5(6)-TAMRA SE_246256-50-8_DataSheet_MedChemExpress
RayBio
RayBio® Mouse RANTES IQELISAKitCatalog #: IQM-RANTESUser ManualLast revised August 23, 2021Caution:Extraordinarily useful information enclosedISO 13485 Certified3607 Parkway Lane, Suite 100Norcross, GA 30092 Tel: 1-888-494-8555 (Toll Free) or 770-729-2992, Fax:770-206-2393Web: , Email: *******************RayBiotech, Inc.________________________________________RayBio® Mouse RANTES IQELISA Kit ProtocolTable of ContentsSection Page # I.Introduction3II.Reagents3III.Storage3IV.Additional Materials Required4V.Reagent Preparation4VI.Assay Procedure5VII.Assay Procedure Summary7VIII.Calculation of ResultsA. Typical DataB. Sensitivity and Recovery 8 9 9IX.Troubleshooting Guide10I. INTRODUCTIONThe RayBio®I mmuno Q uantitative E nzyme L inked I mumuno S orbent A ssay (IQELISA) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio® Mouse RANTES IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Mouse RANTES in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Mouse RANTES coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. C t values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower C t values indicate a higher concentration of antigen.II. REAGENTS1.RANTES PCR Plate: 96-well PCR plate coated with anti-Mouse RANTES2.PCR Plate film3.Wash Buffer I Concentrate (20x): 25 ml of 20x concentrated solution4.Standards: 2 vials of recombinant Mouse RANTES5.Assay Diluent A: 30 ml diluent buffer, 0.09% sodium azide as preservative.6.Assay Diluent B: 15 ml of 5x concentrated buffer.7.Detection Antibody for RANTES: 2 vials of a concentrated solution of anti-MouseRANTES affinity reagent8.IQELISA Detection Reagent: 1.4ml of a 10x concentrated stock9.Primer Solution: 1.7ml vial10.PCR Master Mix: 1.2ml vial11.PCR Preparation buffer: 1ml vial of 10x concentrated buffer12.Final Wash Buffer: 10 ml vial of 10x concentrated bufferIII. STORAGEMay be stored for up to 6 months at 2°to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.IV. ADDITIONAL MATERIALS REQUIRED1.Real-time PCR instrument, Bio-Rad recommended2.Precision pipettes to deliver 2µl to 1 ml volumes.3.Adjustable 1-25 ml pipettes for reagent preparation.4.100 ml and 1 liter graduated cylinders.5.Absorbent paper.6.Distilled or deionized water.7.Log-log graph paper or computer and software for data analysis.8.Tubes to prepare standard or sample dilutions.9.Heating block or water bath capable of 80°CV. REAGENT PREPARATION1.Bring wash buffer, samples, assay diluents, and PCR plate to room temperature (18 - 25°C) before use. PCR master mix and Primer solution should be kept at 4°C at all times.2.Sample dilution: If your samples need to be diluted, 1x Assay Diluent B should be usedfor dilution of serum/plasma samples. Assay Diluent A maybe used in place if significant matrix affects are seen.Suggested dilution for normal serum/plasma: 20 fold*.*Please note that levels of the target protein may vary between different specimens.Optimal dilution factors for each sample must be determined by the investigator.3.Assay Diluent B should be diluted 5-fold with deionized water.4.Briefly spin the Detection Antibody vial before use. Add 25 µl of 1X Assay Diluent B intothe vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). This concentrate should be diluted 80-fold with 1X Assay Diluent B and used in step 4 of the Assay Procedure.5.PCR preparation buffer should be transferred to a 15mL tube and diluted with 9mL ofdeionized or distilled water before use.6.Final Wash Buffer should be transferred to a 15mL tube and diluted with 9mL ofdeionized or distilled water for every 1mL of 10x concentrate used before use.7.Preparation of standard: Preparation of standard: Briefly spin a vial of Standard. Add400 µl 1x Assay Diluent into the vial of Standard to prepare a 50 ng/ml standard.Dissolve the powder thoroughly by a gentle mix. Add 4 µl RANTES standard (50 ng/ml) from the vial of Standard, into a tube with 996 µl 1x Assay Diluent B to prepare a 200 pg/ml standard solution. Pipette 300 µl 1x Assay Diluent B into each tube. Use the 200 pg/ml standard solution to produce a dilution series (shown below). Mix each tubethoroughly before the next transfer. 1x Assay Diluent B serves as the zero standard (0 pg/ml).996 µl + 4 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl100 µl+ 300 µl2000 pg/ml500pg/ml125pg/ml31.25pg/ml7.813pg/ml1.953pg/ml0.488pg/mlpg/ml8.If the Wash Buffer Concentrate (20x) contains visible crystals, warm to roomtemperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.9.Prepare the IQELISA detection reagent by calculating how much will be needed. Thismay be accomplished by multiplying the number of wells to be assayed by the volume you plan to use per well. Once the volume of IQELISA detection reagent is known,prepare the reagent by diluting it 1:10 with deionized water and mixing thoroughly.VI. ASSAY PROCEDURE1.Bring all reagents and samples to room temperature (18 - 25°C) before use. It isrecommended that all standards and samples be run in triplicate. Partial plate runs may be accomplished by cutting the PCR plate into the desired number of strips using a pair of sturdy scissors, wire cutters, or shears. The remainder may be saved and used for a later date. If this is done, the PCR Plate Film should also be cut to a suitable size.2.Add 10-25µl of each standard (see Reagent Preparation step 2) and sample intoappropriate wells. Volumes should be consistent between all wells, samples, andstandards. As little as 10µL can be used if sample volume is limited, however thisincreases the chance of technical error. Ensure there are no bubbles present at thebottom of the wells. Dislodge any bubbles with gentle tapping or with a pipette tip being careful not to contact the sides or bottom of the well. Cover well and incubate for 2.5hours at room temperature or overnight at 4°C with gentle shaking.3.Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each wellwith Wash Buffer (100 µl) using a multi-channel Pipette or autowasher. Completeremoval of liquid at each step is essential to good performance. After the last wash,remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.4.Add 25 µl of prepared Detection Antibody (Reagent Preparation step 4) to each well.Incubate for 1 hour at room temperature with gentle shaking.5.Discard the solution. Repeat the wash as in step 3.6.Add 25µL of prepared IQELISA detection reagent and incubate 1 hour with rocking(Reagent Preparation step 9)7.Discard the solution. Repeat the wash as in step 3.8.Add 100µL of Final wash buffer to each well and incubate for 5 minutes with rocking.Remove the solution from each well and repeat an additional 2x.9.Add 100µL of 1x PCR preparation buffer to each well and incubate with rocking for 5minutes before removing the buffer. Blot the plate after the buffer is removed to ensure complete removal of the buffer.10.Add 15µL of the Primer solution to each well of the plate. At this stage the plate can becovered and stored at -20°C for use the next day if needed.11.Add 10µL of PCR Master Mix to each well and pipette thoroughly to mix the well (atleast 3x up and down).12.Cover the plate with the supplied PCR Plate Film, taking care to insure the film iscompletely and even pressed onto the plate, creating an air tight seal around each well of the plate.13.Place the plate into a real-time PCR instrument using a FITC compatible wave length fordetection with the following settings for cycling1.3 minute activation at 95°C2.10 seconds 95°C denaturation3.25 seconds 62°C annealing/extension4.Repeat steps 2 and 3 29xVII. ASSAY PROCEDURE SUMMARY1.Prepare all reagents, samples and standards as instructed.2.Add 25µl standard or sample to each well.Incubate 2.5 hours at room temperature or overnight at 4°C.3.Add 25µl Detection Antibody to each well.Incubate 1 hour at room temperature.4.Add 25µL of IQELISA Detection Reagent to each well. Incubate 1 hour5.Add 15µl Primer solution and 10µL of PCR master mix to each well6.Run real-time PCRVIII. CALCULATION OF RESULTSThe primary data output of the IQELISA kit is C t values. These values represent the number of cycles required for a sample to pass a fluorescence threshold. As the DNA is amplified additional fluorescent signal is produced, with each cycle resulting in an approximate doubling of the DNA. Therefore, higher levels of DNA (directly related to the amount of antigen in the sample) result in lower C t values.Calculate the mean C t for each set of triplicate standards, controls and samples. Subtract the C t value of each sample from the control to obtain the difference between the control and sample (Delta C t). Plot the values of the standards on a graph using a log scale for concentration on the x axis. This graph is the quickest way to visualize results, although not the most accurate. If this method is used the concentration of unknown samples can be estimated using a logarithmic line of best fit.The line of best fit will have an equation y = mln(x)+b, where y is the Delta C t value and x is the concentration. It may be helpful to use 5 significant figures for m and b to minimize rounding errors. To calculate the concentration of unknown sample this can be entered into Excel in the following format=EXP((y-b)/m))Where y is the Delta C t obtained during the assay, and b and m are obtained from the line of best fitAlternatively, for a more accurate representation linear regression may be used. Both the Delta C t and Concentration can be transformed using a log base of 10, plotted on a graph as described above, along with a line of best fit (using a linear model). The equation of this line may be used to calculate the antigen concentration of unknown samples. This is the method used for the analysis spreadsheet for IQELISA available online.A. TYPICAL DATAThese data are for demonstration only. A standard curve must be run with each assay.B. SENSITIVITY and RECOVERYThe minimum quantifiable dose of RANTES is typically 0.48 pg/ml, however levels as lower than 0.48 pg/ml may be detected outside of the quantification range.Serum spike tests show recovery is 97% with a range from 87% to 112%ntraplate CV is below 10% for all samples and Interplate CV is below 15%X. TROUBLESHOOTING GUIDEProblem Cause SolutionPoor standard curve Inaccurate pipettingImproper standard dilutionCheck pipettesBriefly centrifuge standards anddissolve the powder thoroughly bygently mixingLow signal Too brief incubation timesInadequate reagentvolumes or improperdilutionEnsure sufficient incubation time.Assay procedure step 2 may bedone overnightCheck pipettes and ensure correctpreparationLarge CV Uneven pipettingBubbles present in wellsCheck pipettesLightly tap or use pipette tip todislodge from bottom of wellHigh background Plate is insufficientlywashedContaminated washbufferImproper TmReview the manual for proper wash.If using a plate washer, ensure thatall ports are unobstructed.Make fresh wash bufferCheck run parameters and calibrateinstrumentLow sensitivity Improper storage of theIQELISA kitImproper TmStore your standard at <-20°C afterreconstitution, others at 4°C.Check run parameters and calibrateinstrumentThis product is for research use only.©2019 RayBiotech, Inc11。
ATE Corporation AS-05 Antenna Set 30 MHz to 18 GHz
Main Features•30 MHz to 18 GHz frequency range •Excellent Antenna Factor•Tripod adapter for easy vertical-horizontal polarization change •Individual calibration•Robust, rustproof aluminium construction •LightweightAS-05 is a compact size broadband Antenna System composed of a BC-01 Biconical Dipole, LP-04 Log Periodic Dipole Array and DR-01 Double Ridged horn Antenna designed for radiated emissions and immunity testing. It can be used in conjunction with any receiver or spectrum analyzer.Its ideal companion is the EMI Receiver Unit 9060 and 9180 that can be easily mounted on the antenna mast (*).(*)The direct connection between antenna and PMM Receiver Unit eliminates additional sources of uncertainties due to coaxial cable attenuation and scattering. For further information please consult our brochure “Fully CISPR-Compliant Digital EMC/EMI receivers 10 Hz to 18 GHz”.Antenna Set 30 MHz to 18 GHzProvided by: (800)404-ATECAdvanced Test Equipment Rentals®Ordering Information:AS-05 antenna set 30 MHz to 18 GHz with individual calibration reports.AS-05/TC antenna set 30 MHz to 18 GHz with typical calibration reports.Includes: BC-01 biconical antenna; LP-04 Log-periodic antenna; DR-01Double-rideged antenna; TR-01 wooden tripod; RF cable, 6 GHz, N(m)-N(m), 5 m; Soft carrying case; Rigid carrying case (for DR-01), Operating manual; Calibration reports*.* Individual calibration reports are provided with AS-05.AS-05/TC does not include individual calibration but typical antenna factor.Optional accessories:Additional TR-01 Wooden tripod extensible 60 - 180 cm with antenna mounting adapter for fast horizontal to vertical polaritazion changing. Additional RF cable, 3 GHz, N(m)-N(m), 5 m.Sales Office:Via Leonardo da Vinci, 21/2320090 Segrate (Milano) - ITALY Phone: +39 02 2699871Fax: +39 02 26998700Headquarter:Via Benessea, 29/B17035 Cisano sul Neva (SV) - ITALY Phone: +39 0182 58641Fax: +39 0182 586400E-Mail:**************************Internet: www.narda-sts.itRelated ProductsReceiversAntennasCalibrations service• 7010/00: EMI receiver 150 kHz to 1 GHz • 7010/01: EMI receiver 9 kHz to 1 GHz • 7010/03: EMI receiver 9 kHz to 3 GHz • 9010: EMI receiver 10 Hz to 30 MHz • 9010F: EMI receiver 10 Hz to 30 MHz• 9010/03P: EMI receiver 10 Hz to 300 MHz • 9010/30P: EMI receiver 10 Hz to 3 GHz • 9010/60P: EMI receiver 10 Hz to 6 GHz • 9030: EMI Receiver 30 MHz to 3 GHz • 9060: EMI Receiver 30 MHz to 6 GHz •FR-4003: Field Receiver 9 kHz to 30 MHz• LP-02: Log Periodic Antenna 200 MHz to 3 GHz • LP-03: Log Periodic Antenna 800 MHz to 6 GHz • TR-01: Antenna Tripod• VDH-01: Van der Hoofden test-head 20 kHz to 10 MHz • Antenna Set AS-02 (BC01+LP02+TR01)• Antenna Set AS-03 (BC01+LP02+LP03+TR01) • Antenna Set AS-04 (BC01+LP04+TR01)• RA01: Rod Antenna 9 kHz to 30 MHz• RA01-HV: Rod Antenna 150 kHz to 30 MHz •RA01-MIL: Rod Antenna 9 kHz to 30 MHz• Ansi 63,5 Antenna Factor • SAE ARP 958-D• Free-Space Antenna FactorSPECIFICATIONSFrequency range GainAntenna factor Max input power Connector Dimensions (L x H x W)Weight Colour Impedance ConstructionBC-0130 to 200 MHz -15 +2 dBi typical 8 to 14 dB/m typical 100 W N-female 65 x 65 x 137 cm1,8 kg RAL 703550 Ω nominal AluminiumA S 05-F E N -60801 - S p e c i fi c a t i o n s s u b j e c t t o c h a n g e s w i t h o u t p r i o r n o t i c eAS-05Antenna set 30 MHz to 18 GHzLP-04200 MHz to 6 GHz 6 dBi typical 12 to 40 dB/m typical100 W N-female 78 x 10 x 75 cm 1,1 kg RAL 703550 Ω nominal AluminiumDR-016 to 18 GHz 9 to 16 dBi typical 36 to 41 dB/m typical 150 W N-female 55 x 44 x 177 mm 0,25 kg RAL 703550 Ω nominal AluminiumBC-01 - Antenna Factor 106141822A F (d B /m )3090150210MHz MHz MHz MHz LP-04 - Antenna Factor 155253545A F (d B /m )1356GHzGHz GHz GHz DR-01 - Antenna Factor3634384042A F (d B /m )6101418GHzGHz GHz GHz。
Promega 乐灵特r荧光素 荧光素试剂说明书
Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA1.Description (1)2.Product Components and Storage Conditions (2)3.Principle of the Assay (2)4.Reagent Composition and Testing (2)A.Luciferase/Luciferin (L/L) Reagent (2)B.Reconstitution Buffer (2)5.L/L Reagent Reconstitution (2)6.ATP Assay Procedure (3)A.Luminometer Preparation (3)B.Performing the ATP Assay (3)7.References (5)8.Related Products (5)1.DescriptionThe ENLITEN ®rLuciferase/Luciferin (rL/L) Reagent (a)is intended for the rapid and quantitative detection of adenosine 5´-triphosphate (ATP). The rL/L Reagent is designed to measure 10–11to 10–16mole ATP.Some of the many applications for the ENLITEN ®rL/L Reagent include:•Indirect measurement of bacteria, yeasts, fungi and other microorganisms in foodstuffs, beverages, water, woodpulp, cosmetics and other products.•Assay of enzymes that produce or degrade ATP.•Quantitation of ATP in biological fluids.ENLITEN ®rLuciferase/Luciferin ReagentAll technical literature is available on the Internet at: /tbs/ Please visit the web site to verify that you are using the most current version of this Technical Bulletin. Please contact Promega Technical Services if you have questions on useofthissystem.E-mail:********************.2.Product Components and Storage ConditionsProduct Size Cat.#ENLITEN ®rLuciferase/Luciferin Reagent100 assays FF2021Components include:•1 vial rLuciferase/Luciferin Reagent •12ml Reconstitution BufferStorage Conditions:Prior to reconstitution, the L/L Reagent andReconstitution Buffer must be stored at –20°C.3.Principle of the AssayRecombinant luciferase catalyzes the following reaction (1):ATP + D -Luciferin + O 2→Oxyluciferin + AMP + Pyrophosphate + CO 2+ Light (560nm)When ATP is the limiting component in the luciferase reaction, the intensity of the emitted light is proportional to ATP concentration. Measurement of thelight intensity using a luminometer permits direct quantitation of ATP (2,3).4.Reagent Composition and Testing4.A.rLuciferase/Luciferin (rL/L) ReagentThe rL/L Reagent is supplied lyophilized; once it has been reconstituted withReconstitution Buffer, it contains purified luciferase, D -luciferin, Tris-acetatebuffer (pH 7.75), ethylenediaminetetraacetic acid (EDTA), magnesium acetate,bovine serum albumin (BSA) and dithiothreitol (DTT). Sodium azide (0.02%) isincluded as a preservative.4.B.Reconstitution BufferThe Reconstitution Buffer contains buffer, salts and other components thatmust be added to the vial of lyophilized rL/L Reagent to reconstitute thereagent.5.rL/L Reagent ReconstitutionATP present in fingerprints, glassware, etc. Do not touch the outside of the gloves with your fingers or skin.Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USAbottle labeled Reconstitution Buffer to the vial, recap with the rubber stopper and gently swirl the vial to dissolve the contents. DO NOTshake the dissolved rL/L Reagent. Allow the rL/L Reagent to rehydrate at room temperature for 1hour before using.needed. The activity of the reconstituted rL/L Reagent diminishes roughly 15% after 2 days of storage at 4°C. Be sure to allow the rL/L Reagent to return to room temperature prior to use.If long-term storage is needed, the reconstituted rL/L Reagent can be stored in single-use aliquots at –20°C. Avoid multiple freeze-thaws. The activity of the reconstituted rL/L Reagent diminishes by roughly 50% after two weeks at–20°C.6.ATP Assay Procedure6.A.Luminometer PreparationLight output from the L/L reaction is usually measured in a luminometer.Refer to the instruction manual of your luminometer for proper instrumentsetup and operation. Proper care of your luminometer is important for lowassay “background” and precision in ATP measurements. If your instrument has a reagent delivery system, it is essential that the reagent injectors andsupply tubing are kept clean and aseptic. If the instrument manual does not contain instructions for the cleaning and maintenance of the injectors, contact the manufacturer for instructions. After cleaning the injectors, rinse the system by priming 10 times with sterile, distilled or deionized water. Be sure tocarefully rinse any filters on the reagent tubing as well.6.B.Performing the ATP AssayOnce reconstituted, the rL/L Reagent is sufficient for 100 ATP assays,assuming 0.1ml reagent per assay. Refer to your luminometer instrumentmanual for the recommended procedure for ATP assays. The rLuciferase/Luciferin Reagent has been designed for use in most luminometer protocols.Use disposable pipette tips when adding rL/L Reagent, sample, buffer/water, extractant or ATP standard; use a new tip for each addition. Pipet gently toavoid generating aerosols that could contaminate assay reagents.Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA6.B.Performing the ATP Assay (continued)A “blank” containing rL/L Reagent and the sample buffer and extractant usedto prepare your samples should be run in the assay to determine the amountof “background” relative light units (RLU) to be subtracted from the sampleRLU. Sample RLU values should be corrected for possible buffer/extractantinhibition of light output when converting RLU to ATP mass. This is done byconstructing an ATP standard curve (Figure 1) using an appropriate volume ofbuffer/extractant instead of water to dilute the ATP standard. We recommendthat samples and the ATP standard curve are performed in at least duplicatefor accurate measurement (3). The percent coefficient of variation (standarddeviation divided by the mean RLU times 100) for each set of measurementsshould be 10% or less over the entire assay range.Note:A new ATP standard curve must be made fresh daily or whenever anew aliquot of rL/L Reagent is used.Figure 1. A representative ATP standard curve obtained using the ENLITEN ®rL/L Reagent and a Berthold Luminometer Model 9501.Ten microliters of ATP standard diluted to the proper concentrations was added to a cuvette and assayed using rL/L Reagent according to the luminometer protocol. A 1-second delay time after rL/L Reagent injection and 10-second RLU signal integration time were used.This ATP standard curve is for illustration purposes only ; it is important to Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA7.References1.DeLuca, M.A and McElroy, W.D. (1978) Purification and properties of fireflyluciferase. Methods Enzymol.57, 3–15.2.McElroy, W.D. and DeLuca, M.A. (1983) Firefly and bacterial luminescence: Basicscience and applications. J. Appl. Biochem.5, 197–209.3.Lundin, A. and Thore, A. (1975) Analytical information obtainable by evaluation ofthe time course of firefly bioluminescence in the assay of ATP. Anal. Biochem.66,47–63.8.Related ProductsProduct Size Cat.#GloMax™ 20/20 Luminometer 1 instrument E5311GloMax™ 20/20 Luminometer with Single Auto-Injector 1 instrument E5321GloMax™ 20/20 Luminometer with Dual Auto-Injector 1 instrument E5331GloMax™ 96 Microplate Luminometer 1 each E6501GloMax™ 96 Microplate Luminometer with Single Reagent Injector 1 each E6511GloMax™ 96 Microplate Luminometer with Dual Reagent Injectors 1 eachE6521Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA(a)The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.© 1993, 2001, 2005, 2007, 2009 Promega Corporation. All Rights Reserved.ENLITEN is a registered trademark of Promega Corporation.Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.All prices and specifications are subject to change without prior notice.Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.。
Genebre S.A. Y-type filter 说明书
08908 L’HOSPITALET DE LLOBREGAT Barcelona(Spain)internet: http://www.genebre.esMANUAL DE INSTALACION Y OPERACIÓN DE LOS FILTROS EN “Y” CON CONEXIONES ROSCADAS.Leer toda la información e instrucciones antes de instalar el filtro. El incumplimiento de estas instrucciones puede provocar lesiones corporales o daños a la propiedad.RECEPCIÓN, MANIPULACIÓN E INSPECION1. Inspeccione los daños ocurridos durante el transporte. Informe de daños a la compañía de transporte. Si el filtro no se instala inmediatamente, almacenar el filtro en un lugar limpio y seco.2. Compruebe que la calificación del filtro es mayor o igual a la presión máxima y temperatura de la instalación.3. Inspeccionar la malla y eliminar cualquier material extraño o suelto que pueda ser arrastrado por el fluido.INSTALACIÓNPRECAUCIÓNAntes de la instalación, comprobar la aplicación y la compatibilidad química de los fluidos de proceso con el material de construcción del filtro. Compruebe que la calificación del filtro es mayor o igual a la presión máxima y temperatura de la instalación.PRECAUCIÓNPara las medidas de filtro más grandes, para levantar el filtro poner soportes bajo las conexiones de entrada y de salida.1. En el caso de que exista riesgo de incendio exterior, se debe montar el equipo adecuado para proteger el propio filtro y el resto de la instalación.2. Inspeccionar la malla y eliminar cualquier material extraño o suelto que pueda ser arrastrado por el fluido, antes de instalar el filtro.3. Coloque el filtro en la tubería de forma que el líquido entre en la dirección de la flecha de fundición grabada en el filtro. Siempre instale el filtro con el drenaje en la posición más baja.08908 L’HOSPITALET DE LLOBREGAT Barcelona(Spain)e-mail:******************internet:http://www.genebre.es4. Asegúrese de que se proporciona espacio suficiente para facilitar la apertura de la tapa del filtro y para sacar la malla del filtro.5. Para los filtros de mayor tamaño, los soportes que se coloquen para sostener el filtro deben evitar que las fuerzas y momentos de la tubería actúen sobre el mismo. La tubería debe permitir ajustes debido a las tolerancias del filtro.6. Conectar el filtro a la tubería utilizando las prácticas estándar de instalación en tuberías.7. Una vez instalado el filtro, las conexiones del mismo no deben de poder moverse libremente.PRECAUCIÓNEl filtro en “Y” no está diseñado para ser soporte de anclaje de la línea de tuberías.Asegúrese de apoyar adecuadamente las tuberías a ambos lados del filtro. Tenga cuidado para evitar que las fuerzas y momentos de las tuberías actúen sobre las conexiones del filtro. Pueden producirse daños al filtro si es conectado incorrectamente.8. Los filtros tipo “Y” no tienen rosca de acoplamiento para manómetros. Es necesario colocar un manómetro cerca de la entrada y otro a la salida del filtro para determinar la presión diferencial, para determinar la frecuencia de limpieza del filtro. Estos manómetros son esenciales para la operación segura del filtro y se deben instalar utilizando las prácticas estándar de instalación en tuberías.08908 L’HOSPITALET DE LLOBREGAT Barcelona(Spain)e-mail:******************internet: http://www.genebre.es PUESTA EN MARCHARealice la puesta en marcha de forma gradual. Esto elimina los golpes de ariete al filtro y a otros equipos de la línea.CUANDO LIMPIAR LA MALLAUn aumento de la presión diferencial a través del filtro de 0.3 a 0.7 bar indica que la malla está llena de suciedad y necesita ser limpiada.PRECAUCIÓNPara evitar daños en la malla, NO permitir que la diferencia de presión a través del filtro supere los 1.4 bar.RETIRAR, LIMPIAR O SUSTITUIR LA MALLA. PERIODOS DE PARO DE LA LINEA. PRECAUCIÓNAl abrir el filtro existe peligro debido a la presión interna de dicho filtro. El montado y desmontado del filtro tendrá que llevarse a cabo teniendo la precaución de eliminar lapresión en las conducciones. Una vez aislado el filtro de las conducciones, si el fluido está caliente esperar a que se enfríe antes de abrir el filtro. Siempre quedará líquido en la parte baja del filtro, que saldrá al abrir la tapa de éste. Para proteger al operador debe usar equipo de protección adecuado (gafas, guantes, chalecos, ropa, etc) en consonancia con el fluido de proceso, su presión y su temperatura.1. Poco a poco cerrar las válvulas aguas arriba del filtro. A continuación, abrir lentamente las válvulas tubería abajo del filtro. Después, cerrar también las válvulas tubería abajo del filtro. Asegúrese de que todas las válvulas están bien cerradas.2. El filtro tendrá en su interior presión interna y restos del fluido. Lentamente desenroscar la tapa y retirarla.3. Retirar los desechos de la malla. No permita que los desechos de la malla se sequen, ya que entonces sería difícil quitarlos y limpiar la malla.4. Invertir la malla y lavar los desechos mediante una corriente de aire o de agua en contra del exterior de la malla.5. Inspeccione cada uno de los agujeros de la malla para su limpieza. Reemplazar la malla si es necesario.6. Inspeccionar la junta de sellado de la tapa. Limpiar las superficies de sellado y la tapa.7. Limpiar el alojamiento de la malla. Colocar la malla en ángulo recto en el asiento de la tapa.8. Vuelva a colocar la tapa.9. Siga las instrucciones de puesta en marcha.。
电源保护器数据手册说明书
Electrostatic Discharged Protection Devices (ESD) Data SheetDescriptionUDT26A05L05 is surge rated diode arrays designed to protect high speed data interfaces. It has been specifically designed to protect sensitive components which is connected to data and transmission lines from overvoltage caused by electrostatic discharge (ESD), electrical fast transients (EFT), and lightning.The unique design of the device incorporates one surge rated, and four data lines. Low capacitance steering diodes and a TVS diode in a single package. The low capacitance array configuration allows the user to protect four high speed data or transmission lines.Features● IEC61000-4-2 ESD 15KV Air, 8KV contact compliance ● SOT23-6L surface mount package● Protects four high-speed data lines and one power line ● Array of surge rated, low capacitance diodes ● Working voltage: 5V ● Low leakage current ● Low clamping voltage ● Solid-state silicon avalanche technology ● Lead Free/RoHS compliant● Solder reflow temperature: Pure Tin-Sn, 260~270℃ ● Flammability rating UL 94V-0● Meets MSL level 1, per J-STD-020 ● Marking: B 05B or 054Applications● USB power and data line protection ● 10/100/1000 Ethernet ● Video line protection ● I 2C bus protection● WAN/LAN equipment ● ISDN S/T interface● Microcontroller input protection ● Portable electronicsMaximum RatingsRatingSymbolValue UnitESD voltage (Contact discharge) V ESD ±8kVESD voltage (Air discharge)±15Storage & operating temperature rangeT STG ,T J -55~+150℃Pin ConfigurationElectrical Characteristics (T J=25℃)Parameter Symbol Condition Min. Typ. Max. UnitReverse stand-off voltage V RWM 5V Reverse breakdown voltage V BR I BR=1mA 6 V Reverse leakage current I R V R=5V 5 μAClamping voltage (tp=8/20μs) V C I PP=1A 9.8V Clamping voltage (tp=8/20μs) V C I PP=2A 16VOff state junction capacitance C J 0Vdc,f=1MHzBetween I/Opins and GND2.5 pFApplications InformationESD Protection StandardsIEC61000-4-2Interfaces of consumer electronic equipment are widely specified according to the International Electrotechnical Commission standard IEC61000-4-2. This standard is not targeted towards particular devices but towards general equipment, systems and subsystems that may be involved in electrostatic discharge. consists of a 150pF capacitor and a 330Ω series resistor representing the counterpart to the Device Under Test (DUT).Human Body Model (HBM, MIL-883E method 3015.7)The HBM standard simulates an ESD surge generated by human contact to electronic components.Recommended Soldering ConditionsRecommended ConditionsProfile Feature Pb-Free Assembly Average ramp-up rate (T L to T P) 3℃/second max.Preheat-Temperature Min (T S min) -Temperature Max (T S max) -Time (min to max) (ts)150℃200℃60-180 secondsT S max to T-Ramp-up 3℃/second max.Time maintained above:-Temperature (T-Time (t60-150 seconds Peak Temperature (T P) 260Time within 5 of actual Peak Temperature (t) 20-40 Ramp-down RateTime 25 to Peak TemperatureDimensions (SOT23-6L)Packaging Reel。
ExProfileTM Gene qPCR Array 配套试剂使用说明书
ExProfile TM Gene qPCR Array 配套试剂使用说明书——高通量基因表达量分析阵列配套试剂说明书适用于以下产品All-in-One TM First-Strand cDNA Synthesis Kit for gene qPCR arrayCat.No. AORT-2020 (20 reverse transcription reations)Cat.No. AORT-2060 (60 reverse transcription reations)All-in-One TM qPCR MixCat.No. AOPR-0200 (20 μl × 200 reactions)Cat.No. AOPR-0600 (20 μl × 600 reactions)Cat.No. AOPR-1200 (20 μl × 1200 reactions)Cat.No. AOPR-1000 (20 μl × 1000 reactions)Cat.No. AOPR-4000 (20 μl × 4000 reactions)GeneCopoeia, Inc. 广州易锦生物技术有限公司广州高新技术产业开发区广州科学城掬泉路3 号广州国际企业孵化器F区8 楼邮编：510663电话：4006-020-200邮箱：******************网址：© 2016 GeneCopoeia, Inc.使用说明书ExProfile TM Gene qPCR Array 配套试剂I. 产品介绍II. 试剂盒组成III. 实验前准备IV. 实验步骤V. 数据分析VI. 使用许可与质量保证I. 产品介绍ExProfile TM Gene qPCR Array专为检测不同组织或细胞中特定基因的表达量而设计，这些基因按照预制或定制要求成套设置。
检测结果所显示的差异表达可帮助研究者鉴定或验证这些基因的生物学意义，以及与其研究的重要相关性。
康拉德电子SE产品说明书 - CE-Tinte E-27
Material Safety Data Sheet1. Product & Company Identification:Product Ink jet ink, water based, nonflammableManufacturer:Conrad Electronic SEModel:CE-Tinte E-27Address:Klaus-Conrad-Strasse 1, D-92242 Hirschau Telephone:+ 49 9604408833Date of issue:08.11.20102. Composition /Information on Ingredients:MATERIAL OR INGREDIENT PercentDiethylene glycol5-9%Glycerol4-7%Glycol ether5-8%Isopropanol3-5%Water70-85%Remark: No Ethylene Glycol absolutely3. Hazardous Identification:Inhalation Health risks and symptoms exposure:None reported or known to exist for the product unless listed here.Skin and eye contact health risks and symptoms of exposute:Eye/skin: May cause irritation.Skin: May cause sensitization and/or allergic reaction.Ingestion health risks and symptoms of exposure:May cause gastrointestinal irritation. Vomiting nausea and diarrhea.Acute health hazards:Moderate eye and skin irritation.Chronic health hazards:None known or determined for the product.Carcinogenicity:No data tested.Material Safety Data Sheet4. First Aid Measures:Emergency and first aid proceduresSwallowed: Do NOT induce vomiting. Call a doctor and contact local poison control center immediately. Eye contact: Flush eyes thoroughly with water. Seek medical attention if irritation persists.Skin contact: Wash contaminated areas with soap and water.Inhaled: Removed victim to fresh air.5. Fire Fighting Measures:Flash point: N/A (non-flammable water based)Method used: N/AFlammable limits in air by volume-lower: N/A Upper: N/AExtinguishing media: N/ASpecial firefighting procedures: N/AUnusual fire and explosion hazards: N/A6. Accidental Release Measures:Steps to be taken in case material is released or spilled:Soak up on paper or other absorbent and scoop into cloned containers.Plush area with plenty of water.7. Handling & Store:Precautions to be taken in handling and storingStorage temperature: Standard room temperature (68 °F = 20°C)Special sensitivity: Heat, light, moisturePrecautions: Keep container closed.Other precautions: NoneMaterial Safety Data Sheet8. Exposure Control/Personal Protection:Respiratory protection: Not necessaryVentilation: Mechanical ventilation is acceptable.Protective gloves: RubberEye protection: Goggles or safety glasses with side shields.Other protective clothing or equipment: Impervious cloting where skin contact is unavoidable, eyewash station. Work/hygienic practices: On food, dring, etc. No smoking when handling. Avoid contaminate.9. Physical/Chemical Properties:Boiling point: > 200°FSpecific gravity (H20=1): 1-1.1Physical state: LiquidColor: BK/C/M/Y/PC/PM/R/GInk conductivity: 1.0-5.0PH: 6.0-8.0Specific gravity: 1.0-1.09g/cm³Surface tension: 35-42 dyne/cmInk vosity: 2.5-4.0 centipoisesOdor: MildSolublility in water: C omplete10. Reactivity:Stability: Stable11. Toxicological information:No information available for product unless listed here.12. Ecological information:No information available for product.Material Safety Data Sheet13. Disposal considerations:Waste disposal method:Waste shall be disposed or in accordance with federal, state, and local environmental control regulations.14. Transport Information:D.O.T. Shipping name: Not regulated.15. Regulatory Information:OSHA Status:Product not tested. It is not considered to be toxic per 29CFR1910.1200 but it is considered to be a skin/eye irritant.USA TSCA Status:This product meets the requirements of the Toxic Substances Control Act.International Export/Import status:Not determined for every country. Formulas are proprietary, so ingredient lists may only be released to Customs/Health offices designated to control trade secrets.SARA Title III section 313 toxic chemicals:Product not listed. SARA listed ingredients at or above De Minimis reporting levels are noted in paragraph 2, if any.RCRA hazardous waste number/status:If discarded in its purchased form, this product would not be a federal hazardous waste either by listing or by characteristic. States, however, often have stricter criteria. Users should check with their state regulatory agencies for current hazardous waste criteria. Under RCRA it is the responsibility of the product user to determine at the time of disposal whether a material containing the product or derived from the product should be classified as a hazardous waste (40CFR261.20-24)Ozone-depleting chemicals:No regulated ingredients.New Jersey:Reportable components cas#Water 7732-18-5Dye, nonhazardous supplyprop54Glycol ether FL Prop 184Pyrrolidone, 2-616-45-5Azo dye F/L Prop 121California proposition 65 (carcinogens):Reportable components cas # weight %None unless listed, as follows:California proposition 65 (reproductive toxins):Reportable components cas # weight %None unless listed, as followMaterial Safety Data Sheet16. Other Information:All information appearing herein is based upon data obtained from the manufacturer and/or recognized technical sources. This information is believed to be correct, but does not purport to be all-inclusive and shall be used only as a guide. We make no warranty, express or implied, as to the accuracy or completeness of this information. It is the user´s responsibility to determine the suitability of this information for the adoption of necessary safety precautions and/or compliance with federal, state, and local laws and regulations.·。
TEMALACFD50中文资料
PRODUCT DATA SHEET 02.01.2006REF. NO TCF 00021 (2)Tikkurila Coatings Oy | P.O. Box 53 | Kuninkaalantie 1 | FIN-01301 Vantaa | Tel. +358 (0)9 857 741 | Fax +358 (0)9 8577 6911VAT FI10694878 | Business Identity Code 1069487-8 | Registered OfficeVantaa | TEMALAC FD 50DESCRIPTIONA fast drying, one component semigloss alkyd topcoat, containing anti-corrosive pigments.PRODUCTFEATURES AND RECOMMENDED USES♦ A semigloss topcoat to be used in alkyd systems for steel surfaces. ♦ Excellent gloss and colour retention when exposed to weathering.♦ Ideal for use in machine shops with fast production cycles and in the field. ♦ Suitable also for electrostatic painting.♦ Recommended for steel framework, service platforms and different types ofmachinery and equipment.♦ Due to anti-corrosive pigments can also be used as a single coat system.TECHNICAL DATAVolume solids 47 ± 2 %. (ISO 3233)Weight solids 64 ± 2 %.Specific gravity 1.0 - 1.2 kg / l depending on colourProduct code 181-seriesRecommended film thicknesses and theoretical coverageRecommended film thicknesses Theoretical coveragedry wet40 µm 85 µm 11.8 m 2/l 60 µm130 µm7.7 m 2/lPractical coverage depends on the application method, painting conditions and the shape and roughness of the surface to be coated.Drying timesDFT 40 µm+ 10 ºC + 23 ºC + 35 ºC Dust dry, after 40 min 15 min 10 min Touch dry, after 3 h 1½ h 1 h Recoatable, after12 d 6 d 4 d Recoatable, "wet-on-wet", after1 - 4 h½ - 2 h30 minDrying and recoating times are related to the film thickness, temperature, the relative humidity of the air and ventilation.FinishSemigloss.ColoursRAL, NCS, SSG, BS, MONICOLOR NOVA and SYMPHONY colour cards. TEMASPEED tinting.元器件交易网The above information, based on laboratory tests and practical experience, has been proved valid at the date marked on the product data sheet. When necessary verify the validity of the product data sheet. The quality of the product is ensured by our operational system, based on the requirements of the standards ISO 9001 and ISO 14001. As a manufacturer we cannot be responsible for any damages caused by using the product against our instructions of for inappropriate purposes.TIKKURILA COATINGS OY PRODUCT DATA SHEET 02.01.2006REF. NO TCF 00022 (2)TEMALAC FD 50APPLICATION DETAILSSurface preparationOil, grease, salts and dirt are removed by appropriate means. (ISO 12944-4)Steel surfaces: Blast clean to grade Sa2½. (ISO 8501-1) If blast cleaning is not possible, phosphating is recommended for cold rolled steel to improve adhesion.Primed surfaces: Oil, grease, salt and dirt are removed from the surface by appropriate means. Repair any damage to the primer coat. Note the overcoating time of primer. (ISO 12944-4)Primer TEMAPRIME EE, TEMAPRIME EUR, TEMAPRIME GF, TEMAPRIME ML and FONTECRYL 10.FinishTEMALAC FD 50.Application conditions All surfaces must be dry. The temperature of the ambient air, surface or paint shouldnot fall below + 5 ºC during application or drying. Relative humidity should not exceed 80 %. The surface temperature of the steel should remain at least 3 ºC above the dew point.ApplicationBy airless spray. Mix the paint thoroughly before use. At airless spray application the paint should be thinned 5 - 15 %. Airless spray nozzle tip 0.011" - 0.015" and nozzle pressure 120 - 180 bar. Spray angle shall be chosen according to the shape of the object.ThinnerThinner 1006. Large surfaces Thinner 1053 (slow).Cleaning of equipment Thinner 1006.VOCThe Volatile Organic Compounds amount is 470 g/litre of paint. VOC content in ready to use paint (thinned 15 % by volume) is 522 g/l.HEALTH AND SAFETY Containers are provided with safety labels, which should be observed. Furtherinformation about hazardous influences and protection are detailed in individual health and safety data sheets.A health and safety data sheet is available on request from Tikkurila Coatings Oy.mik220206/tmk020106/181-s元器件交易网。