高效液相色谱法测定五苓片中肉桂酸含量

高效液相色谱法测定五苓片中肉桂酸含量

袁祥慧;杨惠莲

【期刊名称】《中国药业》

【年(卷),期】2011(20)9

【摘要】目的建立测定五苓片中肉桂酸含量的高效液相色谱法.方法色谱柱采用Diamonsil C18柱(250mm× 4.6 mm,5μm),以乙腈-0.1%磷酸溶液(30:70)为流动相,流速1.0mL/min,检测波长285nm.结果肉桂酸进样量在0.009 608～0.288 24μg范围内与峰面积线性关系良好,r=0.9999(n=6);平均回收率为96.39%,RSD 为0.44%(n=6).结论高效液相色谱法专属性强、操作简便、结果准确、重现性好,可用于测定五苓片中肉桂酸的含量.%Objective To establish an RP- HPLC method for the determination of cinnamic acid in Wuling Tablets. Methods The RPHPLC method was adopted and Diam onsil C18 column(250 mm ×4.

6 mm,5 μm) was used. The mobile phase was acetonitrile-0. 1％ phosphric acid(30∶70) with the flow rate of 1.0 mL/min. The UV detection wavelength was set at 285 nm. Results The calibration curve of cinnamic acid was linear in the range of 0. 009 608 -0. 288 24 μg. The average recover rate was 96.39％, RSD =0. 44％ ( n =6). Conclusion The method is specific,simple,accurate and has good repeatability. It can be used to determinate the cinnamic acid in Wuling Tablets.

【总页数】2页(P18-19)

【作者】袁祥慧;杨惠莲

【作者单位】重庆市药品检验所,重庆,401121;重庆市药品检验所,重庆,401121【正文语种】中文

【中图分类】R282.41;R286.0

【相关文献】

1.五苓散、五苓胶囊、五苓片中肉桂与桂枝的使用情况分析 [J], 张幸福;周燕雪

2.高效液相色谱法测定七味柏苓片中盐酸小檗碱和五味子乙素的含量 [J], 谢晓燕;张忠君;于春英

3.高效液相色谱法测定脉络宁口服液中肉桂酸含量 [J], 邱星安

4.高效液相色谱法测定六味五灵片中齐墩果酸含量 [J], 黄其春;涂文升

5.高效液相色谱法测定桂龙咳喘宁胶囊中肉桂酸含量 [J], 解希田

因版权原因，仅展示原文概要，查看原文内容请购买