生物信息学作业

生物信息学第二次作业

（1）BLAST n

第一步：进入NCBI主页，然后在NCBI主页右边一栏点击BLAST，就进入了BLAST主页。第二步：进入NCBI主页后，先搜索nucleotide blast如下图：

第三步：进入后，输入要查找的序列信息，然后点击BLAST，如下图：

第四步：如下图操作，

第五步：出现结果，点击Formatting options如下图操作，

第六步：选择好需要下载的序列打钩后，点击download，如下图，

之后选择格式后点击continue，如下图，

下载结果如下,

>gi|635055167|ref|XM_007963592.1| PREDICTED: Chlorocebus sabaeus retinol binding protein 4, plasma (RBP4), transcript variant X2, mRNA TTGGCCTGCCCGAGGCCAACCCGCGCGGCCGCTCCACCGTGCCCGGGGCTGTCCTGG AGGTGAGGCCGGCCCACGGGGAC CCTGCCCACGCCCGGGCGCCGGGCGGA TTCCTGGGCAAGATGAAGTGGGTGTGGGCG CTCTTGCTGCTGGCGGCGCTGGG CAGTGGCCGGGCGGAGCGCGACTGCCGAGTGAGCAGCTTCCGAGTCAAGGAGAACTT CGACAAGGCTCGCTTCTCCGGGA

CCTGGTACGCCA TGGCCAAGAAGGACCCCGAGGGCCTCTTTCTGCAGGACAACATCGT CGCGGAGTTCTCCGTGGACGAG ACCGGCCAGATGAGCGCCACGGCCAAGGGCCGAGTCCGTCTTTTGAATAACTGGGAC GTGTGCGCAGACA TGGTGGGCAC CTTCACAGACACCGAGGACCCTGCCAAGTTCAAGA TGAAGTACTGGGGCGTAGCCTCC TTTCTCCAGAAAGGAAA TGATG ACCACTGGATCATCGACACGGACTACGACACGTATGCCGTGCAGTACTCCTGCCGCCT CCTGAACCTCGA TGGCACCTGT GCTGACAGCTACTCCTTCGTGTTTTCCCGGGACCCCAACGGCCTGCCCCCAGAAGCGC AAAAGATTGTAAGGCAGCGGCA GGAGGAGCTGTGCCTGGCCAGGCAGTACAGGCTGATCGTCCACAACGGTTACTGTGAT GGCAGA TCAGAAAGAAACCTTT

TGTAGCAAGATCAATAA TCTAGTTTCA TCTGAGAACTTCTGATTATTTCTCAGTCTTCAA CTCTATTTATCTTAGGAGTT TAATTTGCCCTTCTCTCCCCATCTTCCCTCATTTCCCA TAAAACCTTCA TTACACATAAGG ATACACGTGGGGGTCAGTG

AA TCTGCTTGCAGGTAAATGTCTTTCCTGAAAGTTTCTGAGGCTTAAGA TTCCAGACTC TGATTCATTAAAA TA TAGTCA

CCCGTGTCCTGTGA

>gi|795177333|ref|XM_011988487.1| PREDICTED: Mandrillus leucophaeus retinol binding protein 4, plasma (RBP4), mRNA

ATGGAGACACAACTGA TACTCGGCCGGGCGGAGCGCGACTGCCGAGTGAGCAGCTTC CGAGTCAAGGAGAACTTCGACAA GGCTCGCTTCTCCGGGACCTGGTACGCCATGGCCAAGAAGGACCCCGAGGGCCTCTTT CTGCAGGACAACA TCGTCGCGG

AGTTCTCCGTGGACGAGACCGGCCAGA TGAGCGCCACGGCCAAGGGCCGAGTCCGTC TTTTGAATAACTGGGACGTGTGC GCAGACATGGTGGGCACCTTCACAGACACCGAGGACCCTGCCAAGTTCAAGATGAAG TACTGGGGCGTAGCCTCCTTTCT

CCAGAAAGGAAA TGATGACCACTGGA TCATCGACACGGACTATGACACGTATGCCGTG CAGTACTCCTGCCGCCTCCTGA ACCTTGACGGCACCTGTGCTGACAGCTACTCCTTCGTGTTTTCCCGGGACCCCAACGG CCTGCCCCCAGAAGCGCAAAAG ATTGTAAGGCAGCGGCAGGAGGAGCTGTGCCTGGCCAGGCAGTACAGGCTGATCGTC CACAA TGGTTACTGTGA TGGCAG

ATCAGAAAGAAACCTTTTGTAGCAACATCAA TAA TCTAGTTTCA TCTGAGAACTTCTGA TTATTTCTCAGTCTTCAACTC

TA TTTATCTTAGGAGTTTAA TTTGCCCTTCTCTTCCCATCTTCCCTCGTTTCCCATAAAAC CTTCATTACACA TAAGGAT

ACACGTAGGGGTCAGTGAA TCTGCTTGCAGGTAAA TATCTTTCCTGAAAGTTTCTGAGG CTTAAGATTCCAGACTCTGAT

TCATTAAAATATAGTCACCCATGTCGTGTGA

>gi|795126907|ref|XM_011934356.1| PREDICTED: Colobus angolensis palliatus retinol binding protein 4, plasma (RBP4), mRNA

ATGGAGACACAACTGA TACTGTTGACTCGAGCCTCCTGCCAGAGCCACTGGCCCCGGA GGCCACCCCAGACCGCAGCTGG CGGCCGCTGGCACGAGTGCAGGGTCCCCACCCCTA TCCCCGCCCTCCCCGCGGCCCGC TGTGCGCCCCCGGACACCAAAC CCCTGCTGTTGTCATCCTTCTCACAGTTCTCGGGGACCTGGTACGCCATGGCCAAGAA GGACCCCGAGGGCCTCTTTCTG CAGGACAACATCGTCGCGGAGTTCTCCGTGGACGAGACCGGCCAGATGAGCGCCACG GCCAAGGGCCGAGTCCGTCTTTT

GAATAACTGGGACGTGTGTGCAGACA TGGTGGGCACCTTCACAGACACGGAGGACCC TGCCAAGTTCAAGATGAAGTACT GGGGCGTAGCCTCCTTTCTCCAGAAAGGAAA TGA TGACCATTGGATCATCGATACGGA CTACGACACGTA TGCCGTGCAG TACTCCTGCCGCCTCCTGAACCTCGACGGCACCTGTGCTGACAGCTACTCCTTCGTGTT TTCCCGGGACCCCAACGGCCT GCCCCCAGAAGCGCAAAAGATTGTAAGGCAGCGGCAGGAGGAGCTGTGCCTGGCCAG GCAGTACAGGCTGATCGTCCACA ACGGTTACTGCGATGGCAGATCAGAAAGAAACCTTTTGTAGCAACATCAAGAATCTAG TTTCATCTGAGAACTTCTGATT

ATTTCTCAGTCTTCAACTCTA TTTA TCTTAGGAGTTTAATTTGCCCTTCTCTCCCCA TCTT CCCTCATTTCCCATAAAAC

CTTCATTACACA TAAGGATACACGTGGGGTCAGTGAATCTGCTTGCAGTTAAATGTCTT TCCTGAAAGTTTCTGAGGCTT

AAGATTCCAGACTCTGATTCA TTAAAA TA TAGTCACCCGTGTCCTGTGA

>gi|544458882|ref|XM_005565973.1| PREDICTED: Macaca fascicularis retinol binding protein 4, plasma (RBP4), transcript variant X1, mRNA

TCCCCCCGGCGCTA TAAAGCAGCGGGGCGGCCGCTGCGCGCTGGCCTCACTAGCTCCA CGCGCGCCCGGACGCGGCGGCC AGGCTTGCGCGCGGTTCCCCTCCCAGTGGGCTGA TTCCTGGGCAAGATGAAGTGGGTG TGGGCGCTCTTGCTGCTGGCGG CGCTGGGCAGCGGCCGGGCGGAGCGCGACTGCCGAGTGAGCAGCTTCCGAGTCAAG GAGAACTTCGACAAGGCTCGCTTC

TCCGGGACCTGGTACGCCA TGGCCAAGAAGGACCCCGAGGGCCTCTTTCTGCAGGAC AACATCGTCGCGGAGTTCTCCGT GGACGAGACCGGCCAGATGAGCGCCACGGCCAAGGGCCGAGTCCGTCTTTTGAATAA CTGGGACGTGTGCGCAGACATGG