NEB酶切buffer
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Home > Technical Reference > Restriction Endonucleases > NEBuffer Activity Chart
NEBuffer Activity Chart for Restriction Enzymes
For your convenience, New England Biolabs offers a simple 4 buffer system. A color-coded 10X NEBuffer is supplied with every re
endonuclease, ensuring 100% activity. Over 170 restriction enzymes exhibit 100% activity in NEBuffer 4, resulting in increased eff and ease of use especially when performing double digests.
To help select the best conditions for double digests , this chart shows the optimal (supplied) NEBuffer and approximate activity in t
NEBuffers and NEBuffer EcoRI for each enzyme. If BSA is supplied with an enzyme, it is included in all NEBuffer activity reactions.
table shows recommended reaction temperature, heat inactivation temperature, recommended diluent buffer and whether the enzym
qualified (i.e., cleaves substrate in 5 minutes under recommended conditions). Note: The values listed in this table are approxima obtained using each enzyme's specific unit assay substrate DNA.
Please check out other technical reference information related to restriction enzymes:
Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enz
Click for the Chart Legend , Icon Description
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F | H | I | K | L | M | N | P | R | S | T | X | Z |
NEBuffer 4
NEBuffer 3NEBuffer 4NEBuffer 3
NEBuffer 4NEBuffer 4NEBuffer 4
NEBuffer 4NEBuffer 3NEBuffer 1
NEBuffer 4-
NEBuffer 4
NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 - NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4
NEBuffer 1 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 2 NEBuffer 4 NEBuffer 2 NEBuffer 1 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4
NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 2 NEBuffer 2 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 2 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4
NEBuffer 3
NEBuffer 4
NEBuffer 4
NEBuffer 2
NEBuffer 4
NEBuffer 2
NEBuffer 3
NEBuffer 3
NEBuffer 3
NEBuffer 3
NEBuffer 4
NEBuffer 4
NEBuffer 3
NEBuffer 4 Mix™-
NEBuffer 2
NEBuffer 4
NEBuffer 3
NEBuffer 2
NEBuffer 4
NEBuffer 3
NEBuffer 3
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 4
NEBuffer 3