50%(Nazer,Kobilinsky,Tholo-zan,&Dubois-Brissonnet,2005).1.3.Activity against lipid systemsCatechins scavenge free radicals generated in an aqueous environment preventing them from interacting with lipids in a membrane(Shimada et al.,2004).However,although antioxidants have been frequently studied in oils,emulsions and foods,such as meat,there have been few reports of how proteins,which are commonly present,may affect the activ-ity of antioxidants in foods.Most antioxidants of interest for foods have one or more phenolic hydroxyl groups and there are several studies demonstrating that molecules with this structure may bind to proteins.Polyphenols may asso-ciate with proteins through hydrophobic interactions and hydrogen bonding(Oda,Kinoshita,Nakayama,&Kakehi, 1998)and a range of phenolic antioxidants has also been shown to bind to bovine skin proteins(Wang&Goodman, 1999).Plant phenols have been shown to react with whey proteins at pH9(Rawel,Rohn,Kruse,&Kroll,2002), but such a high pH is not commonly encountered in foods. Bovine serum albumin(BSA)is a minor whey protein with molecular weight66kDa.It is well characterized(Carter& Ho,1994;Peters,1985)and is commercially available in high purity.It has surface active properties and has been used to stabilize model food emulsions(Rampon,Lethuaut, Mouhous-Riou,&Genot,2001).It is known that caffeic acid can bind to BSA(Bartolome,Estrella,&Hernandez, 2000)and chlorogenic acid reacts with BSA at alkaline pH to form an adduct(Rawel et al.,2002).Also,in previous studies(Almajano&Gordon,2004),we found a synergistic effect between BSA and antioxidants,which depended on the antioxidant structure.The objective of this work was to estimate the phenolic content,determine the catechin profile and evaluate the antioxidant and antimicrobial activities of different extracts of teas and herbal infusion.This paper describes,also,a study of the influence of bovine serum albumin(BSA)on the effectiveness offive different infusions as antioxidants in model food emulsions and the relationship between this behaviour and the tea composition.2.Materials and methods2.1.Plant materialThirteen kinds of tea were studied.These comprised four pure teas(white,green,red and black),three pure her-bal infusions(peppermint,nettle and rooibos)and six mixed teas.All teas were purchased from a commercial supplier(Sara Lee Southern Europe S.L.,Mollet del Valle´s, Barcelona,Spain).2.2.Chemicals(À)-Epigallocatechin gallate(EGCG)from green tea, 95%,(À)-epicatechin gallate(ECG)from green tea, 98%,(À)-epigallocatechin(EGC)from green tea,95%, (À)-epicatechin(EC),90%,gallic acid(GA)purity not specified,6-hydroxy-2,5,7,8-tetramethylchromane-2-car-boxylic acid(trolox),bovine serum albumin(BSA), 95%,ferric chloride,Tween-20and2,20-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)diammonium salt(ABTS) were purchased from Sigma–Aldrich Company Ltd.(Gill-ingham,UK).Refined sunflower oil of a brand known to lack added antioxidants was purchased from a local retail outlet.2.3.Microorganism strainsThe microorganism strains used in this study were Bacillus cereus(CECT5144),Micrococcus luteus(CECT 5863),E.coli(CECT99),Pseudomonas aeruginosa(CECT 108),Lactobacillus acidophilus(CECT362)and Candida albicans(CECT1002).They were obtained from the Culture Collection and Research Centre,University of Valencia,Spain.2.4.Infusion preparationEach plant(1.5g)was mixed with100ml of boiling water for5min,with constant shaking and the samples were thenfiltered through Whatman No.1filter paper (WhatmanÒSchleicher&SchuellÒ,Castelldefels,Spain).Infusions for the TEAC and TP assays were treated in three different ways:1.Direct extraction to obtain the infusion.2.Freeze-drying the direct infusion and keeping the solidatÀ20°C until analysis.3.Freezing(À20°C)the direct infusion and analyzingafter2–5weeks.For each procedure,every experiment was performed in duplicate,that is six measures for each infusion.Analysis of samples that had been previously packed and unpacked, were compared with samples that were freshly packed.The results from these three sets are treated together,because significant differences were not found.M.P.Almajano et al./Food Chemistry108(2008)55–63572.5.Determination of antimicrobial activityFor microbiological tests,the infusion was concentrated to afifth of the volume on a rotary evaporator.The prepared samples werefiltered through0.45l m membranes and kept in small sterile bottles and stored atÀ20°C prior to analysis.All microorganisms were grown at30°C for18h in nutrient broth,except Lactobacillus which was grown in MRS for48h.Suspensions of microorganisms,adjusted to4–5log cfu/ml,were placed inflasks containing5ml of sterile nutrient or MRS agar at45–46°C.The mix was poured into Petri plates with10ml of basal medium.When the agar was solidified,sterile discs(6mm)were inserted into the plates.Finally,50l l of solution of each extract were transferred to the discs.The plates were incubated at30°C for48h and read at8,24and48h.At the end of the incubation period,inhibition zones formed in the medium were measured.All tests were per-formed in triplicate.2.6.Removal of tocopherols from sunflower oilTocopherols were removed from sunflower oil by col-umn chromatography using alumina,as described by Yos-hida(1993).2.7.Preparation of emulsionsOil-in-water emulsions(20.2g)were prepared by dis-solving Tween-20(1%)in water containing lyophilized tea(0.1%in weight,final concentration)and BSA(0%or 0.2%in weight,final concentration).The control was pre-pared in the same way,without tea or BSA.The oil was added dropwise to the aqueous sample cooled in an ice-bath while sonicating for5min in total.2.8.Storage and sampling of emulsionsAll emulsions were stored in triplicate in50ml glass beak-ers in the dark(inside an oven).Two aliquots of each(0.005–0.1g,depending on the extent of oxidation)were removed periodically for determination of the peroxide value(PV).2.9.Analytical methods2.9.1.Peroxide value(PV)PV was determined by the ferric thiocyanate method (Frankel,1998)after calibrating the procedure with a series of oxidised oil samples analyzed by the AOCS Official Method Cd8-53.2.9.2.TEAC assayThe radical-scavenging activity of the infusions was also analyzed by the2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)assay(TEAC).The method used was based on that of Re et al.(1999). 2,20-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)and potassium persulfate (7mM ABTS and 2.45mM potassium persulfate,final concentration)were separately dissolved in water and then, the mixture was made up to volume in a10ml volumetric flask.The mixed solution was transferred to an amber bot-tle,covered with aluminium foil and allowed to stand at room temperature for12–16h in the dark.The ABTSÅ+ solution was diluted with phosphate buffer solution(PBS, pH7.4,1:100)to an absorbance of0.7(±0.02)at734nm in a1cm cuvette equilibrated at30°C.PBS(pH7.4)was used as blank.After mixing,the absorbance at734nm(Hewlett Packard8452A Diode Array Spectrophotometer)was measured immediately, and then every minute for5min.Duplicate determinations were made for triplicate samples(six determinations for each sample).The percentage inhibition was calculated from the absorbance values at5min.The relative change in sample absorbance,D A sample,was calculated according the following equation to correct for the solvent:D A sample¼A t¼0ðsampleÞÀA t¼5ðsampleÞA t¼0ðsampleÞÀA t¼0ðsolventÞÀA t¼5ðsolventÞA t¼0ðsolventÞPercent inhibition values were obtained by multiplying D A sample values by100.The TEAC value(trolox equivalent antioxidant capacity)was determined by comparing the values with the standard calibration curve.2.9.3.Total polyphenol(TP)and catechin contentThe TP content of the samples,determined in duplicate for triplicate samples with the Folin–Ciocalteu assay (scaled down to a10mlfinal volume),was quantified in terms of gallic acid.Determination of individual catechins in the samples was conducted by capillary electrophoresis(CE)(Barroso &van de Werken,1999).A calibration curve for each com-pound was prepared.Analyses were carried out in dupli-cate for triplicate samples.2.10.Statistical analysisData from the PV measurements were plotted against time.The times to reach10meq/kg(PV)were determined for each stored sample.Antioxidant capacities,by the ABTSÅ+test,TP and PV induction times,were analyzed by one-way analysis of variance(ANOVA)to determine the pooled standard deviation.The mean values within each test were compared by a two-sample t-test by using the pooled standard deviation to determine significant differences.3.Results and discussion3.1.Antioxidant activityThe codes used and compositions of the commercial teas are given in Table1.Infusions were prepared from each58M.P.Almajano et al./Food Chemistry108(2008)55–63tea,in duplicate,as fresh infusion,frozen infusion and freeze-dried extract,but the compositions and antioxidant activities were not significantly different for the different treatments,so the total phenolics(by the Folin–Ciocalteu method),TEAC values and the ratios between them are reported as the means of at least six measurements.The results are in Table2.The catechin composition is given in Table3.The total polyphenol content values are consistent with the literature(Peterson et al.,2005).The total polyphenol contents were similar for the green tea and white tea,and the content was lower in the black tea.The TEAC values were also higher in white and green teas than in black tea,though no correlation between the parameters(TP and TEAC value)was found.As for the mixed teas,the polyphenol content was higher in samples C.I.2, C.I.3 and C.I.4and it ranged from1750to2247mg lÀ1.TEAC values were also high for two of these samples(C.I.2.and C.I.3)but not for C.I.4.It is well known that the antioxi-dant capacity is related to the type and concentration of infusion catechins(Atoui,Mansouri,Boskou,&Kefalas, 2005)and the catechins present were very different.C.I.1and C.I.4had the same percentage of black tea (80%)but analysis of the catechins showed that both com-mercial samples(mixed tea)had a lower EGCG content than expected from the percentage of black tea present. This can be due to the fact that,during the process of pack-aging and preparing the infusion,there were more losses with mixed tea than with raw tea,due to the unknown influence of other components.So the polyphenols present would be mainly polymerized catechins,especially theaflav-ins and thearubigins.Green tea contained the most EGCG, which is known to be the most active antioxidant catechin (Atoui et al.,2005)and the level of this catechin in C.I.3., which has an important amount of green tea in the formu-lation(Table1),was also high.The highest TEAC value found was for green tea(Table2).The order found was: green tea>C.I.2>C.I.3>white tea>C.I.4%black tea>C.I.1>red tea>C.I.6.The TEAC values for rooi-Table1Codes and compositions of commercial tea samplesMixed teas Commercial teas%Red tea%Green tea%Black tea%Darjeeling tea%White tea Rooibos Other C.I.1Tea assam8020 C.I.2Tea belleza1010104030a C.I.3Tea citrus7525 C.I.4.Tea classic8020 C.I.5Tea infudefensas100 C.I.6.Tea bienestar8812 Other:elderflower,eucalyptus,thyme,mint,grapefruit aroma,lemon aroma,lemon rind,‘‘escaramujo”,hibiscus,vanilla,cinnamon,cardamom,ginger, lemongrass.a Mainly vanilla and hibiscus.Table2Content of total phenolics(by the Folin–Ciocalteu method),TEAC valueand ratio of these parameters for the studied infusions(means±s.d.,n=6)T.P.A TEAC value B PAC CBlack tea1844±15.7e3771±21.2e 2.0Red tea825±117.2c1215±91.7c 1.5Green tea2083±51.3f6344±72.8h 3.0White tea2180±161.6f,g4546±54.3f 2.1Rooibos881±85.2c746±12.9b0.8C.I.11142±133.7d2488±81.7d 2.2C.I.22247±72.1g5282±110.4g 2.4C.I.31866±79.1e4820±109.9f,g 2.6C.I.4.1751±109.0e3884±75.3e 2.2C.I.5630±52.1b383±69.4a0.6C.I.6.965±75.7c,d1004±37.0c 1.0Peppermint infusion315±57.8a758±22.1b 2.4Nettle infusion234±45.2a170±50.6a0.7Different superscript letters,in each column,express significant(P<0.05)differences among results.A T.P.:total phenolics in mg gallic acid equivalent per liter of infusion.B TEAC value:trolox equivalent capacity(mmol of trolox per liter ofinfusion)determined by the ABTS method.C PAC–phenol antioxidant coefficient,calculated as ratio TEAC/totalphenolics.Table3Content of the main catechins(mg/100g tea leaves)determined by capillary electrophoresis(mean±s.d.,n=6)Caffeine EGC EGCG EC ECG Gallic acid Black tea447±14.594.6±4.8270±2.050.2±2.3215±11.844.3±2.8 Red tea537±20.1––23.8±2.035.8±15.181.5±5.2 Green tea515±17.81028±22.72409±62.0131±4.5368±33.720.3±1.6 White tea676±31.2151±15.21525±113.457.3±2.7301±29.354.8±6.4 C.I.1537±21.951.7±9.184.2±9.945.3±2.938.6±0.433.1±3.2 C.I.2542±17.3329±33.41185±95.766.2±3.2272±19.752.6±4.1 C.I.3563±18.5518±21.9958±41.481.7±9.1172±14.125.2±0.8 C.I.4457±29.240.1±4.799.8±7.627.7±1.391.4±7.741.6±4.2M.P.Almajano et al./Food Chemistry108(2008)55–6359bos,peppermint,C.I.5and nettle extracts were very low, compared with the other samples.There was no linear correlation between total polyphe-nols and TEAC values,which reflects the different activity of the polyphenols present in the samples.Tea‘‘belleza”(C.I.2)had high antioxidant activity due to the high content of white tea(40%)and some green tea(10%)in its composition.The concentrations of EGCG, EC,ECG and EGC were similar to those of white tea.This shows that products prepared from mixtures of white tea and green tea may be similar in composition and antioxi-dant properties to green tea but may have a nicerflavour.The polyphenols contribute to astringency,but they are not responsible for theflavour,although there is evidence that interactions offlavanols in green tea extracts during heat processing and storage cause changes in the tea aroma (Wang,Kim,&Lee,2002).Sunflower oil-in-water emulsions(10%of oil),contain-ing0%or0.1%of freeze-dried tea extract and0%or 0.2%of BSA(both0is the control sample),were incubated at37°C.Oxidation of the oil was monitored by determina-tion of the peroxide value.Pure infusions were used instead of commercial samples,because the aim was to design a better functional food infusion(as antioxidant)and we wanted to know the properties of individual extracts.Pep-permint and nettle were not used because their antioxidant activities were not high.At37°C,in the absence of BSA,the different tea extracts showed significant antioxidant activity,with the order of activity being:white tea<red tea$black tea<rooibos$green tea.In samples containing BSA,the order was:white tea (+BSA)<red tea(+BSA)$rooibos tea(+BSA)<black tea(+BSA)$green tea(+BSA).Combining the data for both groups of samples,the order of stability was:white tea<red tea$black tea$white tea(+BSA)<rooibos$green tea$red tea(+BSA)$rooibos tea(+BSA)<black tea(+BSA)$green tea(+BSA)(Fig.2).The time required for the emulsions to reach a peroxide value(PV)of10meq/kg of emulsion is a suitable measure-ment of the antioxidant activity(Fig.3)and it is clear that BSA itself did not have significant antioxidant activity at this concentration(0.2%).However in the presence of BSA,the stability of the samples containing white tea, red tea,and especially black and green teas,increased strongly with the time to PV=10meq/kg increasing by 83%,65%,194%,>57%,respectively(Table4).For rooibos infusion,there was no increase in stability in the presence of BSA.In the absence of protein,green tea was the most effective antioxidant(although not significantly different from rooibos).In the presence of BSA,synergy with some components of the infusions caused the green tea extract to be a stronger antioxidant than rooibos infusion(Table4). There was no significant difference in stability between the samples containing the rooibos infusion,in the presence or absence of BSA.There was a strong synergistic increase in stability in samples containing black tea and BSA,and green tea and white tea also showed some synergy with the protein.This different behaviour is due to the phenolic compo-nents present in the tea extracts.Rooibos infusion does not contain catechins,but contains several compounds, including aspalathin(Fig.1),isoorientin,orientin and rutin (mainly)and others(in lower concentrations),namely iso-vitexin,vitexin,isoquercitrin and hyperoside,quercetin, luteolin and chrysoeryol,all with antioxidant activity.3.2.Antimicrobial activityTable5shows the antimicrobial activities of different teas and infusions.The inhibition zones formed depend on the strain,the kind of extract and the concentration60M.P.Almajano et al./Food Chemistry108(2008)55–63of extract.Probably a large concentration or volume of infusion added could provide the greatest effect but the lim-ited capacity of the discs did not allow paring the five bacteria studied,it is clear that the strain B.cereus is most sensitive,showing the largest inhibition diameter in the presence of the tea extracts.Extracts from all theteasFig.3.Times for emulsions to reach PV =10meq/kg with and without BSA.Table 4Times (in days)for oil-in-water emulsions stored at 37°C to reach PV =10meq/kg,and synergy between BSA and the infusion calculated from this dataWithout BSAWith BSA %Synergy Control 1.61 2.21–Black tea 25.0372.15194Red tea 25.3341.7465.0Green tea 47.64>75>57.0White tea 15.9228.9383.0Rooibos43.6443.34Values with the same superscript number are not significantly different (P <0.05)(mean for triplicate samples).The induction period (IP)was calculated as the time (in days)required to reaching PV =10meq/kg sample (Table 4).Synergy was calculated from theinduction period,by modifying the equations of Satue ´-Gracia,Heinonen,and Frankel (1997)and Alaiz,Hidalgo,and Zamora (1997):%synergism ¼100½IP ðantioxidant þprotein ÞÀIP ðcontrol Þ À½ðIP antioxidant ÀIP control ÞþðIP protein ÀIP control Þ½ðIP antioxidant ÀIP control ÞþðIP protein ÀIP control Þ:Table 5Effect of extract infusions on antimicrobial activity (diameter of the inhibition zone measured in mm)Bacillus cereus Micrococcus luteus Pseudomonas aeruginosa Escherichia coli Lactobacillus acidophilus Candida albicans Averages.d.Average s.d.Average s.d.Average s.d.Average s.d.Average s.d.White tea 12.50.0612.40.28 6.70.03 6.40.03––8.50.03Green tea 13.90.0811.20.1610.80.17 6.20.02––8.00.09Red tea 7.70.097.10.04–0.00–––– 6.70.12Black tea 12.00.1010.00.0011.30.06––––––C.I.18.70.29 6.70.067.70.12––––––C.I.214.00.109.00.2010.30.297.00.00––7.70.09C.I.314.70.0613.30.1512.00.17––––7.00.00C.I.49.70.237.70.129.00.17––––––C.I.57.00.007.00.00––––––––C.I.67.00.00––––––––––Rooibos7.00.096.40.03––––––8.50.22–indicates no inhibition (s.d.standard deviation for n =3).M.P.Almajano et al./Food Chemistry 108(2008)55–6361studied had an inhibitory effect against this strain.The sec-ond most sensitive strain was M.luteus,followed by P. aeruginosa.In contrast E.coli was only inhibited very weakly for some of the extracts studied(white,green tea and C.I.2).In general,Gram negative bacteria are more resistant to polyphenols than Gram positive bacteria,per-haps due to the different cell wall compositions(Negi et al.,2003).L.acidophilus showed exceptional resistance to all extracts studied.The results obtained are very inter-esting because the tea extracts inhibit the food-borne bac-teria but not the intestinal bacteria studied.The yeast C. albicans was inhibited by extracts from green and white tea and from some commercial teas(C.I.2and C.I.3).This yeast was more resistant than were the strains of Bacillus, Micrococcus and Pseudomonas assayed.The antimicrobial activity is higher in non-fermented tea than in semi-fermented or fermented tea.The highest anti-microbial activity corresponds to the highest antioxidant activity(TEAC values)and less to total polyphenol con-tent.For this reason we can claim that not all tea’s poly-phenols have antimicrobial effects.Our results are in agreement with those of Gramza and Korczak(2005), who studied the effects of individual catechins separately and found that EGCG and EGC are those with highest antioxidant and antimicrobial powers.Our experiments showed that white and green tea extracts and the commer-cial teas C.I.2and C.I.3were the best microbiological inhibitors.They are also the extracts that have the highest EGCG and EGC concentrations.These extracts contain the catechins EGCG+EGC with the sum of the concen-trations being higher than1400mg/100g tea leaves.In contrast,semi-fermented and fermented teas have lower concentrations of these catechins.The contents of both EGCG and EGC were less than365mg/100g tea leaves and the antimicrobial activities of these components were less too.The use of extracts from green and white teas,in combi-nation with other antimicrobial components or methods for stabilizing food products,is an alternative way of main-taining a highflavour quality(Nazer et al.,2005).The peppermint and nettle infusions did not have anti-microbial activity.These results are consistent with the low total polyphenol content and,moreover,low antioxi-dant capacity in the nettle infusion.These infusions seem to have insufficient activity to justify their use as natural preservatives in food.However,the rooibos infusion had a low antimicrobial activity against B.cereus.Rooibos does not contain catechins,but it contains various others compounds(aspalathin,isoorientin,orientin and rutin) that could contribute to the antioxidant and antimicrobial activities.4.ConclusionsThere is no significant effect of packaging and dry stor-age time on the contents of total phenolic compounds and on the antioxidant activity.The phenols present in rooibos infusion have a strong antioxidant activity in model food emulsions,but their activity in retarding oxidation of oil droplets in oil-in-water emulsions does not increase in the presence of BSA so there is no synergistic effect.The antimicrobial activity of non-fermented tea is higher than that of semi-fermented or fermented tea.The highest antimicrobial activity occurs in samples with the highest total polyphenol concentration and antioxidant activity. White and green teas and commercial teas,like C.I.2and C.I.3,are the best sources of extracts which can act as microbiological inhibitors.The use of these teas in combi-nation with other antimicrobial additives or methods for stabilizing food products represents an alternative way of maintaining a highflavour quality without the use of con-ventional food preservatives.AcknowledgmentThis work was supported by Sara Lee Southern and by ‘‘Ministerio de Ciencia y Tecnologı´a”of Spain with the project AGL2005-08088-C02-02.The authors thank Judit Pe´rez Gonza´lez and M.Eugenia Delgado for technical support.ReferencesAliaz,M.,Hidalgo,F.J.,&Zamora,R.(1997).Comparative antioxidant activity of Maillard and oxidized lipid-damaged bovine serum albu-min.Journal of Agricultural and Food Chemistry,45,3250–3254. Almajano,M.P.,&Gordon,M.H.(2004).Synergistic effect of BSA on antioxidant activities in model food emulsions.Journal of the American Oil Chemists Society,81(3),275–280.Atoui,A.K.,Mansouri,A.,Boskou,G.,&Kefalas,P.(2005).Tea and herbal infusions:Their antioxidant activity and phenolic profile.Food Chemistry,89(1),27–36.Barroso,M.B.,&van de Werken,G.(1999).Determination of green and black tea composition by capillary electrophoresis.HRC–Journal of High Resolution Chromatography,22(4),225–230.Bartolome,B.,Estrella,I.,&Hernandez,M.T.(2000).Interaction of low molecular weight phenolics with proteins(BSA).Journal of Food Science,65(4),617–621.Campos,F.M.,Couto,J.A.,&Hogg,T.A.(2003).Influence of phenolic acids on growth and inactivation of Oenococcus oeni and Lactobacillus hilgardii.Journal of Applied Microbiology,94(2),167–174.Carter,D.C.,&Ho,J.X.(1994).Structure of serum-albumin.Advances in Protein Chemistry,45,153–203.Chattopadhyay,P.,Besra,S.E.,Gomes,A.,Das,M.,Sur,P.,Mitra,S., et al.(2004).Anti-inflammatory activity of tea(Camellia sinensis)root extract.Life Sciences,74(15),1839–1849.Frankel,E.(1998).Lipid oxidation.Dundee:Oily Press.Frei,B.,&Higdon,J.V.(2003).Antioxidant activity of tea polyphenols in vivo:Evidence from animal studies.Journal of Nutrition,133(10), 3275S–3284S.Gramza,A.,&Korczak,J.(2005).Tea constituents(Camellia sinensis L.) as antioxidants in lipid systems.Trends in Food Science and Technol-ogy,16(8),351–358.Jin,D.,Hakamata,H.,Takahashi,K.,Kotani,A.,&Kusu,F.(2004).Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochem-ical detection(vol.18,p.662,2004).Biomedical Chromatography, 18(10),876.Karakaya,S.,&Kavas,A.(1999).Antimutagenic activities of some foods.Journal of the Science of Food and Agriculture,79(2),237–242.62M.P.Almajano et al./Food Chemistry108(2008)55–63。

泽漆醇提取物抗氧化活性及对油酸诱导HepG2细胞脂肪堆积的影响

甘露珍,姜琼,饶志威,等. 泽漆醇提取物抗氧化活性及对油酸诱导HepG2细胞脂肪堆积的影响[J]. 食品工业科技,2024,45(6):330−336. doi: 10.13386/j.issn1002-0306.2023050131GAN Luzhen, JIANG Qiong, RAO Zhiwei, et al. Antioxidant Activity of Euphorbia helioscopia Ethanol Extract and Its Effect on Oleic Acid Induced Fat Accumulation in HepG2 Cells[J]. Science and Technology of Food Industry, 2024, 45(6): 330−336. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050131· 营养与保健 ·泽漆醇提取物抗氧化活性及对油酸诱导HepG2细胞脂肪堆积的影响甘露珍,姜 琼,饶志威,张聪子,杜 光,章登政*(咸宁市中心医院/湖北科技学院附属第一医院药学部,湖北咸宁 437199)摘 要:为了探究泽漆醇提取物体外抗氧化作用及其对油酸诱导HepG2细胞脂肪堆积的影响。

本研究采用浸渍法分别以25%、50%、75%甲醇和乙醇对泽漆粉末进行提取,评估不同泽漆提取物的体外抗氧化活性,并测定其总酚和总黄酮含量。

建立HepG2细胞脂肪堆积模型,不同浓度(20、40、60 μg/mL )泽漆50%乙醇提取物处理24 h ,观察细胞内脂滴形成情况;测定细胞中甘油三脂(triglyceride ,TG )含量、总谷胱甘肽(glutathione ,GSH )、超氧化物歧化酶(superoxide dismutase ,SOD )的活性及总抗氧化能力(total antioxidant capacity ,T-AOC )。

ariix爱睿希超级水果素


卡瓦椒
逾百年来,卡瓦椒的放松作用一直被受重视。卡瓦椒原产自西太平洋,波利尼西亚岛民 习惯咀嚼该植物达致镇静和麻醉的功效,卡瓦椒能够舒缓情绪、放松肌肉,而不会影响 头脑清晰。德国科学家早在1890年已使用卡瓦椒来降低血压。跟红酒一样,各种卡瓦椒 不尽相同,不同国家的卡瓦椒强度不一,其内酯含量和质量亦有分别。ARIIX 超级水果
超级水果素
能量充沛 表现提升
蕴含多种超级水果萃取物及抗养化剂,有助补充能量、增强体力
我们日常饮食所摄取的营养,对我们的生活能够产生深远的影响。节奏急促、忙碌的生活方式能迅速令 人精神萎靡、健康状况衰退。要扭转这局面,营养丰富的饮食和恒常运动是达致身心健康的第一步。 除此之外,你可以服用提升能量、促进健康的营养补充品,并将之成为日常习惯的一部份。
Wang, Mian-Ying; et al (2009). "Antioxidant activity of noni juice in heavy smokers". Chemistry Central Journal 3: 13.
Dharmananda S. Sea buckthorn, Institute of Traditional Medicine Online, 2004.
ARIIX 致力追求卓越质量
ARIIX透过广泛研究现代营养学,致力为您带来顶尖的营养补充品。ARIIX的100%效能保证是我们业务的基石。 除此之外,我们致力于释放人的潜能。
©ARIIX Hong Kong Ltd. 2011
超级水果素
营养补充品数据
每次食用4粒/每瓶含 28 份
每次食用含量
超级水果混合物
Jung HA, Su BN, Keller WJ, Mehta RG, Kinghorn AD (March 2006). "Antioxidant xanthones from the pericarp of Garcinia mangostana (Mangosteen)". Journal of Agricultural and Food Chemistry 54 (6): 2077–82.

自由基清除率英语

自由基清除率英语
"自由基清除率"的英语表达可以是"Free Radical Scavenging Rate"或"Antioxidant Activity",具体取决于上下文和描述的背景。

以下是两个表达的例子:
Free Radical Scavenging Rate:
"The herbal extract demonstrated a high free radical scavenging rate, indicating its potential as a natural antioxidant."
中文翻译:该草药提取物表现出较高的自由基清除率,表明其具有作为天然抗氧化剂的潜力。

Antioxidant Activity:
"Researchers measured the antioxidant activity of various compounds and found that vitamin C exhibited significant antioxidant activity."
中文翻译:研究人员测量了各种化合物的抗氧化活性,发现维生素C表现出显著的抗氧化活性。

这两个表达都涉及到评估物质抵抗自由基损伤的能力,其中"自由基清除率"强调了对自由基的清除,而"抗氧化活性"则更广泛地描述了抗氧化作用。

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REASONS FOR NOT EATING FRUITS AND VEGETABLES
• Reluctance to return unsatisfactory produce associated with a sense of buy responsibility • Emerging competition from the do-it-yourself health-care market and from manufactured functional foods that deliver the health benefits of fresh produce without the mess • More aggressive marketing, advertising and pricing by manufacturers thanprovide 3800, kcal/day for every man, woman, and child • - Twice what women need • - 1/3 more than what most men need • - far higher than what children need
PHYTONUTRIENTS
Fruits, vegetables and herbs contain many different phytonutrients, many of which have antioxidant properties
PHYTONUTRIENTS
• Lower incidence and mortality rates of cancer
Leading Causes of Death in the United States
Rank 1 2 3 4 Cause of Death Diseases of the heart Cancers Strokes Pulmonary diseases % of Total 32 23 7 5
Consumers Want Taste
Leading factor in food purchases: • • Taste Nutrition 91% 79%
4-6 week delay before consumer repuchases produce after bad taste experience
Consumer awareness of foods that may reduce risk of disease
• • • • • • • • • Broccoli Other fruits Oranges/juice Carrots Fish/Fish Oil Garlic Green, leaf vegetables Milk Fiber 21% 20% 13% 10% 10% 10% 9% 8% 7%
Most Healthful Effects
• Americans’ belief that the following foods have a “very healthful effect” - 92% Fruit and vegetable - 62% Dried beans - 61% Grain foods - 57% Fish - 55% Poultry - 37% Dairy
Background on Functional Foods
“Functional Foods” are foods that may provide a health benefit beyond basic nutrition. The good news with functional foods is that what you do eat may be more important for your health than what you don’t eat. Examples include everything from fruits and vegetables to fortified or enhanced foods. Functional attributes of many traditional foods are being discovered, while new food products are being developed with beneficial components.
Consumers Watch Diet
• Shoppers take some sort of dietary action to ensure their diet is healthy • Fruits and vegetables top the list as the key to a healthy diet (71%). Other dietary actions include eating fewer fats and oils, meat and snack or junk food
and atherosclerosis(动脉硬化症) • Lower ischemic heart disease mortality • Reduce blood pressure, inflammation(炎症) • Lower cardiovascular(心脏血管的 )and cerebrovascular (脑血管的)disease mortality • Boost immune system, forestall aging • Prevent some loss of long-term memory • Detoxify contaminants and pollutants
Consumers Link Foods with Health Benefits • Consumer participants expect to find functional foods in their regular supermarket, distributed throughout the store • Consumer participants said they preferred to receive functional food components from natural sources, but expressed comfort with fortification and agreed that supplements, such as vitamins, are acceptable because there is not always time to eat the “right” foods
5
6
Accidents
Pneumonia & Influenza
4
4
7
Diabetes mellitus
3
Fruits and Vegetables Play Preventative Role in…….
• • • • • • Cancer Heart Disease Stroke Hypertension Birth Defects Cataracts • • • • • Diverticulosis Diabetes Mellitus Osteoporosis Obesity Chronic Obstructive Pulmonary Disease (COPD)
REASONS FOR NOT EATING FRUITS AND VEGETABLES
• They don’t know how • There is too much work, time, mess or thinking/learning involved • A combination of the first two • Price and affordability, especially for fruits • Taste and spoilage risks associated with buying produce • Concerns and fears about pesticide residues
Consumers Link Foods with Health Benefits
• 85% know calcium and dairy products are associated with a decreased risk of osteoporosis • 56% know carrots are associated with eye disease and vision deterioration • 47% correctly associate cranberries and cranberry juice with a lower risk of urinary tract infection
Antioxidant Capacity of Fruits, Vegetables and Herbs
Shiow Y. Wang
Fruit Laboratory, ARS, USDA,
Beltsville, MD, U. S. A.
In the past, the agricultural industry was focused on maximizing the quantity of fruits and vegetables produced for commercial markets. However, modern consumers are now interested in optimizing the nutritional composition of foods in order to promote health. Therefore, much attention has now been placed on the agricultural practices which will enhance the nutritional content of fruits and vegetables being produced today
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