一种新组织工程方法桥接修复缺损坐骨神经

一种新组织工程方法桥接修复缺损坐骨神经
化学脱细胞同种异体神经不但去除了许旺细胞、髓鞘及其崩解碎片等可引起排斥反应的物质，减轻了移植修复后的免疫排斥反应，同时也保留了为缺损神经修复再生过程中提供良好再生支架的神经基质管，具有与自体神经移植近似的引导神经再生的功能，为神经再生提供良好的局部微环境。

来自中国郑州大学国际教育学院的张雁儒团队，设想用大鼠双侧坐骨神经制备化学去细胞同种异体神经，并联合脑源性神经营养因子复合骨髓间充质干细胞桥接修复大鼠坐骨神经1 0mm缺损后发现，大鼠的坐骨神经指数、三头肌湿质量恢复率、髓鞘厚度、有髓神经纤维数量在3组中的变化为：脱细胞同种异体神经+脑源性神经营养因子+间充质干细胞组＞脱细胞同种异体神经+间充质干细胞组＞脱细胞同种异体神经组。

修复效果优于单独应用化学脱细胞同种异体神经桥接和脱细胞同种异体神经复合骨髓间充质干细胞移植。

文章发表在《中国神经再生研究（英文版）》杂志2014年10月第20期。

Article: " Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone," by Yanru Zhang1, 2, Hui Zhang3, Gechen Zhang3, Ka Ka1, Wenhua Huang2 (1 School of International Education, Zhengzhou University, Zhengzhou, Henan Province, China; 2 Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China; 3 Department of Orthopedic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China)
Zhang YR, Zhang H, Zhang GC, Ka K, Huang WH. Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone. Neural Regen Res. 2014;9(20):1814-1819.
欲获更多资讯：Neural Regen Res/
A novel tissue engineering method for repair of sciatic nerve defects
Chemically extracted acellular nerve allografts can eliminate Schwann cells, myelin and disintegrating debris that can cause rejection, attenuate immune rejection after transplantation, and also help retain the nerve canal. These canals can provide a good scaffold for the regeneration of nerve defects, and have a similar effect to autologous nerve transplantation in guiding nerve regeneration, offering a local environment for nerve recanalization. Yanru Zhang at School of International Education, Zhengzhou University, China and his colleagues chemically extracted acellular nerve allografts from bilateral sciatic nerves, and repaired 10-mm sciatic nerve defects in rats using these grafts and brain-derived neurotrophic factor (BDNF) transfected bone marrow mesenchymal stem cells (BMSCs). Sciatic functional index, triceps wet weight recovery rate, myelin thickness, and number of myelinated nerve fibers were significantly changed in the acellular nerve allograft group, acellular nerve allograft + BMSCs group, and acellular nerve allograft + BDNF transfected BMSCs group. Variations were the largest in the acellular nerve allograft + BDNF transfected BMSCs, followed by acellular nerve allograft + BMSCs group, and lastly the acellular nerve allograft group. Experimental findings suggest that chemically extracted acellular nerve allograft combined with nerve factor and mesenchymal stem cells can promote the restoration of sciatic nerve defects. The repair effect of acellular nerve allograft + BDNF transfected BMSCs is superior to single application of acellular nerve allograft or acellular nerve allograft combined with BMSCs transplantation. This article has been published in Neural Regeneration Research (Vol. 9, No. 20, 2014).
Article: " Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone," by Yanru Zhang1, 2, Hui Zhang3, Gechen Zhang3, Ka Ka1, Wenhua Huang2 (1 School of International Education, Zhengzhou University, Zhengzhou, Henan Province, China; 2 Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China; 3 Department of Orthopedic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China)
Zhang YR, Zhang H, Zhang GC, Ka K, Huang WH. Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone. Neural Regen Res. 2014;9(20):1814-1819.。