Rabbit Anti-Antithrombin III抗体说明书
兔抗人胸腺细胞免疫球蛋白说明书
【适应症】 移植用免疫抑制剂：预防和治疗器官排异反应。 预防造血干细胞移植术后的急性和慢性移植物抗宿主病（GvHD）。 治疗激素耐受的移植物抗宿主病 (GvHD)。 血液学疾病：治疗再生障碍性贫血。
【规格】 25mg/瓶 （每瓶含兔抗人胸腺细胞免疫球蛋白25mg，复溶后体积5ml。）
• 再生障碍性贫血： 2.5~3.5mg/kg/日，连续5日。相应的累积剂量12.5~17.5mg/kg。 此产品用于治疗再障的临床对照试验暂缺。
剂量调整 对于包括血小板减少症和/或白细胞减少症（特别是淋巴细胞减少症和中性粒细胞减少 症）的血液学影响，通过调整药物剂量，可逆转上述不良反应。当血小板减少和/或白细 胞减少不是由基础疾病所致或者与使用兔抗人胸腺细胞免疫球蛋白相关时，建议采用下 列剂量调整（见【用法用量】）： 如果血小板计数在50,000~75,000/mm3或者白细胞计数2,000~3,000/ mm3必须考虑减少用
颗粒物，轻轻旋转瓶，直至颗粒消失。如果颗粒物持续存在，则丢弃。 推荐配制后即刻使用。每瓶药物只可以单次使用。根据每日剂量配制相应瓶数的兔抗
人胸腺细胞免疫球蛋白。根据需要，选择合适的瓶数配制。 为了避免不慎输入混有颗粒的溶液，推荐在输注时采用0.2μm过滤器进行在线过滤。 用等渗稀释液（0.9%生理盐水或5%葡萄糖溶液）稀释每日剂量药物至50~500ml（通常
比例50ml/瓶）。 配制的溶液应该处理。
2
【不良反应】
应用以下CIOMS发生率，当适用时： 非常常见≥10%；常见≥1%和<10%；不常见≥0.1%和<1%；罕见≥0.01%和<0.1%；非 常罕见<0.01%；未知（不能从现有数据中估计）。
感染和侵染 感染（包括感染再激活） 败血症
兔抗人胸腺细胞免疫球蛋白
兔抗人胸腺细胞免疫球蛋白【药品名称】通用名称：兔抗人胸腺细胞免疫球蛋白英文名称：Rabbit Anti-human Thymocyte Immunoglobulin【成份】本品主要成分：兔抗人胸腺细胞球蛋白每小瓶5ml含25mg兔抗人胸腺细胞球蛋白辅料：甘氨酸、氯化钠、甘露醇。
注射用水。
【适应症】1.移植用免疫抑制剂：预防和治疗器官排异反应。
治疗激素耐受和移植物抗宿主病（GvHD）。
2.血液学疾病：治疗再生障碍性贫血。
【用法用量】用量根据不同的适应症选择剂量，建议参考剂量为：器官移植的免疫抑制治疗：预防器官排异：肾脏、胰腺、肝脏移植后1.25-2.5毫克/kg/日，1至3周，心脏移植后3-10日。
终止用药时，无需逐渐减量。
治疗器官排异：2.5-5毫克/kg/日，至临床症状消失和生物学指标改善。
再生障碍性贫血：2.5-5毫克/kg日，连续5日。
此产品用于治疗再障的临床实验暂缺。
激素耐受和急性移植物抗宿主病治疗：剂量【不良反应】滴注兔抗人胸腺细胞免疫球蛋白时和用后有如下副反应的报道：全身性副反应为寒战、发热、心跳过速、呕吐和呼吸困难。
局部副反应有输液处局部疼痛及末梢血栓性静脉炎的报道。
罕见迟发性过敏反应，如初次使用后7-15日，可能会发生血清病（发热、搔痒、皮疹伴有关节痛）。
速发严重过敏反应极为罕见。
常见和极严重的副反应发生在第一次滴注后。
有些副反应的发生机理是与细胞分裂释放有关。
应用皮质醇和抗组胺制剂进行预防治疗并减慢滴速或增加稀释液量（等渗0.9%生理盐水或5%葡萄糖溶液）可降低或减轻【禁忌】急性感染时，禁用免疫抑制治疗。
对兔蛋白或本品其他成分过敏者。
【注意事项】兔抗人胸腺细胞免疫球蛋白必须住院并在严密监控状态下使用。
有些严重副反应可能与滴速有关。
应严格执行使用方法中提示的滴速要求。
输药期间必须自始至终严密监控患者。
由于可能发生血清病，应向接受兔抗人胸腺细胞免疫球蛋白治疗者说明。
如果发生副反应，减慢滴速或中断滴注至症状缓解。
免疫兔制备抗体流程
免疫兔制备抗体流程英文回答：Preparation of Antibodies in Immunized Rabbits.The process of producing antibodies in immunizedrabbits involves several key steps:1. Immunization: The rabbit is injected with the antigen of interest, which triggers an immune response. The antigen can be a protein, peptide, or other substance that the body recognizes as foreign.2. Antibody Production: In response to the antigen, the rabbit's immune system produces antibodies that arespecific to the antigen. These antibodies are produced by B cells, which are a type of white blood cell.3. Collection of Serum: Once the rabbit has produced a sufficient level of antibodies, blood is collected from therabbit. The serum, which contains the antibodies, is then separated from the blood cells.4. Purification of Antibodies: The serum is thenpurified to isolate the antibodies of interest. This can be done using various techniques, such as affinity chromatography or protein A/G purification.5. Characterization of Antibodies: The purified antibodies are characterized to determine their specificity, affinity, and other properties. This is important for ensuring that the antibodies are suitable for theirintended use.中文回答：免疫兔制备抗体流程。
FLAG M2单抗产品说明书
Product InformationMonoclonal ANTI-FLAG® M2, Clone M2 Produced in Mouse, Affinity Isolated AntibodyF1804Storage Temperature −20 °CProduct DescriptionMonoclonal ANTI-FLAG® M2 is a mouse-derived, affinity-purified IgG1 monoclonal antibody that binds to fusion proteins containing a FLAG® peptide sequence.1 The M2 antibody will recognize a FLAG® peptide sequence at the N-terminus,Met-N-terminus, C-terminus, or internal sites of a fusion protein. Binding of the M2 antibody is not dependent on calcium.Monoclonal ANTI-FLAG® M2 is useful for detection, identification, and capture of fusion proteins that contain a FLAG® peptide sequence by common immunological procedures, such as Western blotting, immunofluorescence, and immunoprecipitation. ReagentSupplied at a concentration of ∼ 1 mg of protein per mL of solution, and formulated in 50% glycerol,10 mM sodium phosphate, and 150 mM NaCl,pH 7.4. The formulation contains no antimicrobial preservatives.Antigenic binding siteN-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C SpecificityThe monoclonal antibody detects only the target protein band(s) on a Western blot from an E. coli, plant or mammalian crude cell lysate.SensitivityThe monoclonal antibody detects as little as 2 ng of target protein by dot blot. The Western blot is tested down to 10 ng, but may detect lower using the procedure detailed below. Precautions and DisclaimerFor R&D use only. Not for drug, household,or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.Preparation InstructionsImmediately prior to use, dilute the monoclonal antibody in Tris-buffered saline (TBS), pH 8.0, with 3% nonfat milk (Cat. No. T8793). Dilutions in the described procedures are provided as guidelines. Adjust the antibody concentration to maximize detection sensitivity and minimize background. StorageStore the undiluted antibody at −20 °C in working aliquots. The product, as formulated, will not freeze when stored at the recommended temperature. Note: Over time, small amounts of purified antibodies can precipitate due to intermolecular hydrophobic interactions. If precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in most applications.ProceduresWestern Blot Immunostaining2-5Note: this procedure is based on chemiluminescent detection using Chemiluminescent Peroxidase Substrate-1 (Cat. No. CPS160). Dilutions of both primary and secondary antibodies may require optimization when using other substratesor conditions.1.Separate fusion proteins containing aFLAG® peptide sequence from sample lysatesusing a standard SDS-PAGE protocol. Load2.5–10 µg of total protein lysate per lane.2.Transfer proteins from the gel to a nitrocellulosemembrane, Immobilon®-P, or otherpolyvinylidene difluoride (PVDF) membrane. ThePVDF membrane may provide greaterdownstream sensitivity.3.Wash the blot in at least 0.5 mL/cm2 of purifiedwater for 2–3 minutes employing gentle agitation (50–60 rpm).4.Block the blot with at least 0.5 mL/cm2 ofTris Buffered Saline (TBS), pH 8.0, with 3%nonfat milk for 30 minutes at room temperature, employing gentle agitation.5.Remove the blocking agent and wash once with0.5 mL/cm2 of Tris buffered saline, pH 8.0(Cat. No. T6664).6.Add the desired concentration of monoclonalantibody to the blot. A final antibodyconcentration of 1 µg/mL (1:1,000 dilution of the antibody as supplied) in at least 0.5 mL/cm2 ofTBS with 3% nonfat milk is suggested. Incubateat room temperature for 30 minutes employinggentle agitation.Note: Dilutions must be optimized for differentsubstrates and systems.7.Decant off the Monoclonal ANTI-FLAG® M2solution and wash once with at least 0.5 mL/cm2of TBS, pH 8.0.8.Add the secondary antibody in the form ofAnti-Mouse IgG-Peroxidase (Cat. No. A9044) orequivalent. The concentration of secondaryantibody must be optimized based on thesubstrate being used. For detection usingChemiluminescent Peroxidase Substrate-1, a final secondary antibody dilution of 1:30,000 should be employed. Specifically, it is suggested theantibody as supplied be diluted in at least0.5 mL/cm2 of TBS with 3% nonfat milk. Incubatethe blot employing gentle agitation at roomtemperature for 30 minutes. 9.Wash the blot at least three times for a total of15 minutes (5 minutes per wash) in TBS with0.05% TWEEN® 20, pH 8.0 (Cat. No. T9039).Agitate gently, employing at least 0.5 mL/cm2 ofwash solution.10.Develop the blot using ChemiluminescentPeroxidase Substrate-1 or an equivalent reagentfor 5 minutes. Do not agitate the blot during this incubation step. Drain briefly and wrap inplastic film.11.Expose BioMax® Light film to the blot for a rangeof times from several seconds up to 10 minutes.It is recommended that a quick exposure of10–30 seconds be performed to determine theoptimal exposure time needed. If the signal is too intense even at the short exposure times, allowthe signal to decay over a 1–8 hour period(or longer if necessary), and then re-exposethe film.Indirect ImmunofluorescentCytochemical StainingMonoclonal ANTI-FLAG® M2 may be utilized in immunocytochemical staining procedures when used in conjunction with a labeled secondary antibody (indirect).6 A generic procedure for adherent cell staining is described, using immunofluorescence, employing an Anti-Mouse IgG-FITC conjugate asthe label.1.Grow and transfect cells on coverslips.2.Fix the cells by incubation with phosphatebuffered saline, pH 7.4 (Cat. No. P3813),containing 4% paraformaldehyde (Cat. No.P6148), and 4% sucrose (Cat. No. S1888) for15 minutes at room temperature.3.Wash the fixed cells with PBS for 5 minutes.Repeat once.4.Permeabilize the cells by incubation with 0.25%TRITON™ X-100 (Cat. No. T9284) in PBS for5 minutes.5.Wash the cells with PBS for 5 minutes.Repeat once.6.Block by incubation with 10% bovine serumalbumin (Cat. No. A9647) in PBS (10% BSA/PBS) for 30 minutes at 37 °C.7.Incubate with Monoclonal ANTI-FLAG® M2 dilutedin the range of 1:500 to 1:2,000 in 3% BSA/PBSfor 2 hours at 37 °C.8.Wash with PBS for 5 minutes. Repeat twice.9.Incubate with the secondary antibody, Anti-MouseIgG- FITC (Cat. No. F9137) at a 1:1,000 dilutionin 3% BSA/PBS for 45 minutes at 37 °C.10.Wash with PBS for 5 minutes. Repeat twice.11.Mount coverslips with cells side down on glassslides using a small drop of mounting mediumsuch as polyvinyl alcohol for semi-permanentmounting. The inclusion of an anti-fading agentlike DABCO® in the mounting medium(25–100 mg/mL, for example Cat. No. 10981) isstrongly recommended. Seal coverslips to glassslides (with nail polish, for instance).12.Examine by fluorescence microscopy. FITC has anabsorption maximum at 492 nm with an emission maximum at 520 nm. Immunoprecipitation (IP)Monoclonal ANTI-FLAG® M2 may be used in IP procedures when used in conjunction with an insoluble carrier matrix, such as a Protein G resin. Alternatively, EZview™ Red Protein G Affinity Gel (Cat. No. E3403) or the Protein G Immunoprecipitation Kit (Cat. No. IP50) may be used. EZview Red ANTI-FLAG® M2 Affinity Gel(Cat. No. F2426) or ANTI-FLAG® M2 Affinity Gel (Cat. No. A2220) may be utilized directly for IP. See reference 5 for general protocols.Enzyme Immunoassay (EIA)Monoclonal ANTI-FLAG® M2 may be used in EIA procedures. Typically, a fusion protein containing a FLAG® peptide sequence is directly adsorbed (or otherwise presented) within the wells of a multiwell polystyrene plate. The Monoclonal ANTI-FLAG® M2 antibody may be diluted up to 1:50,000 for subsequent incubation within the plate wells. Detection may be accomplished using Anti-Mouse IgG-Peroxidase (Cat. No. A9044) or equivalent, diluted 1:10,000, followed by an appropriate substrate for visualization.We also offer the ANTI-FLAG® High Sensitivity, M2 coated 96-well plate (Cat. No. P2983) for EIA-based screening applications. References1.Brizzard, B.L., et al., Immunoaffinity purificationof FLAG® epitope-tagged bacterial alkalinephosphatase using a novel monoclonal antibodyand peptide elution. BioTechniques, 16(4):730-735 (1994).2.Bjerrum, O.J., and Heegaard. N.H.H., CRCHandbook of Immunoblotting of Proteins, Volume I, Technical Descriptions. CRC Press(Boca Raton, FL), pp. 229-236 (1988).3.Dunbar, B.S. (ed.), Protein Blotting: A PracticalApproach. IRL Press (New York, NY),pp. 67-70 (1994).4.Fortin, A., et al., A 56- to 54-kilodalton non gratasignal in immunoblot analysis using thehorseradish peroxidase chemiluminescencesystem. Biochem. Cell Biol., 72(5-6):239-243 (1994).5.Harlow, E., and Lane, D., Antibodies: ALaboratory Manual. Cold Spring Harbor Laboratory Press (Cold Spring Harbor, NY) (1988).6.Ciaccia, A.V., and Price, E.M., IBI FLAG Epitope,1: 4-5 (1992)..Troubleshooting Guide (Western Blot Immunostaining Procedure) Problem Possible Cause SolutionFusion protein is not detected. Protein is notexpressed.Verify nucleic acid sequence and reading frame of the FLAG® fusionprotein in vector construct. If sequence is present, attempt tooptimize expression.Target proteinpoorly representedin sample.Positive controls (10 ng/lane recommended) should always beincluded. If the positive control works, the sample may not containthe FLAG® fusion protein of interest, or it may be present atconcentrations too low to detect. Immuno-precipitation withANTI-FLAG® M2 Affinity Gel (Cat. No. A2220) may be required forlow concentrations of FLAG fusion proteins.Positive controls we have available:•Amino-terminal FLAG-BAP™ Fusion Protein (Cat. No. P7582).•Carboxy-terminal FLAG-BAP™ Fusion Protein (Cat. No. P7457).•Amino-terminal Met-FLAG-BAP™ Fusion Protein (Cat. No. P5975).Defective detectionreagentsRun appropriate controls to ensure performance. Use 10 ng/lane of acontrol FLAG-BAP™-fusion protein as a positive control. If no signal isobtained with the control, repeat the procedure using a fresh lot ofsecondary antibody-HRP conjugate, along with freshly preparedreagents.Inadequateexposure timeusingchemiluminescentsystem.If no signal is observed on the film, expose for longer times. It isrecommended to try exposure times ranging from about 5 seconds toas long as 10 minutes.Inappropriate film Switch to film designated for chemiluminescent detection such asBioMax® Light.No target proteinpresent onmembrane.Verify transfer onto the membrane by visualizing proteins usingPonceau S solution (Cat. No. P7170). If possible, a positive controlshould always be run to ensure that the detection systemcomponents are functioning normally. Pre-stained protein markers(for example, Cat. No. C1992 or C4861) may also be used to verifycomplete transfer of proteins from gel to membrane.Antigen is coveredby blockingreagent, due tooverblocking.Masking of a signal can occur if the blocking reagent (such as caseinor gelatin containing blocking buffers) is used at an excessively highconcentration. A dilution ranging from 1:1 to 1:3 may be performedto decrease the concentration of blocking reagent. If the problempersists, use TBS with 3% nonfat milk (Cat. No. T8793).Antibodyconcentration isnot optimal.Determine the optimal working dilution for the MonoclonalANTI-FLAG® M2 antibody via titration. Consider using a higherconcentration of antibody, if no signal or a weak signal is detected.Also, antibody used at an excessively high concentration can causesignal inhibition, especially in chemiluminescent detection systems.The life science business of Merck operatesas MilliporeSigma in the U.S. and Canada.Merck and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.Problem Possible Cause SolutionHigh non-specific background Cellular extract concentration istoo high.2.5–10 µg of total lysate protein perlane is usually enough to obtain agood signal. Load less cellularextract or serially dilute the cellularextract to determine the optimalsignal to noise ratio.Monoclonal ANTI-FLAG® M2antibody concentration is too high.Dilute the Monoclonal ANTI-FLAG®M2 antibody to a concentrationranging from 0.1–0.5 µg/mL. UseTBS with 3% nonfat milk asthe diluent.Secondary antibody cross-reactivity.For the secondary antibody, it isrecommended that users initiallyemploy dilutions of 1:30,000.Higher dilutions may be necessary,or a more specific secondaryantibody should be used.Monoclonal ANTI-FLAG® M2antibody cross-reacts with naturallyoccurring FLAG®-like epitopes.Increasing the temperature to37 °C during the blocking, binding,and wash steps may reducecross-reactivity. Lysates frommock-transfected controls(transfected with plasmid lackinginsert DNA) will help distinguish theFLAG®-fusion proteins from othercross-reacting proteins.NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.The information in this document is subject to change without notice and should not be construed as a commitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page at /techservice.Standard WarrantyThe applicable warranty for the products listed in this publication may be found at /terms. Contact InformationFor the location of the office nearest you, go to /offices.。
兔子Ⅲ型前胶原肽PⅢNPELISA试剂盒使用说明书
兔子Ⅲ型前胶原肽(PⅢNP)ELISA试剂盒使用说明书Elisa kit规格：48孔配置/96孔配置标准品稀释液：1.5ml×1瓶酶标试剂：3 ml×1瓶（48）/6 ml×1瓶（96）【兔子Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒elisa试剂盒】本试剂仅供研究使用计算：以标准物的浓度为横坐标，OD值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。
试剂盒组成：封板膜:2片（48）/2片（96）说明书:1份密封袋:1个标准品：2700ng/L 0.5ml×1瓶0.5ml×1瓶2-8℃保存酶标包被板: 1×48 1×96 2-8℃保存样品稀释液: 3ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液: 3ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液: 3ml×1瓶 6 ml×1瓶2-8℃保存终止液: 3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液:（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存实验原理：本试剂盒应用双抗体夹心法测定标本中兔子Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒水平。
用纯化的兔子Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒，再与HRP标记的Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA 试剂盒抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
碧云天生物技术 HLA-DR Rabbit Monoclonal Antibody 说明书
碧云天生物技术/Beyotime Biotechnology 订货热线：400-168-3301或800-8283301 订货e-mail ：****************** 技术咨询：***************** 网址：碧云天网站 微信公众号HLA-DR Rabbit Monoclonal Antibody产品编号 产品名称包装 AF2065HLA-DR Rabbit Monoclonal Antibody50µl产品简介：来源 用途交叉反应性 分子量 RabbitWB, IF, IHC, ICC, FCH, M, R29 kDaWB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.配套提供了Western 一抗稀释液，可以用于Western 检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：抗体详细信息如下:About this AntibodyName HLA-DR Rabbit Monoclonal AntibodyCategory Rabbit Monoclonal Antibody (RabMAb); Primary antibody Isotype IgGPurification Affinity purification About the ImmunogenImmunogen Recombinant protein Gene ID 3122(Human) SwissProt P01903(Human) Synonyms HLA-DRA1; MLRWCategoryImmunology/InflamationBackgroundMajor histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II α/β dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.包装清单：产品编号 产品名称包装 AF2065 HLA-DR Rabbit Monoclonal Antibody50µl AZ050 Western 一抗稀释液50ml —说明书1份保存条件：WBIP IFIHC ICCFCELISA ChIP 1:1,000-1:5,000 -1:50-1:2001:50-1:2001:50-1:2001:50-1:100--2 /3 AF2065 HLA-DR Rabbit Monoclonal Antibody400-1683301/800-8283301 碧云天/BeyotimeHLA-DR Rabbit Monoclonal Antibody -20ºC 保存，Western 一抗稀释液-20ºC 或4ºC 保存，一年有效。
Immunoway免疫组织化学试剂 Polymer HRP Goat Anti Rabbit I
Polymer HRP * Goat Anti Rabbit IgG(H+L)【产品货号】RS0010【产品名称】1、通用名称：Polymer HRP羊抗兔二抗即用型（免疫组织化学）2、英文名称：Polymer HRP * Goat Anti Rabbit IgG(H+L)【包装规格】类别规格工作液10mL/瓶、100mL/瓶【预期用途】免疫组织化学染色，配合Immunoway常规兔来源的浓缩型或即用型一抗使用。
【作用原理】数个过氧化物酶分子和数个二抗分子结合在同一多聚体上构成了一种简单而灵敏的显色系统【主要组成成份】磷酸盐缓冲液0.01mol/LGoat anti Rabbit HRP 大于0.05mg/L【储存条件及有效期】1、储存要求：2℃～8℃密封保存。
2、有效期：一年。
【样本要求】新鲜活检或外科样本组织，甲醛固定，固定时间8-24小时要求取材、脱水、石蜡包埋并制成蜡块。
配合Immunoway常规浓缩型或即用型一抗使用。
【检验方法】1、人体组织样本先经甲醛固定。
2、固定后的组织经过脱水等一系列操作制备成蜡块。
3、用切片机把组织蜡块切成切片。
4、手工操作免疫组化实验。
常规推荐步骤如下：4.1. 将需要进行实验的组织切片取出，插入玻片架上后放入60-65℃的烤箱中，烤片1-1.5h。
4.2. 将切片置于以下染色缸中梯度反应：二甲苯10分钟×2次二甲苯5分钟无水乙醇5分钟×2次95%乙醇5分钟85%乙醇5分钟PBS清洗3分钟×3次4.3. 抗原修复（方法见一抗供应商，或采用常规高压、微波、酶修复等）4.4.根据组织大小直接滴加内源性过氧化物酶阻断剂到组织上，通常为100uL, 直至完全覆盖组织。
室温10分钟，PBS清洗3分钟×3次。
此步骤通常在水浴法、酶法等温和抗原修复后加入。
或者组织本身含有较多内源性过氧化物酶中加入。
4.5. 根据组织大小滴加一抗到组织上，通常为100uL,直至完全覆盖组织。
抗人T细胞兔免疫球蛋白
抗人T细胞兔免疫球蛋白Kang Ren T Xibao Tu MianyiqiudanbaiAnti-human T Lymphocyte Rabbit Immunoglobulin本品系由人T淋巴细胞免疫家兔后,取其血清或血浆经去除杂抗体、纯化、浓缩后,再经病毒灭活处理并加入适宜稳定剂后冻干制成。
不含防腐剂和抗生素。
1 基本要求生产和检定用设施、原料及辅料、水、器具、动物等应符合“凡例”的有关要求。
2 制造2.1 免疫血清2.1.1 免疫抗原免疫用抗原为人胸腺细胞,或符合“血液制品生产用人血浆”中供血浆者标准的健康人血液分离的人淋巴细胞。
胸腺供体为HBsAg、HCV抗体、HIV-1/HIV-2抗体和梅毒血清学检查应为阴性。
分离后T淋巴细胞数应不低于总细胞数的90%,红细胞数应不高于总细胞数的5%。
2.1.2 免疫动物免疫用家兔至少应符合普通级实验动物的要求(附录ⅩⅢ B和附录ⅩⅢ C),体重为2000~2500克,检疫合格者方可使用。
2.1.3 免疫方法按批准的免疫程序免疫。
2.1.4 采血及分离血清加强免疫后,淋巴细胞毒试验效价达1∶400时即可采血。
分离的血清置-20℃以下保存。
保存期不应超过2年。
2.2 原液2.2.1 混合血清经56℃水浴30分钟灭能,辛酸-硫酸铵盐析分离纯化或经批准的其他分离纯化法,杂抗体吸收,再用DEAE-sephadex A-50色谱纯化抗人T细胞兔免疫球蛋白。
杂抗体吸收用的人红细胞、人血小板、人胎盘组织及人血浆的供给者应符合“血液制品生产用人血浆”中供血浆者标准。
2.2.2 原液检定按3.1项进行。
2.3 半成品制备2.3.1 配制加入适量麦芽糖或其他适宜稳定剂。
按成品规格以灭菌注射用水稀释至所需蛋白质浓度,并适当调整pH值及钠离子浓度。
2.3.2 半成品检定按3.2项进行。
2.4 成品制备2.4.1 分批应符合“生物制品分批规程”规定。
2.4.2 分装及冻干应符合“生物制品分装和冻干规程”规定。
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Rabbit Anti-Antithrombin III/SERPINC1
Cat.Number:DM-1636R
Quantity size:0.1ml
Concentration:1mg/ml Buffer=0.01M TBS(pH7.4)with1%BSA,0.03%Proclin300and50% Glycerol.
Background:The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily.This protein inhibits thrombin as well as other activated serine proteases of the coagulation system,and it regulates the blood coagulation cascade.The protein includes two functional domains:the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus.The inhibitory activity is enhanced by the presence of heparin.More than120mutations have been identified for this gene,many of which are known to cause antithrombin-III deficiency.[provided by RefSeq,Jul2009].
Also known as:AntithrombinIII;Antithrombin III;AT3;AT III;AT3;ATIII;MGC22579;Serine(or cysteine)proteinase inhibitor clade C(antithrombin)member1;Serine Proteinase Inhibitor Clade C Member1;SERPIN C1;Serpin peptidase inhibitor clade C(antithrombin)member1;SERPINC1; ATH III.
Specificity:
●Rabbit Polyclonal IgG,affinity purified by Protein A.
●Reacts with:Human,Mouse,Rat,Pig,Cow,Sheep,.
●Immunogen:KLH conjugated synthetic peptide derived from human Antithrombin III.
●Predicted Molecular Weight:51kDa.
Storage:Shipped at4℃,Store at-20℃(Avoid repeated freeze/thaw cycles). Application:WB=1:100-500ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500 IF=1:100-500
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Important Note:This product as supplied is intended for research use only,not for use in human, therapeutic or diagnostic applications.。