微生物名词解释
微生物学名词解释

微生物学名词解释1.微生物:不是分类学上的名词,而是指肉眼难以看清,需要借助光学显微镜或电子显微镜才能观察到的一切微小生物(<0.1mm)的总称。
2.模式生物:背景清晰,基因组小,易于测量和分析,可以从中获得经验,改进技术方法。
例如大肠杆菌和酵母、线虫、果蝇和小鼠。
3.酵母菌:一般泛指能发酵糖类的各种单细胞真菌。
4.霉菌:它是丝状真菌的通用名称。
通常指菌丝体较发达且不产生大的肉质子固体结构的真菌。
5.病毒:由一个或数个rna或dna分子构成的感染性因子,通常(但并非必须)覆盖有由一种或数种蛋白质构成的外壳,有的外壳外还有更为复杂的膜结构;这些因子能将其核酸从一个宿主细胞传递给另一个宿主细胞;它们能利用宿主的酶系统进行细胞内的复制;有些病毒还能将其基因组整合入宿主细胞dna,依靠这种机制,或是导致持续性感染发生,或是导致细胞转化,肿瘤形成。
6.病毒颗粒(Virus particle):病毒的细胞外颗粒形式和病毒的传染性形式。
它是一组可以独立复制的遗传物质(DNA或RNA)。
它们被自己编码的蛋白质外壳所包围。
有些人还有一个胶囊,保护他们的遗传物质免受环境损害,并作为载体将遗传物质从一个宿主细胞转移到另一个宿主细胞。
7.衣壳粒:病毒的最小形态单位,由1-6个多肽分子折叠缠绕成的蛋白质亚单位(三级结构)。
8.衣壳(壳):由衣壳颗粒以对称形式规则排列而成的病毒壳。
它含有核酸,形状各异。
9.核衣壳(核壳):病毒蛋白质壳体与病毒核酸的合称,为病毒的基本结构。
10.暴露毒物颗粒:只有核壳基本结构的毒物颗粒称为暴露毒物颗粒。
如烟草花叶病毒和脊髓灰质炎病毒的病毒颗粒。
11.包膜毒粒:有些病毒如流感病毒,在核壳外还具有包膜,有的包膜上还有刺突,这类毒粒称为包膜毒粒。
包膜的结构具有高度稳定性。
12.强效噬菌体:在感染宿主细胞后,它可以在细胞内正常复制,最终杀死细胞,形成一个裂解周期。
13.轻度(溶原性)噬菌体:感染宿主细胞后,复制周期无法完成。
微生物名词解释

医学微生物学名词解释1.微生物:微生物是指自然界存在的一大群种类繁多的微小生物,它们结构简单、体积微小,不能用肉眼直接观察到,必须借助光学或电子显微镜放大数百倍或数千倍以上才能观察到的微小生物。
2.病原微生物:对人类和动物、植物具有致病性的微生物称病原微生物。
3.非细胞型微生物:没有细胞结构,由核酸和蛋白质组成,只能在活细胞内生长繁殖的最小的一类微生物。
4.原核细胞型微生物:仅有原始核质,无核膜和核仁,细胞器不发达的微生物。
5.真核细胞型微生物:细胞核分化程度高,有核膜和核仁,细胞器完整的微生物。
6.正常菌群:是指正常人体体表及与外界相通的腔道中存在着多种微生物,正常情况下它们与宿主间以及它们之间保持相对平衡,通常对人体有益无害,称为正常菌群。
7.菌群失调:是指在原微生境或其他有菌微生境内正常微生物群发生的定量和定性的异常变化。
这种变化主要是量的变化,故也称比例失调。
8.条件致病菌:某些微生物在正常情况下不致病,但在正常菌群当其菌群失调、定位转移、宿主转换或宿主抵抗力的严重降低时,可引起疾病,称条件致病菌。
9.细菌是—类具有细胞壁的单细胞原核微生物。
它们形体微小,以微米(μm)为测量单位,结构简单,无成形的细胞核,无核膜和核仁,除核蛋白外无其他细胞器。
10.L 型细菌细菌细胞肽聚糖受到破坏或肽聚糖的合成被抑制后,在高渗条件下,有部分细菌仍能存活而变成细胞壁缺陷细菌,称为L 型细菌。
11.质粒是细菌染色体外的遗传物质,存在于细胞质中,为闭合的环状双股DNA,带有遗传信息,控制细菌的某些特定的遗传性状。
12.荚膜某些细菌如肺炎球菌、炭疽杆菌等在细胞外面有一层较厚的粘液性物质,称为荚膜。
13.鞭毛有些杆菌、弧菌及螺形菌的菌体上具附有细长、弯曲的丝状物,称为鞭毛。
它是细菌的运动器官。
14.菌毛有些细菌表面在电镜下可见有较鞭毛短而细的丝状物,称为菌毛。
菌毛包括性菌毛和普通菌毛两种。
15.芽胞某些菌在一定的环境条件下,细胞质脱水、浓缩,在菌体内形成折光性强、不易着色的圆形或卵圆形的小体,称为芽胞。
微生物学名词解释

微生物名词解释1.微生物:是一切肉眼看不见或看不清的微小生物的总称。
2.微生物学:是在分子、细胞或群体水平上研究各类微小生物的形态结构、生长繁殖、生理代谢、遗传变异、生态分布和分类进化等生命活动的基本规律,并将其应用于工业发酵、医学卫生和生物工程等领域的科学。
3.细菌:是一类细胞细短、结构简单、胞壁坚韧、多以二分裂方式繁殖和水生性较强的原核生物。
4.细胞壁:位于细胞最外的一层厚实、坚韧的外被,主要成分为肽聚糖,具有固定细胞外形和保护细胞不受损伤等多种生理功能。
5.原生质体:指在人为条件下,用溶菌酶除尽原有细胞壁或用青霉素抑制新生细胞壁的合成后,所得到的仅有一层细胞膜包裹的圆球状渗透敏感细胞。
6.细胞质:是指被细胞膜包围的除核区以外的一切半透明、胶体状、颗粒状物质的总称。
7.核区:指原核生物所特有的无核膜包裹、无固定形态的原始细胞核。
(又称核质体、原核、拟核或核基因组)8.糖被:包被于某些细菌细胞壁外的一层厚度不定的透明胶体物质。
9.荚膜:糖被的一种,包裹在细菌细胞壁外,有固定层次的胶黏物,一般成分为多糖、少数为多肽或多糖与肽的复合物。
10.鞭毛:生长在某些细菌表面的长丝状、波曲的蛋白质附属物。
(具有运动功能)11.芽孢:某些细菌在其生长发育后期,细胞内形成一个圆形或椭圆形、厚壁、含水量低、抗逆性强的休眠构造,无繁殖功能。
12.孢囊:是一些固氮菌在外界缺乏营养的条件下,由整个营养细胞外壁加厚、细胞失水而形成的一种抗干旱但不抗热的圆形休眠体。
不具繁殖功能。
13.伴孢晶体:少数芽孢杆菌,在形成芽孢的同时,会在芽孢旁形成一颗菱形、方形或不规则形的碱溶性蛋白质晶体。
14.二分裂:一个细胞在其对称中心形成一隔膜,进而分裂成两个形态、大小和构造完全相同的子细胞。
15.菌落:在适宜的培养条件下,微生物在固体培养基上以母细胞为中心的一堆肉眼可见的,具有一定形态、构造等特征的子细胞集团。
16.放线菌:是一类主要呈菌丝状生长和以孢子繁殖的陆生性较强的原核生物。
微生物名词解释

—、名词解释1.normal flora:即正常菌群,在人的体表或与外界相通的腔道存在着不同种类和一定数量的细菌,通常对人有益无害,故称正常菌群.2.disinfection:即消毒,杀死病原微生物的方法.3.bacterium:即细菌,是一种体积微小,结构简单,具有原始细胞核和不完善的细胞器的单细胞的原核细胞型微生物.4.肽聚糖:细菌细胞壁的成分,由聚糖骨架、四肽侧链及五肽交连桥(革兰阳性菌具有)构成.5.转导:以温和噬菌体为媒介,将供体菌的一段DNA转移到受体菌内,使受体菌获得新的性状.6.细菌L型:在青霉素或溶菌酶作用下,细菌细胞壁的肽聚糖被破坏或合成受抑制,而形成的细胞壁缺陷型细菌,因最先在Lister研究所发现,故称.7.热原质:细菌合成的一种注入人体或动物体内能引起发热反应的物质.8.菌落:平板培养基上,单个细菌生长后形成的肉眼可见的细菌集团.9.噬菌体:感染细菌、真菌、放线菌和螺旋体等微生物的病毒.10.高压蒸汽灭菌法:在103.43kPa蒸汽压下,温度达到121.3℃,维持15~20min,可杀灭包括细菌芽胞在内的所有微生物.常用于耐高温、耐湿物品的灭菌.11.异染颗粒:白喉棒状杆菌的胞质中存在的胞质颗粒,用特殊染色后,可呈现与菌体明显不一样的颜色,称为异染颗粒.12.卡介苗:Calmette和Guérin将牛结核分枝杆菌经1.3年2.30次传代培养而获得的减毒活疫苗株,用于预防结核病.13.卫星现象:将流感嗜血杆菌与金黄色葡萄球菌共同接种在兔血平板上,金黄色葡萄球菌菌落周围的流感嗜血杆菌菌落较大,离金葡菌菌落越远的越小,此现象称为卫星现象.14.血浆凝固酶试验:金黄色葡萄球菌产生的一种侵袭性酶,使血浆中的纤维蛋白原转化为纤维蛋白,引起血浆凝固.15.肥达反应:用已知伤寒沙门菌O抗原和H抗原,及引起副伤寒的甲型副伤寒沙门菌\肖氏沙门菌和希氏沙门菌H抗原的诊断菌液与受检血清做定量凝集试验,测定受检血清中有无相应抗体及其效价的试验.16.巧克力平板:即热血平板,血平板加热80℃1.5min而制成,因其呈巧克力色而得名.17.串珠试验:炭疽芽胞杆菌在含微量青霉素的培养基上,其形态变异为大而均匀的圆球形,呈串珠状排列.是炭疽芽胞杆菌的重要鉴定试验.18.医院内感染:一切在医院内活动的人群(主要为住院病人)在医院内或病人在住院期间或出院后不久发生的感染以及与前次住院有关的感染.19.标准菌株:具有某种细菌典型而稳定的生物学特征且被国际认可的菌株.20.病毒包涵体:病毒感染细胞后,在胞浆或胞核内出现的嗜酸性或嗜碱性的包块.21.内基小体:狂犬病毒在易感动物或人的中枢神经细胞中增殖时,在胞质中形成的嗜酸性包涵体.22.干扰现象:两种病毒感染同一细胞时,会出现一种病毒抑制另一种病毒增殖的现象.23.CPE:即细胞病变效应,病毒感染细胞后,细胞所出现的浓缩、深染、胞质不均匀等一系列变化.24.Dane颗粒:具有感染性的完整的乙肝病毒颗粒.25.支原体:无细胞壁,能通过滤菌器,可人工培养的最小最简单的原核细胞型微生物.26.显微镜凝集试验:以标准的活钩体作为抗原,与不同稀释度的患者血清孵育,暗视野显微镜检查有无凝集现象.27.双相型真菌:在沙氏培养基中2.5℃培养形成丝状菌落,37℃培养形成酵母样菌落的一类真菌.28.conditioned pathegen:即条件致病菌,通常不致病,在特定条件引起感染的细菌.29.sterilization:即灭菌,杀死所有微生物的方法,包括病原微生物和非病原微生物,繁殖体和芽苞.30.侵袭力:病原菌突破机体的防御机能,在体内定居、生长繁殖、蔓延扩散的能力.31.LPS:即脂多糖,G-菌细胞壁特有的成分,由脂质A,核心多糖和特异性多糖组成,是细菌的内毒素.32.转化:受体菌直接摄取供体菌游离的DNA片段而获得新的性状.33.细菌:是一种体积微小,结构简单,具有原始细胞核和不完善的细胞器的单细胞的原核细胞型微生物.34.细菌素:细菌所产生的能抑制同种细菌增殖的蛋白质.35.专性厌氧菌:只能在无氧条件生长的细菌.36.噬菌体:感染细菌、真菌、放线菌和螺旋体等微生物的病毒.37.芽胞:细菌细胞壁外包围的一层由疏水性多糖或蛋白质多聚体组成的黏液性物质结构.38.ASO:SLO抗原性强,链球菌侵入体内产生SLO, 2~3周刺激机体产生相应抗体ASO,维持数月至1年,其效价>1:400可做为风湿热及其活动性的辅助诊断.39.BCG:即卡介苗,Calmette和Guérin将牛分枝杆菌经13年230次传代而获得的减毒活疫苗株,用于预防结核病.40.迁徙生长现象:变形杆菌在固体培养基上呈扩散性生长,形成以菌接种部位为中心的厚薄交替、同心圆形的层层波状菌苔.41.非发酵菌:一大群不发酵葡萄糖或仅以氧化形式利用葡萄糖的无芽胞的G-杆菌.42.汹涌发酵现象:产气荚膜梭菌能分解牛乳培养基中的乳糖产酸,使其中的酪蛋白凝固;同时产生大量气体,可将凝固的酪蛋白冲成蜂窝状,把液面凝固的凡士林层向上推,甚至冲走试管塞,称为汹涌发酵现象.43.热血平板:即巧克力平板,血平板加热80℃15min而制成.44.Ascol热沉淀反应:炭疽芽胞杆菌的菌体多糖抗原耐热,耐腐败,在病兽腐败脏器或皮毛经长时间煮沸仍可与相应血清发生沉淀反应.45.inclusion body:即包涵体,病毒感染细胞后,在胞浆或胞核内出现的嗜酸性或嗜碱性的包块.46.Negri body:即内基小体,狂犬病毒在易感动物或人的中枢神经细胞中增殖时,在胞质中形成的嗜酸性包涵体.47.病毒的灭活:病毒失去感染性.48.Antigenic drift:抗原发生小的变异49.干扰素:病毒或其他干扰素诱生剂刺激人或动物细胞所产生的一种分泌性蛋白,具有抗病毒、抗肿瘤和免疫调节作用.50.HA:即血凝素,流感病毒包膜上的刺突,具有凝集红细胞的作用.51.逆转录病毒:RNA病毒在复制时,通过DNA中间体,并与细胞染色体整合.52.螺旋体:一类细长柔软弯曲呈螺旋状的原核细胞型微生物.53.朊粒:仅存在于感染动物的组织中、具有致病性与传染性、对蛋白酶K有抗性的特殊蛋白质.54.孢子:真菌的繁殖结构,由生殖菌丝产生.55.外毒素:主要由G+菌和部分G-菌产生并释放到菌体外的毒性蛋白质.56.disinfection:即消毒,杀死病原微生物的方法.57.plasmid:即质粒,细菌染色体外的遗传物质,存在细胞质中的环状DNA分子58.荚膜:细菌细胞壁外包围的一层由疏水性多糖或蛋白质多聚体组成的黏液性物质结构.具有抗吞噬、粘附及抗有害物质损伤的作用.59.接合:是细菌通过性菌毛相互连接沟通,将遗传物质(主要是质粒DNA)从供体菌转移给受体菌.60.中介体:部分细胞膜内陷、折叠、卷曲形成的囊状物,与细菌分裂有关,为细菌提供大量能量.61.吲哚试验:有些细菌如大肠埃希菌,能分解培养基中的色氨酸生成无色的吲哚,其可与对半-氨基苯甲醛反应生成红色的玫瑰吲哚.62.细菌:是一种体积微小,结构简单,具有原始细胞核和不完善的细胞器的单细胞的原核细胞型微生物.63.phage:即噬菌体,感染细菌、真菌、放线菌和螺旋体等微生物的病毒.64.条件致病菌:通常不致病,在特定条件引起感染的细菌.65.SPA:即葡萄球菌A蛋白,金黄色葡萄球菌细胞壁的成分,能与IgG的Fc段特异性结合,具有抗吞噬的作用.66.acid-fast bacilli:即抗酸杆菌,分枝杆菌的细胞壁含分枝菌酸,使其不易着色,而一旦着色又具有抗酸酒精脱色的能力,故称.67.metachromatic granules:即异染颗粒,白喉棒状杆菌的胞质中存在的胞质颗粒,用特殊染色后,可呈现与菌体明显不一样的颜色,故称.68.coagulase:即凝固酶,金黄色葡萄球菌产生的一种侵袭性酶,使血浆中的纤维蛋白原转化为纤维蛋白,引起血浆凝固.69.Widal’s test:即肥达反应,用已知伤寒沙门菌O抗原和H抗原,及引起副伤寒的甲型副伤寒沙门菌\肖氏沙门菌和希氏沙门菌H抗原的诊断菌液与受检血清做定量凝集试验,测定受检血清中有无相应抗体及其效价的试验.70.Ascoli 热沉淀试验:炭疽芽胞杆菌的菌体多糖抗原耐热,耐腐败,在病兽腐败脏器或皮毛经长时间煮沸仍可与相应血清发生沉淀反应.71.串珠试验:炭疽芽胞杆菌在含微量青霉素的培养基上,其形态变异为大而均匀的圆球形,呈串珠状排列.是炭疽芽胞杆菌的重要鉴定试验.72.Weil-Felix test:即外裴试验,立克次体与变形杆菌的某些X菌株有共同抗原,故可用这些X 菌株代替立克次体抗原进行非特异性凝集反应以检测抗体,辅助诊断立克体病.73.standard strain:即标准菌株,具有某种细菌典型而稳定的生物学特征且被国际认可的菌株.74.原体:衣原体的细胞外存在形式,小而致密,具有感染性.75.interference:即干扰现象,两种病毒感染同一细胞时,会出现一种病毒抑制另一种病毒增殖的现象.76.inactivation:即灭活,病毒失去感染性.77.垂直传播:病毒从亲代传给子代.78.NA:即神经氨酸酶,流感病毒包膜上的刺突,能作用于细胞表面糖蛋白末端的神经氨酸,有利于病毒的成熟释放.79.Mycoplasma:即支原体,无细胞壁,能通过滤菌器,可人工培养的最小最简单的原核细胞型微生物.80.病毒体:完整成熟的病毒颗粒.81.抗原性转变:抗原发生大的变异,是质变,多产生新的型别.61.鞭毛:细菌体上附着的细长呈波状弯曲的丝状物,是细菌的运动器官.62.荚膜:细菌细胞壁外围绕的粘性、胶胨样物质,厚度在0.2μm上者,称为荚膜.63.芽胞:有些细菌在一定条件下,在菌体内形成一折光性很强的小体,有强大的抵抗力.64.L型细菌:无细胞壁或细胞壁有缺陷的细菌.65.噬菌体:是侵袭细菌、真菌或螺旋体的病毒,在易感的宿主细胞内增殖并将其裂解.66.质粒:存于细菌染色体外的环形DNA.67.条件致病菌:寄居在人体一定部位的细菌,在正常情况下不致病,当机体平衡状态破坏时可引起疾病者.68.外毒素:细菌在生长繁殖过程中分泌到菌体外的毒性物质.69.内素素:革兰氏阴性细菌的细胞壁外部结构,当菌体死亡或裂解后才释出的毒性物质.70.甲型溶血:指在血液琼脂平板上,细菌菌落周围的草绿色溶血环.71.乙型溶血:指在血液琼脂平板上,细菌菌落周围的无色透明溶血环.72.异染颗粒:指白喉杆菌菌体的染色性不均匀而出现的染色较深的颗粒.73.菌体抗原:是细菌的细胞壁成分,有抗原性、耐热与相应抗体呈颗粒状凝集.74.鞭毛抗原 :细菌的鞭毛蛋白,有抗原性、不耐热,与相应的抗体呈絮状凝集.75.表面抗原:细菌体周围的多糖成份,不耐热,可阻断0抗原与相应抗体反应.76.ETEC:产肠毒素型大肠埃希氏菌.77.EPEC:致病性大肠埃希氏茵.78.EIEC:侵袭性大肠埃希氏菌.79.非发酵菌:指一大群不发酵糖类、专性需氧、无芽胞的革兰氏阴性杆菌.80.卫星现象:指流感嗜血杆菌生长在金葡萄周围处的菌落增大现象.。
微生物学名词解释

微生物(Microorganism):指一般肉眼看不见或看不清的微小生物的总称。
微生物学(Microbiology):是研究微生物生命活动规律的科学。
微生物生态学:是研究微生物与其周围生物和非微生物环境之间相互关系的一门科学。
原核微生物(prokaryote):细胞核无核膜包裹,只存在由裸露DNA组成的核区的原始单细胞生物,包括细菌、古菌、放线菌、蓝细菌等。
真核微生物(eukaryote):是具有核膜、核仁分化,能进行有丝分裂的一类较高等的微生物,其细胞质中有线粒体等细胞器和内质网等内膜结构,包括酵母菌、霉菌、单细胞藻类、原生生物等。
细菌(bacteria):指原核微生物中非古菌类的主要类群。
脂多糖(lipopolysaccharide/LPS):是革兰氏阴性菌细胞壁特有的成分,由O-侧链、核心多糖和类脂A三部分组成。
周质空间(periplasmicspace)或壁膜空间:在革兰氏阴性细菌中,位于细胞壁与细胞质膜之间的狭小空间。
原生质体(protoplast):在革兰氏阳性细菌培养物中加入溶菌酶或青霉素阻止其细胞壁的正常合成而获得的完全缺壁细胞。
原生质球(spheroplast):指细胞壁未全部去掉的细菌细胞,呈圆球状,可人为地通过溶菌酶或青霉素处理革兰氏阳性细菌而获得。
细菌L-型(L-form of bacteria):是细菌在某些环境条件下因基因突变而产生的无壁类型。
荚膜(capsule):有些细菌生活在一定营养条件下,可向细胞壁外分泌出一层黏性多糖。
鞭毛(flagella):某些细菌的细胞表面伸出细长、波曲、毛发状的附属物。
芽孢(spore):某些细菌在其生活史的一定阶段,于营养细胞内形成一个圆形、椭圆形或圆柱形的结构。
菌落(colony):细菌在固体培养基上生长发育,几天内即可由1个或几个细菌分裂繁殖聚集而形成肉眼可见的群体。
纯培养(pure culture):如果一个菌落是由一个细菌个体生长、繁殖而成,则称为纯培养。
微生物名词解释

四、名词解释1. 微生物:是一切肉眼看不见或看不清的微小生物的总和。
(个体微小、结构简单、进化地位低,必须借助显微镜才能看清的微小生物的总称)1.基内菌丝;2.细菌菌落和菌苔;3.质粒;4.芽孢和孢囊;5.革兰氏染色法;6.伴孢晶体;7.荚膜;8.球状体(原生质球);9.古细菌; 10.L型细菌1.生长在固体培养基内,主要功能为吸收营养物,故亦称营养菌丝。
2.细菌在固体培养基上生长发育,几天即可由一个或几个细胞分裂繁殖聚集在一起形成肉眼可见的群体,称为细菌菌落。
许多菌落相互联接成一片称菌苔。
3.质粒是细菌染色体以外的遗传物质,能独立复制,为共价闭合环状双链DNA,分子量比染色体小,每个菌体内有一个或几个质粒,它分散在细胞质中或附着在染色体上。
4.某些细菌,在其生长的一定阶段,细胞内形成一个圆形.椭圆形或圆柱形的结构,对不良环境条件具有较强抗性的休眠体称芽孢。
有些细菌由营养细胞缩短变成球形,表面形成一层厚的孢壁,称为孢囊。
5.丹麦科学家Gram十九世纪八十年代发明的一种细菌染色法。
染色方法为:在一个已固定的细菌涂片上用结晶紫染色,再加媒染剂---碘液处理,使菌体着色,然后用乙醇脱色,最后用蕃红复染。
显微镜下菌体呈紫色者为G+细菌,菌体呈红色者为G-细菌。
6.指少数产芽孢细菌,例如苏云金芽孢杆菌(Bacillus thuringiensis)在其形成芽孢的同时,会在芽孢旁形成一颗菱形或双锥形的碱溶性蛋白晶体——δ内毒素,即为伴孢晶体。
7.指一些细菌生活在一定营养条件下,向细胞壁外分泌出一层黏滞性较大.相对稳定地附着在细胞壁外.具一定外形.厚约200nm的黏性物质。
8.用人工方法部分除去细菌细胞壁后剩下的细菌细胞称球状体。
一般由G-细菌形成。
9.指在细胞壁组成.细胞膜组成.蛋白质合成的起始氨基酸.RNA聚合酶的亚基数等方面与真细菌有明显差异的原核生物。
包括:产甲烷古细菌群.还原磷酸盐的古细菌群.极端嗜盐的古细菌群等。
微生物名词解释

◎第1章1.微生物(mi cr oor g an is m):通常描述一切不借助显微镜用肉眼看不见的微小生物。
这类微生物包括病毒、细菌、古菌、真菌、原生生物和某些藻类。
2.微生物学(m icr o bi ol og y):指大量的、极其多样的、不借助显微镜看不见的微小生物类群的总称。
微生物通常包括病毒、亚病毒(类病毒、拟病毒、朊病毒)、具原核细胞结构的真细菌、古生菌以及具真核细胞结构的真菌(酵母、霉菌)、原生动物和单细胞藻类。
3.分子微生物学(mo le cu la r mi cro b io lo gy):在分子水平上研究微生物生命活动规律的科学。
4.细胞微生物学(ce ll ul ar mi cr ob i ol og y):重点研究微生物与寄主细胞相互关系的科学。
5.微生物基因组学(mi cr ob ic g eno m ic s):研究微生物基因组的分子结构、信息含量及其编码的基因产物的科学。
6.自生说(s po nta n eo us ge ne ra ti on):一个古老的学说,认为一切生命有机体都能够从无生命的物质自然发生。
7.安东·列文虎克(An to ny va n Le e uw en ho ek,1632-1723):荷兰商人,他是真正看见并描述微生物的第一人,他利用自制放大倍数为50~300倍的显微镜发现了微生物世界(当时称之为微小动物),首次揭示了一个崭新的生物世界――微生物界。
8.路易斯·巴斯德(Lo ui s Pa st eur,1822-1895):法国人,原为化学家,后来转向微生物学研究领域,为微生物学的建立和发展作出了卓越的贡献,成为微生物学的奠基人。
主要贡献:用曲颈瓶实验彻底否定了“自生说”,从此建立了病原学说,推动了微生物学的发展;研究了鸡霍乱,发现将病原菌减毒可诱发免疫性,以预防鸡霍乱病,其后他又研究了牛、羊炭疽病和狂犬病,并首次制成狂犬疫苗,证实其免疫学说,为人类防病、治病作出了重大贡献;分离到了许多引起发酵的微生物,并证实乙醇发酵是由酵母菌引起的,也发现乳酸发酵、醋酸发酵和丁酸发酵是由不同细菌所引起的,为进一步研究微生物的生理生化和工业微生物学奠定了基础。
微生物学名词解释

微生物学名词解释微生物名词解释微生物:指所有个体微小、结构简单的低等生物的总称。
微生物学:是一门在细胞、分子或群体水平上研究微生物的形态构造、生理代谢、遗传变异、生态分布和分类进化等生命活动基本规律,并将其应用于工业发酵、医药卫生、生物工程和环境保护等实践领域的科学,其根本任务是发掘、利用、改善和保护有益微生物,控制、消灭或改造有害微生物,为人类社会的进步服务。
细菌质粒:一般是指存在于细菌细胞质中的一类能自我复制的小型双链闭合环状DNA分子。
失去质粒一般不影响细胞存活,但会失去质粒携带的遗传信息。
目前在真核微生物中也有发现,也发现有RNA质粒。
间体:是细胞膜内褶形成的一种管状、层状或囊状结构。
荚膜:细菌的特殊结构。
是指覆盖在某些细菌细胞壁外、有明显界限的一层胶状物质,主要成分为水以及多糖或多肽。
芽孢:某些细菌在其生长发育后期在细胞内所形成的一个圆形或椭圆形的抗逆性休眠体。
伴胞晶体:少数芽孢杆菌在其形成芽孢的同时,在细胞内形成的一种菱形或双椎形碱溶性蛋白晶体。
伴胞晶体对昆虫尤其是鳞翅目昆虫的幼虫有毒杀作用。
菌落:将单个微生物细胞或一小堆同种细胞接种在固体造就基上,在相宜的造就条件下迅速生长繁殖,形成以母细胞为中央的一堆肉眼可见的、有一定形态的仔细胞集团,称为菌落。
菌苔:如果将某一纯种的大量细胞麋集地接种到固体造就基外表,结果长成的“菌落”相互连接成一片,即为菌苔。
L型细菌:一种自发突变形成的细胞壁缺损细菌。
它的细胞膨大,对渗透压敏感,在固体外表形成“油煎蛋”似的小菌落。
原生质体:用人工方法除去细菌细胞壁后,剩下的完全缺壁的细胞叫原生质体,一般由G+细菌形成球状体:用人工方法局部除去细菌细胞壁后剩下的细菌细胞称球状体,一般由G-细菌形成。
疵壁菌:嗜盐菌、产甲烷菌等古生菌的细胞壁中不含有典型的肽聚糖成分,被称为疵壁菌。
菌胶团:有的细菌,它们的荚膜物质互相融合在一起成一团胶状物,其内常包含有多个菌体,称菌胶团。
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GlossaryActinomycetes(放线菌,放线菌属) A group of filamentous, funguslike bacteria. active transport (主动运输) Nutrient transport method that requires carrier proteins in the membranes of the living cells and the expenditure of energy. adenovirus Noenveloped DNA virus; means of transmission is human-to-human via respiratory and ocular secretions.aerobe(需氧微生物)A microorganism that lives and grows in the presence of free).gaseous oxygen (O2aerobic respiration(需氧呼吸)Respiration in which the final electron acceptor in the electron transport chain is oxygen (O).2agar(琼脂)A polysaccharide found in seaweed and commonly used to prepare solid culture media.AIDS(艾滋病)Acquired immunodeficiency syndrome. The complex of signs and symptoms characteristic of the late phase of human immunodeficiency virus (HIV) infection.algae(藻类) Photosynthetic, plant-like organisms which generally lack the complex structure of plants; they may be single-celled or multicellular, and inhabit diverse habitats such as marine and freshwater environments, glaciers, and hot springs.Ames test(艾姆斯氏试验)A method for detecting mutagenic and potentially carcinogenic agents based upon the genetic alteration of nutritionally defective bacteria.ammonification(氨化作用) Phase of the nitrogen cycle in which ammonia is released from decomposing organic material.anaerobe(厌氧性微生物,厌氧菌) A microorganism that grows best, or exclusively, in the absence of oxygen.anaerobic respiration(无氧呼吸)Respiration in which the final electron acceptor in the electron transport chain is an inorganic molecule containing sulfate, nitrate, nitrite, carbonate, etc.antagonism(拮抗作用)Relationship in which microorganisms compete for survival in a common environment by taking actions that inhibit or destroy another organism.antibiotic(抗生素)A chemical substance from one microorganism that can inhibit or kill another microbe even in minute amounts.archaea(古生菌) Prokaryotic single-celled organisms of primitive origin that have unusual anatomy, physiology and genetics, and live in harsh habitats;when capitalized (Archaea) the term refers to one of the three domains of living organisms as proposed by Woese.arthrospore(分节孢子)A fungal spore formed by the septation and fragmentation of hyphae.ascospore (囊孢子,子囊孢子)A spore formed within a saclike cell (ascus) of Ascomycota following nuclear fusion and meiosis.ascus(子囊) Special fungal sac in which haploid spores are created. autoclave(高压锅) A sterilization chamber which allows the use of steam under pressure to sterilize materials. The most common temperature/pressure combination for an autoclave is 121℃and 15 psi.autotroph(自养生物) A microorganism that requires only inorganic nutrients and whose sole source of carbon is carbon dioxide.Bacillus(芽孢杆菌) Bacterial cell shape that is cylindrical (longer than it is wide).back-mutation(回复突变) A mutation which counteracts an earlier mutation, resulting in the restoration of the original DNA sequence.bacteria (细菌)Category of prokaryotes with peptidoglycan in their cell wails and a single, circular chromosome. This group of small cells is widely distributed in the earth’s habitats.Bacteria (plural of bacterium) When capitalized can refer to one of the three domains of living organisms proposed by Woese, containing all non- archaea prokaryotes.bacterial chromosome 细菌染色体A circular body in bacteria that contains the primary genetic material. Also called nucleoid.Bacteriophage噬菌体A virus that specifically infects bacteria.bacterium 杆菌属A tiny unicellular prokaryotic organism that usually reproduces by binary fission and usually has a peptidoglycan cell wall, has various shapes, and can be found in virtually any environment.basidiospore(芽孢) A sexual spore that arises from a basidium. Found in basidiomycota fungi.Bdellovibrio(蛭弧菌属) A bacterium that preys on certain other bacteria. It bores a hole into a specific host and inserts itself between the protoplast and the cell wall. There it elongates before subdividing into several cells and devouring the host cell.capsule蒴果In bacteria, the loose, gel-like covering or slime made chiefly of simple polysaccharides. This layer is protective and can be associated with virulence.Chemoautotroph华能自养生物An organism that relies upon inorganic chemicals for its energy and carbon dioxide for its carbon. Also called a chemolithotroph. chemoheterotroph华能异养生物Microorganisms that derive their nutritional needs from organic compounds.chemotaxis趋化性The tendency of organisms to move in response to a chemical gradient (toward an attractant or to avoid adverse stimuli).chitin 几丁质A polysaccharide similar to cellulose in chemical structure. This polymer makes up the horny substance of the exoskeletons of arthropods and certain fungi.chromosome染色体The tightly coiled bodies in cells that are the primary sites of genes.Clone无性繁殖 A colony of cells (or group of organisms) derived from a single cell (or single organism) by asexual reproduction. All units share identical characteristics. Also used as a verb to refer to the process of producinga genetically identical population of cells or genes.colony菌落 A macroscopic cluster of cells appearing on a solid medium, each arising from the multiplication of a single cell.commensalism共栖An unequal relationship in which one species derives benefit without harming the other.conjugation结合作用In bacteria, the contact between donor and recipient cells associated with the transfer of genetic material such as plasmids. Can involve special (sex) pili. Also a form of sexual recombination in ciliated protozoans.death phase死亡期End of the cell growth due to lack of nutrition, depletion of environment, and accumulation of wastes. Population of cells begins to die.decomposition分解The breakdown of dead matter and wastes into simple compounds, that can be directed back into the natural cycle of living things. diffusion扩散The dispersal of molecules, ions, or microscopic particles propelled down aconcentration gradient by spontaneous random motion to achieve a uniform distribution.diploid 二倍体Somatic cells having twice the basic chromosome number. One set in the pair is derived from the father, and the other from the mother. direct, or total cell count 细胞计数1. Counting total numbers of individual cells being viewed with magnification. 2. Counting isolated colonies of organisms growing on a plate of media as a way to determine population size. endospore内生孢子A small, dormant, resistant derivative of a bacterial cell that germinates under favorable growth conditions into a vegetative cell.The bacterial genera Bacillus and Clostridium are typical sporeformers. eubacteria 真细菌Term used for non-archaea prokaryotes, stands for "true bacteria"eukaryotic cell真核细胞A cell that differs from a prokaryotic cell chiefly by having a nuclear membrane (a well-defined nucleus), membrane-bounded subcellular organelles, and mitotic cell division.Eukarya真核生物One of the three domains (sometimes called superkingdoms) of living organisms, as proposed by Woese; contains all eukaryotic organisms. eutrophication富营养化The process whereby dissolved nutrients resulting from natural seasonal enrichment or industrial pollution of water cause overgrowth of algae and cyanobacteria to the detriment of fishand other large aquatic inhabitants.fermentation 发酵The extraction of energy through anaerobic degradation of substrates into simpler, reduced metabolites. In large industrial processes, fermentation can mean any use of microbial metabolism to manufacture organic chemicals or other products.fimbria 菌毛A short, numerous surface appendage on some bacteria that provides adhesion but not locomotion.flagellum鞭毛A structure that is used to propel the organism through a fluid environment.gene基因A site on a chromosome that provides information for a certain cell function. A specific segment of DNA that contains the necessary code to makea protein or RNA molecule.generation time代时Time required for a complete fission cycle-from parent cell to two new daughter cells. Also called doubling time.genome 基因组染色体组The complete set of chromosomes and genes in an organism. Genotype 基因型 The genetic makeup of an organism. The genotype is ultimately responsible for an organism's phenotype, or expressed characteristics. Gram stain革兰氏染色剂 A differential stain for bacteria useful in identification and taxonomy. Gram-positive organisms appear purple from crystal violet- mordant retention, whereas gram-negative organisms appear red after loss of crystal violet and absorbance of the safranin counterstain. growth curve生长曲线A graphical representation of the change in population size over time. This graph has four periods known as lag phase, exponential or log phase, stationary phase, and death phase.growth factor生长因子An organic compound such as a vitamin or amino acid that must be provided in the diet to facilitate growth. An essential nutrient. halophile好盐菌A microbe whose growth is either stimulated by salt or requiresa high concentration of salt for growth.haploid 单倍体Having a single set of unpaired chromosomes, such as occurs in gametes and certain microbes.host 宿主Organism in which smaller organisms or viruses live, feed, and reproduce.human immunodeficiency virus (HIV)艾滋病病毒A retro virus that causes acquired immunodeficiency syndrome (AIDS).intron 内含子The segments on split genes of eucaryotes that do not code for polypeptide. They can have regulatory functions. See exon.in vitro离体 Literally means "in glass," signifying a process or reaction occurring in an artificial environment, as in a test tube or culture medium. in vivo生物体内Literally means “in a living being,” signifying a process or reaction occurring in a living thing.Koch’s postulates科赫法则 A procedure to establish the specific cause of disease. In all cases of infection: ( 1 ) The agent must be found; (2) inoculations of a pure culture must reproduce the same disease in animals;(3) the agent must again be present in the experimental animal: and (4) apure culture must again be obtained.lag phase延迟期The early phase of population growth during which no signs ofgrowth occur.L form 缺壁细菌 L-phase variants; wall-less forms of some bacteria that are induced by drugs or chemicals. These forms can be involved in infections. lipopolysaceharide 脂多糖 A molecular complex of lipid and carbohydrate found in the bacterial cell wall. The lipopolysaccharide (LPS) of gram-negative bacteria is an endotoxin with generalized pathologic effects such as fever. log phase指数期Maximum rate of cell division during which growth is geometricin its rate of increase. Also called exponential growth phase.Meiosis 减数分裂 The type of cell division necessary for producing gametes in diploid organisms. Two nuclear divisions in rapid succession produce four gametocytes, each containing a haploid number of chromosomes.minimum inhibitory concentration (MIC) 最低抑菌浓度The smallest concentration of drug needed to visibly control microbial growth.minimum temperature 最低温度 The lowest temperature at which an organism will grow.missense mutation错义突变 A mutation in which a change in the DNA sequence results in a different amino acid being incorporated into a protein, with varying results.mitosis 有丝分裂Somatic cell division that preserves the somatic chromosome number.mycelium 菌丝菌丝体The filamentous mass that makes up a mold. Composed of hyphae.Mycorrhizae 菌根Various species of fungi adapted in an intimate, mutualistic relationship to plant roots.nitrification硝化作用Phase of the nitrogen cycle in which ammonium is oxidized. nitrogen fixation固氮作用 A process occurring in certain bacteria in which atmospheric Ngas is converted to a form (NH4) usable by plants.2nonsense mutation 无义突变A mutation that changes an amino acid-producing codon into a stop codon, leading to premature termination of a protein. nucleus 细胞核The central core of an atom, composed of protons and neutrons. obligate专性Without alternative; restricted to a particular characteristic.Example: An obligate parasite survives and grows only in a host; an obligate aerobe must have oxygen to grow; an obligate anaerobe is destroyed by oxygen. optimum temperature最适温度The temperature at which a species shows the most rapid growth rate.parasite 寄生虫An organism that lives on or within another organism (the host), from which it obtains nutrients and enjoys protection. The parasite produces some degree of harm in the host.passive transport 被动运输Nutrient transport method that follows basic physical laws and does not require direct energy input from the cell. pasteurization 巴斯消毒法Heat treatment of perishable fluids such as milk, fruit juices, or wine to destroy heat-sensitive vegetative cells, followed by rapid chilling to inhibit growth of survivors and germination of spores. It prevents infection and spoilage.pathogen 病原体Any agent, usually a virus, bacterium, fungus, protozoan, or helminth, that causes disease.phage 噬菌体A bacteriophage; a virus that specifically parasitizes bacteria. phenotype表型The observable characteristics of an organism produced by the interaction between its genetic potential (genotype) and the environment. photoautotroph 光合自养生物An organism that utilizes light for its energy and carbon dioxide chiefly for its carbon needs.pill Small, stiff filamentous appendages in gram-negative bacteria that functionin DNA exchange during bacterial conjugation.plasmids质粒 Extrachromosomal genetic units characterized by several features.A plasmid is a double-stranded DNA that is smaller than and replicatesindependently of the cell chromosome; it bears genes that are not essential for cell growth; it can bear genes that code for adaptive traits; and it is transmissible to other bacteria.polysaccharide多糖 A carbohydrate that can be hydrolyzed into a number of monosaccharides. Examples: cellulose, starch, glycogen.prion朊病毒A concocted word to denote “proteinaceous infectious agent”; a cytopathic protein associated with the slow-virus spongiform encephalopathies of humans and animals.prophage 原噬菌体 A lysogenized bacteriophage; a phage that is latently incorporated into the host chromosome instead of undergoing viral replication and lysis.protoplast原生质体A bacterial cell whose cell wall is completely lacking and that is vulnerable to osmotic lysis.protozoa 原生动物A group of single-celIed, eucaryotic organisms. pseudohypha 假菌丝A chain of easily separated, spherical to sausage-shaped yeast cells partitioned by constrictions rather than by septa.pure culture 纯培养A container growing a single species of microbe whose identity is known.Retrovirus逆转录酶病毒 A group of RNA viruses (including HIV) that have the mechanisms for converting their genorne into a double strand of DNA that can be inserted on a host’s chromosome.reverse transcriptase逆转录酶The enzyme possessed by retrovimses that carries out the reversion of RNA to DNA a form of reverse transcription. rhizobia 根瘤菌Bacteria that live in plant roots and supply supplemental nitrogen that boosts plant growth.rhizosphere 根际The zone of soil, complete with microbial inhabitants, in the immediate vicinity of plant roots.rickettsias 立克次体Medically important family of bacteria, commonly carried by ticks, lice, and fleas. Significant cause of important emerging diseases. root nodules 根瘤Small growths on the roots of legume plants that arise froma symbiotic association between the plant tissues and bacteria (Rhizobia).This association allows fixation of nitrogen gas from the air into a usable nitrogen source for the plant.silent mutation同义突变 A mutation that, because of tire degeneracy of the genetic code, results in a nucleotide change in both the DNA and mRNA but not the resultant amino acid and thus, not the protein.spore孢子 A differentiated, specialized cell form that can be used for dissemination, for adverse conditions, and/or for are usually unicellular and may develop into gametes or vegetative organisms.stationary growth phase 稳定期Survival mode in which cells either stop growing or grow very slowly.sterilization 灭菌Any process that completely removes or destroys all viablemicroorganisms, including viruses, from an object or habitat. Material so treated is sterile.strict, or obligate anaerobe严格厌氧微生物An organism which does not use oxygen gas in metabolism and cannot survive in oxygen’s presence.symbiosis共生An intimate association between individuals from two species; used as a synonym for mutualism.synergism协同作用The coordinated or correlated action by two or more drugs or microbes that results in a heightened response or greater activity. temperate phage 温和噬菌体A bacteriophage that enters into a less virulent state by becoming incorporated into the host genome as a prophage instead of in the vegetative or lytic form that eventually destroys the cell.template 模板The strand in a double stranded DNA molecule which is used as a model to synthesize a complementary strand of DNA or RNA during replication or transcription.thermal death point致死温度The lowest temperature that achieves sterilization in a given quantity of broth culture upon a 10-minute exposure. Examples: 55℃for Escherichia coli, 60℃ for Mycobacterium tuberculosis, and 120℃ for spores.thermal death time热致死时间The least time required to kill all cells of a culture at a specified temperature.thermophile 嗜热生物A microorganism that thrives at a temperature of 50℃ or higher.transcription转录mRNA synthesis; the process by which a strand of RNA is produced against a DNA template.transduction转导The transfer of genetic material from one bacterium to another by means of a bacteriophage vector.transformation转化In microbial genetics, the transfer of genetic material contained in “naked” DNA fragments from a donor cell to a competent recipient cell.transposon转位子A DNA segment with an insertion sequence at each end, enabling it to migrate to another plasmid, to the bacterial chromosome, or to a bacteriophage.Vibrio弧菌A curved, rod-shaped bacterial cell.virulence 毒性In infection, the relative capacity of a pathogen to invade and harm host cells.zygospore 接合孢子A thick-walled sexual spore produced by the zygomycete fungi.It develops from the union of two hyphae, each bearing nuclei of opposite mating types.。