金黄色葡萄球菌致皮肤脓肿中肥大细胞的活化

Research in Veterinary Science兽医科学研究Activation of mast cells in skin abscess induced by Staphylococcus aureus (S.aureus) infection in mice Zhiqiang Leia,b,c,1, Ding Zhangd,1, Boyong Lue, Wenjiang Zhouf, Decheng Wanga,b,⁎a Medical College of China Three Gorges University, Yichang, 443002, China b The Institute of Infection and Inflammation, China Three Gorges University, Yichang 443002, Hubei, China c Wuxue First People's Hospital, Wuxue 435400, Hubei, Chinad Department of Pathology, Yichang Central People's Hospital, Yichang 443003, Hubei, Chinae The Third Hospital of Yichang City, Yichang 443003, Hubei, Chinaf Shanghai Public Health Clinical Center, Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China 金黄色葡萄球菌致皮肤脓肿中肥大细胞的活化（S）金黄色葡萄球菌感染小鼠支强磊阿，B，C，1，Ding Zhangd，1，薄永璐锷，温江周夫，Decheng Wanga，B，三峡大学仁和医院医学院，中国宜昌443002三峡大学仁和医院感染与炎症研究所，中国湖北宜昌443002武穴市第一人民医院，武穴，湖北，中国，435400宜昌市中心人民医院病理区，中国湖北宜昌443003E宜昌市宜昌第三医院，中国湖北443003复旦大学公共卫生临床中心上海公共卫生临床中心，中国上海201508The skin abscess is a common inflammatory disease that occurs following the ubiquitous S. aureus infection. Inour study, a skin abscess murine model was established and the dynamics of mast cells chemotaxing was evaluated. In the S. aureus-infected mice, severe infiltration of inflammatory cells in the dermis were observed,and mast cells were markedly accumulated in the skins. Besides, tryptase, the marker for mast cells activation,has a positive correlation with mast cell activity. The mast cells identified in the tissues were likely to beactivated since they were associated with cell degranulation and the presence of tryptase. Ourresults suggested,that mast cells and its mediator tryptase contribute to the inflammation of skin abscess induced by S. aureusinfection.皮肤脓肿是普遍存在的金黄色葡萄球菌感染后发生的一种常见的炎症性疾病。

在本研究建立了小鼠皮肤脓肿模型，并对肥大细胞的化学动力学进行了研究。

评价的。

在金黄色葡萄球菌感染的小鼠中，观察到真皮中炎性细胞的严重浸润，肥大细胞在皮肤中明显积累。

此外，胰蛋白酶是肥大细胞活化的标志物，与肥大细胞活性呈正相关。

组织中鉴定的肥大细胞很可能是活化，因为它们与细胞脱颗粒和存在的类胰蛋白酶。

我们的结果表明肥大细胞及其介导的类胰蛋白酶参与金黄色葡萄球菌引起的皮肤脓肿的炎症反应感染。

金黄色葡萄球菌致皮肤脓肿中肥大细胞的活化（S）金黄色葡萄球菌感染小鼠Skin abscesses are fairly common which usually occurin an infection both on the skin surface and within the deeper structures. An abscess may develop and enlarge, depending on whether microorganismsor leukocytes gain the upper hand in any one of a number of locationsin the body (Tinelli et al., 2009). Abscess tends to get worse as time goeson, while it can spread to deeper tissues, and even enters the bloodstream to affect other organs, such as brain, liver, lungs, teeth, andtonsils(Amir et al., 2010). Skin abscess are commonly caused by infectious pathogen, such as the ubiquitous Staphylococcus aureus (S.aureus) bacteria (Chambers, 2001; Lowy, 1998). During the past20 years, an dramatically increasing staphylococcal infections incidence and antibiotic resistance have posed severe burden to humanhealth, in which a notable strain is calledmethicillin-resistant Staphylococcus aureus (MRSA) (Cardo et al., 2002; Grundmann et al., 2006;Lowy, 1998)皮肤脓肿是常见的，通常发生在皮肤表面和深层结构内的感染。

脓肿可能发展和扩大，取决于是否有微生物。

或白细胞在许多位置中的任何一个占优势。

在体内（Tinelli等人，2009）。

脓肿往往随着时间的流逝而变得更坏。

当它可以扩散到更深的组织，甚至进入血流以影响其他器官，如大脑、肝脏、肺、牙齿，以及扁桃体（阿米尔等，2010）。

皮肤脓肿通常是由感染性病原体引起的，如普遍存在的金黄色葡萄球菌（S）。

金黄色葡萄球菌（室，2001；洛伊，1998）。

在过去20年来，葡萄球菌感染的发病率和耐药性急剧增加，给人类带来了沉重的负担。

健康方面，其中显著菌株被称为耐甲氧西林金黄色葡萄球菌(MRSA)(Cardo等人，2002；Grundmann等人，2006)；洛伊，1998）Mast cells which are main effector cells of inflammation, resident intissues throughout the body and is abundant in the boundaries betweenthe outside and internal world, such as the skin, mucosa of the respiratory and digestive tract (Galli et al., 2005; Metcalfe et al., 1997).Mast cells play a pivotal role in the inflammatory process. Underactivation, they rapidly release its typical granules and various hormonal mediators into the interstitium, such as tryptase, histamine and5-HT to exert multiple actions in the tissue microenvironments (Galliet al., 1999; Marshall, 2004; Wang et al., 2008). It demonstrated thatmast cells can be activated after infection with RSV or cytomegalovirusin bovine and rodent models (Gibbons et al., 1990; van Schaik et al.,1999). Others have demonstrated that mast cells perform a positive rolein viral invasion by dodging immunosurveillance and aggravating theinflammatory responses (King et al., 2000; Wang et al., 2008). Thesefindings strongly suggest the importance of mast cells in the interplaybetween pathogens and their contribution in the induction of acuteinflammation response 肥大细胞是炎症的主要效应细胞，驻留在组织遍及全身，并有丰富的界限。