大鼠(GDNF)ELISA试剂盒说明书

大鼠(GDNF)ELISA试剂盒说明书
96T/48T
大鼠(GDNF)ELISA试剂盒说明书
大鼠(GDNF)ELISA试剂盒说明书Preparation of reagents
Standard products: series of dilution of the standard product should be prepared in the experiment, can not be stored. Before dilution, the standard product is mixed evenly.
2 washing buffer solution (50 *) dilution: distilled water 50 times dilution.
Operation steps
1 before use, all reagents fully mixed. Do not allow liquid to produce a large number of bubbles, so as to avoid adding a large number of bubbles, resulting in the addition of the error.
2 according to the number of samples to be measured and the number of standard products to determine the number of required. Each standard and blank hole is recommended to do the hole. Each sample can be made according to its own quantity, and can be used as a hole in the hole.
3 to join the dilution of the standard 50ul in the reaction hole, to add the sample to be measured in the reaction hole 50ul. The biotin labeled antibody was immediately added to the 50ul. Cover the membrane plate, gently oscillating mixing, 37 degrees Celsius incubation 1 hours.
4 left hole liquid, each hole filled with washing liquid, oscillating 30 seconds off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If the washing machine with washing, washing times increased once.
5 each hole to join 80ul affinity chain enzyme -HRP, gently oscillating mixing, 37 degrees Celsius incubation 30 minutes.
6 left hole liquid, each hole filled with washing liquid, oscillating 30 seconds off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If the washing machine with washing, washing times increased once.
7 each hole to join the substrate A, B each 50ul, gently oscillating mixing, 37 degrees Celsius incubation 10 minutes. Avoid light.
8 remove the enzyme labeled plate, quickly join the 50ul terminator, add the end of the liquid should be measured immediately after the results.
9 OD value at the wavelength of 450nm was measured by hole.
limit
The result of the above 6 standard is nonlinear, and the result can not be obtained accurately according to the standard curve.
本试剂仅供研究使用标本：全血
试验原理：
HLA- B27试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知HLA-B27浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。

大鼠(GDNF)ELISA试剂盒说明书先将HLA- B27和生物素标记的抗体同时温育。

洗涤后，加入亲和素标记过的HRP。

再经过温育和洗涤，去除未结合的酶结合物，然后加入底物A、B，和酶结合物同时作用。

产生颜色。

颜色的深浅和样品中HLA-B27的浓度呈比例关系。

试剂盒组成：
大鼠(GDNF)ELISA试剂盒说明书样品收集、处理及保存方法
1、血清-----操作过程中避免任何细胞刺激。

使用不含热原和内毒素的试管。

收集血液后，1000×g 离心10分钟将血清和红细胞迅速小心地分离。

2、血浆-----EDTA、柠檬酸盐、肝素血浆可用于检测。

1000×g离心30分钟去除颗粒。

3、细胞上清液---1000×g离心10分钟去除颗粒和聚合物。

4、保存------如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。

尽可能的不要使用溶血或高血脂血。

如果血清中大量颗粒，检测前先离心或过滤。

不要在124℃或更高的温度加热解冻。

应在室温下解冻并确保样品均匀地充分解冻。

Sample collection, processing and storage method
1, serum - the operation of the process to avoid any cell stimulation. Pyrogen and endotoxin free tube. After collecting blood, 1000 * g centrifugation for 10 minutes, the serum and red blood cells were separated rapidly and carefully.
2, plasma -----EDTA, citrate, heparin plasma can be used to detect. 1000 * g centrifugal 30 minutes to remove particles.
3, cell supernatant ---1000 * g centrifugation 10 minutes to remove particles and polymers.
4, save - if the sample is not immediately used, it should be divided into a small part of -70 degrees C to avoid repeated freezing. As far as possible, do not use the blood of hemolysis or high blood fat. If a large number of particles in the serum, before the detection of centrifugal or filtration. Do not heat the thaw at 124 or higher. Should be thawed at room temperature and to ensure that the samples are uniformly charged and frozen.
大鼠(GDNF)ELISA试剂盒说明书Kit composition
130 times concentrated washing liquid 20ml * 1 bottles; 2 enzyme labeled reagent 6ml * 1 bottle
3 enzyme labeled plate 8 hole * * 12;
4 sample dilution 6ml * 1 bottle
5 color reagent A liquid 6ml * 1 bottle;
6 color agent B liquid 6ml * 1/ bottle
7 termination solution 6ml * 1 bottle;
8 standard product (48ng/ml) 0.5ml * 1 bottle
9 standard 1.5ml * 1 bottle;
10 Manual 1 copies
11 plate film 2; 12 sealing bag 1
供应商：上海乔羽生物有限公司。